Assume you have isolated S- epidermidis from your skin- Design an expe.docx

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Assume you have isolated S. epidermidis from your skin. Design an experiment on how would you determine whether it is penicillin and erythromycin resistant? (Make sure to include a hypothesis and how you would design the experiment. Also include proper controls and discuss briefly the techniques you would use). (5 points) .

Assume you have isolated S. epidermidis from your skin. Design an experiment on how would
you determine whether it is penicillin and erythromycin resistant? (Make sure to include a
hypothesis and how you would design the experiment. Also include proper controls and discuss
briefly the techniques you would use). (5 points)

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Based on this information, and the binding kinetics illustrated in this chart , would the use of HbF as a potential therapy in this patient be successful? Case 3: A 15-year old girl with cystic fibrosis is rushed to the hospital for an acute respiratory infection and suspected pneumonia. The admission chest x -ray showed extensive bronchiectasis and persistent complete atelectasis in the lower left lobe. Despite aggressive therapy including IV antibiotics, the patient's pulmonary function remained poor and level of consciousness was diminished with no verbal responses but eyes open to voice. Sputum samples were collected via airway suctioning, arterial blood gasses were draw and pH was determined to be 7.32 (normal pH: 7.4 ). As a radical therapy for this patient an infusion of fetal hemoglobin is suggested to potentially increase oxygen perfusion. Fetal hemoglobin (HbF) has different oxygen binding kinetics when compared to the oxygen binding displayed by adult hemoglobin (illustrated below). This difference is in part due to the inability of HbF to interact with 2,3 bisphosphoglycerate ( 2 , 3 BPG ) . Based on this information would the use of HbF as a potential therapy in this patient be successful? .

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Based on the information presented in lecture and your text, in which eon and era of earth's history would the following have first appeared? shark lizard mammal Animals are multicelluar eukaryotes. Al eukaryotes posses mitochondria which they use for aerobic respiration but animals are further distinguished as having some form of internal digestive cavity as well as embryos. Cnidarians, which include such organisms as medusas and anemones, represent some of the most ancient. animals. The have simple body plans characterized by radial symmetry and only two distinct types of tissue ectoderm and endoderm (- diploblastic body plan). More complex animals demonstrate bilateral symmetry and three distinct types of tissues - eco, endo, and mesoderm (= triploblastic body plan). Flatworms such as the planarian Dugesia represent some of the simplest of these triploblastic bilaterally symmetrical'organisms. More complex bilaterally symmetrical triploblastic organisms, such as squid. have internal fluid filled cavities lined with mesoderm ( c coloems) which are lacking in flatworms. Vertebrates are bilaterally symmetrical, triploblastic and coclomate animals which have a backbone. The simplest examples of vertebrates are fish such as the great white shark. Amphibians (such as salamanders) are vertebrates that have walking legs (= tetrapod) and breath air with lungs. While they can live out of water, they must lay there eggs in water. More recent vertebrates such as reptiles and mammals, have an amniotic egg which has a fluid filled sac protected by a shell and this had freed them of the need to lay their eggs in water. Reptiles (which include both lizards and birds) possess keratinous scales and birds in addition have feathers which they use for both insulation and flight. Mammals such as the shrew, have fur instead of keratinous scales and in addition they have mammary glands which they use to nurse their young after birth. The figures below summarize these characteristics that are found in examples of all these group5. .

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At the beginning of the year, Mitchum Enterprises allows for estimated uncollectible accounts of $15,000. By the end of the year, actual bad debts total $17,000. Determine the financial statement effects of writing off an uncollectible account. (Amounts to be deducted should be entered with minus sign.) I am trying to find the account receivable and it will not let me put it 17,000. I am not sure what it is. .

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Background: Many diseases have been correlated with mitochondrial decline (inability to work) including Alzheimer's, heart disease, and Parkinson's. Many of these diseases can be attributed lack of hydrogen chemical gradients in the electron transport chain. Question: Using your knowledge of cellular respiration, come up with a way of increasing the number of hydrogens and electrons in the electron transport chain. (focus on where these hydrogens/electrons are produced/stored and think of ways of increasing those molecules. Injecting hydrogens or electrons is dangerous and will lead to instant death.) .

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Bad actors seeking to create computer viruses primarily must know how to Multiple Choice program code. set up messages to carry Trojan horses. understand network components. bypass operating system guardrails. Which of the following is a goal of confidentiality as defined by the CIA triad? Multiple Choice making sure the right people have access to secure information determining who has access to essential data classifying information as essential or unessential preventing information from being readily available How does each additional block added to the blockchain strengthen the integrity of the entire chain? Multiple Choice More blocks make it harder for hackers to access the network There is no added integrity by adding additional blocks; a blockchain is as strong at its inception as it will ever be. The random generation of unique identifiers that are associated with each block make it difficult and time consuming for hackers to identify. Because each block contains information on recording time and sequence for both its transaction and the transaction. immediotely preceding it, with more blocks there is more transaction history information, making it difficult to folsify, .

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Background/motivation: This Market Discussion will complement some of the key important topics that we have touched on in Lectures: bid-rent and bid-price gradients, how commuting costs shape urban form, and recent market trends for various property types, including office and multifamily. The focus of this market discussion is on (1) the immediate impact of COVID-19 induced workfrom-home (WFH) policies on real estate, both commercial and residential, and (2) the likely long-term impacts of continued WFH policies on CBD / urban commercial real estate markets. The WFH policies that firms implemented in spring and summer of 2020 generated an immediate shock to the demand for real estate. However, the sign of the shock (positive vs negative) depended on the type and location of properties. Broadly, there are three aspects of demand changes to consider: (1) within city reallocation from high to low density, (2) across city reallocation, and (3) variation based on property type. - Within city shifts. The demand for residential real estate outside CBD / urban areas increased dramatically. This was the result not only of workers migrating from downtown apartments/condos into more suburban areas, but also a result of the significant monetary policy shift that drove interest rates down and decreased the cost of financing via lower mortgage rates. - Across city shifts. The demand for real estate in lower cost and less densely populated cities increased as workers temporarily (and permanently) moved to these areas. - Across property types. The demand for certain commercial properties (office, hospitality, some retail and multifamily) and residential real estate in CBD / urban areas decreased. This was the result of uncertainty about whether WFH policies would be transient or permanent, and thus uncertainty about whether work would remain physically located in downtown areas. Despite an immediate decrease in demand for many commercial property types, it is now clear that this shock may be temporary, particularly for the commercial property types that were most affected initially. A particular property type of interest is office properties, given that leases for these types of properties are extremely sensitive to WFH dynamics. 1. Donut effect a. What is the donut effect? b. What can the difference between real estate demand reallocation within vs across cities tell us about how firms and workers view the permanence of WFH policies? c. How can firm and worker reallocation trends across cities be measured? 2. Bid-rent functions, rent gradients and price gradients a. Define the rent and price gradients. b. How did the rent gradient in major cities change from two years prior to the pandemic through December 2020 ? What does this change imply about how much workers are will to pay to live close to work? How did the price gradient change in those same areas over the same time period? c. What can we learn from the difference in bid-rent and bid-price gradients.

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Atoms are composed of a tiny nucleus and a larger surrounding electron cloud. The eloctrons have a negative charge and the nucleus has a positive chagge. Because opposite electrical charges attract, the atom is held together. The nucleus is composed of positive charged protons and neutnl particles called neutrons. If an atom loses or gains an electron, it becomes an ion The atomic number of enthon is 6 . This is also the number of positive p The atomic weight of a nucleus is the sum of the number of protons and the number of neutrons. For carbon-12 (C-12), the atomic weight is How many neutrons are in C-12? All carbon nuclel have the same number of positively charged P have different number: - These different carbon isotopes may eiperiments. The last one is important for madioactive dating Bonding between atoms comes in 3 main forms: 1. bonding involves charge transfer 2. Motallic bending involves shared electrons between 2 atoms. 3. Metallic bonding involves electrons shared with a whole crystal. Salt crystals, also called h ch and are a good eximple of i involve the 2 elements s . bec in shape. bonding. Salt crystals tend to Minerals: use your minerals box and enclosed booklet to learn some basic minerals. It is pretty self-explanatory. Make sure at the end of the day that the minerals go back into the bags they came out of. Cleavage bag You should find 6 samples: mica, hallite, calcite, feldspar, obsidian, and serpentine. Examine the objects, list their names and deseribe their cleavage or fracture. 4 have cleavage, 2 will instead fracture. Does it eleave smoothly on 1 plane? 2? 3 ? If it frectures, is it eonchold on onneven? Tenacity deseribes how well a mineral bolds together. You should find 4 samples: copper, mice, sypsum and sulfur. From the tenacity bag, name each mineral, and give its tenacity, picking from the four choices below: malleable: The mineral may be pounded out into thin sheets. Metallic-bonded minerals are usually malleable. fieriblet Can be bent a little without breaking. seetiles May be cut smoothly with a knife. brittle: The mineral breaks or powders easily. Most ionic-bonded minenls are britlle. \begin{tabular}{|l|l|} \hline Name & Tenacity \\ \hline & \\ \hline & \\ \hline & \\ \hline \end{tabular} Streak. Open your streak bag. You should find 5 samples: jasper, hematite, tale, pyrite, and graphite. For each sample, write down its color, streak it on the provided porcelain plate, record its streak color and its luster (the way it reflects light). Pick the luster from: metallic, sub- manual- open it to the mineral chapter for more identification clues. .

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