A clean water, without harmful substances, rich in minerals, slightly alkaline and that protects your cells and your body from free radicals. fight free radicals is essential to limit oxidative stress and reduce the risk of associated diseases. Antioxidant intake is a way of fighting them. Since 1997, many research programs have demonstrated the effectiveness of alkaline antioxidant water to limit it.

1. Presented by Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology
By choosing to drink alkaline water,
you help your body find its balance.
ALKALINE
DRINKING
WATER

2. 02
To be well hydrated is essential !
Alkaline drinking water
Free radicals / Oxidative stress.
Molecular hydrogen, an ideal antioxidant !
Presentation
Guide
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

3. WATER,THE MOST
ABUNDANT COMPOUND
OF THE HUMAN BODY
60%*
OF WATER
IN MANY ORGANS
+ DE 70%
INTRACELLULAR WATER
/
EXTRACELLULARE WATER
65% / 35%
AFTER INGESTION
IN PLASMA CELLS AND
BLOOD CELLS .
5 MIN
03
WATER,
IN HUMAN BODY
*for a man of 70 kg - 42 liters
Armstrong 2005 ; Marieb et Hoehn 2007
Peronnet et al.2012
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

4. 04
OMNIPRESENT IN THE
HUMAN BODY, WATER
PARTICIPATES IN MANY
ESSENTIAL AND VITAL
FUNCTIONS.
Regulation of cellular
volume
Transport of nutrients
Elimination of waste
Regulation of the
temperature of the body
Digestion
Energy
Neurotrans-
mettor
production
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

5. 1500 ml
750 ml
skin and lungs (30%)
urine (60%)
200 ml
faeces (8%)
50 ml
sweat (2%)
500 ml
minimum
Variabtions
ccording to
circumstances
EFSA 2010
LOST WATER BY DAY
2,5 LITERS
05
TOTAL WATERE BY DAY
2,5 LITERSTO COMPENSATE
NECESSARELY
to prevent dehydration and
ensure optimal cell
function.
Water and drinks
20 à 30 % water in food
70 à 80 %
300 ml Porduces water
by human body
EFSA 2010
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

6. DEHYDRATATION
EVEN SLIGHT
RESULTS
06
HEALTH DISORDERS
constipation, coronary heart disease, heart attack,
stroke, gallstones, dental disease, headache,
fatigue...
COGNITIVE DISORDERS
decrease in memory, concentration, vigilance, ...
TROUBLES PHYSIQUES
rise in temperature, rise in heart rate
Performance drop of 20% for water loss of 2%, and
55% for water loss of 4%.
Manz F. et al, 2003, F. Manz 2007
Manz F. et al, 2003, F. Manz 2007
RJ Maugham 2003
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

7. TO DRINK
WATER IS ESSENTIAL!
TO DRINK
QUALITY WATER IS ESSENTIAL!
07
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

8. A CLEAN WATER, WITHOUT
HARMFUL SUBSTANCES, RICH
IN MINERALS,
SLIGHTLY ALKALINE AND WITH
A STRONG REDUCING POWER. "
SAID BY DOCTOR SHINYA
IN HIS BOOK
The Shinya diet - the diet of the future that will prevent the 3 major diseases
called "civilization": cancer, degenerative diseases, cariovascular diseases.
08
" THE GOOD
WATER
TO DRINK .....
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

9. PRODUCED BY A MACHINE
THAT IONIZES TAP WATER BY
ELECTROLYSIS
ALKALINE,
RICH MINERAL AND
HEALTHY TO DRINK
RICH IN MOLECULAR
HYDROGEN - KNOWN TO BE
ANTIOXIDANT
Water a high reducing potential.
CALLED TOO
Electrolysed and alkaline water -
Kangen Water, ...Reduced
electrolysed water (REW) or
alkaline electrolyzed water - used
by scientists.
Mon K..1997 Shirahata , S et al.1997
Shirahata , S et al.1997 2012
Nishimoto, 2009
Shirahata , S et al.1997
EAU ALCALINE IONISÉE
Hélène DOMINGUEZ
Maître de Conférences
Doctorat en Microbiologie et Biotechnologies
09

10. 10
ALKALINE DRINKING
WATER PROTECTS
CELLS
TO FREE RADICALS.
REVEALED SCIENTIFICALLY IN
1997 BY S. SHIRAHATA
ALKALINE
DRINKING
WATER
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

11. FREE
RADICALS
Reactive oxygen derivatives, which lack an electron,
which makes them very unstable.
To stabilize, they react very easily with other
molecules leading to a series of chain oxidation
reactions.
Generated by the body simply by breathing or
exposed to different physicochemical or
pathological conditions.
Hydroxyle radical (HO•)
Superoxyde radical (OO•)
Peroxyde radicals (ROO.)
Hydrogen peroxide (H2O2)
11
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

12. 12
REQUIRED
AT LOW LEVELS
essential to fight infections and
in the regulation of autophagy
and apoptosis.
KATERYNA KON / SCIENCE PHOTO LIBRA / KKO / SCIENCE PHOTO LIBRARY / AFP
West et al., 2011, Zhou et al., 2011
Li et al., 2012, Garg et al., 2013
Mates et al., 2012
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

13. 13
VERY DANGEROUS
AT
HIGH LEVELS
causing multiple irreversible
cellular damages by reacting with
many molecular species (lipids,
proteins, and DNA).
Taub J., 1999, Lee C.K. 1999, Migliaccio E., 1999, Melov S. 2000
Andersen, 2004, Bagul and Banerjee, 2013, El Assar et al., 2013,
Westerblad & Allen, 2011, Kim and Byzova, 2014
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology
Alimentation trop riche en
sucres et graisses
Consommation excessive
d'alcool
Tabagisme
Surexposition aux
ultraviolets
Pesticides, médicaments,
drogues, produits chimiques
Pollution
Liu et al., 1996, Agarwal,2005,
Harma et al., 2006, Tanriverdi et al., 2006,
DIFFERENT FACTORS PRODUCING FREE RADICALS IN
THE HUMAN BODY

14. 14
A BALANCE BETWEEN FREE RADICALS AND ANTIOXIDANTS IS
NEEDEDGOOD PHYSIOLOGICAL FUNCTIONING.Peng et al. 2014
NATURAL DEFENSE
SYSTEMSFROM THE
BODY
such as antioxydant enzyme :
superoxide dismutase (SOD)
catalase (CAT)
glutathione peroxidase (GPX), etc..
Glutathion Peroxydase
(Antioxydant Enzyme))
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

15. 15
EYES
Macular degeneration,
Degeneration of the retina
HEART
Cardiovascular illnesses
Ischemia of the myocardium
BLOOD
Hypertension,
Arthérosclerosis
DIFFERENT
ORGANS
Aging, Diabete, Chronic
fatigue, fibromyalgia
SKIN
Aging, Melanoma,
Psoriasis
KIDNEYS
Renal insufficiency,
Nephritis
IMMUNE
SYSTEM
Autoimmune Reactions,
Inflammation, Lupus
ARTICULATION
S
Rheumatoid arthritis,
Classical arthritis
LUNGS
Asthma, Allergies,
Cancer
BRAIN
Alzheimer, Parkinson,
Embolism, Cancer,
Autism Taub J., 1999, Lee C.K. 1999, Migliaccio E., 1999, Melov S. 2000
Andersen, 2004, Bagul and Banerjee, 2013, El Assar et al., 2013
Westerblad & Allen, 2011, Kim and Byzova, 2014
ANY IMBALANCE, IN
FAVOR OF FREE
RADICALS, LEADS TO
A STATE OF
OXIDATIVE STRESS
causing aging and pathologies
AGING AND RELATED DISEASES AT A
STATE OF OXIDATIVE STRESS
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

16. 16
Hélène DOMINGUEZ
Maître de Conférences
Doctorat en Microbiologie et Biotechnologies
FIGHT FREE RADICALS
IS ESSENTIAL
to limit oxidative stress and
reduce the risk of associated
diseases.

17. 17
ANTIOXIDANT INTAKE
IS A WAY OF FIGHTING
THEM .
Since 1997, many research
programs have demonstrated
the effectiveness of alkaline
antioxidant water to limit it.
Liu et al. 1996, Komatsu T et al. 2002, Chan et al. 2002, , Li, Y et al., 2002, Shirahata S 2002, DAN J. et al. 2006,
Ye, J 2008Fujita K., et al. 2009, Li Y. et al. 2011,Yan, H. 2011; Spulber, S. 2011, T.Ishibashi et al 2012, Shirahata et
al. 2012 He, B., et al. 2013, Qian L, et al. 2013, Tomofuji, T., et al. 2014, S. Ohta, 2014, Ichihara et al. 2015
1.000 ARTICLES
1.600 RESEARCHERS
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

18. 18
DEMONSTRATED EFFICACY OF IONIZED ALKALINE ANTIOXIDANT WATER BY NUMEROUS STUDIES
ON MANY DISEASES AND ON AGING.
Publishied by Shirahata et al. 2012
IONIZEDALKALINEWATER
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

19. PROTEC THE CELLS
BY REDUCING
OXIDATIVE STRESS.
Professor S. OHTA
Nature medecine - 2007
MOLECULAR
HYDROGEN
19
EFFICACY OF MOLECULAR HYDROGEN DEMONSTRATED BY CLINICAL TESTS
ON SEVERAL COMMON DISEASES.
ALZHEIMER
ILLNESS
CEREBRO-VASCULAR
DISEASES
INFLAMMATIONS
RHUMATOID
ARTHRITIS
DIABETE
HYPERTENSION
OBESITY
ARTHÉROCLEROSIS
CARDIOVASCULAR
ILLNESSES
CANCER
THERAPY
S. Ohat. 2014
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

20. AN IDEAL ANTIOXIDANT
20
Penetrates inside cells and cell
organelles.
DIFFUSE QUICKLY
even at high concentrations..
NON TOXIC
The most unstable, reactive
and toxic species derived from
oxygen, against which no
natural antioxidant is produced
by the body.
NEUTRALIZES
THE RADICAL
HYDROXYL (OH•)
Fontanari et al., 2000
Ohsawa, I., et al.,2007
Ohsawa, I., et al.,2007
MOLECULARHYDRGEN
for example, drinking ionized
alkaline water that naturally
contains it.
EASY TO ADMINISTER
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

21. WHEN THIS MACHINE TRANSFORM
YOUR ORDINARY TAP WATER INTO
HEALTHY, FRESH TASTING
ALKALINE DRINKING WATER
Dr. William KELLY - author of the book Healing Cancer "Alkaline water produced by an
ionizer, has become the most important progress in health care".
MOLECULAR
HYDROGEN
NATURALLY PRODUCES
21
Hélène DOMINGUEZ
Professor University
PhD in Microbiology & Biotechnology

22. CHANGE YOUR
WATER !
DRINK ALKALINE
HYDROGEN WATER!
ADDRESS MAIL
dominguez.helene@gmail.com
PHONE & WHAT'S APP
+ 33 6 98 89 39 53
22

23. 23
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Doctorat en Microbiologie et Biotechnologies

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RÉFÉRENCESBIBLIOGRAPHIQUE
Hélène DOMINGUEZ
Maître de Conférences
Doctorat en Microbiologie et Biotechnologies