1. Curriculum Vitae
Jonathan V. Pelt
Jonathan.Pelt@freshfromflorida.com
EDUCATION
• University of Florida, Gainesville, FL—Bachelor of Science, Chemistry, 2001
• Chipola Junior College, Marianna FL—Associate of Arts degree (Engineering), 1999
PROFESSIONAL EXPERIENCE
Enironmental Specialist III May 24, 2013 – Current
Florida Department of Agriculture and Consumers Services (DOACS), Food Safey Laboratory
• Performed all duties within ISO 17025 Accreditation
• Analyzed food samples for Lead residue to determine if present and at what levels.
• Analyzed food and drink samples for Arsenic residue to determine if present and at what levels.
• Analyzed food samples for Sulfites if present and at what levels.
• Analyzed food samples for adulteration of meat with other meats.
• Analyzed food samples for presence of Cyanide as a part of FERN needs.
• Helped to maintain the Proficiency Testing Program that provides tests for the large variety of
methods performed in the laboratory. Also had to maintain list of who needed what tests so as to
fulfill both the ISO17025 and A2LA requirements.
• Maintained the Artel Pipetting Program. Each person in the laboratory has to demonstrate
competence in pipetting annually. This is performed by everyone being involved in the regular
recalibration of the large number of pipettes throughout the laboratory.
• Trained in the Paradigm3 software in depth so as to be able to help others in the event an
administrator was not present to assist them.
• Used or became competent in the use of ELISA, bioplate reader, ICP-MS, and ICP-OES.
• Assisted in the maintenance of the Inventory and Maintenance programs.
• Performed backup of instrument files to the isolated network.
Chemist III September 20, 2007 – May 23, 2013
Florida Department of Agriculture and Consumers Services (DOACS), Chemical Residue Laboratory
State Program
• Perform all duties within ISO 17025 Accreditation
• Analyze food samples for pesticide residue to determine if properly used or applied to commodities
• Use the following mass spectrometers ABSciex 5500, ABSciex 4000, Thermo Exactive, Thermo
Exactive Plus, Waters QuatroPriemier, and Thermo Vantage
• Perform basic instrument maintenance
• Quality Assurance (QA) and Quality Control (QC) of lab wide data
• Develop and validate extraction and analysis techniques
• Use of Quecher and Quecher-like techniques
• Perform regulatory work using EPA guidelines to determine violations and proper use of pesticides
• Use of 40 CFR 180 for a variety of purposes
• Train new analysts in use of instrumentation
PDP Program
• Perform all duties within ISO 17025 Accreditation
• Analyze food samples for pesticide residues to assist federal government in long term exposure
programs

2. • Use Gas Chromatography (GC) and with a variety of detectors including Flame Ionization Detector
(FID), Electron Capture Detector ECD, Nitrogen Phosphorous Detector (NPD), and Mass
Spectrometry
• QA/QC batch data for section personnel
• Train new analysts in use of instrumentation
BP Oil Spill Program
• Prepare standards from neats as well as the preparation of all reagents that need special attention (use
of blast furnace to purify powders, preparing solutions, etc.) for NOAA method
• Assist in extraction of samples in NOAA method
• Extract and analyze samples in Quecher’s like procedure
• Receive and process samples both for audit trail and to prepare for sample analysis
FERN Program
• Analyze food samples for pesticide residues and chemical terrorism agents
• Use a variety of Liquid Chromatography (LC) systems primarily Waters and Agilent
• Use the following mass spectrometers ABSciex 5500, ABSciex 4000, Thermo Exactive, Thermo
Exactive Plus, Waters QuatroPriemier, Thermo Vantage, and Agilent Fluorescent Detector
• Use of Gas Chromatography Mass Spec (GCMS) system for the presence of unknowns (Agilent)
• Preparation of standards for both quantitative and qualitative situations as well as for interlaboratory
comparison
• Develop and validate extraction and analysis techniques
• Use of Quecher and Quecher’s like techniques
• Perform Immuno Assays for a variety of analytes
Chemist II June 30, 2006 – September 20, 2007
Florida Department of Agriculture and Consumers Services (DOACS), Chemical Residue Laboratory
• Analyzed food samples for the presence of pesticide residues
• Used Nitrogen Phosphorous Detector (NPD), Flame Photometric Detector (FPD), and Halogen
Specific Detector (XSD) with a gas chromatograph
Crime Laboratory Analyst, Toxicology May 31, 2004 – June 29, 2006
Florida Department of Law Enforcement (FDLE), Tallahassee Regional Operating Center (TROC)
• Provided toxicological analysis of blood and urine samples submitted by law enforcement
• Provided analysis of suspected beverages for alcohol content
• Rendered expert witness testimony regarding test results and their meaning in approximately 80
criminal cases
Chemist I March 25, 2002- May 28, 2004
Florida Department of Agriculture and Consumers Services (DOACS), Chemical Residue Laboratory
• Prepared pesticides standards for fellow chemists and external proficiency tests
• Assisted in development and validation of techniques to extract and quantitate pesticides and
antibiotics
• Used Gas Chromatography (GC) and with a variety of detectors including Flame Ionization Detector
(FID), Electron Capture Detector ECD, Nitrogen Phosphorous Detector (NPD)
TRAINING
• Received comprehensive training with FDLE including lab exercises, presentations, reading assigned
materials, discussion with a primary trainer, as well as, other trainers, oral and written tests, an oral
board, and finally supervised casework

3. • Attended and completed “The Robert F Borkenstien course on alcohol and highway safety, testing,
research and litigation” from May 15-20, of 2005
• Received training on GC systems, GC MS systems, and LC systems for troubleshooting instruments
PUBLICATIONS AND PRESENTATIONS
• “Analysis of Pesticides Residues in Fresh Produce Using Buffered Acetonitrile Extraction and
Aminopropyl Cleanup with Gas Chromatography/Triple Quadrupole Mass Spectrometry, Liquid
Chromatography/Triple Quadrupole Mass Spectrometry, Gas Chromatography/Ion Trap Detector
Mass Spectrometry, and GC with a Halogen-Specific Detector” Journal of AOAC International, Vol
94, Number 3, May-June 2011, pp 931-941.Amy Brown, Jo Marie Cook, Walter Hammack, Jason
Stepp, Jonathan Pelt and Ghislain Gerard
• “ “For the Love of Bees” a Triple Take A QuEChERS Approach utilizing Both GC and HPLC Triple
Quadrupole Technologies” Presented at FPRW meeting in St. Petersburg July Fl, 2011 by Jonathan
Pelt and Amy Brown
• “Polycyclic Aromatic Hydrocarbon Analysis: A Look at the Fluorescence Technique as well as the
NOAA Method” Presented at the SS AOAC meeting in Atlanta, Ga, Apr 2011 by Jonathan V. Pelt
• FPRW meeting St Petersburg, July 2010 Rapid Multi-Residue Analysis of Pesticides in Honey using
a
Buffered Acetonitrile Extraction and Dispersive Clean-Up Jonathan V Pelt
PERMIT
• Permit to Conduct Blood Alcohol Analysis, Alcohol Testing Program, Florida Department of Law
Enforcement, December 23, 2004 – May 28, 2006