OLIGONUCLEOTIDE (ODN) FLUORESCENCE IN SITU HYBRIDIZATION (OLIGO-FISH) AND CONVENTIONAL FISH ALLOW ENUMERATION OF 24 CHROMOSOMES IN 6 SUCCESSIVE HYBRIDIZATIONS PERFORMED IN A SINGLE DAY.
Joan Aurich-Costa, Carrie Ng, Susan Selvaggio, Pere Colls* and Sean Bradley
One Cell Systems, Inc., Cambridge, MA. *Reprogenetics, LLC, Livingston, NJ.
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OligoFISH 24 chromosome enumeration
1. OligoFISH and conventional FISH allow enumeration of 24 chromosomes in 6 successive hybridizations performed in a single day Joan Aurich-Costa, Carrie Ng, Susan Selvaggio, Pere Colls* and Sean Bradley Cellay, Inc., Cambridge, MA. *Reprogenetics, LLC, Livingston, NJ. Support: NSF grant: IIP-0724876
16. 1st FISH: 3- Gd , 7- A , 12- R , 16- G 2nd FISH: 2- R , 13-21- G , 18- A , 20- Gd 3rd FISH: 6- A , 8- Gd , 9q12- G , 11- R 4th FISH: X- R , Yq12- G , 15- Gd , 17- A 5th FISH: 1q12- A , 4- R , 10- Gd , 14-22- G 6th FISH (BACs): 5- A , 19- Gd , 21- G , 22- R Blastomere A
17. 12 R 16 G 3 Gd 7 A 13-21 G 2 R 20 Gd 18 A 6 A 11 R 8 Gd 9q12 G 17 A 15 Gd X R Yq12 G 14-22 G 10 Gd 1q12 A 4 R 21 G 5 A 22 R 19 Gd 1st FISH: 3- Gd , 7- A , 12- R , 16- G 2nd FISH: 2- R , 13-21- G , 18- A , 20- Gd 3rd FISH: 6- A , 8- Gd , 9q12- G , 11- R 4th FISH: X- R , Yq12- G , 15- Gd , 17- A 5th FISH: 1q12- A , 4- R , 10- Gd , 14-22- G 6th FISH (BACs): 5- A , 19- Gd , 21- G , 22- R Blastomere A
20. Blastomere E (Cytoplasm) 1st FISH: 3- Gd , 7- A , 12- R , 16- G 2nd FISH: 2- R , 13-21- G , 18- A , 20- Gd 3rd FISH: 6- A , 8- Gd , 9q12- G , 11- R 4th FISH: X- R , Yq12- G , 15- Gd , 17- A 5th FISH: 1q12- A , 4- R , 10- Gd , 14-22- G 6th FISH (BACs): 5- A , 19- Gd , 21- G , 22- R
23. The FISH Team Production Team: Lauren King, BS Brian Kennedy Dave Church Robert Trager Production Manager: Carrie Ng, BS R&D: Sean Bradley, PhD Director: Joan Aurich, PhD