18. • Aseptic conditions while instilling drugs.
• Do not use the solution, if it is discolored (check the expiry
date of given drug).
• Experiment should be performed in dim lighted room.
• Check for any eye disease such as conjunctivitis, uveitis, etc.
• Remove the eyelashes carefully.
• Rabbit needs to be gently handled.
• Central part of cornea should not be touched.
Precautions:
20. STEP1
• Weigh and mark the rabbits. Select
3 rabbits (one for pupil reflex,
second for corneal reflex and third
for the light reflex)
• The alternate eye of a rabbit works
as control and test group (to avoid
individual variation).
21. STEP2:
• Restrain the rabbit in the suitable
restrainer and fix it for 5 mins (to
accomodate and acclimitize rabbit
into the restrainer).
• Select the control and test eye
according to your preference (right
or left), and mark it with test eye
(TE) for test and control eye (CE)
for control.
• Eye lashes of both eyes is trimmed
out with scissor and normal saline
is put in both eyes to make them
clean and clear.
22. STEP3:
• The drug is instilled* with the help
of dropper in test eye (not more
than 2-3 drops).
• Then, the response is noted and
both test and control eyes are
compared.
*Volume to be instilled in rabbit eye
sac should be 40-60
29. USE OF
MYDRIATICS
• For testing, refractive
error, fundoscopy and
retinoscopy.
• Prevent pain in iritis,
iridocyclitis and
choroiditis.
• To prevent adhesion/
synechiae formation in
uveitis.
30. USE OF MIOTICS
• Cataract extraction and
other surgical procedure
in anterior chamber.
• Glaucoma
• To overcome mydriasis
by agents like Atropine.
• To break adhesions by
alternatively given with
Atropine.
