Discussion session hosted by Leonie van Drooge at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Document Data Modelling with Couchbase Server 4.0Cihan Biyikoglu
Couchbase Server 4.0 is both a document and a key-value data store. In this talk, I detail the steps for designing an efficient data model for Couchbase Server with examples that demonstrate when referencing vs embedding is the right choice. I also walk through how N1QL, the new SQL-like query language for JSON data, makes it easy to reshape the data into the format your application excepts without friction.
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Document Data Modelling with Couchbase Server 4.0Cihan Biyikoglu
Couchbase Server 4.0 is both a document and a key-value data store. In this talk, I detail the steps for designing an efficient data model for Couchbase Server with examples that demonstrate when referencing vs embedding is the right choice. I also walk through how N1QL, the new SQL-like query language for JSON data, makes it easy to reshape the data into the format your application excepts without friction.
Website development company in delhi ncrCss Founder
Website Development Company is fastest growing company in the IT market for the website design and development. we are best website development company in India as well as in USA we are based in Noida and Delhi NCR. Website development company is powered by Css Founder.com
Discussion session hosted by Leonie van Drooge at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
The in-vitro approach: Qualitative methodology to explore panel based peer re...Gemma Derrick
Presentation given by Gemma Derrick and Gabby Samuel at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Multiplying method: Ethnography and the reconceptualization of evaluation stu...Gemma Derrick
Discussion session hosted by Pau Wouters and Sarah de Rijcke at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Focus! A discussion about the use of focus groups as a methodGemma Derrick
Discussion session hosted by Leonie van Drooge at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Rethinking the 'international' in the governance of scienceGemma Derrick
Presentation given by Tereza Stockelova and Sarah de Rijcke at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Intentions and strategies for evaluating the societal impact of research: Ins...Gemma Derrick
This research in progress paper describes the initial results of a long-term, large-scale analysis
of the operationalization of evaluation of the societal impact of research. Results from the
first stage of qualitative interviews are used to illustrate the strength of the methodological
design of the study.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Discussion session hosted by Leonie van Drooge at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
The in-vitro approach: Qualitative methodology to explore panel based peer re...Gemma Derrick
Presentation given by Gemma Derrick and Gabby Samuel at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Multiplying method: Ethnography and the reconceptualization of evaluation stu...Gemma Derrick
Discussion session hosted by Pau Wouters and Sarah de Rijcke at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Focus! A discussion about the use of focus groups as a methodGemma Derrick
Discussion session hosted by Leonie van Drooge at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Rethinking the 'international' in the governance of scienceGemma Derrick
Presentation given by Tereza Stockelova and Sarah de Rijcke at the Workshop exploring Qualitative and Mixed Methods in Research Evaluation and Policy 2015 (QMM2015)
Intentions and strategies for evaluating the societal impact of research: Ins...Gemma Derrick
This research in progress paper describes the initial results of a long-term, large-scale analysis
of the operationalization of evaluation of the societal impact of research. Results from the
first stage of qualitative interviews are used to illustrate the strength of the methodological
design of the study.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
FAIRSpectra - Towards a common data file format for SIMS imagesAlex Henderson
Presentation from the 101st IUVSTA Workshop on High performance SIMS instrumentation and machine learning / artificial intelligence methods for complex data.
This presentation describes the issues relating to storing and sharing data from Secondary Ion Mass Spectrometry experiments, and some potential solutions.