2. Six helix bundle (6HB)
Alexa488 TEG-CH
To consturct a rod-like DNA nanostructure (422nm x 6nm) with
cholesteryl moieties (spacing of 42 nm=12 helical turns) on one
side labeled with fluorofor on the other side
Used to follow initial steps in protein-assisted membrane sculpting
3. DOPC/BSM/Chol 2/2/1 - addition of MgCl2 (final conc. 10mM)
CH-modified 6HB on phase separated vesicles –
„switchable” partitioning to different domains
DiD - marker for disordered membrane domains Origami DNA (6HB) labeled with Alexa 488