Hao To has over 3 years of experience in industrial research and academic laboratories. He has a B.S. in Biochemistry and Molecular Biology from UC Davis. At his current position at Adverum Biotechnology, he constructs and screens AAV capsid mutant libraries, optimizes recombinant plasmids and workflows, and performs techniques such as molecular cloning, cell culture, PCR, and ELISA. Previously, he assisted with research in organic chemistry and microbiology labs at UC Davis.

1. HAO TO, B.S.
212 CheckersDr.San Jose,CA 95116 ● (408) 876-0350 ● haovinhto@gmail.com
Education
B.S. 2010 -2014 Biochemistryand MolecularBiology
College of Biology
Universityof California,Davis
Key Qualifications andAttributes
 3 yearsof industrial experience
 Recordprotocols,procedures,anddatainnotebookregularly,detailingexperimentalpurpose/design/results,
on a consistentandtimelybasis
 Optimize procedures forexperimentalandtechnical problemsusingbothliterature andcolleaguesas
resources
 Organize, analyze andcommunicate dataeffectivelywith teammembers
 Work inteamsto achieve changingprojectgoalsandmeettimeline
 Act as technical resourceswithingroupandtrainothersas needed
 Conductall research-relatedactivitiesundercompliance withGXPapprovedprotocolsandabilitytomulti-
tasks
 Learn eagerlyandapplya large repertoire of benchskillswhilegaininghands-onexperience inafast-paced
laboratory
 Receive verbal instructionsandworkindependently,mostly unsupervised
 Strongwork ethic,self-motivated,highlydependable,positive attitude
Skills and Techniques
Molecularcloning Bacterial & Cell Cultures DNA Sequence Analysis
AAV & BaculoVector Transformation,Transfection,Transduction PCR.RT-PCR,qPCR
AAV Production Mutant Library Construction FACS/ FlowJo
DNA/RNA Extraction WesternBlotAssay, Endotoxin,TCID50, ELISA Gel Electrophoresis
Work Experience
Research Associate (I – II) July 2014 – Present
Adverum Biotechnology, Menlo Park CA
 Constructed and screened rounds of multiple mutant AAV capsid libraries through direct evolution
 Determined the double integration rate of a mammalian integrase in cells at various donor plasmid
concentration of GFP & RFP through transfection and selection
 Optimized recombinant plasmids to increase AAV library production

2.  Optimized and streamlined workflow of high throughput molecular cloning, mini/maxi prep bacterial
culture, test for gene/protein expression, and sequence verified
 Designed and cloned plasmid, familiar with AAV vector and Baculovirus
 Maintained and generated stable cell lines: HEK293, HELA, CHO, RPEs and run FACS if needed
 Performed standards assays: qPCR, WesternBlot, SilverStain, Endotoxin,TCID50,ELISA
 Managed in-house plasmid core data base and released constructs to cross-functional group
 Trained lab members who needs assistance on cloning strategies and process
 Presented weekly reports in group meeting
 Organized the lab and managed purchase orders
Research Laboratory Assistant February 2013 – July 2014
Toney’s Lab, Chemistry, UC Davis
 Developed and optimized pre-steady state kinetic study of Horseradish Peroxidase (HRP) using
stopped-flow
 Designed and conducted experiments on mutagenesis of K258A Aminotransferase in PLP Derivative.
 Successfully assisted graduate student with his research to identify one of many PLP derivatives and
its pathway
 Exposed skills include: NMR, mass spec. TLC assay, affinity/size chromatography, and general organic
chemistry techniques
 Critically analyzed results both independently and presented them to group meetings
Lab Teaching Assistant September 2013 – June 2014
Microbiology 101,UC Davis
 Assisted Professor during lab sessions, including: lab lectures, lab demonstrations, supervised
students on their works, and distributed experimental materials
 Instructed students with their weekly lab lessons, reviewed their assignments, and answered any
lecture & lab questions
 Maintained lab safety and hygienic environment
Reference
 References can be provided upon request