A Pictorial Representation Of What I Am Currently Doing
Upcoming SlideShare
Loading in...5
×
 

A Pictorial Representation Of What I Am Currently Doing

on

  • 184 views

Recent research-Cancer with CPMC, San Francisco

Recent research-Cancer with CPMC, San Francisco

Statistics

Views

Total Views
184
Views on SlideShare
184
Embed Views
0

Actions

Likes
0
Downloads
0
Comments
0

0 Embeds 0

No embeds

Accessibility

Categories

Upload Details

Uploaded via as Microsoft Word

Usage Rights

© All Rights Reserved

Report content

Flagged as inappropriate Flag as inappropriate
Flag as inappropriate

Select your reason for flagging this presentation as inappropriate.

Cancel
  • Full Name Full Name Comment goes here.
    Are you sure you want to
    Your message goes here
    Processing…
Post Comment
Edit your comment

A Pictorial Representation Of What I Am Currently Doing A Pictorial Representation Of What I Am Currently Doing Document Transcript

  • A Pictorial representation of what I am currently doing:MTT assays.Fig 1: Orange color due to addition of MTT reagent to detect cell viability in eachwellFig 2: Initial plating and treatment of mammalian cells in 10% FBS media
  • FACS analysis performed with 6 well plates.Cells per well : 175KMedia: 3 mL in each well2-Day to 3- Day treatments depending on the purposeROS determining agent added on Day 2 of the treatment, prepared fresh.Also done to determine Annexin-PI binding of cells showing usual apoptosis(e.g Annexin binds to the phospholipids, facing outside, of a programmed to diecell and thus this activity can determine apoptosis/necrosis of the treated cells)
  • DRUG PREPARATIONS (High school Chemistry ) DRUGS kept on ice before use DRUG samples (including stocks) in Storage FRESH drug dilutions prepared for treatment View slide