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Dna extraction-principles-1204929342269384-3

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Bhuwan Mathur
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DNA Extraction Principles How do you get DNA out of a whole organism?
How do you get DNA out of a strawberry? Plant Cell Wall: cellulose and protein matrix Cell membrane: lipids and protein Plant Cell Cytoplasm: proteins etc Nucleus: DNA & protein
What does the detergent and NaCl do to the cell parts? Mashing the berry disrupts the cells and membranes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
What does detergent and NaCl do? ,[object Object],[object Object],How do we separate the clumps of cells from the small molecules?
Filtering -Clumps of cellular debris get stuck in the filter. -Small molecules flow thru. How do we get the DNA to separate from the pink liquid?
Precipitate the DNA with cold alcohol ,[object Object],[object Object],[object Object]
Summary ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DNA Properties ,[object Object],[object Object],[object Object],[object Object],[object Object]

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Dna extraction-principles-1204929342269384-3

  • 1. DNA Extraction Principles How do you get DNA out of a whole organism?
  • 2. How do you get DNA out of a strawberry? Plant Cell Wall: cellulose and protein matrix Cell membrane: lipids and protein Plant Cell Cytoplasm: proteins etc Nucleus: DNA & protein
  • 3.
  • 4.
  • 5. Filtering -Clumps of cellular debris get stuck in the filter. -Small molecules flow thru. How do we get the DNA to separate from the pink liquid?
  • 6.
  • 7.
  • 8.