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The Penn Experience with MAGE-TAB John Brestelli Elisabetta Manduchi Junmin Liu Jonathan Schug Chris Stoeckert NCI MAGE Workshop Jan 24, 2008
http://www.cbil.upenn.edu/RAD/
Support diabetes research
Support research on protozoan parasites
MAGE-TAB Studies so far … ,[object Object],[object Object],[object Object],[object Object],[object Object]
Example MAGE-TAB spreadsheet
 
Lessons Learned ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Do MAGE-TAB templates have to follow MAGE-TAB specifications? Of course, but the biologists filling them out don’t need to know that!
MAGE-TAB Tools and Process ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Handling MAGE docs in unified way processors validators translator O/R layer GUS DB mage doc Mage doc  in other formats graph common objects ontology matrix
MR_Ti overview Reader Processor Validator Translator Study->submit Reporter Tester VO’ VO VO GusObj MagemlReader MageTabReader SoftReader BaseProcessor ProcessModule MergeTreatmentSerials NamingBioSamples NamingScans BaseValidator ValidationRule ProtocolHasMageType StudyDesignHasMageType AssayHasFactorValues make ServiceFactroy GUS Commit MR_Tilogger SqlReporter SqlTester Config.xml read-in use use use use use use MR_TiException VoToGusTranslator VoToMageTab VoToDot
MAGE-TAB Tools and Process Initial contact with investigator/ Review publication Provide appropriate  MAGE-TAB template Get filled in template Download SOFT files  from GEO/ Review publication If Affymetrix, use ArrayExpress converter tool to generate MAGE-Tab; if cDNA array, in-house script to generate template . Review tab files and fill in protocols, experimental factors, ontology terms as needed.   Validate, load, and check graph MAGE-ML files From ArrayExpress Convert to MAGE-Tab  using mage2tab Convert to MAGE-Tab  using template2tab
Details ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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RAD: Christian J. Stockert Jr, Junmin Liu

  • 1. The Penn Experience with MAGE-TAB John Brestelli Elisabetta Manduchi Junmin Liu Jonathan Schug Chris Stoeckert NCI MAGE Workshop Jan 24, 2008
  • 4. Support research on protozoan parasites
  • 5.
  • 7.  
  • 8.
  • 9. Do MAGE-TAB templates have to follow MAGE-TAB specifications? Of course, but the biologists filling them out don’t need to know that!
  • 10.
  • 11. Handling MAGE docs in unified way processors validators translator O/R layer GUS DB mage doc Mage doc in other formats graph common objects ontology matrix
  • 12. MR_Ti overview Reader Processor Validator Translator Study->submit Reporter Tester VO’ VO VO GusObj MagemlReader MageTabReader SoftReader BaseProcessor ProcessModule MergeTreatmentSerials NamingBioSamples NamingScans BaseValidator ValidationRule ProtocolHasMageType StudyDesignHasMageType AssayHasFactorValues make ServiceFactroy GUS Commit MR_Tilogger SqlReporter SqlTester Config.xml read-in use use use use use use MR_TiException VoToGusTranslator VoToMageTab VoToDot
  • 13. MAGE-TAB Tools and Process Initial contact with investigator/ Review publication Provide appropriate MAGE-TAB template Get filled in template Download SOFT files from GEO/ Review publication If Affymetrix, use ArrayExpress converter tool to generate MAGE-Tab; if cDNA array, in-house script to generate template . Review tab files and fill in protocols, experimental factors, ontology terms as needed. Validate, load, and check graph MAGE-ML files From ArrayExpress Convert to MAGE-Tab using mage2tab Convert to MAGE-Tab using template2tab
  • 14.