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PARUL UNIVERSITY
ND EX
Experiment/TBe
s.NO Gate Teachers
Sgpaturs
Marks
Dosby Epthelal and
COnnEve bissuL oE
uman body
2J o study MasulaY and
Nentus bis oF
Aunan b
S-2
3JTnbaducbonbo
Haemogytamey
19-12
Debeminatien oF
uhibebland ellCwl)
DE own blaod
B-
16
S1DeteminabonoF tobal
Kad_bload cell CRB
COunltoF ownlaod
17-2
PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig. Na.: 0
Dale
UNINL
PRACTICA-L
AM
To StulyLpitkalial and Comeckive issue_aE Auman
bau
YREFERENCE
CaOUa Rk _an Dr PatelN.M Practical Anatogy_and
PSialoauL edihan Zoo1-Zalo_BS Sha pakashan
Akmedkid_Pa Na- 99-lag
0-B.S
ShaDakas
KREQUIREM ENTS2
charbsSLies_
PDESCRIAIIaN
SPBalial Tissues
Tho ypes oF cayennq_andhnin4 epitelial hssu are
classried atordingto two aacteristic
armlgemant of Lells into layes al o sh4pe) OF__
Cels
A AragenNnl.ofcelsinto layer'and theshapeEbhe cells.
a Simple epiikalwm
PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig.Na.:
Date
- a SinplepLmItis aSiAgle lauerof cels bhat_
Funchiansin dHisSiA OSMasis,tnan, Seetinn o
ahsopian Secebon is thoPrnductian an leaje af
SubsEanceSALh_ MuK S/eat,OY en2ymes t6104phA
isho iaEakt_0E BHuids_Dr Athor SubstHnces Such 45
dgeste ood renAO intALinal Eact
Sin ple SauoM.auS SbetifFied epVann
Cuboidal
PeudhstakER_Rptbalun AppeAs_to_haVe_multle layes
of CelS Decaue Be relAucle le_at_dFftvent lels
AAd AaE allcels
exten to 4pcal_Suface my contain lia o he
Caahlek cells Selrabs_MuCAS PeldastxabliEzed_ephelnuais
achualy a siMple_epitalu hecase al ilscelk_restan_
tho bakenint_nembin.
reach the opica SurFae_Cells tatdo
Smpe Cuboidal Sbacifi loidalA
Glumal cstaified epatkelum Conssts E tun Or mphe ayes o
Cells that pntect nderlyin_bissuo_in_locatinswhore
Hore 15 LbAsilerable weakan tear
ololo90o|0 BICellshapehe Lell Vayinslape_epalinq_onair
FUAcbion
Sinpe columar peun Starihtd una aSuamausCalls axt ahagt lhte Flanr tiles_a1dak_thn
whilh allaus_o R_teíipalsaqe OF Suhstance
luhadal cel anasLalas Hayak_w andak shapa
ie Cuhe Y horagons y_Mat hale Micoull_alher
Apica SurFace an Functhan etAor Secvetion_ar
dhsarphion
PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg Na.:
Date:
cCalumAAr cellsat much baller han boy are wide lke
CduMAS and pntect underlyin tisSuos Tor apical
Surfaces May_ANO_Cilia nicovill and 'thy ofte
a
Specialiedor secHLion and ahsarption
1 ransLtidna cel Chanae shre on Flat to_cuhadaland
6aaSoAASuch 4sho unuy bladoer sbeck tD
naer Size ad Lan collapse a maltr sizæ
DGecause_of te_diueriy_oF celk_and etracelularmabir
and hediFFerencPs in Beir relativP prapartions the
lossi fiation o COnnertVe tissugs otaluays dlear-ut
and SeVëal claSEcatiin_Pis
i)omecb/e TissusS
-ep
Nuceus
mbyyan Conneckie bssun
A Mesencheme B Mucav Connective bissu
2)MabuH CoAnechue tissue
Rehalar ti
BDenseCnedtive tsu
A) Lonse Conmectwe bsu
Avealar oAhie tSu DDase_reaular su
Fbsikt c
Dene ingauhybstug
Colagen Tg
3 Eashr ctibsu
3)otuhr comedise tsu
Flrelet ee
j , PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig.Na.:
Date:
cioiog DIBonvtösue E Lauidtastcbieba
Filole arulage
auoe Bload tissu
alinCazhlage
2lymph
Fhncaztilage
31lasbc (alage
reylay ConMeivehis
Cpsael
MAOL
Corlge
cs
Flaolye
#Cartlg EoicA
Melb
COmi
RL
Bloo
BonA
PARUL UNIVERSITY
FACULTY OF PHARMACY Ig.Na.-
Date:
PRACTICA
KAME
10 study_MusculaY and Nenhus bisuoE Auman hady
VREFERENCE
aOual Kkald Dr_LatelM.M_Prattiul Anatamyand
PAMsialag y tatian Loni-2alh_B.S Shah Prakashan
Akomuaad Pa no 1-h2_
EREOUIREMETS)
Doarts sldes
LOSRIPIION-2
)Muscuar bissu
askalebalMNcle
Sskeletal muscle SS is Aome For its latatian-itis
Sualattrkad bo the honus OE he skeleban AntHer
Feabu i its Stiationsalternatip lah and dakhand
withnthe Eihes thab are _isihleundter a liaht mcd
Dheletal Mude iCansiered Valultayeause t Canle
Made to conbac or elak bu CiSCiaus CoADal kalaa
MMsdle_Ers_con_ Vay in letatK rom a Feu/Cm_Ln
shartmuscles to uph 30-4b inupur longatMucle.
PARUL UNIVERSTY
FACULTY OF PHARMACY Date
SAIMusdle Fheris raughly culiadrita inShapeand
has maly Aucle lacate at ptrighory ithin
a uhaleMUscle tho ndiuidual usde Ehres may
he Panll0_bo_a0 angther
celauc
Smooth musale
Smanth muscle Lssuois lacatedin_thewallsOE hollo
inLenalS1MELsSuch 2S hlaad /essek airuAys h_
h ungS Stanach, intestines4al-jladder and
U i t a r y a d e l e r
Is ConbncEian hip Consbid or Aayaw He lumen_aFE
Anad vesel Pusially break daun and mk_faad
aloA_ aasbainteabiaal_tat mave Fluids_hiagh
thadsar eimates /astes
SMayh Mede
SMathMusclo_Eh a uSually intalunbay and hau
are on-sbriaked,heace the_bezm SManbh A_SMath Mede
ERY ISSmall, thickest iA W Middle Qd tapentg at
each ead IL onkinsa Sih4le cenbaly locateAclod
p jundtians CanMect Maly indilidual Fhes n SMaath
Musde tssw Far ea nho walL_oE heiAtetnes
Such muscle tissugan_praduce_PoweFul_Contachdn
as Many MusclO Fhes COntaCE in_UAon_n_athez
lncatidns Sugh al [Ko hS0F IKO eye smaath Musclehe
Conbract individually likaSkaletalAcleIAs_Llcase
Aap ulchons ake alieat_
PARUL UNIVERSITY
FACULTY OF PHARMACY Date
CardiA Musale
Canlia Muscloe bsu orms mskaf ble walla£
heheakt Luke skoleal Muscle it iS sbaated
However unlike_SkalebalMusdebissuo tis nvalue
t5 coALrattidn 1S An1 ConSCiauly contralled
Puced
Cariac Ascle_ibres_are_bzanchad and uiually have
LenDnillylacated Aucleus_AN DCtSiana
end to end_by bansveAe
plaima MemhzanCalled nteralabed
isS hith CoAtan_both desmnsames ad hap_junctians
I ce has uwa Audle Aou attah
hick enip4S_dE
Conac Muge
Dhalated dC ake uAiqIu ta Cakdiac Musde_ssw
h deOMOSamOS Stenqtkin h_ss 2nd hld_a
Fbres
7hoap Junctions piuide_a nute For
OF Mulle actidn Potenbial thnughaut L hond
togetho_duiAG teir VjgOraus COAtactians
4uick Condudn
iMenaus hissua
Despibe the awesomo_complexb aFthe Nerloussysten
CoAsists_af only tulo pincigal ap AFcellkueglia.
andNeuHnnS euEaA And ne es a SeMSitive to
Vahausstimul Koy Convert stmuhinto_elechial
Scanals
CiNductheso actidn bteatials to ather "eulani
t Muscde tissues ta_qlands
Caled atton_PDtentials WveiMpase) and
PARUL UNIVERSITY
FACULTY OF PHARMACY Date
MosL NthOnS_CoSLL af rte_basic paslare
badu and wo knds af Cell puesedwne
afons 7ke cell_oy contains the Auleus_omd ottis
O 4 a n e l e
Dendate)_are tagring huahly branchedd UuallySue
CellproLeSs They ard A najor eceula iu Apat
SinglethCylndatal_
Prncess that may e_veyleg
STEisthe Output ition af a utanLolduting
0enimpulses bewar annlher Aeunl or totame
oer tissuO_
yen bough ARULglhia_d Aat pinLnte Av Ccnduct
Nerue iMfyeS 1thase LelS do Ae Maly MLLtnt
Supparti Eunhons_AniM4 Placed hat plate
thd at_plate wWl AdE a 4 NoLAuÍ St/mulahen
PARUL UNIVERSITY
FACULTY OF PHARMACY Dale
PRAC TICAL
Tatreductian to Hemacytometpy
(REQULREMENIS 9
I>HaemoCytometAr St uhithCOASiSLS OF NuhauLyS counta
ChanherBLPptte, Ihonas calirslf Lulhl_ald
MiLastnpe
DREFERENCES
aONal Rkan Ur latelNiM Prattical aAutamy_and
Plustolagy lhedstion, Zoa-2ola BSshah _}akashan-
THEORY
TAo COuAtia/cells_CKBCorWBC) in_Aload usinq
haemocy tome set_is Caled as haemofytomat
Tho umherot_Lls_in te_blooz a_too MaAy aA4
tho S2 OE Cell s too Small Tke_cells w apar
in cluster E Seen_bhznugh_MiLLoxape ThiS difikull
S partlyoVerrome dluting_Lland to knnuA_degret
2 HatmgcyhaunmuttrcoASstof iDluAing Pipettes i)Cauntiua
Chamk (ecial CaVeslp-
PARUL UNIVERsITY
FACULTY OF PHARMACY
Date
ie Diluian Ppats
DIL Cansist_aE Eour_parls-Thelang stemhe hulh,th
Shortstem_and
and the Sucker
The lan4 StemAa a Unulon_capillazy bore tteding
fomawell Around ConiLAl up And meain4 inthe
hualh The long s£eM isdided into lo Ezd Pozts
Fom_tho EY to t malh 1ust neay"tAg bulk
Rulerbde
Bul
Beat
Mgulh
Piece
JteM
The FEth 2wisidn iskeauly _maked and laleledasd.s_
This_pat IS o Mei exact amount o blood
takenForCounting o Fluid iA this_part does Aat
bake Payt in tho2lutiaA a1d eALe this quantty
M _deducted wh.l0_calalating thediuin Fr
s
-CafilbyjoN e bulh is part wlere t_eilubiano blood Lake_pluce
It COnSst oF whte or edhead uhith kolps_in
uAifbm MiriAg Of blaad with_2lution
COAical Ei
Fluid The_
RBC ppetbe WBC pifette ulb2Ads ina_shprt stem which have_the 11ar
lol he Shart stem alo cansist_of_ulu+n
Caploy bore_
Lt hoy red lead I t ha ehte bead
Calilbatian 0-S,1
any
Calatian- 0.5, L0 TAo sucker is_Flbed to tlo_eAd_oF Short stem_7
Sucker 1s nbher or_Palytlong tule Attde end of
huhe bore is toplasic mauth Piece
and lot
Sze of lalb ib lupger Size df bulß is sanadle
Maih fiece is red ntolbur Mach ece is hie inday
8lunon Can be dln upb bu,
Diluti on (an be cone vpto
1D0 or Zoo tmes
PARUL UNIVERSITY
FACULTY OF PHARMACY Dale
Counting_Chamler.
1E i5a Sngle piece thickalass slide hauiA4w
COuniAg-aFta iA_CeAtalpart Iha tuD Cohti4g artas
ave e fakate iam eah ober _part oF SM y
an Hshape araolL_on tho eitbor sije_ai kse
Aendsmal
are platforms uhich are nised y 0.Lmm
area
ahoue to_Cauninqarea Onthee plalfams_zeLes
SPecia Cauersliphamas Coversli
CantalnZ-15
Tho ulin4 areasof Chanlher_are tao shap_CoAtast
Ae laire oF_brighLoes a4ainst thedarker_Metahai hakqmun
ARA
JE 1e A Ai semibranparent laser oE the_melal_h&
heen degsibed pA tho4laK Countingared and tho_
liAes_ar hled pu hraug
USE aS OA COAAat SeeEeStais dunb4 the 2hu
Be ulia Canat be Seen in hnghi L4hEs Focddd
DA thechamher_
hob-0,002SMman o dhal Surfe
Volune ofer a larre Aur
CArea-dert oime
F0.ul
2lonljust the chamheroe_must partinlyclase tke
diaphram_ oF te hicaso2e
0-looomm 2 e takal_aling ared_oY each slide is `nnn length
an 3mnin Headth T isdaided intoAiAe _tqual
SAuars DF 1smm_ared Th bondazs_lines oF Bese
Stuares Ae bnplelinings
0-0025M2
Ne
uaul ste fouS4uarts oF COLneIS Ve used For wBC Counting_azhie
Sfud I) For RBC COUntin
PARUL UNIVERSITY
FACULTY OF PHARMACY
2 equal small KRC s4uares,and lacn al thae
73Smal REL
SmallestSpuAAy Sngle hn4
hus uhale Cenbal Square diyide no hon SMallet
hWluiea flud
VaiauS_lutian Flus a Se n AALmacytam2y
hut te hasic Chtlna or_peparil4 bo_Hbo fu
tat it skaul ke iytani td plam
To compasitianDE dlutial Flui depud oA dtbr
Yeaiteménts such_a StaitiA4 cion e Tahlo7 an
temast_Commanly usedilution Fluis ior KB
4
And uWBLCaunt wth purtalke af each cOnsztun
PARUL UNIVERSITY
FACULTY OF PHARMACY Date
PRACTICALL
SALM
Eauneation of h1be_blood_Cell_Count of 6uA bload.
KREQUIREMENTS
COASshS of NeulauLerls Countog
HALMactometerSet_ ulhizk
chamber WAL pipete Thonas coverslip,uwatch alass, WB
dutina Elud Pickling Aeedle 201:allohal fal an michige
EEEREALE
Goual k _and pr_laklwM Prchical Amtarmyapd
PAsialaay edition,2os1-2olo Bs shah _pritkahan,
Pmedaaa laMa I-3
ETHEOKy
Mumber_aF uhite Bload cells in hlood ae tod may
and e sine_af cedsis too Snal T i Korefore
| Adt gassikle a_caunt tho cells euen under Agh pouk
his dificulty is_jaztialy avercone u dilutitge alaod
ih a_Sultahle_dlutionFluid toa kKoun deare Te diluted
Alond is_placed iA a capllay spale or kndA APaci
etaleen counting chamberd Cove Slp-
Te Auber_of Cels iA thO_SMall_capllas SPACe_oF knvwN_
/nlune is ten Couated uade b ighPouje at
MiCosLoLe Tho CoAt Can_1 Catrulated hymulkplurg
YL PARUL UNIVERSITY
FACULTY OF PHARMACY Dule:
hoAumber wb dlution tactor,DIsuyhance A e
pf Lone ondposure i e Lht sAL o
MaunLanence
LenLellyMedaleySKleLalMude relacat.on
2Compasulion of wBC D/lation fluz.
Glacial ALeicau 20ml 2Desboy kEC
henhan ar MethylValetU/) 10ml2S2uns AudeotwR
Wabey upto Lwm Dluent
PROCE DURE2
e homas coVealip,Lountung_chamber and be_lenses
oE MCnsage are leantd ir ith the_Aip_aylal
2 Tho thamas CouAhagchanher uasacdiusted a oheNd
Ader lou paulr E_IaoPe_keLpA4_ti_thamai
CoVeslP sty 0A toplallarm af slide
2 e ng Finger wa sterized wuth tho alahal and t_
wAS phkedhadly uih tte helpE pritkhag_Aeede
e Fist dnp_o blaod u!as
hp Was SuIked_ia bo WRpipette up to tdoMak
oS inmeJiately _be W/BC_lutián Flui
Mak
disarded a e Secend
a Scke_up
12 pitle_uwas nled bet paln o mir Rlacd.audl ful
m y P A R U L U N I V E R S I T Y
FACULTY OF PHARMACY
Dale:
lew desare distavde and bun pgelte wasd a
a4glß oF 4to
HosurfaLe_af
ounbinr Caner
AA Lpwas Aplied bo be_
NanowSLEJELue 7A2
COMting Chambera be coerslp
>(CALCULA[TO
3 S3
6 3 B
7 The_Eluid was_alauted to settle nr hre niAutes
e sta4e of miosape
32 6
42
8 e WRC damjer uias locate and WBCS,aL COUnted
in Mallest1 sAuaes AS wa epeated iA_anatser
yeeSuch
such_Chamhes
2 7
33
2 3
2RESVLT2
T BC Caun_ AF My_dun 6land uwa aund
Lo le_2.20/mM
A-9 8:77, C-43,
V-3|
wBC: No. of celx DF pd
OISaSS TaNV
Caunted 2SSX 2o
Artax Depth 4xO. GeAeral chaactersties dE WB.C-Leuocye are
wandehng cels whih 2re_Audleated_aA d lot
Contaiany haemaglokin_Tkey a Shghtly_biager in
Sine ad_sn_Aun~ haiongin is erakasala
braAulgyle_ae dened Foled onMarau uh1le
Aaaulatute _are dehuked spleen ao umph4lands
Tee
2SSx So Cmm
l2.7So /Cmm
fe spanistoo shart Tew haus to Feu daus
2Nosal wßC Cautis hgon-_lloco_lcmm IFKe coun goes
belou L-00nlcmm it iS knaun as leucopenia Beydleucacy
PARUL UNIVERSITY
FACULTY OF PHARMACY Date
ynctian_of WBL
Phajoglaus Tt i engulEing cf forign_Mattna-
Backek D foreian_pirbiclE
22Anthady_Famaton.Antihadia a hamma-hloubns
fomein ryanse t invasian Anyanjii
Funchitn is iame autympaddybes
3 Formatian ofFibzellasts wB playsan iMporte role
in_ bssue hepair A inFlanmattun mphsatee
are 4enWrally oñVEed_ntp_tdee_
Ljkhntan_of ALtamiAe_anz All2ak eLtian
£DsinDghil ah ChieFly_iAualv n thie reactians
SSeetiol_at AgAn Hepnhn san anticnaq1lant
Basphs ak 4aWally hih in_Aepahil
V PARUL UNIVERSITY
FACULTY OF PHARMACY Dale
ZPRACLICAL54
DAIM
EAuMerat.idn al tatal RBCault al OW lacd
EQUIREMENLS
Neuhauer CauntiA4 Chamber KBL Pipetbe mai
Caver sp,Watch4lassRBC 2uta4 Fluid Pnklng
APelelol allahal yldl and iLadpE
REFERENCE-
DGayal Kkand Dr latel_MM attdlAnatun
an phySidloqu_l edtin_2o01-20lO. B.Sshah
prakushdn_2Amedalad Pa A4-22-2S
e_Aumber_oF rdcels in lood_ae tan maAyan
S12e_of Lells s_to0 SmalLt iSBoreore_AAE Posszale
t Caunt tho cell euen_UAder hi4h POwe hs
_dfficulby is_partial_avercane_hu luting tae slan
with aS talle_dlybian Eluig a khoun_2eake y
_dluted blod is phted in a_capillansSpatt_EKALUN
Capaci ty A etueen CounM_ChapiheanCClor sl
kaguA Volume is ika (auAteuniep K higa outr
INa1
PARULUNIVERSITY
FACULTY OF PHARMACY Date:
O_aunt can Je calculat y Multgly.lg
Awniber ulith the dlatianfuctar
Suhstande Amaunt Puipe
2 Spdiumchlgnde
2PreLent ncly
Praviie pteniaty
revents ouler oML
2Ssdhum_Suphaior
Mercuric Chlaridle SAn 2CaKe atiinafLell
wAter ptalnm
PROCEDURE
DTe tomas caverslit, Caunbugchamhey and tho ene
of MiCtascage_uQre cleane ikt h the help_
Te thamas CouAtingchamher ue aduitedan_obserd
forKBCsphaNSUAder_lou_Pouer of Micokafg
Keep04_ toIhoma CQUASliP r0tin4ah the Platfakn
a HeKe slhe_
ddiute for Aigh Pau and chamder ofBC Sak au
P A R U L UNIVERSITY
FACULTY OF PHARMACY Dale:
7 KE pipttle / cleaned ao di h M4
wa Styhza wi e_Alconl A1 Y
Ae fust_ap_af_blocdwa 4LAd a TA
SecMd dropwas SuCkin KL pifette upio
imnediatply e RBL 2luLonFlui
was Sucked up taMalbl
heLLHe_was bizughi to a Áon2aAtalzaiti
pipetteAGMple kaht was_41/A tothe ubuer tale
ePiPeLtP_WA led betu/len ThO_Pulm_oMik_
h hlond wthIA_zlutian Fluid for_aN MAuP
2 feydrapS_ave_dscaded_and then_Pipette_was hela
atho aale oE s°tothe surface DF LountiA4
etuen te cputing haMbEY_ an tiO CoVeslp_
2 70 Fuid was aloued to Settle for_thre MiAute on
stage_ MiL1ce.
7e RB humher was located and KBLsAe(ounted
inMalest6 14uare.hS WSy LefeateZ_In_
aAather aur sdch chambes
eMA, PARUL UNIVERSITY
FACULTYOF PHARMACY
TRESLIS
7 LBC COut dE 4_aun Alanz ws faund i
he S09o.aonlca
YDISCOSSIO
B 2 had_carpsdes are ciuar hcancaeLad-Audete
cek Tey ConLan Epnaiony Panint_taenagioial.
Aomal RL3 121n dianeier ad 221 AAe
RBCNo OF celcounbexDilutionacdr
KDepth xea Tg are thaner in centre thuSAfeahjg_kedum leb
Shaped RBL Formein gleen a l h enbp n
Dalts RBL FOMMA in rihone maimu
= Nxloo xloxS
So9 x lo000 DfOmatian RL isknguin as eathngitys
So9ooo0/Cmm RBC shau5 Variusprgorhes-
Haemalusis 1FRBCi5kept in huprtaiz_Salutian
5wells aad Einalynes Eating haEMOahlin
ThISiskopuA_ Haemdluis
KaularFumationL StPngerty_pE KRCtO CDME
Susfindi_1A_pläna os lag asndi fekg_
P A R U L U N I V E R S I T Y
F A C U L T Y O F P H A R M A C Y
Date
ylbagyle Sednentalion pat
TE KBC d_allaved to stand an anb-C24ulatan
Supenata0t
2SR_3_defined_as MMOF t lear lasMA Fameda
ho top ot yeztial ColuM i0 a Aour t isrecnal
oF SuSPenSiOn s tely
2ESR-_tmm/hauy ales 3-12 mm/hrin 1MAle)
SAgluington
2 KBCCoAtains spea ati4¬AS heyae erpaieto_
echic 4J4lutiñS, Clungi of RBCako_Pldce_asa_
anbn_anthady ractimhsiS kauA aS_A4glutin4bon
ACTaN OF KBL.
Transgort_of oxygen_an Carondidid_
Maintanence_of_aGd bae_halane 10Aic_balanO an VOity
of blae
3) fomLuon of le_PianentS as a eult _of thcir
ithutio0 u retuloeadotkelial SySten
PARUL UNIVERSITY Ig. Na.: 2
FACULTY OF PHARMACY Date
PKAC TLCAL6
LenS
ATM
Bod buse
Revev No
uand Use_af ComPaund MicoScope
REQ DLREMEL)
Cosefrausing
MechonisM
objecove Dompauwd icroscope
REFEKENCC
ARM Ra and Rk hoYal NM_Pabel_ PratalAnatamy and
PhySialady, éducaba0Pull&ker_16Edhon
PYNoL B.S SAah_Prakashan_
hdubing
fiN fcsing
Mecnis ARS oFE CoMPOUND MICRaSCOPE
SStage
Base 2It COnsist oF hree Parts Ko sEand, te body
aN_bO rain Of 0ptica lenses
CoMPauNd MICKOSCOPE Á
A Suggor System
12 Base Tts a keavy_meballkc-shaped base_uhich
Supports fke_MiceSope_on_tÁe uwok tasle to_prev
Maximu Stalliy
Am o Cune hanwel_diih_Poieuts_bese_uhich
Sueportso FocusiN_a Mauhiuný SipttM
Ig.
Na.:
23)
PARUL
UNIVERSITY
FACULTY
OF
PHARMACY
Date
Bady
tuhe
Fitted
_at
the
lpper
en
aE
the
hale
eAk
Verhcally_ar_
at
aA
"anle
tda
hody
bube
LS
pazt
bhnugh
which
liadi_
palks
to_
tAO
eyePe
ce
hus
CoWlucbiAg_
EKZ
imaue
_to
oeye
oF
e
Obsevey
U
12SEge
E
ka
twp
comppnests_k
Fred
stage
Ad
Ha
Mezhaaicals
bage
CA)
hYeStAge
-
05pluak
platform
wi
an
apeabure
n
b
ente
ANd
Fhtted
to_olin
lelo
oliective
len
5)
Alchaical
sLaaL
reSa
SPnAq
maunted
cip
Lo
MauAted
/_
AZI2_
FN_SliA
BI
Flcusing
Sustem
FN
1
EAhcusing
MechanjsmIL
Maves
tdo
cptica
Sys
tRM_Or
daul
rpidlu
haugh_a
laýt
eAtance
Kack
and
nian
Suhstage:
TLis
Enploued
for
raising
ar
ldwe4
hCptic
lSsstEM
Wth
rtkte_to
twlide
_uader
stuokyil
it
comes
In
to
Focus
Che
ptical
CManuAg)System
lens
o
lens
fts
in
to
tho
tp
of
tde
bogu
bule
Lh
agnihe
da
image
OiecthVe
leaser
7hregPnAq
laade
akiictves
ol
edch
le
d
i
f
feyent
P
A
R
U
L
UNIVERSITY
Ig.
Ne.:(2
FACULTY
OF
PHARMACY
Dale
D
Noye
fiee
Il
eps
to
ralate
le
lens
int
any
desired
pgsution_
e
luniagtionystem
I)
Mrar
IE
Lolps
ta
divege_ayoF
light
and
died
t
AED
CancleASer
bo
Fokm_aElear
MaJe
PPLICAT
ION_OF
CoMPouw)_
MICROLORE
2
Planb
Cels
ad
MuChok4sMstructuecan
be
examing
out
wih
lp
af
Comgoud
Mcnscapye
To
blow
anglusis_under
taken_
uhich
is
of
area
use_in_
diag
Onisinq
ne.ss
2
Thus,
Compaund
Micnscape_
is
oE_grat
we
iA
atinlgy
laj
PARTS
aF
oMgOUN_KROSOPL
StuduralL
0phal
p
in
icnkaHe_
homn
hich
ue
lmk
into
MiChlae
hauing_
Maqnified
pouir
oF
bx.
Bave
for
Sugport
oE
MiLaicope
Am
which
Cannects_to
be
hae
and
Suptt
le
miinicape
hea
Or
eepiee--Sk
to
sax_
PARUL
UNIVERSITY
Date:
FACULTY
OF
PHARMACY
|2
Eye
piee
tube
e_tule_that
halds
bo
eye
pee_
n
Ahove_bo_obiecte
lens.
3
OliectiA
leas_
Aoy
allPma
Optical
lens
on2
MimpeAy
knge
homL
to
loox
an
ypally
iN
udé
t
3,WS
lensDA
MOE
oF_MICHHSQP
Stage
Stageis
a
plare
uhold
peamen
to
be
Viedeis_páced
Llumiaator
1
3
a
liaht
Saure_ol
MCnscepe
lacated
aA
Ko_base
_oE
Mitascope
(LEARNING
OVTomES
Compaun
micascpe
has
areat
use
Pathalogy
lah
By
ue_of
Compaund
MicnScope_e
cun_
detecdSce
Vey
minute
Pakticles
PARUL UNIVERsITY
FACULTY OF PHARMACY
Date
TRACLICA
SLmatian of impahhn CoNtEMt Of 2uh blad
REQUIREMEMES
2Sahlils haemaglahtoMeter_or hemometr which nsE 1
raduaR dlutin4 buleA thin 4ass ad a/ MALJEHE
OP Z culc_lmeter Lcmm_CaLadty_lh_LERz
Nlo Hcldstled unter 7a/ dok Dr 1d
ahyrhent Cotton
RE
FEREIMCES
aaual Rk_and DrPatelNM Practicalanatamy And
ste44 Lth_edtiol_lopi-20la
8.S Shah Prakahan,Ahnmedalad a a12-22
THEORy
a)lon_hload is Mxed_with MloHed RRC_ae haemalysel
an HB5 Rrzted 5 HB Cal/erted nto acid hehatn
wWhich 5esh_brauA in Calr
7he soSLilutel_wth_dstilled aattr tilt patáa wth
o_Stascard 4hss tule 7 HB/ Can dedly je fea
Tom R_qläduated tuse
PARUL
UNIVERSITY
FACULTY
OF
PHARMACY
Date
CKOCE
DURE
KoJaduatedlubing
ule
and
the
mirappe
bte_uee
Clealt
bhy&yghly
and
dne
2)
e
anduated_eilutin4
Lule
uas
Elld
wh
/lo
Hcl
epto
aÁ
t
b
e
3To
nger_uas
cleand
wth
1
alloral
anit
uwas
piked
bo
otA
a_dep_pE
blond
Ftst
arp
ual
pw
1ut
eCd
dhep
wasSucked
e
Mihpipetie
uLto
m
f
cac
The
lood
wasnmediately_deposited
at
be
battom_aE
graduated
tube
Ppetté_was
iASe
tuwp
ta
three
ms
in
HCL
S)
e
blood
was
ed
with_te
halp_af
sirer
en
Soluthon
was_allawed_to
tostand
For
lo
-
miAute
So
that
all
H
is
Canverted
into
ag
hematin.
To
mture
waston_diluted_with
dsbledater
Dtilled
water
wal
addedry
hy_drp
aN
lVey_orpLt
wa
shm
t
tho
xatnatch
with
Stngar
qlass
tube
wAs
dbtained
7
hen
e
matching_was
u/as_Comgletestumer
ua
taken_
out
fronL
the_cllitinq_
tuhe_adnd
he
SCale
wal
Rad
oA
tA_Side_of
the
Tg.
No.:
ZS)
P
A
R
U
L
UNIVERSITY
FACULTY
OF
PHARMACY
Date:
KRE
SVLL
7he
haemgalaluA
COntent
ot
my
un
blood
u
Foud
toLe
1241L
DSCUssION
2
e
Coloy
indey
s
deined
a
elative
_amaunL
oF
haem0qbbin
preseNE_
in
sinde
RBC
IE
can
bealy
detehiined
y
Fndn4_aut
te
ato_af
halMp
globi
cnd
RBC
U
2Calor
Lnder
=
Hs/RRC
Nomal
rauge_ak
calar
indez
saes
Lo
L.LS
I
calor
iAdex
is
aAaemia
IE
Col
indeis
mare
than
1.1S
i
s
deshhed
ss
han
.Rs
E
s
desled
as
hupechianic
aS
huPchmic
anaemi
PARUL
UNIVERSITY
Date
FACULTY
OF
PHARMACY
PRACT
ICAL-
KAIM
Deterninatdn
of
bleedng
time
of
ou
6la
REQUIRE
MEMIS
Steh
ledispasahle_pitking_Aeedle
Flter_papr
stof
watch
Co
tton
swa
o7
VIthano
oy
2o1
metnula
te
SPnht
CoPsuk
REFEREANCES
aual
Rk
and
Dr_Patel_NM_Practcal
anatany
_and
Phasioleay
Lh
edban
2a1-2alo
B.S
shahPrakahan
Abnedalud
Pa
m-
32-33
THEORY
2Bleeding
ine
_is
fime
nterualbehueen_skuA_
AMCEue_ad_Cessaton
DF
Youo
blood-
actoY
affectina_
kleedng_timë_
aye
phy
sial
size
and
shqge
of
n
VAculan
dotkelalsmaithnes
au
elasbcli,
al
blaad
VesSels,
Capila
Con
tnctilhSealng
by
plate
lets,Wunley
The
CaPllay_Conbacblity
asd
labelets_Play_an
imPor
tant
role
in
LCSSakionDf
Yleedig
7eae
yelatvely
SMgle
lechanis
ms
hon
o
Clotting
kace
et6nq
ime
Sholer
theñ
Clating
Lim
SOS1
s 071
Sob
0
so
SOE
1s
AOVWHVHd 10 AIinovi
ALISHIAINn 108Vd1
PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig. Ne.:31
Date
RACLICA-4
ALM
Detexnnaidn o clading hing_of aun_hlnad
REQVIREMENT)
DCapillayy_or sldls Cotton, to alahal_priklnylaecdle
KEfEREACEE2
Practical Aatans_anPhusidlagu RLhyal,
DrALMpatel,B.Shan_rakdshahithpdtion
PNCIPLL 22
Dunna_Loaaulaiol Sol faM_ef_llaad is chapgsd to
4 FEtm fK Lime Clapsed, hetueen_te matNtoE
LScape_pÉ blood autside_the_ese And t aksenahion2
o Phusial_change_ taken al clothina i
ne ehueen Alaod escape an eSsel_and fomation
C ibn is Called_clotbina bimg 1 4-lamA
I Cpillay CoAbacksyaAd PlateleEL_Pla4 A0
npay tant e in (eSsatibn OF Lleedn4_
9h0_dstuwlance_in_clotkingtiate may lead toVaA
dislase
Pg. No.: 32
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Date
OHaemo Philia 1EALh_ConditionCaused bs aksenc
o any factor reguirid Fe Clatting ie prolatgaienL
clsttn4 Lime_
Puguya S oNLitn assciated wih_platelet
deficiency AaPpensARD blood leakS om_lace vessels
Mrchanism_ot Coaquuhion-
O fomatian_of _thmmboplastin ken bl2ad Comes in
CoAtALtwith KOuah _Sufce_or_isue etact_plateleks_
disiAteAmtes andKumbaplastin is_q919ated_
fonaian oE themkin Te bhramboplasbn ntevacds_i_
plasna prothznmbin and thxanlkin_is_Fomed
foLmaban_of clot Thomkinfemed Convevts_Fhnagen
PRQCEDVAE
Te lipoE invey EipAer sherized_withZal alcahal
Aa sold_pick isaienSo that_bloadFlous Freely ze
StopuatA'Mnedialpty _stavte
e orl_of blood aeaimtely SMM_didneley is elaee
Asyecial_alass_ slile_Tho Ske_is held Veyically eveydoi_
Nobe the
Seen 1A_4y clottinq iML.
me uwhen no _change inshape ofdu
AOVWaVHd
10
AL1nov
2
ALISYIINN
TnUvd
Pg. No.: 34
PARUL UNIVERSITTY
FACULTY OF PHARMACY Date
TPRACI TCA-O
ATME
Deteminain_of heart-rate_A pulse-ratt.
REFEKENCE
Prar tiuu AAatams_and Pbusinlo4uLRL4pyal Dr
ApatelL BSShas _piakhan/ ed-2d -SZ
REQDIKEMENI
Awrist WAtch wih secands alM
HEORY
hot at.eSt rate f cnaction andelaxahun
2Ae 2ate h llau_af_bload frem the apila
which Can ay deteliad Wh_Din4EOn a
eart at£ is e Aumke df tne tke hea_
DEAtS w 1bin a_ce tain_enad_cF tMe Ako h/ ate
Can ie_RIt at he wrt Jidk_dE Pk, hadk eÉKARI
ponaa
h
AOVWJVHd
10
ALTnoV
ALISYIAINN
1
n
d
P A R U L UNIVERSITY
FACULTY OFPHARMACY
Ig. No.: 36
Date
VER
TPRACLICA-1/
To dekemind bload ressuNe_of_Sulied
E a r i E R M E T
P r a c h i u a l A N a t a n u _ a n u s i a l 4 y , r
R.khoyal Dr Natel
BSShah pakasiah Ana 2ol3-/4,An:4S-49_
REQIREIMEAUTS
2Stethascop and
1ezy>PAygmenaiIO.
THEORY
Pice
auscukatiol-1 LDASiIE_OE_
Lax hame CoASist_of tu_Mehallic LulsjonGd,Ly_
a lat CUNg Shfwhich_as a5 a 5ph to Pu
t etkerh ueperends_oF_ule_Eachte ha
per AenzunHl_Lmb which zuns medially doun_uarad
aN_EDWAK aNiM a_LUNature SULtakP
ted in tho_pktenal auetays meatul.
-Spudascj
2)
COnductng tahe They alo SimpleFlexhle_an¢foz
NSU kues_of_Julor_ar late mat9iul. hele tu
OANPCIS_ twCAst_piece eth the PaME_
Ig. Na.: 37
P A R U L UNIVERSITY
FACULTY OF PHARMACY Date :
3Cot Piece Zt OASLA OFMekallic tanlayr alat
cn iA diameter And hav4 ai qankeDE SAM-am
in degth 7k dAa4I1_Lhich_1SMate_uP of ellyase
aneliatian f SDundSulhich _are héaLMuCh buder
SPAuAnMmanMeter 1E3_Ao astunent ued_to Measue
Weun AogpessuH DE huma being_tconsitoF_
Moroier Meyry meter COSitOr tue lns ne_lalls _
loAA_
leAnduat lam0at th0_baie_o 2Saat
Sor_oE
atnasghind at
Vale hichNevenESpiln4_oF mezuy 7heari
liMis
Valye which allou ree Comuni.ahon_ q ul
Covekd lith anAtght lid lahtanjg_a
Kupiey bag wth lmin cuFEIi Co/ANr yaLnen
Cut is`4It_and elabc ndi htta wts twe pSA.
zalber ulng okokuhe &tt aith_MetdlAdafterad
othor_with air PUMP_
u Shart_
ke_atke Side_which _Puvided lwha V
VAlve
ThO linea Cuf aS a loud
ctensin 'on OL Side_and al tayna_
SpAug mon'anon eer
Metallic hoak.
PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 3P
Date :
ler pMp_wth ualE TiSaSmple_laic ualle
rller u AE OA_AVal ShALe ng a 'si2e that i
iAh b1t_n tuld DpeAIAq WhiCh al_Ftta
values
PKINCILE
The locd Elaw thhaygh a layae S20d arte s_dkstuctal
an pped_Cuouid Ehg la 7_rtSSHN Aslawly
réease
ouctiLA iS studkd o hasii OF_FeelngaF_2ulse_
tseriation d osdlatinn oE My level_Iedhg suith
Stethacepe_tho
tyfey 2ul to_dstuhon
aAd tho attnj At oE lacd thnuah
Sounes prdued hthe_ieametaF_the
Te bload Flou stogs wlea te_preSSure_baANALLKd h
boartey_bbuah 'e_huber "au equal_tag
MAN teLA thO_ble LressL_e_EK e of Iod
through an dlstuchen nicates th_loopessuve
PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig No.: 39
Date
POCEDIRE
TN suiect ulas asked to Sit oyhe doun_at eaeon
a CauchiA the SMPINe pItion_Ae Aamests tnud te
amy dye_emeyed'Ho an_wAl laa natabk and
E MsiLiOD wA_adisted Such that_it_wa at to
level oE A@at._
Tho cufF_way_biel aund_Ge aun It should le
neither top tignt to Cause_aAy Ccomfort totho subiect
ADr toa loose to allou its_idvenent_ouA HO am
Te _artey ulas felt _andits CAUBe was matke inHe
(ua_o`a.
2 Mapemeter _uwas placal y bhe side o e Sulyed
etuweenbis An_adbho_bug w OCAL_aftAO Julle
Punp wa tightened u k_Punlg wa_esedko
ate _te haq
whgn Lo_pulse_aear E AaLa 10ted _that_Mek
starts asillating_Ne DxtassillatiaA levela/e sita
NEas/A4 JMA4niteeAA then laullydninn Ze
EVelet_whick E0_osalavos iE i3 kncwNa
Dcillatgy Metha
Auscula toJy_mehed,dFter_ atlatenasuuz)
LchiaaLtoy ia th culital_fosY Ad cAFANA
Saly lkasa4 tA_peeZ lad al
ADVWaVHd
10
AL70oV
ALISHIAINN
7nYVd
G
PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig. No.: 4I
Date:
UL UNIVVE
PRACL1CAIZZ
Delanination_oF bladAraup
REFEKENCE
Prachal AnAtm_ Andphusialaay r 4eualDrM
pabe BSshdh fprakhshaln 1shedilldA2:8F-Lpana-2a
REQDIREMEAL)
Alicnxapic slid6 4lass mathin4_pencL dnhstm-AAiua2
and Antisen-D Pnckin_ nèeale, to alohal and michce
PKTNCTPIEE
AKonin4 to_ABO_43ten_AE blood_a/2lfiA4 tereCan
eFou blaedas A BAA hdaU
Te_Ani-Sed A_COnSist_aE agalutnin_alpha_aAd Lt
CaS clumging dE RBC AE HOAdCntanq 44914tg
A
AntiSeaSera B_COASAE_oF aAglutiain_jeta anclut
Lauses_ dumeing_o BC _E Lad coAtainna aglutuge.
B_tt_Can'_aktained asfola/-
ant..
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 42
Date
Ant:SeM A B
DCRHD
Dduning
in_AlL-R_ADload Araup A
cluaphg
2clungingin_ab-SenB Rloadap A_
clungina iN_Anb-SenA,B> Bload ahupAR
U
No-clunying Clo_cluMgina_
inany Sem2 Bloczd 4aypO
Adbing anti-sta A foms ClumpngaLonina hKE"System
TE RH foMS_cluMPIng_lood 4p> te
Aing anbi-sera B No Clunping
I RH doesnt fom clunpn loe2graup--Ve.
Aving anti-5e oms dunypna.
PROCEDURE)
DTake hree_slidesand mark theM ARadD_
1)Place ypeAseuM_on [5t_dat oF blaod
upe- an secod hp_ot_Dland 1ype-on
Ehi drdp_aE blod
32Steane_lefgne that_ae_prk tAe Finge_and fut_
axp4F load_en slie
Mix it wth e matchStick.
SAlldul t For SMiA_
ramiAL_afLer Smin Clunspp4.
9V
UDi'ssDSITT
9
vydiy
uugnbhy
obnliy
AOVWaVHd
10
ALTnOVA
ALISA3AINN
7
N
Y
V
d
PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg.Ne.: 44
Date:
VERS
PRATI CALB
ALM-)
To cany alt He eyEancyle_Sedmentation_rate
REFERENCE
Pracical Anatoms _and Physidlegy,DzRkhpyalL La_
Palel BSShakpakashanKE edEiA 20 4
REQNTREMENT
Westeynenul 0.1MSazuM_Ctnte
Pranciple
T SR_istho_ate at_which ec_loa_cels
SPneAt AaRhdd
Aenatalo44 test Ang a AASPeCiFL_Méasure of
AFlanatidr Mazmally Yed blad celSSettleAlatvely slau
fateY thanYlbmal Yate May0dLate AAnnatin
T PahLAt_ESR_ ishigh it mau_lehte to iannidi
kdney diease_
UHeart degse aoumatau_arthins
PARUNIVERSITY
FACULTY OF PHARMACY
Pg. No.: S
Date:
onpbimesto 2SKan leSlaulez_than_Mimal
ASlauw LK_MAy_ALLAte_A aad diiavdr_SuCh_as
PoluCytAnia_SihLL CPN AMnialeukecqtasis
ST nt4_ramei
i)_:22naL_Foz M and
0-29mm farwamP
As the ythrogyte Sf/MeAL_IA_a_pPhiod_efone_
stage st lomiA
1t iniual 0 entatina iso-
unng whih
initial_penidd of a44regationn
kAuleaur Are ome a SrMentatian ilou_
Stag 2-Mex 0 Mu
Isa Periada fastSetlnySedmentaten
OCCUYa ' CoAstant ratLe_duzaa I_pPhdz
stage3
ASentLaion_g4ain slau_a
aib_JAal_jQzizd
packia4 D celsat te bal~un_aE tie thle_
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 46
Date :
L L K O C E D I U R E
Wihdau LmlF VevausJlad
Then mixed eract lo M_ Sodium_Gbate ulith Lal
aF VAACus bloodin 4_tule
DInderbad te_Euje 23 2Mes to mixed Ke_lood
thkoughly wth tnt-Loa4ulant
Fl LkO WAleqen tule up to mabiy and
place iA at_E_0o teMPeLzture_M awAy
hom Sunliaht
lake e_eudngs _eCactly_afterjAy Katord io
mihibe_em k Saface_dE Colum to ZpaF
KESDLL-
AO ythincyte_5ed
SedmeAtation_Yate i) ound_Zo
PVo
WnIV
TPVOTT
TPTDO-T
TRDdW3FT
(
W
L
Y
AOVWaVHd
10
ALinOVH
ALISYIAINN
7nYVd
Vibron
PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 49
Date :
are tuld CAULtUAS=ontalSinwe
e CaenalS4tUK PntalEfaietl
JamhaidalSutueCoonalsuturei oCalAanc
SauamaS SutuN /¬mf0lalt_pahctal
D Mailla S20 df head Len
fallewo h ah twe allenh han_and seft-
mala T OWaal a tua_Ahal bon
CAONChAe
Olacial- pcatee i ye LhKh sejkte)_ujan
A L4S mal holein_(OnAr -(
e aw ALA Mandhle -O
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PARUL UNIVERSITY EXPERIMENT ON HUMAN BODY TISSUES

  • 1. PARUL UNIVERSITY ND EX Experiment/TBe s.NO Gate Teachers Sgpaturs Marks Dosby Epthelal and COnnEve bissuL oE uman body 2J o study MasulaY and Nentus bis oF Aunan b S-2 3JTnbaducbonbo Haemogytamey 19-12 Debeminatien oF uhibebland ellCwl) DE own blaod B- 16 S1DeteminabonoF tobal Kad_bload cell CRB COunltoF ownlaod 17-2
  • 2. PARUL UNIVERSITY FACULTY OF PHARMACY Ig. Na.: 0 Dale UNINL PRACTICA-L AM To StulyLpitkalial and Comeckive issue_aE Auman bau YREFERENCE CaOUa Rk _an Dr PatelN.M Practical Anatogy_and PSialoauL edihan Zoo1-Zalo_BS Sha pakashan Akmedkid_Pa Na- 99-lag 0-B.S ShaDakas KREQUIREM ENTS2 charbsSLies_ PDESCRIAIIaN SPBalial Tissues Tho ypes oF cayennq_andhnin4 epitelial hssu are classried atordingto two aacteristic armlgemant of Lells into layes al o sh4pe) OF__ Cels A AragenNnl.ofcelsinto layer'and theshapeEbhe cells. a Simple epiikalwm
  • 3. PARUL UNIVERSITY FACULTY OF PHARMACY Ig.Na.: Date - a SinplepLmItis aSiAgle lauerof cels bhat_ Funchiansin dHisSiA OSMasis,tnan, Seetinn o ahsopian Secebon is thoPrnductian an leaje af SubsEanceSALh_ MuK S/eat,OY en2ymes t6104phA isho iaEakt_0E BHuids_Dr Athor SubstHnces Such 45 dgeste ood renAO intALinal Eact Sin ple SauoM.auS SbetifFied epVann Cuboidal PeudhstakER_Rptbalun AppeAs_to_haVe_multle layes of CelS Decaue Be relAucle le_at_dFftvent lels AAd AaE allcels exten to 4pcal_Suface my contain lia o he Caahlek cells Selrabs_MuCAS PeldastxabliEzed_ephelnuais achualy a siMple_epitalu hecase al ilscelk_restan_ tho bakenint_nembin. reach the opica SurFae_Cells tatdo Smpe Cuboidal Sbacifi loidalA Glumal cstaified epatkelum Conssts E tun Or mphe ayes o Cells that pntect nderlyin_bissuo_in_locatinswhore Hore 15 LbAsilerable weakan tear ololo90o|0 BICellshapehe Lell Vayinslape_epalinq_onair FUAcbion Sinpe columar peun Starihtd una aSuamausCalls axt ahagt lhte Flanr tiles_a1dak_thn whilh allaus_o R_teíipalsaqe OF Suhstance luhadal cel anasLalas Hayak_w andak shapa ie Cuhe Y horagons y_Mat hale Micoull_alher Apica SurFace an Functhan etAor Secvetion_ar dhsarphion
  • 4. PARUL UNIVERSITY FACULTY OF PHARMACY Pg Na.: Date: cCalumAAr cellsat much baller han boy are wide lke CduMAS and pntect underlyin tisSuos Tor apical Surfaces May_ANO_Cilia nicovill and 'thy ofte a Specialiedor secHLion and ahsarption 1 ransLtidna cel Chanae shre on Flat to_cuhadaland 6aaSoAASuch 4sho unuy bladoer sbeck tD naer Size ad Lan collapse a maltr sizæ DGecause_of te_diueriy_oF celk_and etracelularmabir and hediFFerencPs in Beir relativP prapartions the lossi fiation o COnnertVe tissugs otaluays dlear-ut and SeVëal claSEcatiin_Pis i)omecb/e TissusS -ep Nuceus mbyyan Conneckie bssun A Mesencheme B Mucav Connective bissu 2)MabuH CoAnechue tissue Rehalar ti BDenseCnedtive tsu A) Lonse Conmectwe bsu Avealar oAhie tSu DDase_reaular su Fbsikt c Dene ingauhybstug Colagen Tg 3 Eashr ctibsu 3)otuhr comedise tsu Flrelet ee
  • 5. j , PARUL UNIVERSITY FACULTY OF PHARMACY Ig.Na.: Date: cioiog DIBonvtösue E Lauidtastcbieba Filole arulage auoe Bload tissu alinCazhlage 2lymph Fhncaztilage 31lasbc (alage reylay ConMeivehis Cpsael MAOL Corlge cs Flaolye #Cartlg EoicA Melb COmi RL Bloo BonA
  • 6. PARUL UNIVERSITY FACULTY OF PHARMACY Ig.Na.- Date: PRACTICA KAME 10 study_MusculaY and Nenhus bisuoE Auman hady VREFERENCE aOual Kkald Dr_LatelM.M_Prattiul Anatamyand PAMsialag y tatian Loni-2alh_B.S Shah Prakashan Akomuaad Pa no 1-h2_ EREOUIREMETS) Doarts sldes LOSRIPIION-2 )Muscuar bissu askalebalMNcle Sskeletal muscle SS is Aome For its latatian-itis Sualattrkad bo the honus OE he skeleban AntHer Feabu i its Stiationsalternatip lah and dakhand withnthe Eihes thab are _isihleundter a liaht mcd Dheletal Mude iCansiered Valultayeause t Canle Made to conbac or elak bu CiSCiaus CoADal kalaa MMsdle_Ers_con_ Vay in letatK rom a Feu/Cm_Ln shartmuscles to uph 30-4b inupur longatMucle.
  • 7. PARUL UNIVERSTY FACULTY OF PHARMACY Date SAIMusdle Fheris raughly culiadrita inShapeand has maly Aucle lacate at ptrighory ithin a uhaleMUscle tho ndiuidual usde Ehres may he Panll0_bo_a0 angther celauc Smooth musale Smanth muscle Lssuois lacatedin_thewallsOE hollo inLenalS1MELsSuch 2S hlaad /essek airuAys h_ h ungS Stanach, intestines4al-jladder and U i t a r y a d e l e r Is ConbncEian hip Consbid or Aayaw He lumen_aFE Anad vesel Pusially break daun and mk_faad aloA_ aasbainteabiaal_tat mave Fluids_hiagh thadsar eimates /astes SMayh Mede SMathMusclo_Eh a uSually intalunbay and hau are on-sbriaked,heace the_bezm SManbh A_SMath Mede ERY ISSmall, thickest iA W Middle Qd tapentg at each ead IL onkinsa Sih4le cenbaly locateAclod p jundtians CanMect Maly indilidual Fhes n SMaath Musde tssw Far ea nho walL_oE heiAtetnes Such muscle tissugan_praduce_PoweFul_Contachdn as Many MusclO Fhes COntaCE in_UAon_n_athez lncatidns Sugh al [Ko hS0F IKO eye smaath Musclehe Conbract individually likaSkaletalAcleIAs_Llcase Aap ulchons ake alieat_
  • 8. PARUL UNIVERSITY FACULTY OF PHARMACY Date CardiA Musale Canlia Muscloe bsu orms mskaf ble walla£ heheakt Luke skoleal Muscle it iS sbaated However unlike_SkalebalMusdebissuo tis nvalue t5 coALrattidn 1S An1 ConSCiauly contralled Puced Cariac Ascle_ibres_are_bzanchad and uiually have LenDnillylacated Aucleus_AN DCtSiana end to end_by bansveAe plaima MemhzanCalled nteralabed isS hith CoAtan_both desmnsames ad hap_junctians I ce has uwa Audle Aou attah hick enip4S_dE Conac Muge Dhalated dC ake uAiqIu ta Cakdiac Musde_ssw h deOMOSamOS Stenqtkin h_ss 2nd hld_a Fbres 7hoap Junctions piuide_a nute For OF Mulle actidn Potenbial thnughaut L hond togetho_duiAG teir VjgOraus COAtactians 4uick Condudn iMenaus hissua Despibe the awesomo_complexb aFthe Nerloussysten CoAsists_af only tulo pincigal ap AFcellkueglia. andNeuHnnS euEaA And ne es a SeMSitive to Vahausstimul Koy Convert stmuhinto_elechial Scanals CiNductheso actidn bteatials to ather "eulani t Muscde tissues ta_qlands Caled atton_PDtentials WveiMpase) and
  • 9. PARUL UNIVERSITY FACULTY OF PHARMACY Date MosL NthOnS_CoSLL af rte_basic paslare badu and wo knds af Cell puesedwne afons 7ke cell_oy contains the Auleus_omd ottis O 4 a n e l e Dendate)_are tagring huahly branchedd UuallySue CellproLeSs They ard A najor eceula iu Apat SinglethCylndatal_ Prncess that may e_veyleg STEisthe Output ition af a utanLolduting 0enimpulses bewar annlher Aeunl or totame oer tissuO_ yen bough ARULglhia_d Aat pinLnte Av Ccnduct Nerue iMfyeS 1thase LelS do Ae Maly MLLtnt Supparti Eunhons_AniM4 Placed hat plate thd at_plate wWl AdE a 4 NoLAuÍ St/mulahen
  • 10. PARUL UNIVERSITY FACULTY OF PHARMACY Dale PRAC TICAL Tatreductian to Hemacytometpy (REQULREMENIS 9 I>HaemoCytometAr St uhithCOASiSLS OF NuhauLyS counta ChanherBLPptte, Ihonas calirslf Lulhl_ald MiLastnpe DREFERENCES aONal Rkan Ur latelNiM Prattical aAutamy_and Plustolagy lhedstion, Zoa-2ola BSshah _}akashan- THEORY TAo COuAtia/cells_CKBCorWBC) in_Aload usinq haemocy tome set_is Caled as haemofytomat Tho umherot_Lls_in te_blooz a_too MaAy aA4 tho S2 OE Cell s too Small Tke_cells w apar in cluster E Seen_bhznugh_MiLLoxape ThiS difikull S partlyoVerrome dluting_Lland to knnuA_degret 2 HatmgcyhaunmuttrcoASstof iDluAing Pipettes i)Cauntiua Chamk (ecial CaVeslp-
  • 11. PARUL UNIVERsITY FACULTY OF PHARMACY Date ie Diluian Ppats DIL Cansist_aE Eour_parls-Thelang stemhe hulh,th Shortstem_and and the Sucker The lan4 StemAa a Unulon_capillazy bore tteding fomawell Around ConiLAl up And meain4 inthe hualh The long s£eM isdided into lo Ezd Pozts Fom_tho EY to t malh 1ust neay"tAg bulk Rulerbde Bul Beat Mgulh Piece JteM The FEth 2wisidn iskeauly _maked and laleledasd.s_ This_pat IS o Mei exact amount o blood takenForCounting o Fluid iA this_part does Aat bake Payt in tho2lutiaA a1d eALe this quantty M _deducted wh.l0_calalating thediuin Fr s -CafilbyjoN e bulh is part wlere t_eilubiano blood Lake_pluce It COnSst oF whte or edhead uhith kolps_in uAifbm MiriAg Of blaad with_2lution COAical Ei Fluid The_ RBC ppetbe WBC pifette ulb2Ads ina_shprt stem which have_the 11ar lol he Shart stem alo cansist_of_ulu+n Caploy bore_ Lt hoy red lead I t ha ehte bead Calilbatian 0-S,1 any Calatian- 0.5, L0 TAo sucker is_Flbed to tlo_eAd_oF Short stem_7 Sucker 1s nbher or_Palytlong tule Attde end of huhe bore is toplasic mauth Piece and lot Sze of lalb ib lupger Size df bulß is sanadle Maih fiece is red ntolbur Mach ece is hie inday 8lunon Can be dln upb bu, Diluti on (an be cone vpto 1D0 or Zoo tmes
  • 12. PARUL UNIVERSITY FACULTY OF PHARMACY Dale Counting_Chamler. 1E i5a Sngle piece thickalass slide hauiA4w COuniAg-aFta iA_CeAtalpart Iha tuD Cohti4g artas ave e fakate iam eah ober _part oF SM y an Hshape araolL_on tho eitbor sije_ai kse Aendsmal are platforms uhich are nised y 0.Lmm area ahoue to_Cauninqarea Onthee plalfams_zeLes SPecia Cauersliphamas Coversli CantalnZ-15 Tho ulin4 areasof Chanlher_are tao shap_CoAtast Ae laire oF_brighLoes a4ainst thedarker_Metahai hakqmun ARA JE 1e A Ai semibranparent laser oE the_melal_h& heen degsibed pA tho4laK Countingared and tho_ liAes_ar hled pu hraug USE aS OA COAAat SeeEeStais dunb4 the 2hu Be ulia Canat be Seen in hnghi L4hEs Focddd DA thechamher_ hob-0,002SMman o dhal Surfe Volune ofer a larre Aur CArea-dert oime F0.ul 2lonljust the chamheroe_must partinlyclase tke diaphram_ oF te hicaso2e 0-looomm 2 e takal_aling ared_oY each slide is `nnn length an 3mnin Headth T isdaided intoAiAe _tqual SAuars DF 1smm_ared Th bondazs_lines oF Bese Stuares Ae bnplelinings 0-0025M2 Ne uaul ste fouS4uarts oF COLneIS Ve used For wBC Counting_azhie Sfud I) For RBC COUntin
  • 13. PARUL UNIVERSITY FACULTY OF PHARMACY 2 equal small KRC s4uares,and lacn al thae 73Smal REL SmallestSpuAAy Sngle hn4 hus uhale Cenbal Square diyide no hon SMallet hWluiea flud VaiauS_lutian Flus a Se n AALmacytam2y hut te hasic Chtlna or_peparil4 bo_Hbo fu tat it skaul ke iytani td plam To compasitianDE dlutial Flui depud oA dtbr Yeaiteménts such_a StaitiA4 cion e Tahlo7 an temast_Commanly usedilution Fluis ior KB 4 And uWBLCaunt wth purtalke af each cOnsztun
  • 14. PARUL UNIVERSITY FACULTY OF PHARMACY Date PRACTICALL SALM Eauneation of h1be_blood_Cell_Count of 6uA bload. KREQUIREMENTS COASshS of NeulauLerls Countog HALMactometerSet_ ulhizk chamber WAL pipete Thonas coverslip,uwatch alass, WB dutina Elud Pickling Aeedle 201:allohal fal an michige EEEREALE Goual k _and pr_laklwM Prchical Amtarmyapd PAsialaay edition,2os1-2olo Bs shah _pritkahan, Pmedaaa laMa I-3 ETHEOKy Mumber_aF uhite Bload cells in hlood ae tod may and e sine_af cedsis too Snal T i Korefore | Adt gassikle a_caunt tho cells euen under Agh pouk his dificulty is_jaztialy avercone u dilutitge alaod ih a_Sultahle_dlutionFluid toa kKoun deare Te diluted Alond is_placed iA a capllay spale or kndA APaci etaleen counting chamberd Cove Slp- Te Auber_of Cels iA thO_SMall_capllas SPACe_oF knvwN_ /nlune is ten Couated uade b ighPouje at MiCosLoLe Tho CoAt Can_1 Catrulated hymulkplurg
  • 15. YL PARUL UNIVERSITY FACULTY OF PHARMACY Dule: hoAumber wb dlution tactor,DIsuyhance A e pf Lone ondposure i e Lht sAL o MaunLanence LenLellyMedaleySKleLalMude relacat.on 2Compasulion of wBC D/lation fluz. Glacial ALeicau 20ml 2Desboy kEC henhan ar MethylValetU/) 10ml2S2uns AudeotwR Wabey upto Lwm Dluent PROCE DURE2 e homas coVealip,Lountung_chamber and be_lenses oE MCnsage are leantd ir ith the_Aip_aylal 2 Tho thamas CouAhagchanher uasacdiusted a oheNd Ader lou paulr E_IaoPe_keLpA4_ti_thamai CoVeslP sty 0A toplallarm af slide 2 e ng Finger wa sterized wuth tho alahal and t_ wAS phkedhadly uih tte helpE pritkhag_Aeede e Fist dnp_o blaod u!as hp Was SuIked_ia bo WRpipette up to tdoMak oS inmeJiately _be W/BC_lutián Flui Mak disarded a e Secend a Scke_up 12 pitle_uwas nled bet paln o mir Rlacd.audl ful
  • 16. m y P A R U L U N I V E R S I T Y FACULTY OF PHARMACY Dale: lew desare distavde and bun pgelte wasd a a4glß oF 4to HosurfaLe_af ounbinr Caner AA Lpwas Aplied bo be_ NanowSLEJELue 7A2 COMting Chambera be coerslp >(CALCULA[TO 3 S3 6 3 B 7 The_Eluid was_alauted to settle nr hre niAutes e sta4e of miosape 32 6 42 8 e WRC damjer uias locate and WBCS,aL COUnted in Mallest1 sAuaes AS wa epeated iA_anatser yeeSuch such_Chamhes 2 7 33 2 3 2RESVLT2 T BC Caun_ AF My_dun 6land uwa aund Lo le_2.20/mM A-9 8:77, C-43, V-3| wBC: No. of celx DF pd OISaSS TaNV Caunted 2SSX 2o Artax Depth 4xO. GeAeral chaactersties dE WB.C-Leuocye are wandehng cels whih 2re_Audleated_aA d lot Contaiany haemaglokin_Tkey a Shghtly_biager in Sine ad_sn_Aun~ haiongin is erakasala braAulgyle_ae dened Foled onMarau uh1le Aaaulatute _are dehuked spleen ao umph4lands Tee 2SSx So Cmm l2.7So /Cmm fe spanistoo shart Tew haus to Feu daus 2Nosal wßC Cautis hgon-_lloco_lcmm IFKe coun goes belou L-00nlcmm it iS knaun as leucopenia Beydleucacy
  • 17. PARUL UNIVERSITY FACULTY OF PHARMACY Date ynctian_of WBL Phajoglaus Tt i engulEing cf forign_Mattna- Backek D foreian_pirbiclE 22Anthady_Famaton.Antihadia a hamma-hloubns fomein ryanse t invasian Anyanjii Funchitn is iame autympaddybes 3 Formatian ofFibzellasts wB playsan iMporte role in_ bssue hepair A inFlanmattun mphsatee are 4enWrally oñVEed_ntp_tdee_ Ljkhntan_of ALtamiAe_anz All2ak eLtian £DsinDghil ah ChieFly_iAualv n thie reactians SSeetiol_at AgAn Hepnhn san anticnaq1lant Basphs ak 4aWally hih in_Aepahil
  • 18. V PARUL UNIVERSITY FACULTY OF PHARMACY Dale ZPRACLICAL54 DAIM EAuMerat.idn al tatal RBCault al OW lacd EQUIREMENLS Neuhauer CauntiA4 Chamber KBL Pipetbe mai Caver sp,Watch4lassRBC 2uta4 Fluid Pnklng APelelol allahal yldl and iLadpE REFERENCE- DGayal Kkand Dr latel_MM attdlAnatun an phySidloqu_l edtin_2o01-20lO. B.Sshah prakushdn_2Amedalad Pa A4-22-2S e_Aumber_oF rdcels in lood_ae tan maAyan S12e_of Lells s_to0 SmalLt iSBoreore_AAE Posszale t Caunt tho cell euen_UAder hi4h POwe hs _dfficulby is_partial_avercane_hu luting tae slan with aS talle_dlybian Eluig a khoun_2eake y _dluted blod is phted in a_capillansSpatt_EKALUN Capaci ty A etueen CounM_ChapiheanCClor sl kaguA Volume is ika (auAteuniep K higa outr
  • 19. INa1 PARULUNIVERSITY FACULTY OF PHARMACY Date: O_aunt can Je calculat y Multgly.lg Awniber ulith the dlatianfuctar Suhstande Amaunt Puipe 2 Spdiumchlgnde 2PreLent ncly Praviie pteniaty revents ouler oML 2Ssdhum_Suphaior Mercuric Chlaridle SAn 2CaKe atiinafLell wAter ptalnm PROCEDURE DTe tomas caverslit, Caunbugchamhey and tho ene of MiCtascage_uQre cleane ikt h the help_ Te thamas CouAtingchamher ue aduitedan_obserd forKBCsphaNSUAder_lou_Pouer of Micokafg Keep04_ toIhoma CQUASliP r0tin4ah the Platfakn a HeKe slhe_ ddiute for Aigh Pau and chamder ofBC Sak au
  • 20. P A R U L UNIVERSITY FACULTY OF PHARMACY Dale: 7 KE pipttle / cleaned ao di h M4 wa Styhza wi e_Alconl A1 Y Ae fust_ap_af_blocdwa 4LAd a TA SecMd dropwas SuCkin KL pifette upio imnediatply e RBL 2luLonFlui was Sucked up taMalbl heLLHe_was bizughi to a Áon2aAtalzaiti pipetteAGMple kaht was_41/A tothe ubuer tale ePiPeLtP_WA led betu/len ThO_Pulm_oMik_ h hlond wthIA_zlutian Fluid for_aN MAuP 2 feydrapS_ave_dscaded_and then_Pipette_was hela atho aale oE s°tothe surface DF LountiA4 etuen te cputing haMbEY_ an tiO CoVeslp_ 2 70 Fuid was aloued to Settle for_thre MiAute on stage_ MiL1ce. 7e RB humher was located and KBLsAe(ounted inMalest6 14uare.hS WSy LefeateZ_In_ aAather aur sdch chambes
  • 21. eMA, PARUL UNIVERSITY FACULTYOF PHARMACY TRESLIS 7 LBC COut dE 4_aun Alanz ws faund i he S09o.aonlca YDISCOSSIO B 2 had_carpsdes are ciuar hcancaeLad-Audete cek Tey ConLan Epnaiony Panint_taenagioial. Aomal RL3 121n dianeier ad 221 AAe RBCNo OF celcounbexDilutionacdr KDepth xea Tg are thaner in centre thuSAfeahjg_kedum leb Shaped RBL Formein gleen a l h enbp n Dalts RBL FOMMA in rihone maimu = Nxloo xloxS So9 x lo000 DfOmatian RL isknguin as eathngitys So9ooo0/Cmm RBC shau5 Variusprgorhes- Haemalusis 1FRBCi5kept in huprtaiz_Salutian 5wells aad Einalynes Eating haEMOahlin ThISiskopuA_ Haemdluis KaularFumationL StPngerty_pE KRCtO CDME Susfindi_1A_pläna os lag asndi fekg_
  • 22. P A R U L U N I V E R S I T Y F A C U L T Y O F P H A R M A C Y Date ylbagyle Sednentalion pat TE KBC d_allaved to stand an anb-C24ulatan Supenata0t 2SR_3_defined_as MMOF t lear lasMA Fameda ho top ot yeztial ColuM i0 a Aour t isrecnal oF SuSPenSiOn s tely 2ESR-_tmm/hauy ales 3-12 mm/hrin 1MAle) SAgluington 2 KBCCoAtains spea ati4¬AS heyae erpaieto_ echic 4J4lutiñS, Clungi of RBCako_Pldce_asa_ anbn_anthady ractimhsiS kauA aS_A4glutin4bon ACTaN OF KBL. Transgort_of oxygen_an Carondidid_ Maintanence_of_aGd bae_halane 10Aic_balanO an VOity of blae 3) fomLuon of le_PianentS as a eult _of thcir ithutio0 u retuloeadotkelial SySten
  • 23. PARUL UNIVERSITY Ig. Na.: 2 FACULTY OF PHARMACY Date PKAC TLCAL6 LenS ATM Bod buse Revev No uand Use_af ComPaund MicoScope REQ DLREMEL) Cosefrausing MechonisM objecove Dompauwd icroscope REFEKENCC ARM Ra and Rk hoYal NM_Pabel_ PratalAnatamy and PhySialady, éducaba0Pull&ker_16Edhon PYNoL B.S SAah_Prakashan_ hdubing fiN fcsing Mecnis ARS oFE CoMPOUND MICRaSCOPE SStage Base 2It COnsist oF hree Parts Ko sEand, te body aN_bO rain Of 0ptica lenses CoMPauNd MICKOSCOPE Á A Suggor System 12 Base Tts a keavy_meballkc-shaped base_uhich Supports fke_MiceSope_on_tÁe uwok tasle to_prev Maximu Stalliy Am o Cune hanwel_diih_Poieuts_bese_uhich Sueportso FocusiN_a Mauhiuný SipttM
  • 24. Ig. Na.: 23) PARUL UNIVERSITY FACULTY OF PHARMACY Date Bady tuhe Fitted _at the lpper en aE the hale eAk Verhcally_ar_ at aA "anle tda hody bube LS pazt bhnugh which liadi_ palks to_ tAO eyePe ce hus CoWlucbiAg_ EKZ imaue _to oeye oF e Obsevey U 12SEge E ka twp comppnests_k Fred stage Ad Ha Mezhaaicals bage CA) hYeStAge - 05pluak platform wi an apeabure n b ente ANd Fhtted to_olin lelo oliective len 5) Alchaical sLaaL reSa SPnAq maunted cip Lo MauAted /_ AZI2_ FN_SliA BI Flcusing Sustem FN 1 EAhcusing MechanjsmIL Maves tdo cptica Sys tRM_Or daul rpidlu haugh_a laýt eAtance Kack and nian Suhstage: TLis Enploued for raising ar ldwe4 hCptic lSsstEM Wth rtkte_to twlide _uader stuokyil it comes In to Focus Che ptical CManuAg)System lens o lens fts in to tho tp of tde bogu bule Lh agnihe da image OiecthVe leaser 7hregPnAq laade akiictves ol edch le d i f feyent
  • 27. PARUL UNIVERsITY FACULTY OF PHARMACY Date TRACLICA SLmatian of impahhn CoNtEMt Of 2uh blad REQUIREMEMES 2Sahlils haemaglahtoMeter_or hemometr which nsE 1 raduaR dlutin4 buleA thin 4ass ad a/ MALJEHE OP Z culc_lmeter Lcmm_CaLadty_lh_LERz Nlo Hcldstled unter 7a/ dok Dr 1d ahyrhent Cotton RE FEREIMCES aaual Rk_and DrPatelNM Practicalanatamy And ste44 Lth_edtiol_lopi-20la 8.S Shah Prakahan,Ahnmedalad a a12-22 THEORy a)lon_hload is Mxed_with MloHed RRC_ae haemalysel an HB5 Rrzted 5 HB Cal/erted nto acid hehatn wWhich 5esh_brauA in Calr 7he soSLilutel_wth_dstilled aattr tilt patáa wth o_Stascard 4hss tule 7 HB/ Can dedly je fea Tom R_qläduated tuse
  • 28. PARUL UNIVERSITY FACULTY OF PHARMACY Date CKOCE DURE KoJaduatedlubing ule and the mirappe bte_uee Clealt bhy&yghly and dne 2) e anduated_eilutin4 Lule uas Elld wh /lo Hcl epto aÁ t b e 3To nger_uas cleand wth 1 alloral anit uwas piked bo otA a_dep_pE blond Ftst arp ual pw 1ut eCd dhep wasSucked e Mihpipetie uLto m f cac The lood wasnmediately_deposited at be battom_aE graduated tube Ppetté_was iASe tuwp ta three ms in HCL S) e blood was ed with_te halp_af sirer en Soluthon was_allawed_to tostand For lo - miAute So that all H is Canverted into ag hematin. To mture waston_diluted_with dsbledater Dtilled water wal addedry hy_drp aN lVey_orpLt wa shm t tho xatnatch with Stngar qlass tube wAs dbtained 7 hen e matching_was u/as_Comgletestumer ua taken_ out fronL the_cllitinq_ tuhe_adnd he SCale wal Rad oA tA_Side_of the
  • 31. SOS1 s 071 Sob 0 so SOE 1s AOVWHVHd 10 AIinovi ALISHIAINn 108Vd1
  • 32. PARUL UNIVERSITY FACULTY OF PHARMACY Ig. Ne.:31 Date RACLICA-4 ALM Detexnnaidn o clading hing_of aun_hlnad REQVIREMENT) DCapillayy_or sldls Cotton, to alahal_priklnylaecdle KEfEREACEE2 Practical Aatans_anPhusidlagu RLhyal, DrALMpatel,B.Shan_rakdshahithpdtion PNCIPLL 22 Dunna_Loaaulaiol Sol faM_ef_llaad is chapgsd to 4 FEtm fK Lime Clapsed, hetueen_te matNtoE LScape_pÉ blood autside_the_ese And t aksenahion2 o Phusial_change_ taken al clothina i ne ehueen Alaod escape an eSsel_and fomation C ibn is Called_clotbina bimg 1 4-lamA I Cpillay CoAbacksyaAd PlateleEL_Pla4 A0 npay tant e in (eSsatibn OF Lleedn4_ 9h0_dstuwlance_in_clotkingtiate may lead toVaA dislase
  • 33. Pg. No.: 32 P A R U L UNIVERSITY FACULTY OF PHARMACY Date OHaemo Philia 1EALh_ConditionCaused bs aksenc o any factor reguirid Fe Clatting ie prolatgaienL clsttn4 Lime_ Puguya S oNLitn assciated wih_platelet deficiency AaPpensARD blood leakS om_lace vessels Mrchanism_ot Coaquuhion- O fomatian_of _thmmboplastin ken bl2ad Comes in CoAtALtwith KOuah _Sufce_or_isue etact_plateleks_ disiAteAmtes andKumbaplastin is_q919ated_ fonaian oE themkin Te bhramboplasbn ntevacds_i_ plasna prothznmbin and thxanlkin_is_Fomed foLmaban_of clot Thomkinfemed Convevts_Fhnagen PRQCEDVAE Te lipoE invey EipAer sherized_withZal alcahal Aa sold_pick isaienSo that_bloadFlous Freely ze StopuatA'Mnedialpty _stavte e orl_of blood aeaimtely SMM_didneley is elaee Asyecial_alass_ slile_Tho Ske_is held Veyically eveydoi_ Nobe the Seen 1A_4y clottinq iML. me uwhen no _change inshape ofdu
  • 35. Pg. No.: 34 PARUL UNIVERSITTY FACULTY OF PHARMACY Date TPRACI TCA-O ATME Deteminain_of heart-rate_A pulse-ratt. REFEKENCE Prar tiuu AAatams_and Pbusinlo4uLRL4pyal Dr ApatelL BSShas _piakhan/ ed-2d -SZ REQDIKEMENI Awrist WAtch wih secands alM HEORY hot at.eSt rate f cnaction andelaxahun 2Ae 2ate h llau_af_bload frem the apila which Can ay deteliad Wh_Din4EOn a eart at£ is e Aumke df tne tke hea_ DEAtS w 1bin a_ce tain_enad_cF tMe Ako h/ ate Can ie_RIt at he wrt Jidk_dE Pk, hadk eÉKARI
  • 37. P A R U L UNIVERSITY FACULTY OFPHARMACY Ig. No.: 36 Date VER TPRACLICA-1/ To dekemind bload ressuNe_of_Sulied E a r i E R M E T P r a c h i u a l A N a t a n u _ a n u s i a l 4 y , r R.khoyal Dr Natel BSShah pakasiah Ana 2ol3-/4,An:4S-49_ REQIREIMEAUTS 2Stethascop and 1ezy>PAygmenaiIO. THEORY Pice auscukatiol-1 LDASiIE_OE_ Lax hame CoASist_of tu_Mehallic LulsjonGd,Ly_ a lat CUNg Shfwhich_as a5 a 5ph to Pu t etkerh ueperends_oF_ule_Eachte ha per AenzunHl_Lmb which zuns medially doun_uarad aN_EDWAK aNiM a_LUNature SULtakP ted in tho_pktenal auetays meatul. -Spudascj 2) COnductng tahe They alo SimpleFlexhle_an¢foz NSU kues_of_Julor_ar late mat9iul. hele tu OANPCIS_ twCAst_piece eth the PaME_
  • 38. Ig. Na.: 37 P A R U L UNIVERSITY FACULTY OF PHARMACY Date : 3Cot Piece Zt OASLA OFMekallic tanlayr alat cn iA diameter And hav4 ai qankeDE SAM-am in degth 7k dAa4I1_Lhich_1SMate_uP of ellyase aneliatian f SDundSulhich _are héaLMuCh buder SPAuAnMmanMeter 1E3_Ao astunent ued_to Measue Weun AogpessuH DE huma being_tconsitoF_ Moroier Meyry meter COSitOr tue lns ne_lalls _ loAA_ leAnduat lam0at th0_baie_o 2Saat Sor_oE atnasghind at Vale hichNevenESpiln4_oF mezuy 7heari liMis Valye which allou ree Comuni.ahon_ q ul Covekd lith anAtght lid lahtanjg_a Kupiey bag wth lmin cuFEIi Co/ANr yaLnen Cut is`4It_and elabc ndi htta wts twe pSA. zalber ulng okokuhe &tt aith_MetdlAdafterad othor_with air PUMP_ u Shart_ ke_atke Side_which _Puvided lwha V VAlve ThO linea Cuf aS a loud ctensin 'on OL Side_and al tayna_ SpAug mon'anon eer Metallic hoak.
  • 39. PARUL UNIVERSITY FACULTY OF PHARMACY Pg. Na.: 3P Date : ler pMp_wth ualE TiSaSmple_laic ualle rller u AE OA_AVal ShALe ng a 'si2e that i iAh b1t_n tuld DpeAIAq WhiCh al_Ftta values PKINCILE The locd Elaw thhaygh a layae S20d arte s_dkstuctal an pped_Cuouid Ehg la 7_rtSSHN Aslawly réease ouctiLA iS studkd o hasii OF_FeelngaF_2ulse_ tseriation d osdlatinn oE My level_Iedhg suith Stethacepe_tho tyfey 2ul to_dstuhon aAd tho attnj At oE lacd thnuah Sounes prdued hthe_ieametaF_the Te bload Flou stogs wlea te_preSSure_baANALLKd h boartey_bbuah 'e_huber "au equal_tag MAN teLA thO_ble LressL_e_EK e of Iod through an dlstuchen nicates th_loopessuve
  • 40. PARUL UNIVERSITY FACULTY OF PHARMACY Ig No.: 39 Date POCEDIRE TN suiect ulas asked to Sit oyhe doun_at eaeon a CauchiA the SMPINe pItion_Ae Aamests tnud te amy dye_emeyed'Ho an_wAl laa natabk and E MsiLiOD wA_adisted Such that_it_wa at to level oE A@at._ Tho cufF_way_biel aund_Ge aun It should le neither top tignt to Cause_aAy Ccomfort totho subiect ADr toa loose to allou its_idvenent_ouA HO am Te _artey ulas felt _andits CAUBe was matke inHe (ua_o`a. 2 Mapemeter _uwas placal y bhe side o e Sulyed etuweenbis An_adbho_bug w OCAL_aftAO Julle Punp wa tightened u k_Punlg wa_esedko ate _te haq whgn Lo_pulse_aear E AaLa 10ted _that_Mek starts asillating_Ne DxtassillatiaA levela/e sita NEas/A4 JMA4niteeAA then laullydninn Ze EVelet_whick E0_osalavos iE i3 kncwNa Dcillatgy Metha Auscula toJy_mehed,dFter_ atlatenasuuz) LchiaaLtoy ia th culital_fosY Ad cAFANA Saly lkasa4 tA_peeZ lad al
  • 42. PARUL UNIVERSITY FACULTY OF PHARMACY Ig. No.: 4I Date: UL UNIVVE PRACL1CAIZZ Delanination_oF bladAraup REFEKENCE Prachal AnAtm_ Andphusialaay r 4eualDrM pabe BSshdh fprakhshaln 1shedilldA2:8F-Lpana-2a REQDIREMEAL) Alicnxapic slid6 4lass mathin4_pencL dnhstm-AAiua2 and Antisen-D Pnckin_ nèeale, to alohal and michce PKTNCTPIEE AKonin4 to_ABO_43ten_AE blood_a/2lfiA4 tereCan eFou blaedas A BAA hdaU Te_Ani-Sed A_COnSist_aE agalutnin_alpha_aAd Lt CaS clumging dE RBC AE HOAdCntanq 44914tg A AntiSeaSera B_COASAE_oF aAglutiain_jeta anclut Lauses_ dumeing_o BC _E Lad coAtainna aglutuge. B_tt_Can'_aktained asfola/-
  • 43. ant.. P A R U L UNIVERSITY FACULTY OF PHARMACY Pg. Na.: 42 Date Ant:SeM A B DCRHD Dduning in_AlL-R_ADload Araup A cluaphg 2clungingin_ab-SenB Rloadap A_ clungina iN_Anb-SenA,B> Bload ahupAR U No-clunying Clo_cluMgina_ inany Sem2 Bloczd 4aypO Adbing anti-sta A foms ClumpngaLonina hKE"System TE RH foMS_cluMPIng_lood 4p> te Aing anbi-sera B No Clunping I RH doesnt fom clunpn loe2graup--Ve. Aving anti-5e oms dunypna. PROCEDURE) DTake hree_slidesand mark theM ARadD_ 1)Place ypeAseuM_on [5t_dat oF blaod upe- an secod hp_ot_Dland 1ype-on Ehi drdp_aE blod 32Steane_lefgne that_ae_prk tAe Finge_and fut_ axp4F load_en slie Mix it wth e matchStick. SAlldul t For SMiA_ ramiAL_afLer Smin Clunspp4.
  • 45. PARUL UNIVERSITY FACULTY OF PHARMACY Pg.Ne.: 44 Date: VERS PRATI CALB ALM-) To cany alt He eyEancyle_Sedmentation_rate REFERENCE Pracical Anatoms _and Physidlegy,DzRkhpyalL La_ Palel BSShakpakashanKE edEiA 20 4 REQNTREMENT Westeynenul 0.1MSazuM_Ctnte Pranciple T SR_istho_ate at_which ec_loa_cels SPneAt AaRhdd Aenatalo44 test Ang a AASPeCiFL_Méasure of AFlanatidr Mazmally Yed blad celSSettleAlatvely slau fateY thanYlbmal Yate May0dLate AAnnatin T PahLAt_ESR_ ishigh it mau_lehte to iannidi kdney diease_ UHeart degse aoumatau_arthins
  • 46. PARUNIVERSITY FACULTY OF PHARMACY Pg. No.: S Date: onpbimesto 2SKan leSlaulez_than_Mimal ASlauw LK_MAy_ALLAte_A aad diiavdr_SuCh_as PoluCytAnia_SihLL CPN AMnialeukecqtasis ST nt4_ramei i)_:22naL_Foz M and 0-29mm farwamP As the ythrogyte Sf/MeAL_IA_a_pPhiod_efone_ stage st lomiA 1t iniual 0 entatina iso- unng whih initial_penidd of a44regationn kAuleaur Are ome a SrMentatian ilou_ Stag 2-Mex 0 Mu Isa Periada fastSetlnySedmentaten OCCUYa ' CoAstant ratLe_duzaa I_pPhdz stage3 ASentLaion_g4ain slau_a aib_JAal_jQzizd packia4 D celsat te bal~un_aE tie thle_
  • 47. P A R U L UNIVERSITY FACULTY OF PHARMACY Pg. Na.: 46 Date : L L K O C E D I U R E Wihdau LmlF VevausJlad Then mixed eract lo M_ Sodium_Gbate ulith Lal aF VAACus bloodin 4_tule DInderbad te_Euje 23 2Mes to mixed Ke_lood thkoughly wth tnt-Loa4ulant Fl LkO WAleqen tule up to mabiy and place iA at_E_0o teMPeLzture_M awAy hom Sunliaht lake e_eudngs _eCactly_afterjAy Katord io mihibe_em k Saface_dE Colum to ZpaF KESDLL- AO ythincyte_5ed SedmeAtation_Yate i) ound_Zo
  • 49. Vibron PARUL UNIVERSITY FACULTY OF PHARMACY Pg. Na.: 49 Date : are tuld CAULtUAS=ontalSinwe e CaenalS4tUK PntalEfaietl JamhaidalSutueCoonalsuturei oCalAanc SauamaS SutuN /¬mf0lalt_pahctal D Mailla S20 df head Len fallewo h ah twe allenh han_and seft- mala T OWaal a tua_Ahal bon CAONChAe Olacial- pcatee i ye LhKh sejkte)_ujan A L4S mal holein_(OnAr -( e aw ALA Mandhle -O Facial ond O Ai pe in aoth uhih nly nl
  • 50. brant P A R U L UNIVERSITY FACULTY OF PHARMACY Date: kll all al consist_d 2ar_bad- Malleus Mlle Stapes MuS C) Stape C) -Sma/le t_ an Awloy asicdej RIRS TAe_dhe total z4 rib f Stemun wN 17_L_kAwAal_tL hls_because ii_dzedlyattach t0 Stenum la_al_ kaau as falße nis hetale s Aoiectly attach fo Stezum Stenun ale_kntun aS ilaatingzls betaue they ale Flaating nat attacK dh sten 2h /ertebral_coloumn Consist otal C2)_one O Cervic ConSAA OF tota '-bon0 PeSLAEeA lhe_Wee_ aAd dhene abe 2'Ehdkaisi _jcl heloy_CenicaCobu_L
  • 54. e V i b r a n t . P A R U L UNIVERSITY FACULTY OF PHARMACY Pg. Me. 53 Date : llna i Medialong_sf to _foreayn anad loA4PrLAan 1A0radius 1 (onsst_ shot loey iHenLtie eplr end OF Uha is stznA aAd Ak lKe StutiuUR_wih lauge Pevis Hh7Jot Shage ilt_andond Wi Gongt dE Cagal Bones dE bnnes7s_a requlafShapAd_bones_a/ arhulate) Cnur with_ne a n d i r hC ans OF_alalS Scagholunate tinbal, -Pakla _Pfpm, bapl20idCaptate_an aAZ hamate MEtaapal bIne are iA_D0Ne_ Pun len_aiulae with istalkou_ 2 Capel_hano_A ths_is Etahlah etter Facliiy_oE the p jaint wkh__ Troala Fibaa Tavsa Mererasa phoilanye h Lhia_ard bula= uaA Tlula o the l resemAles adiul an_ulna of dhl_oH -ap_ he 1ower end Zilia is the Sma malile dsC iO fomKa knet CapP- DONe ot foot_lere ave_SeVen_baal bonesStuattyD- l ous and ulp tohearLho weight E TA_04p ve melalhaal_hane laW y L Phalang e Anl2 Fr areat_toe A Ach_Eodt _