1. RBCPolymerases
*
Store at
-200C
2X RBC Taq DNA Polymerase Mastermix
Master Mixes standardise your results
Cat.No. RT006
V2.0
RBC Taq DNA Polymerase Mastermix
Cat.No.RT006
2X RBC Taq Mastermix: 500μl x 5vials (100rxn)
50mM MgCI2: 1ml x 1vial
Description
RBC Taq DNA Polymerase Mastermix is a ready-to-use mastermix. Convenient for amplification of DNA fragments without the
need to thaw individual components, reducing the risk of contamination and pipetting errors. RBC Taq DNA Polymerase
Mastermix utilises a Mg2+ base reaction buffer system with high quality RBC Taq DNA polymerase, containing all the nucleotides
and reagents necessary to perform a standard reaction.
Storage Buffer
RBC Taq DNA Polymerase Mastermix must be stored at either -20˚C or 4˚C. Storage at 4˚C avoids the necessity of thawing the mix
before assembling the reaction. For long term storage, -20˚C is recommended.
Component
Each vial contains 500μl of a 2X working concentration master mix. The addition of template and primers results in a 50ul final
reaction volume, containing
10 mM KCl, 1.5mM MgCl2, 20 mM Tris-HCl (pH8.8), 10 mM (NH4)2SO4, 0.1 mg/ml BSA, 0.2 mM dNTP mix, 1.25 U RBC Taq DNA
polymerase.
User may aliquot into microtubes according to desired reaction volume or according to recommendations below.
Recombinant
5’ to 3’ Exonuclease
3’ to 5’ Exonuclease
Terminal dA Addition
Endonuclease Free
2. General Reaction Conditions
The following protocol is suggested as a starting point and guideline when using RBC Taq DNA Polymerase
Mastermix. We recommend assembling reactions on ice from pre-chilled components. This protocol is for a
reaction size of approximately 50 μl. The reaction size may be adjusted as desired.
1
Set up reaction tubes / plates on ice.
2
Add the following components in any order to each reaction vessel.
* 25μl for RBC Taq DNA Polymerase Mastermix
* Primers (200 nM final concentration per primer is recommended)
* Template DNA solution
3 Mix contents and run amplification program. Utilise standard amplification parameters for amplification of
vast majority of DNA sequences. User may also optimize for specific gene sequences.
RBCPolymerases Product Information
Cat.No.
Recommended product
Specification
RT001/RTT01
RT011/RTT11
RT003
RT033
RT004
RT006
RT007/RT0071
RT008
RT0081
RT0082
RT0083
RT009
RT010
RBC Taq DNA Polymerase with dNTPs
RBC Taq DNA Polymerase
RBC Hi DNA Polymerase with dNTPs
RBC Hi DNA Polymerase
RBC Pfu DNA Polymerase
2X RBC Taq DNA Polymerase Mastermix
2X Blue/Red Mix DNA Polymerase Mastermix
RBC SensiZyme Hotstart Taq Premix
RBC SensiZyme Hotstart Taq with dNTPs
RBC SensiZyme Hotstart Taq
RBC SensiZyme Hotstart Taq Bluemix
RBC ThermOne Real-Time Premix (SYBR Green)
RBC ThermOne Real-Time Premix (Probe Method)
RBC Taq DNA Polymerase, 10X Reaction Buffer, 10mM dNTPs Mix
RBC Taq DNA Polymerase, 10X Reaction Buffer
RBC Hi DNA Polymerase, 10X Reaction Buffer, 10mM dNTPs Mix
RBC Hi DNA Polymerase, 10X Reaction Buffer
RBC Pfu DNA Polymerase, 10X Reaction Buffer
2X RBC Taq Mastermix, 50mM MgCl2
2X RBC Taq Bluemix / Redmix
2X SensiZyme Hotstart Taq Premix, 50mM MgCl2
SensiZyme Hotstart Taq, 10X SensiZyme Buffer, 10mM dNTPs Mix
SensiZyme Hotstart Taq, 10X SensiZyme Buffer
2X SensiZyme Hotstart Taq Bluemix
1ml X 5vials
1ml X 5vials
www.rbcbioscience.com
