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PARUL UNIVERSITY EXPERIMENT ON HUMAN BODY TISSUES
1. PARUL UNIVERSITY
ND EX
Experiment/TBe
s.NO Gate Teachers
Sgpaturs
Marks
Dosby Epthelal and
COnnEve bissuL oE
uman body
2J o study MasulaY and
Nentus bis oF
Aunan b
S-2
3JTnbaducbonbo
Haemogytamey
19-12
Debeminatien oF
uhibebland ellCwl)
DE own blaod
B-
16
S1DeteminabonoF tobal
Kad_bload cell CRB
COunltoF ownlaod
17-2
2. PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig. Na.: 0
Dale
UNINL
PRACTICA-L
AM
To StulyLpitkalial and Comeckive issue_aE Auman
bau
YREFERENCE
CaOUa Rk _an Dr PatelN.M Practical Anatogy_and
PSialoauL edihan Zoo1-Zalo_BS Sha pakashan
Akmedkid_Pa Na- 99-lag
0-B.S
ShaDakas
KREQUIREM ENTS2
charbsSLies_
PDESCRIAIIaN
SPBalial Tissues
Tho ypes oF cayennq_andhnin4 epitelial hssu are
classried atordingto two aacteristic
armlgemant of Lells into layes al o sh4pe) OF__
Cels
A AragenNnl.ofcelsinto layer'and theshapeEbhe cells.
a Simple epiikalwm
3. PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig.Na.:
Date
- a SinplepLmItis aSiAgle lauerof cels bhat_
Funchiansin dHisSiA OSMasis,tnan, Seetinn o
ahsopian Secebon is thoPrnductian an leaje af
SubsEanceSALh_ MuK S/eat,OY en2ymes t6104phA
isho iaEakt_0E BHuids_Dr Athor SubstHnces Such 45
dgeste ood renAO intALinal Eact
Sin ple SauoM.auS SbetifFied epVann
Cuboidal
PeudhstakER_Rptbalun AppeAs_to_haVe_multle layes
of CelS Decaue Be relAucle le_at_dFftvent lels
AAd AaE allcels
exten to 4pcal_Suface my contain lia o he
Caahlek cells Selrabs_MuCAS PeldastxabliEzed_ephelnuais
achualy a siMple_epitalu hecase al ilscelk_restan_
tho bakenint_nembin.
reach the opica SurFae_Cells tatdo
Smpe Cuboidal Sbacifi loidalA
Glumal cstaified epatkelum Conssts E tun Or mphe ayes o
Cells that pntect nderlyin_bissuo_in_locatinswhore
Hore 15 LbAsilerable weakan tear
ololo90o|0 BICellshapehe Lell Vayinslape_epalinq_onair
FUAcbion
Sinpe columar peun Starihtd una aSuamausCalls axt ahagt lhte Flanr tiles_a1dak_thn
whilh allaus_o R_teíipalsaqe OF Suhstance
luhadal cel anasLalas Hayak_w andak shapa
ie Cuhe Y horagons y_Mat hale Micoull_alher
Apica SurFace an Functhan etAor Secvetion_ar
dhsarphion
4. PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg Na.:
Date:
cCalumAAr cellsat much baller han boy are wide lke
CduMAS and pntect underlyin tisSuos Tor apical
Surfaces May_ANO_Cilia nicovill and 'thy ofte
a
Specialiedor secHLion and ahsarption
1 ransLtidna cel Chanae shre on Flat to_cuhadaland
6aaSoAASuch 4sho unuy bladoer sbeck tD
naer Size ad Lan collapse a maltr sizæ
DGecause_of te_diueriy_oF celk_and etracelularmabir
and hediFFerencPs in Beir relativP prapartions the
lossi fiation o COnnertVe tissugs otaluays dlear-ut
and SeVëal claSEcatiin_Pis
i)omecb/e TissusS
-ep
Nuceus
mbyyan Conneckie bssun
A Mesencheme B Mucav Connective bissu
2)MabuH CoAnechue tissue
Rehalar ti
BDenseCnedtive tsu
A) Lonse Conmectwe bsu
Avealar oAhie tSu DDase_reaular su
Fbsikt c
Dene ingauhybstug
Colagen Tg
3 Eashr ctibsu
3)otuhr comedise tsu
Flrelet ee
5. j , PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig.Na.:
Date:
cioiog DIBonvtösue E Lauidtastcbieba
Filole arulage
auoe Bload tissu
alinCazhlage
2lymph
Fhncaztilage
31lasbc (alage
reylay ConMeivehis
Cpsael
MAOL
Corlge
cs
Flaolye
#Cartlg EoicA
Melb
COmi
RL
Bloo
BonA
6. PARUL UNIVERSITY
FACULTY OF PHARMACY Ig.Na.-
Date:
PRACTICA
KAME
10 study_MusculaY and Nenhus bisuoE Auman hady
VREFERENCE
aOual Kkald Dr_LatelM.M_Prattiul Anatamyand
PAMsialag y tatian Loni-2alh_B.S Shah Prakashan
Akomuaad Pa no 1-h2_
EREOUIREMETS)
Doarts sldes
LOSRIPIION-2
)Muscuar bissu
askalebalMNcle
Sskeletal muscle SS is Aome For its latatian-itis
Sualattrkad bo the honus OE he skeleban AntHer
Feabu i its Stiationsalternatip lah and dakhand
withnthe Eihes thab are _isihleundter a liaht mcd
Dheletal Mude iCansiered Valultayeause t Canle
Made to conbac or elak bu CiSCiaus CoADal kalaa
MMsdle_Ers_con_ Vay in letatK rom a Feu/Cm_Ln
shartmuscles to uph 30-4b inupur longatMucle.
7. PARUL UNIVERSTY
FACULTY OF PHARMACY Date
SAIMusdle Fheris raughly culiadrita inShapeand
has maly Aucle lacate at ptrighory ithin
a uhaleMUscle tho ndiuidual usde Ehres may
he Panll0_bo_a0 angther
celauc
Smooth musale
Smanth muscle Lssuois lacatedin_thewallsOE hollo
inLenalS1MELsSuch 2S hlaad /essek airuAys h_
h ungS Stanach, intestines4al-jladder and
U i t a r y a d e l e r
Is ConbncEian hip Consbid or Aayaw He lumen_aFE
Anad vesel Pusially break daun and mk_faad
aloA_ aasbainteabiaal_tat mave Fluids_hiagh
thadsar eimates /astes
SMayh Mede
SMathMusclo_Eh a uSually intalunbay and hau
are on-sbriaked,heace the_bezm SManbh A_SMath Mede
ERY ISSmall, thickest iA W Middle Qd tapentg at
each ead IL onkinsa Sih4le cenbaly locateAclod
p jundtians CanMect Maly indilidual Fhes n SMaath
Musde tssw Far ea nho walL_oE heiAtetnes
Such muscle tissugan_praduce_PoweFul_Contachdn
as Many MusclO Fhes COntaCE in_UAon_n_athez
lncatidns Sugh al [Ko hS0F IKO eye smaath Musclehe
Conbract individually likaSkaletalAcleIAs_Llcase
Aap ulchons ake alieat_
8. PARUL UNIVERSITY
FACULTY OF PHARMACY Date
CardiA Musale
Canlia Muscloe bsu orms mskaf ble walla£
heheakt Luke skoleal Muscle it iS sbaated
However unlike_SkalebalMusdebissuo tis nvalue
t5 coALrattidn 1S An1 ConSCiauly contralled
Puced
Cariac Ascle_ibres_are_bzanchad and uiually have
LenDnillylacated Aucleus_AN DCtSiana
end to end_by bansveAe
plaima MemhzanCalled nteralabed
isS hith CoAtan_both desmnsames ad hap_junctians
I ce has uwa Audle Aou attah
hick enip4S_dE
Conac Muge
Dhalated dC ake uAiqIu ta Cakdiac Musde_ssw
h deOMOSamOS Stenqtkin h_ss 2nd hld_a
Fbres
7hoap Junctions piuide_a nute For
OF Mulle actidn Potenbial thnughaut L hond
togetho_duiAG teir VjgOraus COAtactians
4uick Condudn
iMenaus hissua
Despibe the awesomo_complexb aFthe Nerloussysten
CoAsists_af only tulo pincigal ap AFcellkueglia.
andNeuHnnS euEaA And ne es a SeMSitive to
Vahausstimul Koy Convert stmuhinto_elechial
Scanals
CiNductheso actidn bteatials to ather "eulani
t Muscde tissues ta_qlands
Caled atton_PDtentials WveiMpase) and
9. PARUL UNIVERSITY
FACULTY OF PHARMACY Date
MosL NthOnS_CoSLL af rte_basic paslare
badu and wo knds af Cell puesedwne
afons 7ke cell_oy contains the Auleus_omd ottis
O 4 a n e l e
Dendate)_are tagring huahly branchedd UuallySue
CellproLeSs They ard A najor eceula iu Apat
SinglethCylndatal_
Prncess that may e_veyleg
STEisthe Output ition af a utanLolduting
0enimpulses bewar annlher Aeunl or totame
oer tissuO_
yen bough ARULglhia_d Aat pinLnte Av Ccnduct
Nerue iMfyeS 1thase LelS do Ae Maly MLLtnt
Supparti Eunhons_AniM4 Placed hat plate
thd at_plate wWl AdE a 4 NoLAuÍ St/mulahen
10. PARUL UNIVERSITY
FACULTY OF PHARMACY Dale
PRAC TICAL
Tatreductian to Hemacytometpy
(REQULREMENIS 9
I>HaemoCytometAr St uhithCOASiSLS OF NuhauLyS counta
ChanherBLPptte, Ihonas calirslf Lulhl_ald
MiLastnpe
DREFERENCES
aONal Rkan Ur latelNiM Prattical aAutamy_and
Plustolagy lhedstion, Zoa-2ola BSshah _}akashan-
THEORY
TAo COuAtia/cells_CKBCorWBC) in_Aload usinq
haemocy tome set_is Caled as haemofytomat
Tho umherot_Lls_in te_blooz a_too MaAy aA4
tho S2 OE Cell s too Small Tke_cells w apar
in cluster E Seen_bhznugh_MiLLoxape ThiS difikull
S partlyoVerrome dluting_Lland to knnuA_degret
2 HatmgcyhaunmuttrcoASstof iDluAing Pipettes i)Cauntiua
Chamk (ecial CaVeslp-
11. PARUL UNIVERsITY
FACULTY OF PHARMACY
Date
ie Diluian Ppats
DIL Cansist_aE Eour_parls-Thelang stemhe hulh,th
Shortstem_and
and the Sucker
The lan4 StemAa a Unulon_capillazy bore tteding
fomawell Around ConiLAl up And meain4 inthe
hualh The long s£eM isdided into lo Ezd Pozts
Fom_tho EY to t malh 1ust neay"tAg bulk
Rulerbde
Bul
Beat
Mgulh
Piece
JteM
The FEth 2wisidn iskeauly _maked and laleledasd.s_
This_pat IS o Mei exact amount o blood
takenForCounting o Fluid iA this_part does Aat
bake Payt in tho2lutiaA a1d eALe this quantty
M _deducted wh.l0_calalating thediuin Fr
s
-CafilbyjoN e bulh is part wlere t_eilubiano blood Lake_pluce
It COnSst oF whte or edhead uhith kolps_in
uAifbm MiriAg Of blaad with_2lution
COAical Ei
Fluid The_
RBC ppetbe WBC pifette ulb2Ads ina_shprt stem which have_the 11ar
lol he Shart stem alo cansist_of_ulu+n
Caploy bore_
Lt hoy red lead I t ha ehte bead
Calilbatian 0-S,1
any
Calatian- 0.5, L0 TAo sucker is_Flbed to tlo_eAd_oF Short stem_7
Sucker 1s nbher or_Palytlong tule Attde end of
huhe bore is toplasic mauth Piece
and lot
Sze of lalb ib lupger Size df bulß is sanadle
Maih fiece is red ntolbur Mach ece is hie inday
8lunon Can be dln upb bu,
Diluti on (an be cone vpto
1D0 or Zoo tmes
12. PARUL UNIVERSITY
FACULTY OF PHARMACY Dale
Counting_Chamler.
1E i5a Sngle piece thickalass slide hauiA4w
COuniAg-aFta iA_CeAtalpart Iha tuD Cohti4g artas
ave e fakate iam eah ober _part oF SM y
an Hshape araolL_on tho eitbor sije_ai kse
Aendsmal
are platforms uhich are nised y 0.Lmm
area
ahoue to_Cauninqarea Onthee plalfams_zeLes
SPecia Cauersliphamas Coversli
CantalnZ-15
Tho ulin4 areasof Chanlher_are tao shap_CoAtast
Ae laire oF_brighLoes a4ainst thedarker_Metahai hakqmun
ARA
JE 1e A Ai semibranparent laser oE the_melal_h&
heen degsibed pA tho4laK Countingared and tho_
liAes_ar hled pu hraug
USE aS OA COAAat SeeEeStais dunb4 the 2hu
Be ulia Canat be Seen in hnghi L4hEs Focddd
DA thechamher_
hob-0,002SMman o dhal Surfe
Volune ofer a larre Aur
CArea-dert oime
F0.ul
2lonljust the chamheroe_must partinlyclase tke
diaphram_ oF te hicaso2e
0-looomm 2 e takal_aling ared_oY each slide is `nnn length
an 3mnin Headth T isdaided intoAiAe _tqual
SAuars DF 1smm_ared Th bondazs_lines oF Bese
Stuares Ae bnplelinings
0-0025M2
Ne
uaul ste fouS4uarts oF COLneIS Ve used For wBC Counting_azhie
Sfud I) For RBC COUntin
13. PARUL UNIVERSITY
FACULTY OF PHARMACY
2 equal small KRC s4uares,and lacn al thae
73Smal REL
SmallestSpuAAy Sngle hn4
hus uhale Cenbal Square diyide no hon SMallet
hWluiea flud
VaiauS_lutian Flus a Se n AALmacytam2y
hut te hasic Chtlna or_peparil4 bo_Hbo fu
tat it skaul ke iytani td plam
To compasitianDE dlutial Flui depud oA dtbr
Yeaiteménts such_a StaitiA4 cion e Tahlo7 an
temast_Commanly usedilution Fluis ior KB
4
And uWBLCaunt wth purtalke af each cOnsztun
14. PARUL UNIVERSITY
FACULTY OF PHARMACY Date
PRACTICALL
SALM
Eauneation of h1be_blood_Cell_Count of 6uA bload.
KREQUIREMENTS
COASshS of NeulauLerls Countog
HALMactometerSet_ ulhizk
chamber WAL pipete Thonas coverslip,uwatch alass, WB
dutina Elud Pickling Aeedle 201:allohal fal an michige
EEEREALE
Goual k _and pr_laklwM Prchical Amtarmyapd
PAsialaay edition,2os1-2olo Bs shah _pritkahan,
Pmedaaa laMa I-3
ETHEOKy
Mumber_aF uhite Bload cells in hlood ae tod may
and e sine_af cedsis too Snal T i Korefore
| Adt gassikle a_caunt tho cells euen under Agh pouk
his dificulty is_jaztialy avercone u dilutitge alaod
ih a_Sultahle_dlutionFluid toa kKoun deare Te diluted
Alond is_placed iA a capllay spale or kndA APaci
etaleen counting chamberd Cove Slp-
Te Auber_of Cels iA thO_SMall_capllas SPACe_oF knvwN_
/nlune is ten Couated uade b ighPouje at
MiCosLoLe Tho CoAt Can_1 Catrulated hymulkplurg
15. YL PARUL UNIVERSITY
FACULTY OF PHARMACY Dule:
hoAumber wb dlution tactor,DIsuyhance A e
pf Lone ondposure i e Lht sAL o
MaunLanence
LenLellyMedaleySKleLalMude relacat.on
2Compasulion of wBC D/lation fluz.
Glacial ALeicau 20ml 2Desboy kEC
henhan ar MethylValetU/) 10ml2S2uns AudeotwR
Wabey upto Lwm Dluent
PROCE DURE2
e homas coVealip,Lountung_chamber and be_lenses
oE MCnsage are leantd ir ith the_Aip_aylal
2 Tho thamas CouAhagchanher uasacdiusted a oheNd
Ader lou paulr E_IaoPe_keLpA4_ti_thamai
CoVeslP sty 0A toplallarm af slide
2 e ng Finger wa sterized wuth tho alahal and t_
wAS phkedhadly uih tte helpE pritkhag_Aeede
e Fist dnp_o blaod u!as
hp Was SuIked_ia bo WRpipette up to tdoMak
oS inmeJiately _be W/BC_lutián Flui
Mak
disarded a e Secend
a Scke_up
12 pitle_uwas nled bet paln o mir Rlacd.audl ful
16. m y P A R U L U N I V E R S I T Y
FACULTY OF PHARMACY
Dale:
lew desare distavde and bun pgelte wasd a
a4glß oF 4to
HosurfaLe_af
ounbinr Caner
AA Lpwas Aplied bo be_
NanowSLEJELue 7A2
COMting Chambera be coerslp
>(CALCULA[TO
3 S3
6 3 B
7 The_Eluid was_alauted to settle nr hre niAutes
e sta4e of miosape
32 6
42
8 e WRC damjer uias locate and WBCS,aL COUnted
in Mallest1 sAuaes AS wa epeated iA_anatser
yeeSuch
such_Chamhes
2 7
33
2 3
2RESVLT2
T BC Caun_ AF My_dun 6land uwa aund
Lo le_2.20/mM
A-9 8:77, C-43,
V-3|
wBC: No. of celx DF pd
OISaSS TaNV
Caunted 2SSX 2o
Artax Depth 4xO. GeAeral chaactersties dE WB.C-Leuocye are
wandehng cels whih 2re_Audleated_aA d lot
Contaiany haemaglokin_Tkey a Shghtly_biager in
Sine ad_sn_Aun~ haiongin is erakasala
braAulgyle_ae dened Foled onMarau uh1le
Aaaulatute _are dehuked spleen ao umph4lands
Tee
2SSx So Cmm
l2.7So /Cmm
fe spanistoo shart Tew haus to Feu daus
2Nosal wßC Cautis hgon-_lloco_lcmm IFKe coun goes
belou L-00nlcmm it iS knaun as leucopenia Beydleucacy
17. PARUL UNIVERSITY
FACULTY OF PHARMACY Date
ynctian_of WBL
Phajoglaus Tt i engulEing cf forign_Mattna-
Backek D foreian_pirbiclE
22Anthady_Famaton.Antihadia a hamma-hloubns
fomein ryanse t invasian Anyanjii
Funchitn is iame autympaddybes
3 Formatian ofFibzellasts wB playsan iMporte role
in_ bssue hepair A inFlanmattun mphsatee
are 4enWrally oñVEed_ntp_tdee_
Ljkhntan_of ALtamiAe_anz All2ak eLtian
£DsinDghil ah ChieFly_iAualv n thie reactians
SSeetiol_at AgAn Hepnhn san anticnaq1lant
Basphs ak 4aWally hih in_Aepahil
18. V PARUL UNIVERSITY
FACULTY OF PHARMACY Dale
ZPRACLICAL54
DAIM
EAuMerat.idn al tatal RBCault al OW lacd
EQUIREMENLS
Neuhauer CauntiA4 Chamber KBL Pipetbe mai
Caver sp,Watch4lassRBC 2uta4 Fluid Pnklng
APelelol allahal yldl and iLadpE
REFERENCE-
DGayal Kkand Dr latel_MM attdlAnatun
an phySidloqu_l edtin_2o01-20lO. B.Sshah
prakushdn_2Amedalad Pa A4-22-2S
e_Aumber_oF rdcels in lood_ae tan maAyan
S12e_of Lells s_to0 SmalLt iSBoreore_AAE Posszale
t Caunt tho cell euen_UAder hi4h POwe hs
_dfficulby is_partial_avercane_hu luting tae slan
with aS talle_dlybian Eluig a khoun_2eake y
_dluted blod is phted in a_capillansSpatt_EKALUN
Capaci ty A etueen CounM_ChapiheanCClor sl
kaguA Volume is ika (auAteuniep K higa outr
19. INa1
PARULUNIVERSITY
FACULTY OF PHARMACY Date:
O_aunt can Je calculat y Multgly.lg
Awniber ulith the dlatianfuctar
Suhstande Amaunt Puipe
2 Spdiumchlgnde
2PreLent ncly
Praviie pteniaty
revents ouler oML
2Ssdhum_Suphaior
Mercuric Chlaridle SAn 2CaKe atiinafLell
wAter ptalnm
PROCEDURE
DTe tomas caverslit, Caunbugchamhey and tho ene
of MiCtascage_uQre cleane ikt h the help_
Te thamas CouAtingchamher ue aduitedan_obserd
forKBCsphaNSUAder_lou_Pouer of Micokafg
Keep04_ toIhoma CQUASliP r0tin4ah the Platfakn
a HeKe slhe_
ddiute for Aigh Pau and chamder ofBC Sak au
20. P A R U L UNIVERSITY
FACULTY OF PHARMACY Dale:
7 KE pipttle / cleaned ao di h M4
wa Styhza wi e_Alconl A1 Y
Ae fust_ap_af_blocdwa 4LAd a TA
SecMd dropwas SuCkin KL pifette upio
imnediatply e RBL 2luLonFlui
was Sucked up taMalbl
heLLHe_was bizughi to a Áon2aAtalzaiti
pipetteAGMple kaht was_41/A tothe ubuer tale
ePiPeLtP_WA led betu/len ThO_Pulm_oMik_
h hlond wthIA_zlutian Fluid for_aN MAuP
2 feydrapS_ave_dscaded_and then_Pipette_was hela
atho aale oE s°tothe surface DF LountiA4
etuen te cputing haMbEY_ an tiO CoVeslp_
2 70 Fuid was aloued to Settle for_thre MiAute on
stage_ MiL1ce.
7e RB humher was located and KBLsAe(ounted
inMalest6 14uare.hS WSy LefeateZ_In_
aAather aur sdch chambes
21. eMA, PARUL UNIVERSITY
FACULTYOF PHARMACY
TRESLIS
7 LBC COut dE 4_aun Alanz ws faund i
he S09o.aonlca
YDISCOSSIO
B 2 had_carpsdes are ciuar hcancaeLad-Audete
cek Tey ConLan Epnaiony Panint_taenagioial.
Aomal RL3 121n dianeier ad 221 AAe
RBCNo OF celcounbexDilutionacdr
KDepth xea Tg are thaner in centre thuSAfeahjg_kedum leb
Shaped RBL Formein gleen a l h enbp n
Dalts RBL FOMMA in rihone maimu
= Nxloo xloxS
So9 x lo000 DfOmatian RL isknguin as eathngitys
So9ooo0/Cmm RBC shau5 Variusprgorhes-
Haemalusis 1FRBCi5kept in huprtaiz_Salutian
5wells aad Einalynes Eating haEMOahlin
ThISiskopuA_ Haemdluis
KaularFumationL StPngerty_pE KRCtO CDME
Susfindi_1A_pläna os lag asndi fekg_
22. P A R U L U N I V E R S I T Y
F A C U L T Y O F P H A R M A C Y
Date
ylbagyle Sednentalion pat
TE KBC d_allaved to stand an anb-C24ulatan
Supenata0t
2SR_3_defined_as MMOF t lear lasMA Fameda
ho top ot yeztial ColuM i0 a Aour t isrecnal
oF SuSPenSiOn s tely
2ESR-_tmm/hauy ales 3-12 mm/hrin 1MAle)
SAgluington
2 KBCCoAtains spea ati4¬AS heyae erpaieto_
echic 4J4lutiñS, Clungi of RBCako_Pldce_asa_
anbn_anthady ractimhsiS kauA aS_A4glutin4bon
ACTaN OF KBL.
Transgort_of oxygen_an Carondidid_
Maintanence_of_aGd bae_halane 10Aic_balanO an VOity
of blae
3) fomLuon of le_PianentS as a eult _of thcir
ithutio0 u retuloeadotkelial SySten
23. PARUL UNIVERSITY Ig. Na.: 2
FACULTY OF PHARMACY Date
PKAC TLCAL6
LenS
ATM
Bod buse
Revev No
uand Use_af ComPaund MicoScope
REQ DLREMEL)
Cosefrausing
MechonisM
objecove Dompauwd icroscope
REFEKENCC
ARM Ra and Rk hoYal NM_Pabel_ PratalAnatamy and
PhySialady, éducaba0Pull&ker_16Edhon
PYNoL B.S SAah_Prakashan_
hdubing
fiN fcsing
Mecnis ARS oFE CoMPOUND MICRaSCOPE
SStage
Base 2It COnsist oF hree Parts Ko sEand, te body
aN_bO rain Of 0ptica lenses
CoMPauNd MICKOSCOPE Á
A Suggor System
12 Base Tts a keavy_meballkc-shaped base_uhich
Supports fke_MiceSope_on_tÁe uwok tasle to_prev
Maximu Stalliy
Am o Cune hanwel_diih_Poieuts_bese_uhich
Sueportso FocusiN_a Mauhiuný SipttM
27. PARUL UNIVERsITY
FACULTY OF PHARMACY
Date
TRACLICA
SLmatian of impahhn CoNtEMt Of 2uh blad
REQUIREMEMES
2Sahlils haemaglahtoMeter_or hemometr which nsE 1
raduaR dlutin4 buleA thin 4ass ad a/ MALJEHE
OP Z culc_lmeter Lcmm_CaLadty_lh_LERz
Nlo Hcldstled unter 7a/ dok Dr 1d
ahyrhent Cotton
RE
FEREIMCES
aaual Rk_and DrPatelNM Practicalanatamy And
ste44 Lth_edtiol_lopi-20la
8.S Shah Prakahan,Ahnmedalad a a12-22
THEORy
a)lon_hload is Mxed_with MloHed RRC_ae haemalysel
an HB5 Rrzted 5 HB Cal/erted nto acid hehatn
wWhich 5esh_brauA in Calr
7he soSLilutel_wth_dstilled aattr tilt patáa wth
o_Stascard 4hss tule 7 HB/ Can dedly je fea
Tom R_qläduated tuse
32. PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig. Ne.:31
Date
RACLICA-4
ALM
Detexnnaidn o clading hing_of aun_hlnad
REQVIREMENT)
DCapillayy_or sldls Cotton, to alahal_priklnylaecdle
KEfEREACEE2
Practical Aatans_anPhusidlagu RLhyal,
DrALMpatel,B.Shan_rakdshahithpdtion
PNCIPLL 22
Dunna_Loaaulaiol Sol faM_ef_llaad is chapgsd to
4 FEtm fK Lime Clapsed, hetueen_te matNtoE
LScape_pÉ blood autside_the_ese And t aksenahion2
o Phusial_change_ taken al clothina i
ne ehueen Alaod escape an eSsel_and fomation
C ibn is Called_clotbina bimg 1 4-lamA
I Cpillay CoAbacksyaAd PlateleEL_Pla4 A0
npay tant e in (eSsatibn OF Lleedn4_
9h0_dstuwlance_in_clotkingtiate may lead toVaA
dislase
33. Pg. No.: 32
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Date
OHaemo Philia 1EALh_ConditionCaused bs aksenc
o any factor reguirid Fe Clatting ie prolatgaienL
clsttn4 Lime_
Puguya S oNLitn assciated wih_platelet
deficiency AaPpensARD blood leakS om_lace vessels
Mrchanism_ot Coaquuhion-
O fomatian_of _thmmboplastin ken bl2ad Comes in
CoAtALtwith KOuah _Sufce_or_isue etact_plateleks_
disiAteAmtes andKumbaplastin is_q919ated_
fonaian oE themkin Te bhramboplasbn ntevacds_i_
plasna prothznmbin and thxanlkin_is_Fomed
foLmaban_of clot Thomkinfemed Convevts_Fhnagen
PRQCEDVAE
Te lipoE invey EipAer sherized_withZal alcahal
Aa sold_pick isaienSo that_bloadFlous Freely ze
StopuatA'Mnedialpty _stavte
e orl_of blood aeaimtely SMM_didneley is elaee
Asyecial_alass_ slile_Tho Ske_is held Veyically eveydoi_
Nobe the
Seen 1A_4y clottinq iML.
me uwhen no _change inshape ofdu
35. Pg. No.: 34
PARUL UNIVERSITTY
FACULTY OF PHARMACY Date
TPRACI TCA-O
ATME
Deteminain_of heart-rate_A pulse-ratt.
REFEKENCE
Prar tiuu AAatams_and Pbusinlo4uLRL4pyal Dr
ApatelL BSShas _piakhan/ ed-2d -SZ
REQDIKEMENI
Awrist WAtch wih secands alM
HEORY
hot at.eSt rate f cnaction andelaxahun
2Ae 2ate h llau_af_bload frem the apila
which Can ay deteliad Wh_Din4EOn a
eart at£ is e Aumke df tne tke hea_
DEAtS w 1bin a_ce tain_enad_cF tMe Ako h/ ate
Can ie_RIt at he wrt Jidk_dE Pk, hadk eÉKARI
37. P A R U L UNIVERSITY
FACULTY OFPHARMACY
Ig. No.: 36
Date
VER
TPRACLICA-1/
To dekemind bload ressuNe_of_Sulied
E a r i E R M E T
P r a c h i u a l A N a t a n u _ a n u s i a l 4 y , r
R.khoyal Dr Natel
BSShah pakasiah Ana 2ol3-/4,An:4S-49_
REQIREIMEAUTS
2Stethascop and
1ezy>PAygmenaiIO.
THEORY
Pice
auscukatiol-1 LDASiIE_OE_
Lax hame CoASist_of tu_Mehallic LulsjonGd,Ly_
a lat CUNg Shfwhich_as a5 a 5ph to Pu
t etkerh ueperends_oF_ule_Eachte ha
per AenzunHl_Lmb which zuns medially doun_uarad
aN_EDWAK aNiM a_LUNature SULtakP
ted in tho_pktenal auetays meatul.
-Spudascj
2)
COnductng tahe They alo SimpleFlexhle_an¢foz
NSU kues_of_Julor_ar late mat9iul. hele tu
OANPCIS_ twCAst_piece eth the PaME_
38. Ig. Na.: 37
P A R U L UNIVERSITY
FACULTY OF PHARMACY Date :
3Cot Piece Zt OASLA OFMekallic tanlayr alat
cn iA diameter And hav4 ai qankeDE SAM-am
in degth 7k dAa4I1_Lhich_1SMate_uP of ellyase
aneliatian f SDundSulhich _are héaLMuCh buder
SPAuAnMmanMeter 1E3_Ao astunent ued_to Measue
Weun AogpessuH DE huma being_tconsitoF_
Moroier Meyry meter COSitOr tue lns ne_lalls _
loAA_
leAnduat lam0at th0_baie_o 2Saat
Sor_oE
atnasghind at
Vale hichNevenESpiln4_oF mezuy 7heari
liMis
Valye which allou ree Comuni.ahon_ q ul
Covekd lith anAtght lid lahtanjg_a
Kupiey bag wth lmin cuFEIi Co/ANr yaLnen
Cut is`4It_and elabc ndi htta wts twe pSA.
zalber ulng okokuhe &tt aith_MetdlAdafterad
othor_with air PUMP_
u Shart_
ke_atke Side_which _Puvided lwha V
VAlve
ThO linea Cuf aS a loud
ctensin 'on OL Side_and al tayna_
SpAug mon'anon eer
Metallic hoak.
39. PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 3P
Date :
ler pMp_wth ualE TiSaSmple_laic ualle
rller u AE OA_AVal ShALe ng a 'si2e that i
iAh b1t_n tuld DpeAIAq WhiCh al_Ftta
values
PKINCILE
The locd Elaw thhaygh a layae S20d arte s_dkstuctal
an pped_Cuouid Ehg la 7_rtSSHN Aslawly
réease
ouctiLA iS studkd o hasii OF_FeelngaF_2ulse_
tseriation d osdlatinn oE My level_Iedhg suith
Stethacepe_tho
tyfey 2ul to_dstuhon
aAd tho attnj At oE lacd thnuah
Sounes prdued hthe_ieametaF_the
Te bload Flou stogs wlea te_preSSure_baANALLKd h
boartey_bbuah 'e_huber "au equal_tag
MAN teLA thO_ble LressL_e_EK e of Iod
through an dlstuchen nicates th_loopessuve
40. PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig No.: 39
Date
POCEDIRE
TN suiect ulas asked to Sit oyhe doun_at eaeon
a CauchiA the SMPINe pItion_Ae Aamests tnud te
amy dye_emeyed'Ho an_wAl laa natabk and
E MsiLiOD wA_adisted Such that_it_wa at to
level oE A@at._
Tho cufF_way_biel aund_Ge aun It should le
neither top tignt to Cause_aAy Ccomfort totho subiect
ADr toa loose to allou its_idvenent_ouA HO am
Te _artey ulas felt _andits CAUBe was matke inHe
(ua_o`a.
2 Mapemeter _uwas placal y bhe side o e Sulyed
etuweenbis An_adbho_bug w OCAL_aftAO Julle
Punp wa tightened u k_Punlg wa_esedko
ate _te haq
whgn Lo_pulse_aear E AaLa 10ted _that_Mek
starts asillating_Ne DxtassillatiaA levela/e sita
NEas/A4 JMA4niteeAA then laullydninn Ze
EVelet_whick E0_osalavos iE i3 kncwNa
Dcillatgy Metha
Auscula toJy_mehed,dFter_ atlatenasuuz)
LchiaaLtoy ia th culital_fosY Ad cAFANA
Saly lkasa4 tA_peeZ lad al
42. PARUL UNIVERSITY
FACULTY OF PHARMACY
Ig. No.: 4I
Date:
UL UNIVVE
PRACL1CAIZZ
Delanination_oF bladAraup
REFEKENCE
Prachal AnAtm_ Andphusialaay r 4eualDrM
pabe BSshdh fprakhshaln 1shedilldA2:8F-Lpana-2a
REQDIREMEAL)
Alicnxapic slid6 4lass mathin4_pencL dnhstm-AAiua2
and Antisen-D Pnckin_ nèeale, to alohal and michce
PKTNCTPIEE
AKonin4 to_ABO_43ten_AE blood_a/2lfiA4 tereCan
eFou blaedas A BAA hdaU
Te_Ani-Sed A_COnSist_aE agalutnin_alpha_aAd Lt
CaS clumging dE RBC AE HOAdCntanq 44914tg
A
AntiSeaSera B_COASAE_oF aAglutiain_jeta anclut
Lauses_ dumeing_o BC _E Lad coAtainna aglutuge.
B_tt_Can'_aktained asfola/-
43. ant..
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 42
Date
Ant:SeM A B
DCRHD
Dduning
in_AlL-R_ADload Araup A
cluaphg
2clungingin_ab-SenB Rloadap A_
clungina iN_Anb-SenA,B> Bload ahupAR
U
No-clunying Clo_cluMgina_
inany Sem2 Bloczd 4aypO
Adbing anti-sta A foms ClumpngaLonina hKE"System
TE RH foMS_cluMPIng_lood 4p> te
Aing anbi-sera B No Clunping
I RH doesnt fom clunpn loe2graup--Ve.
Aving anti-5e oms dunypna.
PROCEDURE)
DTake hree_slidesand mark theM ARadD_
1)Place ypeAseuM_on [5t_dat oF blaod
upe- an secod hp_ot_Dland 1ype-on
Ehi drdp_aE blod
32Steane_lefgne that_ae_prk tAe Finge_and fut_
axp4F load_en slie
Mix it wth e matchStick.
SAlldul t For SMiA_
ramiAL_afLer Smin Clunspp4.
45. PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg.Ne.: 44
Date:
VERS
PRATI CALB
ALM-)
To cany alt He eyEancyle_Sedmentation_rate
REFERENCE
Pracical Anatoms _and Physidlegy,DzRkhpyalL La_
Palel BSShakpakashanKE edEiA 20 4
REQNTREMENT
Westeynenul 0.1MSazuM_Ctnte
Pranciple
T SR_istho_ate at_which ec_loa_cels
SPneAt AaRhdd
Aenatalo44 test Ang a AASPeCiFL_Méasure of
AFlanatidr Mazmally Yed blad celSSettleAlatvely slau
fateY thanYlbmal Yate May0dLate AAnnatin
T PahLAt_ESR_ ishigh it mau_lehte to iannidi
kdney diease_
UHeart degse aoumatau_arthins
46. PARUNIVERSITY
FACULTY OF PHARMACY
Pg. No.: S
Date:
onpbimesto 2SKan leSlaulez_than_Mimal
ASlauw LK_MAy_ALLAte_A aad diiavdr_SuCh_as
PoluCytAnia_SihLL CPN AMnialeukecqtasis
ST nt4_ramei
i)_:22naL_Foz M and
0-29mm farwamP
As the ythrogyte Sf/MeAL_IA_a_pPhiod_efone_
stage st lomiA
1t iniual 0 entatina iso-
unng whih
initial_penidd of a44regationn
kAuleaur Are ome a SrMentatian ilou_
Stag 2-Mex 0 Mu
Isa Periada fastSetlnySedmentaten
OCCUYa ' CoAstant ratLe_duzaa I_pPhdz
stage3
ASentLaion_g4ain slau_a
aib_JAal_jQzizd
packia4 D celsat te bal~un_aE tie thle_
47. P A R U L UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 46
Date :
L L K O C E D I U R E
Wihdau LmlF VevausJlad
Then mixed eract lo M_ Sodium_Gbate ulith Lal
aF VAACus bloodin 4_tule
DInderbad te_Euje 23 2Mes to mixed Ke_lood
thkoughly wth tnt-Loa4ulant
Fl LkO WAleqen tule up to mabiy and
place iA at_E_0o teMPeLzture_M awAy
hom Sunliaht
lake e_eudngs _eCactly_afterjAy Katord io
mihibe_em k Saface_dE Colum to ZpaF
KESDLL-
AO ythincyte_5ed
SedmeAtation_Yate i) ound_Zo
49. Vibron
PARUL UNIVERSITY
FACULTY OF PHARMACY
Pg. Na.: 49
Date :
are tuld CAULtUAS=ontalSinwe
e CaenalS4tUK PntalEfaietl
JamhaidalSutueCoonalsuturei oCalAanc
SauamaS SutuN /¬mf0lalt_pahctal
D Mailla S20 df head Len
fallewo h ah twe allenh han_and seft-
mala T OWaal a tua_Ahal bon
CAONChAe
Olacial- pcatee i ye LhKh sejkte)_ujan
A L4S mal holein_(OnAr -(
e aw ALA Mandhle -O
Facial ond
O Ai pe in aoth uhih nly nl
50. brant
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Date:
kll
all al consist_d 2ar_bad-
Malleus
Mlle Stapes MuS C)
Stape C) -Sma/le t_ an
Awloy asicdej
RIRS TAe_dhe total z4 rib f Stemun
wN 17_L_kAwAal_tL hls_because
ii_dzedlyattach t0 Stenum
la_al_ kaau as falße nis hetale
s Aoiectly attach fo Stezum
Stenun ale_kntun aS ilaatingzls betaue
they ale Flaating nat attacK dh sten
2h /ertebral_coloumn
Consist otal C2)_one
O Cervic
ConSAA OF tota '-bon0
PeSLAEeA lhe_Wee_
aAd dhene abe 2'Ehdkaisi _jcl
heloy_CenicaCobu_L
54. e V i b r a n t .
P A R U L UNIVERSITY
FACULTY OF PHARMACY
Pg. Me. 53
Date :
llna i Medialong_sf to _foreayn anad
loA4PrLAan 1A0radius 1 (onsst_ shot
loey iHenLtie eplr end OF Uha is
stznA aAd Ak lKe StutiuUR_wih lauge
Pevis
Hh7Jot
Shage
ilt_andond Wi Gongt dE Cagal
Bones dE
bnnes7s_a requlafShapAd_bones_a/ arhulate)
Cnur with_ne a n d i r
hC ans OF_alalS Scagholunate tinbal,
-Pakla _Pfpm, bapl20idCaptate_an
aAZ hamate MEtaapal
bIne are iA_D0Ne_ Pun len_aiulae
with istalkou_ 2 Capel_hano_A ths_is
Etahlah etter Facliiy_oE the p jaint wkh__
Troala
Fibaa
Tavsa
Mererasa
phoilanye
h Lhia_ard bula= uaA Tlula o the
l resemAles adiul an_ulna of dhl_oH -ap_
he 1ower end Zilia is the
Sma malile dsC
iO fomKa knet CapP-
DONe ot foot_lere ave_SeVen_baal bonesStuattyD-
l ous and ulp tohearLho weight E TA_04p
ve melalhaal_hane laW y L Phalang
e Anl2 Fr areat_toe A Ach_Eodt _