Statins Talk 2009

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rhondagarza

Health & Medicine Technology

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R s R S S Fimicute Clostridium sordellii See Fig. 3 and comments. R: Resistant, little or no zone of inhibition; S: susceptible, clear zone of inhibition; s: slightly susceptible, hazy zone of inhibtion S S S S S Fungi Candida albicans R R R R R Enterobacteria E. aerogenes R R R R R Enterobacteria S. marcescens R R s R R Enterobacteria K. pneumoniae R R s s s Fimicute S. epidemidis R R S S S Enterobacteria E. coli R R S S S Fimicute S. aureus (MRSA&MSSA) s S S S S Enterobacteria P. mirabilis R S S S S Fimicute B. subtilis Crestor Lovastatin Lipitor Fluvastatin Zocor Taxonomic group Species Table 1. Variable susceptibilities of bacterial species to individual statins by agar-based assay

Bacillus subtilis Zocor Lovastatin Lovastatin (1:10) Zocor (1:10) Fluvastatin Fluvastatin (1:10)

Clostridium sordellii Zocor Lovastatin Lovastatin (1:10) Zocor (1:10) Fluvastatin Fluvastatin (1:10)

Clostridium sordellii Crestor DMSO Act.Buffer Crestor (1:10) Lipitor Lipitor (1:10)

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Antimicrobial activity of Lactobacillus spp. especially (L. planetarium and L. acidophilus) against S. aureus were tested using agar-plug, agar well diffusion methods to select the best isolate that could inhibit the growth of multidrug resistance isolates. Further identification for the presence of bacteriocin was done using ELISA kit. Results showed that Lactobacillus spp isolates were bacteriocin producers with different degrees and that L. planetarium (L7) was the most efficient in bacteriocin production. Therefore, L. planetarium (L7) was selected for purification using 70% saturated ammonium sulfate and gel chromatography. The effect of purified bacteriocin was tested on 16 bacterial isolates using micro-titer plate method and well diffusion method. The results showed the ability of the bacteriocin to inhibit bacteria only at concentrations 1866U/ml (50%), 3732U/ml (100%) with a diameter of inhibition zones ranges between (11-23 mm) respectively. The anti-biofilm activity of purified bacteriocin at concentration 100% was investigated and the results showed that biofilm formation was reduced by 100% in the presence of bacteriocin.

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