Research powerpoint

1. Using Resistant Starches to
Inhibit Colon Cancer
Marissa Jackson
Jadyn Johnson
GWC Symposium
August 1, 2013

2. Objective
• To inhibit colon cancer using resistant
starches.
• Resistant Starches:
-not digested in the small intestine
-fermented in the large intestine
-nearly passes through digestion in the
small intestine

3. Resistant Starches

4. Hypothesis
• We hypothesize that resistant starch fed in a
diet will be able to inhibit a common
preneoplastic lesion, Aberrant Crypt Foci
(ACF).

5. ACF

6. Background
• Using 60 A/J mice.
• Using all male mice.
• Mice are the same age (received at 5 weeks
old).

7. Colon Cancer Background
• More than 50, 000 people die from colon
cancer each year
• More African Americans have colon cancer
than any other race
• More men have colon cancer than women

8. Colon Cancer Symptoms
• Diarrhea or constipation
• Feeling that the bowel has not emptied completely
after a bowel movement
• Abdominal discomfort such as gas, bloating, and
cramps
• Rectal bleeding
• Unexplained weight loss
• Unexplained iron-deficiency anemia (low red blood
cell count)
• Weakness and fatigue.

9. Colon Cancer Treatments
• Surgery-for early stage
• Chemotherapy-to kill remaining cancer cells
• Radiation therapy-combined with
chemotherapy

10. Procedure
• Injected mice with azoxymethane (AOM) or
Saline for 4 weeks.
• AOM: chemical used to induce cancer upon
the mice.
• Does not affect the mice instantly.
• Saline: Control injection

11. Procedure
• Mice were divided up into 4 equal groups with
10 AOM injected mice and 5 saline injected
mice per group.
• 15 mice per diet.
• Make and feed the mice 4 different diets
(Control Starch, HA7, OSA, and RS5) for 10
weeks.
• Each has a different resistant content (some are
more resistant than others).

12. Procedure
• Observe and record the changes in the mice
(loss of fur, barely moving, weight loss or
gain, etc.)

13. Procedure
• Euthanize the mice with carbon dioxide.
• Colons extracted and cut open.
• Stain colons to find ACF.
• Determine which diet is the most resistant
(mice with less ACF is most resistant).

14. Results

15. Results

16. Conclusion
• The treatments did not significantly affect the
bodyweights.
• RS5 and OSA’s resistant contents could
potentially be too high that they made the effects
of the AOM worse.
• The HA7 diet could prevent colon cancer more
than any of the other four diets.
• In conclusion, the HA7 diet would be the diet to
choose if someone was trying to inhibit colon
cancer.

17. Acknowledgements
• We would like to thank our mentors Dr. Diane
Birt, Bridget Nelson, and Nicole Cray from the
Department of Food Science and Human
Nutrition for their guidance and supervision
this summer during our internship experience
here at Iowa State University. We would also
like to thank the GWC staff for providing us
with this wonderful experience.

18. Questions?

19. References
1. Angelle, Amber. "Colon Cancer: Causes, Symptoms and
Treatments." LiveScience.com. TechMedia Network, 2013.
Web. 16 July 2013.
2. "What Are the Key Statistics about Colorectal Cancer?" What
Are the Key Statistics about Colorectal Cancer? American
Cancer Society, 17 Jan. 2013. Web. 16 July 2013.
3. Staff, Mayo Clinic. "Definition." Mayo Clinic. Mayo Foundation
for Medical Education and Research, 13 Aug. 2011. Web.
16 July 2013.
4. Hawk, Ernest T. "Colon Cancer Screening Methods." Iowa State
University E-Library. NIH Senior Health, 2009. Web. 16 July
2013.
5. "Colon Cancer Treatment." National Cancer Institute. National
Institutes of Health, 16 May 2013. Web. 16 July 2013.