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The Potential for Isotope and Chemical Markers to Differentiate between Sources of Nitrate Contamination in Surface Waters. Monday, 28 th  March, 2011
What does this mean?
[object Object],[object Object],[object Object],Background
Nitrate is all around us
Importance of NSD Methemoglobinemia Cancer EU WHO Polluter Pays Principle Effective Remediation Eutrophication Fish Kills
Ways to Achieve NSD
[object Object],[object Object],State of Knowledge ,[object Object]
What are Isotopes? ,[object Object],[object Object],[object Object],[object Object],[object Object]
Stable Isotopes & Nitrate Heavy Isotope Light  Isotope 15 N   14 N 18 O   17 O   16 O
N Isotopic Fractionation (Townsend 2008)
N Isotopic Fractionation (Townsend 2008)
N Isotopic Fractionation (Townsend 2008) Preferential loss of  14 N as gas. Residual nitrate  ↑  15 N Nitrate Denitrification Nitrogen Gases
N Isotopic Fractionation (Townsend 2008)
Isotopic Measurements ,[object Object],[object Object],where: R = heavy isotope : light isotope
The Dual Isotope Approach (Kendall 1998)
Manure & Septic Issues ,[object Object],(Kendall 1998)
[object Object],The Project ,[object Object],[object Object]
Use of Chemical Markers
Project Plan A multi-residue SPE-LC-MS-MS Method Database of isotope and chemical markers in Ireland Model for nitrate source differentiation
Conclusions ,[object Object],[object Object],[object Object]
Conclusions ,[object Object],[object Object],[object Object]
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