The document summarizes an experiment studying the effects of doxorubicin, a chemotherapy drug, on the expression of prognostic biomarkers in two breast cancer cell lines, MDAMB- 231 and BT-474. The cells were treated with doxorubicin at the determined IC50 concentration for 48 hours. Total RNA was extracted and real-time PCR was used to analyze expression levels of various genes representing different molecular pathways. The results showed varying expression between the two cell lines, with several markers like L, ZN, ZF and AT having the highest expression levels in the BT-474 cell line. The presence of markers such as P-cadherin, Nrf2 and CD44 suggest the cells still exhibited properties of proliferation

1. Chinmay Menon
28th
August 2015
Effect of chemotherapy drugs on expression of various prognostic
biomarkers using Breast Cancer Cell lines
Abstract:
The objective of this experiment was to study the effects of doxorubicin short-term treatment on
the expression of biomarkers in two breast cancer cell lines namely MDAMB- 231 and BT-474.
Cytotoxicity assay was carried out to determine the IC50 value of doxorubicin in these cell lines.
Drug treatment was given for 48 h with IC50 concentration of doxorubicin with 10% DMEM for
both cell lines. Total RNA was extracted from the remaining viable cells after the drug treatment
for gene expression profiling of markers representing different molecular pathways. Expression
levels of these genes were done by real time PCR and relative gene expression calculated by
normalizing the expression with that of untreated controls cells. The results have shown varying
expression of the markers between the two cell lines. L, ZN, ZF and AT have been found to have
the highest levels of expression, mostly in the BT-474 cell line. The presence of markers such as
P-cadherin, Nrf2 and CD44 suggest that even after drug treatment, the cell is exhibiting
proliferation, invasive and anti-cancer drug properties.