Quantitative Real Time PCR: A fast and accurate method for identification of virulence factors in swine.
Find in this presentation a more sensitive and specific alternative for microbiological isolation and conventional PCR.
Get more info in: http://www.exopol.com/en/qpcr
2. EXOone Virulence Factors E. coli oneMIX
Available kits
Fimbriae: F4, F5, F6, F18, F41
Toxins: LT, STa, STb, STx2e
Endogenous control: ECCO
Suitable diagnosis
Oedemas disease
Neonatal colibacillosis
Postweaning diarrhea
Run every assay simultaneously with same thermal profile.
Generate all the info in just 2 hours!
3. Work with pools of rectal
swabs, feces or intestines
As the qPCR is able to quantify the desired target,
relative interpretation might be done in order to figure
out Virulence Factors of the majority population of E.
coli.
Why settling with the info from just few selected colonies?
Microbiological culture of each sample and selection and purification of
colonies is tedious and might miss important information.
Testing directly the clinical samples allow further information in considerably
less time.
EXOone Virulence Factors E. coli oneMIX