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celiac disease and UTI https://live-biotherapeutic.creative-biolabs.com/urinary-tract-disorders.htm
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mRNA molecule as research tools
Messenger ribonucleic acid (mRNA), a type of RNA molecule that can be found in various cells. In general, the structure of mRNA is often composed of a 5' cap, two untranslated regions (5' UTR and 3' UTR), a coding domain, a 3' poly(A) tail. mRNA can be produced during the process of transcription in the nucleus. Normally, primary transcript mRNA (pre-mRNA) will be created at the early stage of transcription. This pre-mRNA will be further processed into mature mRNA by RNA splicing and removing introns. Mature mRNA can be further transported into the cytoplasm to participate in protein synthesis. Moreover, two main types of RNA molecules, transfer RNA (tRNA) and ribosomal RNA (rRNA), have also been identified that play a critical role in translating codons into amino acids in the protein-manufacturing process.https://mrna.creative-biolabs.com/applications-of-mrna-as-research-tools.htm
Antibody mRNA
Antibodies are immunoglobulins produced by B lymphocytes during their differentiation into plasma cells that bind specifically to the corresponding antigen. Antibodies can be divided into five major classes (IgG, IgA, IgM, IgD, IgE), which have a protective effect on the organism. Antibody mRNAs don't require nuclear localisation to mediate protein translation, but can be translated directly into target proteins or immunogenic activation of the immune response in vivo by using genetic sequence of the virus.https://mrna.creative-biolabs.com/category-antibody-mrna-479.htm
Cytokine mRNA
Cytokines are low molecular weight soluble proteins produced by cells stimulated by immunogens, mitogens or other factors, including interleukins, interferons (IFN), lymphokines, colony-stimulating factors (CSF), chemokines, monoclonal factors and other factors. https://mrna.creative-biolabs.com/category-cytokine-mrna-455.htm
In vitro transcription vector
In vitro transcription (IVT) is an elegant way to obtain highly pure and uniform mRNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides. It is transcribed from a double-stranded DNA template by RNA polymerase (T7, T3 or SP6 RNA polymerase).https://mrna.creative-biolabs.com/category-in-vitro-transcription-vector-452.htm
mRNA products
mRNA products include reagents for mRNA delivery, genome editing mRNA, reporter gene mRNA, growth factor mRNA, mRNA kits, enzymes for mRNA research, and nucleotide products for mRNA research.
https://mrna.creative-biolabs.com/category-mrna-products-443.htm
mRNA Chemical Synthesis
Modified mRNAs can readily be obtained from any DNA template using RNA polymerases in a short time. However, mRNA prepared with enzymatic synthesis may present transcriptional errors, which can only be produced at a laboratory scale, and products may not pure enough for the intended use. An efficient scalable non-enzymatic (chemical) method for mRNA production could solve these problems. https://mrna.creative-biolabs.com/custom-chemical-synthesis-of-mrna.htm
synthetic mRNA
Chemical synthesis of single-stranded mRNA molecules is a widely used laboratory procedure that is an alternative strategy of mRNA synthesis for RNA research as well as RNA biopharmaceuticals. This technique is versatile and allows the researcher to tailor the synthesis of short fragments of mRNA and introduce modifications to mRNA.https://mrna.creative-biolabs.com/custom-chemical-synthesis-of-mrna.htm
iPS Cell Reprogramming by mRNA
Non-integrative mRNA reprogramming has proven to be the most efficient and clinically-relevant method for human induced pluripotent stem cells (iPSCs) reprogramming.
https://mrna.creative-biolabs.com/custom-ips-cell-reprogramming-by-mrna.htm
Recommended
mRNA molecule as research tools
Messenger ribonucleic acid (mRNA), a type of RNA molecule that can be found in various cells. In general, the structure of mRNA is often composed of a 5' cap, two untranslated regions (5' UTR and 3' UTR), a coding domain, a 3' poly(A) tail. mRNA can be produced during the process of transcription in the nucleus. Normally, primary transcript mRNA (pre-mRNA) will be created at the early stage of transcription. This pre-mRNA will be further processed into mature mRNA by RNA splicing and removing introns. Mature mRNA can be further transported into the cytoplasm to participate in protein synthesis. Moreover, two main types of RNA molecules, transfer RNA (tRNA) and ribosomal RNA (rRNA), have also been identified that play a critical role in translating codons into amino acids in the protein-manufacturing process.https://mrna.creative-biolabs.com/applications-of-mrna-as-research-tools.htm
Antibody mRNA
Antibodies are immunoglobulins produced by B lymphocytes during their differentiation into plasma cells that bind specifically to the corresponding antigen. Antibodies can be divided into five major classes (IgG, IgA, IgM, IgD, IgE), which have a protective effect on the organism. Antibody mRNAs don't require nuclear localisation to mediate protein translation, but can be translated directly into target proteins or immunogenic activation of the immune response in vivo by using genetic sequence of the virus.https://mrna.creative-biolabs.com/category-antibody-mrna-479.htm
Cytokine mRNA
Cytokines are low molecular weight soluble proteins produced by cells stimulated by immunogens, mitogens or other factors, including interleukins, interferons (IFN), lymphokines, colony-stimulating factors (CSF), chemokines, monoclonal factors and other factors. https://mrna.creative-biolabs.com/category-cytokine-mrna-455.htm
In vitro transcription vector
In vitro transcription (IVT) is an elegant way to obtain highly pure and uniform mRNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides. It is transcribed from a double-stranded DNA template by RNA polymerase (T7, T3 or SP6 RNA polymerase).https://mrna.creative-biolabs.com/category-in-vitro-transcription-vector-452.htm
mRNA products
mRNA products include reagents for mRNA delivery, genome editing mRNA, reporter gene mRNA, growth factor mRNA, mRNA kits, enzymes for mRNA research, and nucleotide products for mRNA research.
https://mrna.creative-biolabs.com/category-mrna-products-443.htm
mRNA Chemical Synthesis
Modified mRNAs can readily be obtained from any DNA template using RNA polymerases in a short time. However, mRNA prepared with enzymatic synthesis may present transcriptional errors, which can only be produced at a laboratory scale, and products may not pure enough for the intended use. An efficient scalable non-enzymatic (chemical) method for mRNA production could solve these problems. https://mrna.creative-biolabs.com/custom-chemical-synthesis-of-mrna.htm
synthetic mRNA
Chemical synthesis of single-stranded mRNA molecules is a widely used laboratory procedure that is an alternative strategy of mRNA synthesis for RNA research as well as RNA biopharmaceuticals. This technique is versatile and allows the researcher to tailor the synthesis of short fragments of mRNA and introduce modifications to mRNA.https://mrna.creative-biolabs.com/custom-chemical-synthesis-of-mrna.htm
iPS Cell Reprogramming by mRNA
Non-integrative mRNA reprogramming has proven to be the most efficient and clinically-relevant method for human induced pluripotent stem cells (iPSCs) reprogramming.
https://mrna.creative-biolabs.com/custom-ips-cell-reprogramming-by-mrna.htm
hepatitis d vaccines
Hepatitis D (hepatitis delta) is a disease caused by the Hepatitis D Virus (HDV), a small spherical enveloped virusoid.https://www.creative-biolabs.com/vaccine/hepatitis-d-virus-vaccines.htm
hbv vaccines
Hepatitis is a general term for liver inflammation, caused by infectious or non-infectious factors. Hepatitis B virus (HBV) infection is the main cause of morbidity and mortality.https://www.creative-biolabs.com/vaccine/hbv-vaccines.htm
guillain barre syndrome vaccines
Guillain-Barré syndrome (GBS) is an autoimmune disease of the nervous system, which is accompanied by symmetric flaccid paralysis of the extremities, sensory abnormalities, and cranial nerve palsy.https://www.creative-biolabs.com/vaccine/vaccines-for-guillain-barre-syndrome.htm
fiv vaccines
The FIV genome is comprised of three main structural genes, gag, pol, and env, which encode internal structural proteins, viral enzymes and envelope glycoproteins, respectively. https://www.creative-biolabs.com/vaccine/feline-immunodeficiency-virus-vaccine.htm
feline leukemia vaccines
Feline leukemia virus (FeLV) is retroviral in the family Oncovirinae and is one of the most feared causes of a highly contagious and potentially fatal retroviral infection that weakens a cat's immune system.https://www.creative-biolabs.com/vaccine/feline-leukemia-virus-vaccine.htm
egg vaccines
Chicken-egg-based vaccine production remains the most effective manufacturing method available.
cow vaccines
Bovine coronaviruses (BCoVs) belong to the Coronaviridae family in the Nidovirales order which cause respiratory and enteric infections in cattle and wild ruminants. https://www.creative-biolabs.com/vaccine/bovine-coronavirus-vaccines.htm
cmv vaccines
Cytomegalovirus (CMV) is from the family of Herpesviridae. Humans and monkeys serve as natural hosts.https://www.creative-biolabs.com/vaccine/cytomegalovirus-vaccines.htm
glycolipid synthesis.pdf
Due to their surfactants or gelation properties, glycolipids can be used to encapsulate some drugs. Studies have shown that the sucrose esters ability to form various formulation systems for the loading of different active molecules. These results revealed that micro-particles can be obtained with sucrose esters and they exhibit efficient encapsulation properties for pharmaceuticals applications. Several other studies describe the preparation of hydrogels or solution stabilized by sucrose esters for drug dermal absorptions. Therefore, the glycolipid potential is huge in the pharmaceutical and biomedical field.https://www.creative-biolabs.com/glycoprotein/custom-glycolipid-synthesis.htm
D-Galactosamine pentaacetate
D-Galactosamine pentaacetate
Dried by centrifugal evaporation from an aqueous solution.
https://www.creative-biolabs.com/glycoprotein/d-galactosamine-pentaacetate-20.htm
Galactoarabinan.pdf
Dried by centrifugal evaporation from an aqueous solution.
https://www.creative-biolabs.com/glycoprotein/galactoarabinan-202.htm
custom synthesis services.pdf
Membrane carbohydrates, found on the extracellular surface, are complex and can display considerable variability in composition and structure. They are attached with embedded lipids forming glycolipids. Glycolipids constitute a complex family of amphipathic molecules structurally characterized by a hydrophilic mono- or oligo-saccharide moiety linked to a hydrophobic ceramide moiety.https://www.creative-biolabs.com/glycoprotein/custom-glycolipid-synthesis.htm
Anti-Glycoprotein Antibody Development.pdf
Phage Display Technology for Anti-Glycoprotein mAbs Discovery
Hybridoma Technology for Anti-Glycoprotein mAbs Discovery
Antigen-Specific B Lymphocytes Sorting Technology for Anti-Glycoprotein mAbs Discovery
https://www.creative-biolabs.com/glycoprotein/anti-glycoprotein-antibody-development.htm
Glycosylation Site Mapping.pdf
Glycosylation site mapping helps the identification of a glycosylation site, which can provide an indication of the function of that glycan. In addition, site-specific glycosylation analysis and assignment of a certain structure to a specific site can illuminate the protein’s glycosylation profile, microheterogeneity, and its activity. Glycosylation has great importance in the development of biological drugs because their glycan chains markedly affect product stability, activity, antigenicity, and pharmacodynamics. Glycosylation site mapping mainly contributes to glycan microheterogeneity.
https://www.creative-biolabs.com/glycoprotein/glycosylation-site-mapping.htm
Glycoprotein-based Vaccine Development.pdf
Anticancer immunotherapy has emerged as a new exciting area for controlling tumors. Particularly, vaccination using synthetic tumor-associated carbohydrate antigens (TACAs)-based vaccine hold promise for generating a specific antitumor response by targeting cancer cells.https://www.creative-biolabs.com/glycoprotein/glycopeptide-based-cancer-vaccine-development.htm
Glycoprotein Structure Analysis.pdf
Glycosylation is a fundamental modification of proteins, which plays an important role in many physiological and pathological processes, including protein folding and trafficking, cell-cell and cell-matrix interaction, cellular differentiation, fertilization, immune response, the initiation and metastasis of tumors. Thus, the analysis of glycoproteins structure allows characterization of important biological molecules, including antibodies, vaccines, enzymes, etc. What's more, glycoprotein structure analysis would be of great diagnostic and clinical importance.https://www.creative-biolabs.com/glycoprotein/glycoprotein-structure-analysis.htm
Glycoprotein Detection.pdf
Glycoprotein detection is an important part of glycosylation engineering. https://www.creative-biolabs.com/glycoprotein/glycoprotein-detection.htm
Glycoprotein Detection by Staining Procedures.pdf
There are a number of staining methods that can be used to detect highly glycosylated proteins on SDS gels, among which, periodic acid-Schiff (PAS) staining is the simplest and low-cost way to estimate whether a protein is glycosylated.
https://www.creative-biolabs.com/glycoprotein/glycoprotein-detection-by-staining-procedures.htm
Glycoprotein detection by PAS staining method.pdf
Glycoproteins are extremely important to a variety of biological processes, including protein folding and trafficking, cell-cell and cell-matrix interaction, cellular differentiation, fertilization, immune response, the initiation and metastasis of tumors. However, the analysis of glycoproteins is a big challenge because of the low abundance, the complexity of the glycan structures, the multiple substitutions (microheterogeneity) at glycosylation sites, and the structural diversity associated with the protein backbone itself. We can provide a series of technologies for glycoprotein detection and analysis.
https://www.creative-biolabs.com/glycoprotein/glycoprotein-detection-by-staining-procedures.htm
Glycoprotein Biomarker.pdf
Glycoproteins are a group of proteins with oligosaccharide chains or glycans covalently attached to amino acid side-chains. These proteins are ubiquitously distributed and play an important role in various biological processes, such as cell signaling, cell-cell interaction, immune recognition, cell proliferation, and differentiation. Therefore, the alterations of glycoproteins possess a close relationship with the progression of diseases, such as autoimmune disease, Alzheimer’s disease, and cancer.
https://www.creative-biolabs.com/glycoprotein/glycoprotein-biomarkers-research.htm
Dandelion Hashtable: beyond billion requests per second on a commodity server
This slide deck presents DLHT, a concurrent in-memory hashtable. Despite efforts to optimize hashtables, that go as far as sacrificing core functionality, state-of-the-art designs still incur multiple memory accesses per request and block request processing in three cases. First, most hashtables block while waiting for data to be retrieved from memory. Second, open-addressing designs, which represent the current state-of-the-art, either cannot free index slots on deletes or must block all requests to do so. Third, index resizes block every request until all objects are copied to the new index. Defying folklore wisdom, DLHT forgoes open-addressing and adopts a fully-featured and memory-aware closed-addressing design based on bounded cache-line-chaining. This design offers lock-free index operations and deletes that free slots instantly, (2) completes most requests with a single memory access, (3) utilizes software prefetching to hide memory latencies, and (4) employs a novel non-blocking and parallel resizing. In a commodity server and a memory-resident workload, DLHT surpasses 1.6B requests per second and provides 3.5x (12x) the throughput of the state-of-the-art closed-addressing (open-addressing) resizable hashtable on Gets (Deletes).
How to Interpret Trends in the Kalyan Rajdhani Mix Chart.pdf
A Mix Chart displays historical data of numbers in a graphical or tabular form. The Kalyan Rajdhani Mix Chart specifically shows the results of a sequence of numbers over different periods.
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hepatitis d vaccines
Hepatitis D (hepatitis delta) is a disease caused by the Hepatitis D Virus (HDV), a small spherical enveloped virusoid.https://www.creative-biolabs.com/vaccine/hepatitis-d-virus-vaccines.htm
hbv vaccines
Hepatitis is a general term for liver inflammation, caused by infectious or non-infectious factors. Hepatitis B virus (HBV) infection is the main cause of morbidity and mortality.https://www.creative-biolabs.com/vaccine/hbv-vaccines.htm
guillain barre syndrome vaccines
Guillain-Barré syndrome (GBS) is an autoimmune disease of the nervous system, which is accompanied by symmetric flaccid paralysis of the extremities, sensory abnormalities, and cranial nerve palsy.https://www.creative-biolabs.com/vaccine/vaccines-for-guillain-barre-syndrome.htm
fiv vaccines
The FIV genome is comprised of three main structural genes, gag, pol, and env, which encode internal structural proteins, viral enzymes and envelope glycoproteins, respectively. https://www.creative-biolabs.com/vaccine/feline-immunodeficiency-virus-vaccine.htm
feline leukemia vaccines
Feline leukemia virus (FeLV) is retroviral in the family Oncovirinae and is one of the most feared causes of a highly contagious and potentially fatal retroviral infection that weakens a cat's immune system.https://www.creative-biolabs.com/vaccine/feline-leukemia-virus-vaccine.htm
egg vaccines
Chicken-egg-based vaccine production remains the most effective manufacturing method available.
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Bovine coronaviruses (BCoVs) belong to the Coronaviridae family in the Nidovirales order which cause respiratory and enteric infections in cattle and wild ruminants. https://www.creative-biolabs.com/vaccine/bovine-coronavirus-vaccines.htm
cmv vaccines
Cytomegalovirus (CMV) is from the family of Herpesviridae. Humans and monkeys serve as natural hosts.https://www.creative-biolabs.com/vaccine/cytomegalovirus-vaccines.htm
glycolipid synthesis.pdf
Due to their surfactants or gelation properties, glycolipids can be used to encapsulate some drugs. Studies have shown that the sucrose esters ability to form various formulation systems for the loading of different active molecules. These results revealed that micro-particles can be obtained with sucrose esters and they exhibit efficient encapsulation properties for pharmaceuticals applications. Several other studies describe the preparation of hydrogels or solution stabilized by sucrose esters for drug dermal absorptions. Therefore, the glycolipid potential is huge in the pharmaceutical and biomedical field.https://www.creative-biolabs.com/glycoprotein/custom-glycolipid-synthesis.htm
D-Galactosamine pentaacetate
D-Galactosamine pentaacetate
Dried by centrifugal evaporation from an aqueous solution.
https://www.creative-biolabs.com/glycoprotein/d-galactosamine-pentaacetate-20.htm
Galactoarabinan.pdf
Dried by centrifugal evaporation from an aqueous solution.
https://www.creative-biolabs.com/glycoprotein/galactoarabinan-202.htm
custom synthesis services.pdf
Membrane carbohydrates, found on the extracellular surface, are complex and can display considerable variability in composition and structure. They are attached with embedded lipids forming glycolipids. Glycolipids constitute a complex family of amphipathic molecules structurally characterized by a hydrophilic mono- or oligo-saccharide moiety linked to a hydrophobic ceramide moiety.https://www.creative-biolabs.com/glycoprotein/custom-glycolipid-synthesis.htm
Anti-Glycoprotein Antibody Development.pdf
Phage Display Technology for Anti-Glycoprotein mAbs Discovery
Hybridoma Technology for Anti-Glycoprotein mAbs Discovery
Antigen-Specific B Lymphocytes Sorting Technology for Anti-Glycoprotein mAbs Discovery
https://www.creative-biolabs.com/glycoprotein/anti-glycoprotein-antibody-development.htm
Glycosylation Site Mapping.pdf
Glycosylation site mapping helps the identification of a glycosylation site, which can provide an indication of the function of that glycan. In addition, site-specific glycosylation analysis and assignment of a certain structure to a specific site can illuminate the protein’s glycosylation profile, microheterogeneity, and its activity. Glycosylation has great importance in the development of biological drugs because their glycan chains markedly affect product stability, activity, antigenicity, and pharmacodynamics. Glycosylation site mapping mainly contributes to glycan microheterogeneity.
https://www.creative-biolabs.com/glycoprotein/glycosylation-site-mapping.htm
Glycoprotein-based Vaccine Development.pdf
Anticancer immunotherapy has emerged as a new exciting area for controlling tumors. Particularly, vaccination using synthetic tumor-associated carbohydrate antigens (TACAs)-based vaccine hold promise for generating a specific antitumor response by targeting cancer cells.https://www.creative-biolabs.com/glycoprotein/glycopeptide-based-cancer-vaccine-development.htm
Glycoprotein Structure Analysis.pdf
Glycosylation is a fundamental modification of proteins, which plays an important role in many physiological and pathological processes, including protein folding and trafficking, cell-cell and cell-matrix interaction, cellular differentiation, fertilization, immune response, the initiation and metastasis of tumors. Thus, the analysis of glycoproteins structure allows characterization of important biological molecules, including antibodies, vaccines, enzymes, etc. What's more, glycoprotein structure analysis would be of great diagnostic and clinical importance.https://www.creative-biolabs.com/glycoprotein/glycoprotein-structure-analysis.htm
Glycoprotein Detection.pdf
Glycoprotein detection is an important part of glycosylation engineering. https://www.creative-biolabs.com/glycoprotein/glycoprotein-detection.htm
Glycoprotein Detection by Staining Procedures.pdf
There are a number of staining methods that can be used to detect highly glycosylated proteins on SDS gels, among which, periodic acid-Schiff (PAS) staining is the simplest and low-cost way to estimate whether a protein is glycosylated.
https://www.creative-biolabs.com/glycoprotein/glycoprotein-detection-by-staining-procedures.htm
Glycoprotein detection by PAS staining method.pdf
Glycoproteins are extremely important to a variety of biological processes, including protein folding and trafficking, cell-cell and cell-matrix interaction, cellular differentiation, fertilization, immune response, the initiation and metastasis of tumors. However, the analysis of glycoproteins is a big challenge because of the low abundance, the complexity of the glycan structures, the multiple substitutions (microheterogeneity) at glycosylation sites, and the structural diversity associated with the protein backbone itself. We can provide a series of technologies for glycoprotein detection and analysis.
https://www.creative-biolabs.com/glycoprotein/glycoprotein-detection-by-staining-procedures.htm
Glycoprotein Biomarker.pdf
Glycoproteins are a group of proteins with oligosaccharide chains or glycans covalently attached to amino acid side-chains. These proteins are ubiquitously distributed and play an important role in various biological processes, such as cell signaling, cell-cell interaction, immune recognition, cell proliferation, and differentiation. Therefore, the alterations of glycoproteins possess a close relationship with the progression of diseases, such as autoimmune disease, Alzheimer’s disease, and cancer.
https://www.creative-biolabs.com/glycoprotein/glycoprotein-biomarkers-research.htm
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This slide deck presents DLHT, a concurrent in-memory hashtable. Despite efforts to optimize hashtables, that go as far as sacrificing core functionality, state-of-the-art designs still incur multiple memory accesses per request and block request processing in three cases. First, most hashtables block while waiting for data to be retrieved from memory. Second, open-addressing designs, which represent the current state-of-the-art, either cannot free index slots on deletes or must block all requests to do so. Third, index resizes block every request until all objects are copied to the new index. Defying folklore wisdom, DLHT forgoes open-addressing and adopts a fully-featured and memory-aware closed-addressing design based on bounded cache-line-chaining. This design offers lock-free index operations and deletes that free slots instantly, (2) completes most requests with a single memory access, (3) utilizes software prefetching to hide memory latencies, and (4) employs a novel non-blocking and parallel resizing. In a commodity server and a memory-resident workload, DLHT surpasses 1.6B requests per second and provides 3.5x (12x) the throughput of the state-of-the-art closed-addressing (open-addressing) resizable hashtable on Gets (Deletes).
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zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...
Folding is a recent technique for building efficient recursive SNARKs. Several elegant folding protocols have been proposed, such as Nova, Supernova, Hypernova, Protostar, and others. However, all of them rely on an additively homomorphic commitment scheme based on discrete log, and are therefore not post-quantum secure. In this work we present LatticeFold, the first lattice-based folding protocol based on the Module SIS problem. This folding protocol naturally leads to an efficient recursive lattice-based SNARK and an efficient PCD scheme. LatticeFold supports folding low-degree relations, such as R1CS, as well as high-degree relations, such as CCS. The key challenge is to construct a secure folding protocol that works with the Ajtai commitment scheme. The difficulty, is ensuring that extracted witnesses are low norm through many rounds of folding. We present a novel technique using the sumcheck protocol to ensure that extracted witnesses are always low norm no matter how many rounds of folding are used. Our evaluation of the final proof system suggests that it is as performant as Hypernova, while providing post-quantum security.
Paper Link: https://eprint.iacr.org/2024/257
Day 2 - Intro to UiPath Studio Fundamentals
In our second session, we shall learn all about the main features and fundamentals of UiPath Studio that enable us to use the building blocks for any automation project.
📕 Detailed agenda:
Variables and Datatypes
Workflow Layouts
Arguments
Control Flows and Loops
Conditional Statements
💻 Extra training through UiPath Academy:
Variables, Constants, and Arguments in Studio
Control Flow in Studio
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