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Lab Notebook
Bio 201 102 Terrence Harrison
Lori Gardner
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Contents
Laboratory Procedures....................................................................................................................2
INTRODUCTION:.............................................................................................................................2
Scientific Method............................................................................................................................3
SCIENTIC METHOD STEPS: ...............................................................................................................3
Investigation paper.........................................................................................................................4
Investigation paper Revised.............................................................................................................7
Light Microscope ..........................................................................................................................12
Light Microscope..............................................................................................................................12
Mitosis.........................................................................................................................................14
Cytology.......................................................................................................................................16
The Cell........................................................................................................................................16
Histology and Integument.............................................................................................................17
Epithelial Tissues...........................................................................................................................19
Connective Tissues........................................................................................................................21
Cartilage.......................................................................................................................................22
Muscle Tissues..............................................................................................................................23
Nervous .......................................................................................................................................23
Skin with Hair Follicle....................................................................................................................24
Integumentary..............................................................................................................................26
Anatomical Position and Planes.....................................................................................................27
Skeletal System.............................................................................................................................31
Long Bone........................................................................................................................................32
Skull.................................................................................................................................................32
Vertebral Column.............................................................................................................................33
Thorax ( ...........................................................................................................................................33
Arm and Pectoral Girdle....................................................................................................................34
Pelvic Girdle.....................................................................................................................................34
Leg...................................................................................................................................................34
Arthology.....................................................................................................................................35
References:..................................................................................................................................38
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Laboratory Procedures
Science isa processbywhichwe gain an understandingof how thingswork.The studyof human
anatomyand physiologyisabiological science thatstudieshumanbodyfunctionandform.The human
bodyis wonderfullycomplex,andthe fieldof humanphysiologyisconstantlybeingstudiedandnew
processeselucidated.The abilitytoworkina cooperative manner,obtainandshare data,analyze results
and formconclusionswhilemaintainingasafe environmentiscritical wheninvestigatinghumanbody
functionandform.
I. KEY CONCEPTS
A. Describe the locationof safetyequipmentinyourlaboratoryclassroom.
B. Explainhowtouse a fire extinguisher.
C. Explainwhattodo inthe followingsituations:Breakingaglassbeakerduringlab,splashingchemical
inyour eye,hearingafire alarm.
D. List anddescribe the stepsof the scientificmethod.
E. Solve a problemusingthe scientificmethod.
F. Create a chart to record data.
G. Analyze andgrapha setof data.
H. Write a formal report.
II.Laboratory OrientationandSafety
INTRODUCTION:
Some of youhave extensive laboratoryexperience;however,forsome of youitmay have beenawhile
since youworkedinthistype of setting.Thisexercisewillhelpyoubecomecomfortable withsafety
guidelinesaswell asthe locationandproperuse of laboratoryequipment.
OBJECTIVES:The studentwill become familiarwiththe laboratorysettingandproperlaboratory
conduct;locate and learnhowto operate safetyequipment.
A. RoomOrientationandSafety - Tofamiliarize yourself withthe labroomanditsequipment,youwill
performa "safetyhunt”.Introduce yourself toyourlabmatesand worktogethertolocate the following
items:
Fire extinguisher;fume hood;exits(howmanyandwhere - be careful,some doorsmightleadtodead
ends);sinks;gasoutlets;emergencyphone;eye washstation;sharpscontainers;brokenglassware
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container,safetygoggles,firstaidkit,fire blanket,emergencycutoff forgas, chemical spill packet,
chemical burnstation,andnearestfire alarmpull
You are encouragedtoopendrawers,cabinetsanddoors.Some itemsmaybe locatedinanadjacentlab
or eveninthe hall.Make sure youare comfortable withthe properuse of the eyewashstation(washfor
a full 15 minutes);aswell asthe fire
extinguisher- the acronymPASSmay help(Pull pin,Aimatbase of fire,Squeeze trigger,Sweepside to
side).
B. SafetyContract:Before yournextlab,readcarefullythe LabSafetyGuidelinesprintedinthisbook.
You will be requiredtosignalab safetyaffidavitindicatingthatyouhave readandunderstandthe safety
guidelines.
Scientific Method
Scientificinvestigationsshare some commonelementsandprocedures,whichare referredtoasthe
scientificmethod.Notall scientistsfollowthese proceduresinastrictfashion,buteachof the elements
isusuallypresentinsome form.Science isacreative andcollaborativeprocessthatinvolvesasking
questions,developinganexploratoryhypothesis,andtestingthathypothesis.Professionalsinany
science field(appliedscience,healthscience,education,research,etc),andscience students,mustbe
able to use a type of thinkingcalledinductive reasoning.A personusingthiskindof thinkingobserves
manysmall facts andthenputs themtogethertoframe a large and broaderideaor thought.The ability
to collaborate,collectinformationwithoutbias,touse computerprogramstoanalyze data,and to
understandandcontrol variablesare fundamental critical thinkingskillsnecessaryforanystudentof
science.
Scientistscloselyscrutinize investigationsperformedbytheirpeers(peer-reviewed) andmustpresent
theirdata to variousorganizationsandinvariousways,providingevidence tosupportthe scientist’s
explanationof biological phenomena.All currentknowledge of how the humanbody worksisbasedon
evidence thathasbeenacknowledgedbyaconsensusof scientistsworldwide.Whenscientistsuse the
scientificmethoditensuresastandard,allowsotherstorepeatexperiments,andgivesusanswersto
questions.
OBJECTIVES:The student will conductaninvestigationusingthe scientificmethod.
SCIENTIC METHOD STEPS:
PROCEDURE: Your instructorwill pose aquestionregardingthe humanbodyinspiredbyreal events.
Work togetherasa classto answerthisquestionusingthe scientificmethod.Asagroup,decide what
methodyouwill use –what data will youneedtocollectandhow will yougoabout collectingthis
information?Howwill youcontrol variables?How muchdata isenoughto propose aconclusion?Next,
formulate ahypothesis –youcan do thisindividually.Afteranexperimentalmethodhasbeenproposed,
collectdatafrom and withyourclassmates.Alwaysremainaware of variables,andtryyour bestto
control them.
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You will be completingaformal labwrite-upwhenall of yourdatahas beencollectedandanalyzed
whichwill be includedinyourlabnotebook.
Investigation paper
Length of Forearm and Foot: Are they the same?
Our hypothesiswasthatourarm (fromelbow towrist) andour footwouldbe the same length.
Thislab wasdone so that we couldbecome familiarwithusingthe scientific
method.Todo thiswe firsthad to ask a question,andourquestionwas:isfoot
size andforearmlengththe same?We thenformulatedourhypothesis,andputit
to test,we thengatheredourconclusionand“reported”ourfindings.(Norman,
2011)
Thisproblemhasbeenan“old wivestale”fora longtime.Andithas always
appearedthatit wasat leastclose enoughtobe true,butconsideringthatitis
hard and awkwardtobring yourfootup to your arm andmeasure themnextto
each other,there isnoway to knowforsure if,in fact,your footand yourarm are
the same length.JustbygoogleingthisquestionIsaw that mostof the websites
say,“Yes! Of course,theyare the same go aheadand measure!”Itwouldappear
that thisstatementisalmosttakenasa fact of science.Evensome “science”
websiteswouldclaimourhypothesistobe a true statement.These were not
websitesthatcouldbe termedaswebsitesthatone woulduse asa reference for
a college essayorproject;sowe cannot assume thattheyare correct, evenif they
appearto be correctat firstglance.(Thomson,2012)
MaterialsandMethods.
For thisprojectwe onlyused:
1. yard stick
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2. feet
3. forearms
In orderto findoutif our hypothesiscouldbe provedordisprovedwe hadafew steps:
1. Measure the feetof the whole classbyplacingtheirfeetonthe floorand usingthe yardstick to
measure incentimetershowbigtheirfootwas.
2. We thenplacedthe yardstick onthe floorandmeasuredthe backof our forearmfromthe
elbowtothe wrist.
3. We thencompiledourdataby placingiton the white board.
Results
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The blue line isthe footlengthof eachstudentwhile the redline isthe
wristto elbowlength.The horizontal axisshow whichstudentforeachresult.And
the vertical axisshowsthe numberof centimetersmeasured.Thisgraphshows
that our resultswere notconsistent.Thereisachance that because of errorsour
hypothesiscouldstill be true.Butaccordingto the resultsthatwe have there is
no consistencyinthe waypeople’sfeetsize withtheirforearm.
Raw Data
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Withthisdata you are able tosee exactlyhow eachstudentcomparedhis orher shoe size totheir
forearm.
Discussion
I believethatourhypothesiswasneitherprovednordisproved.Becauseof
manypossible errorsandthe possibilitythatwe mayhave measuredwrongour
hypothesiscouldstill be correct.Ourerrorsincludednotusingthe same personto
measure all the otherpeople,bydoingthisitwouldhave greatlydecreasedour
chancesof errors.Anothererrorwasthat we believe thatthe sayingmeantforus
to measure fromthe inside of ourarms to see if ourfoot wasthe same size.
Althoughourexperimentcouldhave beenbetteritwasstill very
close to beingtrue.Manystudentshadthe same size arm as footwhile most
othersmeasurementswere different,butclose.
Investigation paper Revised
Lengthof Forearmand Foot: Are they the same?
Our hypothesiswasthatourarm (fromelbow towrist) andour footwouldbe the same length.
Thislab wasdone so that we couldbecome familiarwithusingthe scientific
method.Todo thiswe firsthad to ask a question,andourquestionwas:isfoot
size andforearmlengththe same?We thenformulatedourhypothesis,andputit
to test,we thengatheredourconclusionand“reported”ourfindings.(Reese,
2011)
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Thisproblemhasbeenan“old wivestale”fora long time.Andithas always
appearedthatit wasat leastclose enoughtobe true,butconsideringthatitis
hard and awkwardtobring yourfootup to your arm andmeasure themnextto
each other,there isnoway to knowforsure if,in fact,your footand yourarm are
the same length.(Size Wise 2013) Justby googleingthisquestionIsaw that most
of the websitessay,“Yes!Of course,theyare the same go aheadand measure!”It
wouldappearthatthisstatementisalmosttakenas a fact of science.Even some
“science”websiteswouldclaimourhypothesistobe a true statement.These
were notwebsitesthatcouldbe termedaswebsitesthatone woulduse asa
reference foracollege essayorproject;sowe cannot assume thattheyare
correct, evenif theyappeartobe correct at firstglance.(Norman,
2011)
MaterialsandMethods.
For thisprojectwe onlyused:
1. yard stick
2. feet
3. forearms
In orderto findoutif our hypothesiscouldbe provedordisprovedwe hadafew steps:
1. Measure the feetof the whole classbyplacingtheirfeetonthe floorand usingthe yardstick to
measure incentimetershowbigtheirfootwas.
2. We thenplacedthe yardstick onthe floorandmeasuredthe backof our forearmfromthe
elbowtothe wrist.
3. We then compiledourdataby placingiton the white board.
Results
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In thisgraph I useda scatterplotgraph so that we couldeasilysee the
comparisonbetweenthe lengthsof the armandthe lengthsof the foot.Inthis
graph the lengthof the footis representedbythe vertical axisandthe armis
representedbythe horizontalaxis.We cansee bylookingatthisgraph that
althoughmanyof the student’sfeetwere close tothe same lengththeywere not
exactlythe same length.
19
20
21
22
23
24
25
26
27
28
29
30
31
32
21 22 23 24 25 26 27 28 29 30 31
Heel
to
Toe
Length
Wrist to Elbow Length
Foot and Arm Length comparision
Foot and arm legnth
comparision
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Withthisdata you are able tosee exactlyhow eachstudentcomparedhisorher shoe size totheir
forearm.
Discussion
I believethatourhypothesiswasneitherprovednordisproved.Becauseof
manypossible errorsandthe possibilitythatwe mayhave measuredwrongour
hypothesiscouldstill be correct.Ourerrorsincludednotusingthe same personto
measure all the otherpeople,bydoingthisitwouldhave greatlydecreasedour
chancesof errors.Anothererrorwasthat we believe thatthe sayingmeantforus
Raw Data
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to measure fromthe inside of ourarms to see if ourfoot wasthe same size.
Althoughourexperimentcouldhave beenbetter(fixedthe errors,
thoughtit outlonger...etc.)itwasstill veryclose tobeingtrue.Manystudentshad
the same size arm as footwhile,butmoststudent’smeasurementswere
different.Althoughthe armandfoot lengthwere similartheywere notthe same
length.
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Light Microscope
Today inlabwe will be reviewingthe structure of the cell, identifyingmitosisphases,usinga
microscope,aswell asfindingthe total magnificationof the microscope.We will observe the mitosis
phasesusinga fishblastulaslide,andwe will be identifyinginterphase,prophase,metaphase,anaphase,
and telophase onthisslide. We will alsobe identifyingthe partsof the microscope.
Figure 1 (BiologyCorner,2012)
Light Microscope
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Figure 2 (Biology Corner, 2012)
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Mitosis
Interphase:The phase before mitosisstarts.Thisiswhenthe chromosomesare nottightlycoiled
as theywill be inthe nextphase.
Prophase:Thisiswhenthe spindlesformandbegintoseparate.Itisalsowhenthe
chromosomesbecome coiledtightly,andthe nuclear membrane startstodisintegrate.
There isalso a phase called“Prometaphase”thisphase iswhenthe chromosomesstartto
become alignedandthe spindlesgotoopposite endsof the cell.
Metaphase:thisiswhenthe chromosomesare alignedandthe microtubulesare attachedtothe
kinetochore.
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Anaphase:thisiswhenthe chromatidsare pulledapartandbroughtto differenthalvesof the cell.
Telophase:cellscleavage isformedandthe one cell startsto become two.The nucleusreforms,
chromosomesstartto uncoil.
The cell has nowbecome two.Itcan start the processall overagainand create more cellsafterithas
gone throughthe processof Interphase again.
(Gregory,M. J, 2013)
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Cytology
1. Keyconcepts:
a. Compare/contrastandexplainthe significance of the followingterms:karyokinesisandcytokinesis,
chromatinand chromosome,chromosomeandchromatid, centriole,centromere,aster
b. Describe howacell’sshape relatestoitsfunctionandgive examples
c. Explainthe significance of mitosisforhumancells
d. Define the terms“parfocal”and“fieldof view”
e.Illustrate whatoccursduringeach phase of mitosis
f.Calculate total magnificationof amicroscope.
2. Slides:Identifythe plasmamembrane,nucleus,cytoplasmonanytissue slide, Identifythe following
mitoticphasesina blastulaslide:interphase,prophase,metaphase,anaphase,telophase
3. Models/Equipment:
a. Identifythe followingpartsof a microscope anddescribe theirfunction:
Eyepiece (ocular),nosepiece,mechanical stage,base,arm, objectives, (scanning,low andhigh),fine
adjustment,coarse adjustment,condenser, diaphragm, bodytube
The Cell
Figure 3 (Armstrong, W.P, 2012)
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Nucleus-Enclosedbythe nuclearenvelope,the nucleuscontainsthe Chromatinaswell asthe nucleolus.
It containsmostof the genesinthe cell.Itisthe bestknownorganelleinthe animal cell.
Nucleolus-RNA issynthesizedinthe nucleolus.Proteinsare alsobroughtinandare thenmade into
ribosomes.
Plasmamembrane-Theoutermembranous“wall”thatcontainsthe cell.Itisalsoprovidesawayin and
out foronlycertainnutrients.
RoughEndoplasmicReticulum-Hasribosomesonthe outside.Itisusedtosecrete proteinsthatare
createdby the ribosomes.
SmoothEndoplasmicReticulum-Iscalledthe smoothERbecause of itlack of ribosomes.Itisusedto
store calciumions.It isalsohas enzymesthatdetoxifydrugs.Italsohasenzymesthatsynthesizevarious
formsof lipids.
Golgi apparatus-Actsasa “postal office”ittakesinproteinsthatare transportedwithvesicles,changes
the proteinsforthe functiontheyare neededforanditthenshipsitout throughanothervesicle.
Lysosome-Thisisasac that containsenzymesthatdigestmacromolecules.
Ribosome-Are made of RNA andproteins.Theyalsocarryout proteinsynthesis.
Vesicle-Transferproteinsthroughthe cytosol usuallybywayof the cytoskeleton.
Mitochondria-Isusedincellularrespirationtocreate ATPfromsugarsand fats.
Cytoskeleton-Retainscellshape.Andcontainsmicrofilaments,Intermediate filaments,and
microtubules,all of whichare made of protein.
(Reese,2011)
Histology and Integument
1. Keyconcepts:
a. Explainthe structure/functionrelationshipof all tissueslistedbelow.
b. Describe, ingeneral,howepithelial andtissuesare classified.
c. List the basictypesof connective tissues.
d. Outline the propertiesandfunctionsof the epidermisanddermis
e.Compare/contrasthairfollicle,hairroot,andhairshaft
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2. Slides:Identifythe followingtissues,andgive examplesof where theymightbe foundinthe human
body:
a. Epithelial tissues:
Simple squamous –example:lung
Simple cuboidal –examplekidney(tubules)
Simple columnar–example:ileum, duodenum, large intestine
Stratified squamous –example:skinoresophagus
Transitional - example:bladder(innerlining)
Pseudostratifidciliatedcolumnar–example:trachea(innerlining)
b. Connective tissues: Loose connective (areolar) –example:areolarspread(common)Adipose
connective –example:breast,hypodermis Denseregularconnective(white fibrous)–example:
ligamentsHyalinecartilage –example:trachea(rings) Elasticcartilage –example:earFibrocartilage–
example:intervertebral discs
*Note:althoughbone andbloodare connective tissues,theywill be addressedata latertime
c. Skinwithhairfollicle:stratifiedsquamousepithelium, hairroot,hairshaft,hairfollicle,dermal papilla,
adipose tissue
d. Muscle tissues:CardiacMuscle,SkeletalMuscle,SmoothMuscle
e.Nervoustissue:MotorNeuron,neuroglia
3. Models/Diagrams:
Identifythe followingstructuresfromadiagram or model:epidermis,stratumcorneum, stratum
lucidum,stratumgranulosum,stratumspinosum, stratumbasale,dermis,subcutaneouslayer
(hypodermis),adipose tissue,dermal papilla,Meissner’scorpuscle,Paciniancorpuscle,hairfollicle,hair
root,hair shaft,sebaceousgland,eccrine gland,arrectorpili muscle
Summary
Our goal for today’slabisto observe andmemorize different tissuesthroughamicroscope.We
will be lookingatepithelial tissues,connective tissues,muscles,nervous,andvarioushairfollicles.
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Epithelial Tissues
Epithelial Tissues are “membranous tissue covering internal organs and other internal surfaces of the
body” (Princeton 2013)
Simple Squamous Epithelial (Romstedt 2011)
Simple Cuboidal Epithelial (Romstedt 2011)
Simple Columnar Epithelial (Ramstedt 2011)
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Stratified Squamous Keratinized (Scarbrough 2013)
Stratified Squamous Non Keratinized (Frankhauser 2008)
Stratified Cuboidal (Scarbrough 2013)
Transitional (Scarbrough 2013)
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Pseudostratified Columnar (Ciliated) (Scarbrough 2013)
Connective Tissues
Connective tissues are “tissue of mesodermal origin consisting of e.g. collagen fibroblasts and fatty cel ls;
supports organs and fills spaces between them and forms tendons and ligaments ” (Princeton 2013)
Connective Tissue Proper
Loose Connective (Scarbrough 2013)
Adipose Connective (HFCC 2012)
Dense Regular Connective (Childs 2011)
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Dense Irregular Collagenous (Linggo 2012)
Dense Regular Elastic (Linggo 2012)
Cartilage
Hyaline Cartilage (Schaefer 2013)
Elastic Cartilage (HCCS 2005)
Fibrocartilage (Wynne 2012)
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Muscle Tissues
A muscle tissue is the tissue of the muscle.
Skeletal (Scarbrough 2013)
Cardiac (Scarbrough 2013)
Smooth (Scarbrough 2013)
Nervous
Nervous tissue is “tissue composed of neurons” (Princeton 2013)
Peripheral Nerve (Scarbrough 2013)
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Skin with Hair Follicle
A hairfollicle is“asmall tubularcavitycontainingthe rootof a hair; small musclesandsebaceous glands
are associated with them” (Princeton 2013)
Epidermis (tissue types 2012)
Stratified squamous epithelium
Stratum corneum
Stratum lucidum
Stratum granulosum
Stratum spinosum
Stratum basale
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Dermis (Shoummojit 2012)
Papillary layer
Dermal papillae
Reticular layer
Hair (Drugs Information Online 2012)
Follicle
Root
Shaft
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Arrector Pilli Muscle (Caceci 2012)
Integumentary
A. Epidermis- B.Stratumcorneum- C.Stratumlucidum- D.Stratumgranulosum- E.Stratumspinosum- F.
Stratumbasale- G. Dermis- H.Subcutaneouslayer(hypodermis)- I.Adipose tissue-J.Dermal papilla-K.
Meissner’scorpuscle-L.Paciniancorpuscle- M.Hairfollicle-N.Hairroot- O. Hair shaft- P.Sebaceous
gland- Q.Eccrine gland- R.Arrector pili muscle-
Integumentary (labeled) (Qui 2012)
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Integumentary (fill in the blank) (Qui 2012)
Anatomical Position and Planes
1. Key Concepts:
a. Describe the anatomical position.
b. Relate one body part to another using proper anatomical
directional terms. (ie. the sternum is medial to the clavicle.)
c. List the cavities of the body, and indicate the structures that reside in each cavity.
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d. Compare and contrast the anatomical planes and sections of the body.
2. Models/Diagrams/Slides
a. Anatomical Position
b. Anatomical Directional Terms
a. Dorsal,ventral,posterior,anterior,superior,inferior,medial,lateral, proximal, distal, cranial, caudal,
superficial, deep/profundus, external, internal
c. Planes
b. Median or median sagittal, sagittal, parasagittal/paramedian, coronal/frontal, horizontal
d. Sections
c. Longitudinal, transverse/cross
e. Anatomical Cavities
d. Dorsal, spinal, cranial, ventral
Anatomical Directional Terms (Micheau 2009)
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Planes (Abcfromfl.2010)
Cavities (MSU Denver 2012)
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Summary
For this lab we will be identifying and memorizing the anatomical positions and
terms.
1. KeyConcepts:
a. Describe howthe structure of a bone/bonystructure relates toitsstructure.Forexample,whywould
a bone have a raisedprojectionsuchasa tuberosityonit?
b. Compare andcontrast spongybone andcompact bone intermsof structure and function.
c. Explainwhybone tissue iscategorizedasconnective tissue.
d. Name the twoprocessesthatmake up the zygomaticarch.
e.Identifythe difference betweenthe axial andthe appendicularskeletons,andgive examplesof each
catagory
2. Slides:Identifycentral canal,osteon,lamella,canalliculi,lacunae andosteocytesonabone slide
3. Bones/Models/Diagrams:
a. BONESAND BONYFEATURES The namesof individual bonesare incapitals;the bone featuresare in
lowercase letters.Idenifyonabone or froma picture oneitherarticulatedordisarticulatedskeletons.
I. Skull
FRONTAL– frontal sinus,PARIETAL–sagittal suture,coronal suture,TEMPORAL– squamoussuture,
external auditorymeatus,styloidprocess,mastoidprocess,zygomaticprocess,mandibularcondyle
(fossa),OCCIPITAL–lambdoidsuture,foramenmagnum, occipitalcondyles,SPHENOID–greaterwings,
lesserwings,sellaturcica,sphenoidalsinuses, ETHMOID– crista galli,cribiformplates,superiorand
middle conchae (generally),perpendicularplate,ethmoidsinuses,MANDIBLE– body,rami,mandibular
condyle,coronoidprocess,alveolarprocess(border),MAXILLAE–alveolarprocess(border),palatine
process,maxillarysinuses,PALATINE,ZYGOMATIC– temporal process,zygomaticarch,LACRIMAL,
NASAL,VOMER,HYOID (onlyonarticulatedskeleton)
II.Vertebral Column
TYPICALVERTEBRA – body,lamina,pedicle,vertebral foramen,transverseprocess,spinousprocess,
superiorarticularprocess,inferiorarticularprocess,intervertebral foramen,CERVICALVERTEBRAE(7) –
C1, atlas; C2, axiswithdens(odontoidprocess);transverse foramen, THORACICVERTEBRAE(12) –
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articulatingfacetsforribs,LUMBAR VERTEBRAE (5) – large heavybodies,SACRUM(5 fusedvertebra) –
sacral foramina,sacral canal,COCCYX(usually4fusedvertebra)
III.Thorax
STERNUM – manubrium,body(gladiolus),xiphoidprocess,RIBS(12pairs) – identifytrue,false,and
floatingribs;costal cartilage
IV.Arm
HUMERUS – greatertubercle,lessertubercle,head,anatomical neck,deltoidtuberosity,trochlea,
capitulum,coronoidfossa,olecranonfossa,medial epicondyle,lateral epicondyle, RADIUS– head,radial
tuberosity,styloidprocess, ULNA – olecranonprocess,trochlear(semilunar) notch,coronoidprocess,
styloidprocess,CARPALS–generally,METACARPALS –generally,PHALANGES–generally
V.Pectoral Girdle
CLAVICLE– proximal (sternal) end,distal (acromial) end,SCAPULA –acromionprocess,coracoidprocess,
glenoidcavity,spine
VI.PelvicGirdle - Knowthe relationshipbetweenthe oscoxae (hipbones) andthe ilium, ischium,and
pubis
ILIUM – iliaccrest,anteriorsuperioriliacspine,posteriorsuperioriliacspine,anteriorinferioriliacspine,
posteriorinferioriliacspine,greaterischiadic(sciatic) notch,acetabulum, ISHIUM– ischial spine,lesser
ischiadic(sciatic) notch,ischialtuberosity,PUBIS–pubicsymphysis,obturatorforamen
VII.Leg
FEMUR – head,neck,greatertrochanter,lessertrochanter,medial epicondyle,lateral epicondyle,
medial condyle,lateral condyle,patellargroove (surface),TIBIA –medial condyle,lateral condyle,tibial
tuberosity,medial malleolus,FIBULA – head,lateral malleolus,PATELLA,TARSALS–onlyCALCANEUS
and TALUS specifically;the othersgenerally,METATARSALS –ingeneral,PHALANGES–ingeneral
b. PARTSOF A LONGBONE
Be able torecognize the following partsof along bone ondiagrams,bones,ormodels:
Proximal Epiphysis,DistalEpiphysis, Diaphysis,ArticularCartilage,Periosteum,CompactBone,
Cancellous(Spongy) Bone,MedullaryCavity,Endosteum,RedMarrow,Yellow Marrow, Epiphyseal Lines
(epiphyseal plates)
Skeletal System
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Long Bone (Girard 2011)
Skull (McPhail 2012)
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Vertebral Column (TutorVista 2010)
Thorax (Ahrendsen 2013)
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Arm and Pectoral Girdle (Creative Commons 2004)
Pelvic Girdle (e-sy.info. 2007)
Leg(Rauch 2012)
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Summary
We will be looking at various bone slides in order to identify the different bones.
We will also be identifying the different bones on the skeleton.
Arthology
1. Key Concepts:
a. Describea typicalsynovial joint, andrelateits structureto its function. Beableto give
examples.
b. Define amphiarthrosis, synarthrosis and diarthrosis
c. Explain the importanceoffontanels
2. Bones/Models/Diagrams:
a. Joint Classifications: Identify the following types ofjoints andgiveexamples ofeach:
I. Classification:
Synarthroses ( nonmoveable) i.e. sutures Amphiarthroses (slightlymovable) i.e. costal joints, pubicsymphysis Diarthrosis (freely movable): i.e.
all balland socketofhip,hinge ofknee
II. SynovialJointTypes
Ball and Socket Ellipsoid(condyloid) Plane (gliding) Hinge PivotSaddle
b. Synovial Joint -Identify and/or illustratethefollowing parts ofa freely moveableor
synovialjoint: JointCapsule Joint Cavity ArticularCartilage SynovialMembrane Spongy BoneofEpiphyses Bursa Meniscus
c. Knee –Identify the following parts on thekneejointmodelor diagram:
Medial and LateralCondyles ofFemurMedial and Lateral Condyles ofTibia Fibula Patella Patellar Ligament Tibial(Medial) CollateralLigament
Fibular (Lateral) CollateralLigament Anterior CruciateLigamentPosterior CruciateLigament
d. Shoulder -Identify thefollowing parts on the shoulder jointmodelor diagram:
GreaterTubercle ofHumerus Head ofHumerus Lesser TubercleofHumerus Distal(Acromion) endofClavicle Proximal(Sternal) end ofClavicle
Spine of Scapula Acromion process ofScapula Corocoidprocess ofScapula CoracoacromialLigament CorocohumeralLigament Coracoclavicular
LigamentGlenohumeral (Capsular) Ligaments,generally
e. Fetal Skull-Identify the following joints ona fetalskull model or diagram:
Frontal (anterior) fontanel Sphenoidal (anterolateral) fontanel Occipital(posterior) fontanel Mastoid (posterolateral) fontanel
P a g e | 36
Arthrology
Lori Gardner
March 5, 2013
“synovial joint, a freelymovable joint inwhich contiguous bonysurfacesare coveredbyarticular cartilage and connected bya
fibrous connective tissue capsule lined withsynovialmembrane. Kinds ofsynovial joints are ball andsocket joint, condyloid
joint, glidingjoint, hinge joint, pivot joint, saddle joint, anduniaxialjoint. Compare cartilaginous joint, fibrous joint.” (Mosby
2009)
“Amphiarthrosis a joint in whichthe surfacesare connectedbydisks of fibrocartilage, as betweenvertebrae.”(Miller 2003)
“Synarthrosis, n a joint formedbythin intervening layers ofcartilage, connective tissue, or direct contact of bone to bone. It
results ina rigidunion, andlittle movement of the bones occurs except during growth. Suture lines maybe obliterated in
adults, witha synarthrodial joint whenthe bonesjoined together become fused” (Mosby2009)
“Diarthrosis, a specializedform ofarticulationinwhich there is more or less free movement, the unionof the bonyelements
being surrounded byanarticular capsule enclosing a cavitylinedbysynovial membrane; called also synovial joint” (Saunders
2007)
Fontanels are the “soft spot” ona baby’s head. Withit the baby’s headis able to fit throughthe birthcanal and allows the brain
to grow bigger as the babyages.
Synarthroses, aphiarthrosis, and diarthrosis joint (University of Michigan, 200
Flexion
A movement decreasingthe angle between
articulatingbones.
(Some texts expressthis as "decreasingthe inner
angle of the joint".)
Extension
A movement increasing the angle between
articulatingbones.
Another wayto expressthis is "increasing the inner
angle of the joint".
Hyperextension
A movement to increase the angle between
articulatingbones to take a body-part or limb
beyond its normalrange.
Some of the types of joints that canpermit
extension (above).
Abduction
A movement awayfrom the mid-line of the body.
Adduction
A movement towards the mid-line of the body-
also applies to movements inwards andacrossthe
body.
Circumduction
Ivy Rose, 2013
P a g e | 37
Synovial joints (Pearson 2011)
Pearson Education, 2011
Driver, C 2011
(unpacking mystery, 2010)
P a g e | 38
Ligaments of scapula Fetal Skull
(Euxton Hall Hospital 2009)
Moeger 2010
References:
Norman C (2011) General Biology Lab Manual
Gregory, M. J. (2013,January). Mitosis and Meiosis. In TheBiology Web. RetrievedJanuary 31,2013, from
http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20101/bio%20101%20laboratory/mitosis/mitosis.htm
P a g e | 39
How to use a microscope. (2012(?)). In Biology Corner .com.Retrieved January30,2013, from
http://www.biologycorner.com/worksheets/microscope_use.html
Armstrong, W.P. (2012, January 22). ComparisonofPlant& Animal Cells. In Wayne's Word. Retrieved January 31, 2013,from
http://waynesword.palomar.edu/lmexer1a.htm
Reese, (2011) CampbellBiology, (9thed.) San Francisco,CA. Pearson Education,Inc.
References:
Princeton University . (2013).EpithelialTissues.In Word Net. Retrieved February 6, 2013,from
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Romstedt, K. (2011). SimpleEpithelial. In Biology 231 -Anatomy and Physiology I.Retrieved February 6,2013, from
http://capital2.capital.edu/faculty/kromsted/histology/simple.htm
Scarbrough, M. (2013). Slide 14StratifiedSquamous. InHistoWeb.Retrieved February 6,2013, from
http://www.kumc.edu/instruction/medicine/anatomy/histoweb
Fankhauser, D.(2008,October 16). Integumentary Histology. InINTEGUMENTARY SYSTEM. Retrieved February 6, 2013,from
http://biology.clc.uc.edu/fankhauser/Labs/Anatomy_&_Physiology/A&P201/Integumentary/Integument.htm
Henry Ford Community College . (2012). Adipose. In ScienceDivision at HFCC. Retrieved February 6, 2013,from
http://sciweb.hfcc.edu/Biology/AP/134/lab/lab%20guide%20images/Histology/adipose.ct.html
Childs, G.V. (2011, February). DenseRegular ConnectiveTissue . In DENSECONNECTIVETISSUE. Retrieved February 6, 2013,from
http://microanatomy.net/connective_tissue/dense_connective_tissue.htm
LINGGO, E. (2012). TISSUES, GLANDS,and MEMBRANES. In Principles ofHuman AnatomyandPhysiology . RetrievedFebruary6, 2013, from
http://hap1nuo1group3.blogspot.com/2012/01/chapter-4.html
Schaefer,W. (2013?). Tissues. InUniversity ofWisconsin. Retrieved February 6, 2013,from
http://washington.uwc.edu/about/wayne.schaefer/TissuesPage.htm
HCCS. (2005). Tissues. In Life Sciences . RetrievedFebruary6, 2013, fromhttp://swc2.hccs.edu/biol/labs/ap1/tissues/elasticCart.htm
Wynne, K. (2012(?)). ConnectiveTissues.In Introductory BiologyII -Human Biology . RetrievedFebruary 6, 2013, from
http://science.tjc.edu/Course/BIOLOGY/1409/1409connective.htm
Tissue Types . (2012 (?)). In Ocular Pathology . RetrievedFebruary 6, 2013, fromhttp://eyepathology.blogspot.com/
Shoummojit, R. (2012, July). Papillary Dermis. InHumanAnatomy -KnowYourBody.net. Retrieved February 6, 2013,from
http://www.calvin.edu/library/knightcite/index.php
Caceci,T. (2012 (?)). Integument System II: Hair.In Veterinary Histology. Retrieved February 6, 2013,from
http://www.vetmed.vt.edu/education/curriculum/vm8054/labs/lab15/lab15.htm
Definition of «Arrectorpili». (2012(?)). In Drugs InformationOnline. RetrievedFebruary 6, 2013, from
http://drugline.org/medic/term/arrector-pili/
References:
Size Wise.(2013). InScienceNet links. RetrievedFebruary 5,2013, from http://sciencenetlinks.com/afterschool-resources/size_wise/
Thomson, Judy. "LabReport One."Arapahoe CommunityCollege.Littleton. Sept. 2012.Lecture.
Qui, H. (2012). TheIntegumentary System. In Methamphetamine:ABiologicalApproach. RetrievedFebruary 7, 2013, from
http://methiscool.wordpress.com/the-integumentary-system/
P a g e | 40
Girard, . (2011). Long BoneDiagram. In Anatomy, Physiology,& Pathophysiology. Retrieved February 14,2013, from
http://phsgirard.org/Anatomy.html
Rauch, E. (2012). TheAmazingly Designed Human Body –Part 3 Read more:http://creationrevolution.com/2011/04/the-amazingly-designed-
human-body-%e2%80%93-part-3/#ixzz2Kqf8Bru5.In CreationRevolution . RetrievedFebruary14,2013, from
http://creationrevolution.com/2011/04/the-amazingly-designed-human-body-%E2%80%93-part-3/
e-sy.info. (2007). Anatomy And Physiology. In BEAUTY/ HOLISTIC THERAPIES.Retrieved February 14, 2013,from http://take-shape-
share.fenc.org.uk/providers/esyinfo/Vocational/Interactive%20and%20Web%20Links/Anatomy%20and%20Physiology/Anatomy%20and%20Ph
ysiology/18d.html
CreativeCommons . (2004).Visual Arm Bones Diagram. In DentalArticles . RetrievedFebruary 14, 2013,from
http://www.dentalarticles.com/visual/d/arm-bones-diagram.php
Ahrendsen, J.(2013). SternumAnatomy Diagram. InHealthApp. RetrievedFebruary14,2013, from
https://www.healthtap.com/#topics/sternum-anatomy-diagram
TutorVista. (2010). The Vertebral Column Or SpinalColumn. In TutorVista.com.Retrieved February 13, 2013,from
http://www.tutorvista.com/content/biology/biology-iv/locomotion-animals/vertebral-spinal-column.php
McPhail,B. J. (2012(?)). Skulls andSkeletons: Homology, Analogy, and Taxonomy. In Courses and Programs. Retrieved February 13, 2013, from
http://www.asms.net/facultymanaged/bmcph/courses/biochg/skullab.html
MSU Denver. (2012). Bio 2310 Human Anatomy andPhysiology I. Retrieved February 14, 2013, from
http://rowdy.msudenver.edu/~raoa/rao/bio2310/2310index.htm
Micheau,. (2009). E-ANATOMY: PLANES AND MOTIONS USED IN ANATOMY. In IMAIOS. Retrieved February 14, 2013, from
http://www.imaios.com/en/e-Anatomy/Limbs/Planes-and-motions-diagrams
Abcfromfl. (2010). Abcfromfl’s Anatomy: Anatomical Terms: Body Reference Planes. In Abcfromfl’s blog. RetrievedFebruary14,2013from
http://abcfromfl.wordpress.com/2010/05/27/abcfromfls-anatomy-anatomical-terms-body-reference-planes
Mosby's Medical Dictionary.8thed.2009. Print.
Miller-Keane Encyclopedia and DictionaryofMedicine,Nursing, and AlliedHealth, Seventh Edition.© 2003 by Saunders, an imprintof Elsevier,
Inc
Saunders ComprehensiveVeterinary Dictionary, 3 ed. © 2007Elsevier, Inc
Joint Movements. (2013, March5). In Ivyrose Holistic. RetrievedMarch 5,2013, from http://www.ivy-
rose.co.uk/HumanBody/Skeletal/Joints/Joint-Movements.php
University of MichiganHealthDepartment . (2002). Introduction toJoints.In Learning Modules -Medical Gross Anatomy. Retrieved March5,
2013, from http://www.med.umich.edu/lrc/coursepages/m1/anatomy2010/html/modules/joints_module/joints_02.html
Pearson Education Inc. (2011). SynovialJoints Diagram. In Articulations.Retrieved March 5, 2013,from
http://www.highlands.edu/academics/divisions/scipe/biology/faculty/harnden/2121/images/synovialtypes.jpg
Driver, C. (2011, November 16). Knee Pain.In MedicineNet.com. Retrieved March5, 2013, from
http://www.medicinenet.com/knee_pain/article.htm
Unpacking a mystery:. (2010). Inbegin todig. Retrieved March5, 2013, from http://www.begin2dig.com/2010/10/unpacking-mystery-when-
shoulder-pain.html
Euxton HallHospital. (2009). Shoulder Anatomy Ligaments . In Upper LimbCentre. RetrievedMarch 5,2013, from
http://www.upperlimbcentre.com/ligaments.htm
P a g e | 41
Moeger, L. (2010,November 21). Post for Nov.21.In For Landon. Retrieved March5, 2013,from http://forlandon.blogspot.com/

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Lab notebook

  • 1. Lab Notebook Bio 201 102 Terrence Harrison Lori Gardner
  • 2. P a g e | 1 Contents Laboratory Procedures....................................................................................................................2 INTRODUCTION:.............................................................................................................................2 Scientific Method............................................................................................................................3 SCIENTIC METHOD STEPS: ...............................................................................................................3 Investigation paper.........................................................................................................................4 Investigation paper Revised.............................................................................................................7 Light Microscope ..........................................................................................................................12 Light Microscope..............................................................................................................................12 Mitosis.........................................................................................................................................14 Cytology.......................................................................................................................................16 The Cell........................................................................................................................................16 Histology and Integument.............................................................................................................17 Epithelial Tissues...........................................................................................................................19 Connective Tissues........................................................................................................................21 Cartilage.......................................................................................................................................22 Muscle Tissues..............................................................................................................................23 Nervous .......................................................................................................................................23 Skin with Hair Follicle....................................................................................................................24 Integumentary..............................................................................................................................26 Anatomical Position and Planes.....................................................................................................27 Skeletal System.............................................................................................................................31 Long Bone........................................................................................................................................32 Skull.................................................................................................................................................32 Vertebral Column.............................................................................................................................33 Thorax ( ...........................................................................................................................................33 Arm and Pectoral Girdle....................................................................................................................34 Pelvic Girdle.....................................................................................................................................34 Leg...................................................................................................................................................34 Arthology.....................................................................................................................................35 References:..................................................................................................................................38
  • 3. P a g e | 2 Laboratory Procedures Science isa processbywhichwe gain an understandingof how thingswork.The studyof human anatomyand physiologyisabiological science thatstudieshumanbodyfunctionandform.The human bodyis wonderfullycomplex,andthe fieldof humanphysiologyisconstantlybeingstudiedandnew processeselucidated.The abilitytoworkina cooperative manner,obtainandshare data,analyze results and formconclusionswhilemaintainingasafe environmentiscritical wheninvestigatinghumanbody functionandform. I. KEY CONCEPTS A. Describe the locationof safetyequipmentinyourlaboratoryclassroom. B. Explainhowtouse a fire extinguisher. C. Explainwhattodo inthe followingsituations:Breakingaglassbeakerduringlab,splashingchemical inyour eye,hearingafire alarm. D. List anddescribe the stepsof the scientificmethod. E. Solve a problemusingthe scientificmethod. F. Create a chart to record data. G. Analyze andgrapha setof data. H. Write a formal report. II.Laboratory OrientationandSafety INTRODUCTION: Some of youhave extensive laboratoryexperience;however,forsome of youitmay have beenawhile since youworkedinthistype of setting.Thisexercisewillhelpyoubecomecomfortable withsafety guidelinesaswell asthe locationandproperuse of laboratoryequipment. OBJECTIVES:The studentwill become familiarwiththe laboratorysettingandproperlaboratory conduct;locate and learnhowto operate safetyequipment. A. RoomOrientationandSafety - Tofamiliarize yourself withthe labroomanditsequipment,youwill performa "safetyhunt”.Introduce yourself toyourlabmatesand worktogethertolocate the following items: Fire extinguisher;fume hood;exits(howmanyandwhere - be careful,some doorsmightleadtodead ends);sinks;gasoutlets;emergencyphone;eye washstation;sharpscontainers;brokenglassware
  • 4. P a g e | 3 container,safetygoggles,firstaidkit,fire blanket,emergencycutoff forgas, chemical spill packet, chemical burnstation,andnearestfire alarmpull You are encouragedtoopendrawers,cabinetsanddoors.Some itemsmaybe locatedinanadjacentlab or eveninthe hall.Make sure youare comfortable withthe properuse of the eyewashstation(washfor a full 15 minutes);aswell asthe fire extinguisher- the acronymPASSmay help(Pull pin,Aimatbase of fire,Squeeze trigger,Sweepside to side). B. SafetyContract:Before yournextlab,readcarefullythe LabSafetyGuidelinesprintedinthisbook. You will be requiredtosignalab safetyaffidavitindicatingthatyouhave readandunderstandthe safety guidelines. Scientific Method Scientificinvestigationsshare some commonelementsandprocedures,whichare referredtoasthe scientificmethod.Notall scientistsfollowthese proceduresinastrictfashion,buteachof the elements isusuallypresentinsome form.Science isacreative andcollaborativeprocessthatinvolvesasking questions,developinganexploratoryhypothesis,andtestingthathypothesis.Professionalsinany science field(appliedscience,healthscience,education,research,etc),andscience students,mustbe able to use a type of thinkingcalledinductive reasoning.A personusingthiskindof thinkingobserves manysmall facts andthenputs themtogethertoframe a large and broaderideaor thought.The ability to collaborate,collectinformationwithoutbias,touse computerprogramstoanalyze data,and to understandandcontrol variablesare fundamental critical thinkingskillsnecessaryforanystudentof science. Scientistscloselyscrutinize investigationsperformedbytheirpeers(peer-reviewed) andmustpresent theirdata to variousorganizationsandinvariousways,providingevidence tosupportthe scientist’s explanationof biological phenomena.All currentknowledge of how the humanbody worksisbasedon evidence thathasbeenacknowledgedbyaconsensusof scientistsworldwide.Whenscientistsuse the scientificmethoditensuresastandard,allowsotherstorepeatexperiments,andgivesusanswersto questions. OBJECTIVES:The student will conductaninvestigationusingthe scientificmethod. SCIENTIC METHOD STEPS: PROCEDURE: Your instructorwill pose aquestionregardingthe humanbodyinspiredbyreal events. Work togetherasa classto answerthisquestionusingthe scientificmethod.Asagroup,decide what methodyouwill use –what data will youneedtocollectandhow will yougoabout collectingthis information?Howwill youcontrol variables?How muchdata isenoughto propose aconclusion?Next, formulate ahypothesis –youcan do thisindividually.Afteranexperimentalmethodhasbeenproposed, collectdatafrom and withyourclassmates.Alwaysremainaware of variables,andtryyour bestto control them.
  • 5. P a g e | 4 You will be completingaformal labwrite-upwhenall of yourdatahas beencollectedandanalyzed whichwill be includedinyourlabnotebook. Investigation paper Length of Forearm and Foot: Are they the same? Our hypothesiswasthatourarm (fromelbow towrist) andour footwouldbe the same length. Thislab wasdone so that we couldbecome familiarwithusingthe scientific method.Todo thiswe firsthad to ask a question,andourquestionwas:isfoot size andforearmlengththe same?We thenformulatedourhypothesis,andputit to test,we thengatheredourconclusionand“reported”ourfindings.(Norman, 2011) Thisproblemhasbeenan“old wivestale”fora longtime.Andithas always appearedthatit wasat leastclose enoughtobe true,butconsideringthatitis hard and awkwardtobring yourfootup to your arm andmeasure themnextto each other,there isnoway to knowforsure if,in fact,your footand yourarm are the same length.JustbygoogleingthisquestionIsaw that mostof the websites say,“Yes! Of course,theyare the same go aheadand measure!”Itwouldappear that thisstatementisalmosttakenasa fact of science.Evensome “science” websiteswouldclaimourhypothesistobe a true statement.These were not websitesthatcouldbe termedaswebsitesthatone woulduse asa reference for a college essayorproject;sowe cannot assume thattheyare correct, evenif they appearto be correctat firstglance.(Thomson,2012) MaterialsandMethods. For thisprojectwe onlyused: 1. yard stick
  • 6. P a g e | 5 2. feet 3. forearms In orderto findoutif our hypothesiscouldbe provedordisprovedwe hadafew steps: 1. Measure the feetof the whole classbyplacingtheirfeetonthe floorand usingthe yardstick to measure incentimetershowbigtheirfootwas. 2. We thenplacedthe yardstick onthe floorandmeasuredthe backof our forearmfromthe elbowtothe wrist. 3. We thencompiledourdataby placingiton the white board. Results
  • 7. P a g e | 6 The blue line isthe footlengthof eachstudentwhile the redline isthe wristto elbowlength.The horizontal axisshow whichstudentforeachresult.And the vertical axisshowsthe numberof centimetersmeasured.Thisgraphshows that our resultswere notconsistent.Thereisachance that because of errorsour hypothesiscouldstill be true.Butaccordingto the resultsthatwe have there is no consistencyinthe waypeople’sfeetsize withtheirforearm. Raw Data
  • 8. P a g e | 7 Withthisdata you are able tosee exactlyhow eachstudentcomparedhis orher shoe size totheir forearm. Discussion I believethatourhypothesiswasneitherprovednordisproved.Becauseof manypossible errorsandthe possibilitythatwe mayhave measuredwrongour hypothesiscouldstill be correct.Ourerrorsincludednotusingthe same personto measure all the otherpeople,bydoingthisitwouldhave greatlydecreasedour chancesof errors.Anothererrorwasthat we believe thatthe sayingmeantforus to measure fromthe inside of ourarms to see if ourfoot wasthe same size. Althoughourexperimentcouldhave beenbetteritwasstill very close to beingtrue.Manystudentshadthe same size arm as footwhile most othersmeasurementswere different,butclose. Investigation paper Revised Lengthof Forearmand Foot: Are they the same? Our hypothesiswasthatourarm (fromelbow towrist) andour footwouldbe the same length. Thislab wasdone so that we couldbecome familiarwithusingthe scientific method.Todo thiswe firsthad to ask a question,andourquestionwas:isfoot size andforearmlengththe same?We thenformulatedourhypothesis,andputit to test,we thengatheredourconclusionand“reported”ourfindings.(Reese, 2011)
  • 9. P a g e | 8 Thisproblemhasbeenan“old wivestale”fora long time.Andithas always appearedthatit wasat leastclose enoughtobe true,butconsideringthatitis hard and awkwardtobring yourfootup to your arm andmeasure themnextto each other,there isnoway to knowforsure if,in fact,your footand yourarm are the same length.(Size Wise 2013) Justby googleingthisquestionIsaw that most of the websitessay,“Yes!Of course,theyare the same go aheadand measure!”It wouldappearthatthisstatementisalmosttakenas a fact of science.Even some “science”websiteswouldclaimourhypothesistobe a true statement.These were notwebsitesthatcouldbe termedaswebsitesthatone woulduse asa reference foracollege essayorproject;sowe cannot assume thattheyare correct, evenif theyappeartobe correct at firstglance.(Norman, 2011) MaterialsandMethods. For thisprojectwe onlyused: 1. yard stick 2. feet 3. forearms In orderto findoutif our hypothesiscouldbe provedordisprovedwe hadafew steps: 1. Measure the feetof the whole classbyplacingtheirfeetonthe floorand usingthe yardstick to measure incentimetershowbigtheirfootwas. 2. We thenplacedthe yardstick onthe floorandmeasuredthe backof our forearmfromthe elbowtothe wrist. 3. We then compiledourdataby placingiton the white board. Results
  • 10. P a g e | 9 In thisgraph I useda scatterplotgraph so that we couldeasilysee the comparisonbetweenthe lengthsof the armandthe lengthsof the foot.Inthis graph the lengthof the footis representedbythe vertical axisandthe armis representedbythe horizontalaxis.We cansee bylookingatthisgraph that althoughmanyof the student’sfeetwere close tothe same lengththeywere not exactlythe same length. 19 20 21 22 23 24 25 26 27 28 29 30 31 32 21 22 23 24 25 26 27 28 29 30 31 Heel to Toe Length Wrist to Elbow Length Foot and Arm Length comparision Foot and arm legnth comparision
  • 11. P a g e | 10 Withthisdata you are able tosee exactlyhow eachstudentcomparedhisorher shoe size totheir forearm. Discussion I believethatourhypothesiswasneitherprovednordisproved.Becauseof manypossible errorsandthe possibilitythatwe mayhave measuredwrongour hypothesiscouldstill be correct.Ourerrorsincludednotusingthe same personto measure all the otherpeople,bydoingthisitwouldhave greatlydecreasedour chancesof errors.Anothererrorwasthat we believe thatthe sayingmeantforus Raw Data
  • 12. P a g e | 11 to measure fromthe inside of ourarms to see if ourfoot wasthe same size. Althoughourexperimentcouldhave beenbetter(fixedthe errors, thoughtit outlonger...etc.)itwasstill veryclose tobeingtrue.Manystudentshad the same size arm as footwhile,butmoststudent’smeasurementswere different.Althoughthe armandfoot lengthwere similartheywere notthe same length.
  • 13. P a g e | 12 Light Microscope Today inlabwe will be reviewingthe structure of the cell, identifyingmitosisphases,usinga microscope,aswell asfindingthe total magnificationof the microscope.We will observe the mitosis phasesusinga fishblastulaslide,andwe will be identifyinginterphase,prophase,metaphase,anaphase, and telophase onthisslide. We will alsobe identifyingthe partsof the microscope. Figure 1 (BiologyCorner,2012) Light Microscope
  • 14. P a g e | 13 Figure 2 (Biology Corner, 2012)
  • 15. P a g e | 14 Mitosis Interphase:The phase before mitosisstarts.Thisiswhenthe chromosomesare nottightlycoiled as theywill be inthe nextphase. Prophase:Thisiswhenthe spindlesformandbegintoseparate.Itisalsowhenthe chromosomesbecome coiledtightly,andthe nuclear membrane startstodisintegrate. There isalso a phase called“Prometaphase”thisphase iswhenthe chromosomesstartto become alignedandthe spindlesgotoopposite endsof the cell. Metaphase:thisiswhenthe chromosomesare alignedandthe microtubulesare attachedtothe kinetochore.
  • 16. P a g e | 15 Anaphase:thisiswhenthe chromatidsare pulledapartandbroughtto differenthalvesof the cell. Telophase:cellscleavage isformedandthe one cell startsto become two.The nucleusreforms, chromosomesstartto uncoil. The cell has nowbecome two.Itcan start the processall overagainand create more cellsafterithas gone throughthe processof Interphase again. (Gregory,M. J, 2013)
  • 17. P a g e | 16 Cytology 1. Keyconcepts: a. Compare/contrastandexplainthe significance of the followingterms:karyokinesisandcytokinesis, chromatinand chromosome,chromosomeandchromatid, centriole,centromere,aster b. Describe howacell’sshape relatestoitsfunctionandgive examples c. Explainthe significance of mitosisforhumancells d. Define the terms“parfocal”and“fieldof view” e.Illustrate whatoccursduringeach phase of mitosis f.Calculate total magnificationof amicroscope. 2. Slides:Identifythe plasmamembrane,nucleus,cytoplasmonanytissue slide, Identifythe following mitoticphasesina blastulaslide:interphase,prophase,metaphase,anaphase,telophase 3. Models/Equipment: a. Identifythe followingpartsof a microscope anddescribe theirfunction: Eyepiece (ocular),nosepiece,mechanical stage,base,arm, objectives, (scanning,low andhigh),fine adjustment,coarse adjustment,condenser, diaphragm, bodytube The Cell Figure 3 (Armstrong, W.P, 2012)
  • 18. P a g e | 17 Nucleus-Enclosedbythe nuclearenvelope,the nucleuscontainsthe Chromatinaswell asthe nucleolus. It containsmostof the genesinthe cell.Itisthe bestknownorganelleinthe animal cell. Nucleolus-RNA issynthesizedinthe nucleolus.Proteinsare alsobroughtinandare thenmade into ribosomes. Plasmamembrane-Theoutermembranous“wall”thatcontainsthe cell.Itisalsoprovidesawayin and out foronlycertainnutrients. RoughEndoplasmicReticulum-Hasribosomesonthe outside.Itisusedtosecrete proteinsthatare createdby the ribosomes. SmoothEndoplasmicReticulum-Iscalledthe smoothERbecause of itlack of ribosomes.Itisusedto store calciumions.It isalsohas enzymesthatdetoxifydrugs.Italsohasenzymesthatsynthesizevarious formsof lipids. Golgi apparatus-Actsasa “postal office”ittakesinproteinsthatare transportedwithvesicles,changes the proteinsforthe functiontheyare neededforanditthenshipsitout throughanothervesicle. Lysosome-Thisisasac that containsenzymesthatdigestmacromolecules. Ribosome-Are made of RNA andproteins.Theyalsocarryout proteinsynthesis. Vesicle-Transferproteinsthroughthe cytosol usuallybywayof the cytoskeleton. Mitochondria-Isusedincellularrespirationtocreate ATPfromsugarsand fats. Cytoskeleton-Retainscellshape.Andcontainsmicrofilaments,Intermediate filaments,and microtubules,all of whichare made of protein. (Reese,2011) Histology and Integument 1. Keyconcepts: a. Explainthe structure/functionrelationshipof all tissueslistedbelow. b. Describe, ingeneral,howepithelial andtissuesare classified. c. List the basictypesof connective tissues. d. Outline the propertiesandfunctionsof the epidermisanddermis e.Compare/contrasthairfollicle,hairroot,andhairshaft
  • 19. P a g e | 18 2. Slides:Identifythe followingtissues,andgive examplesof where theymightbe foundinthe human body: a. Epithelial tissues: Simple squamous –example:lung Simple cuboidal –examplekidney(tubules) Simple columnar–example:ileum, duodenum, large intestine Stratified squamous –example:skinoresophagus Transitional - example:bladder(innerlining) Pseudostratifidciliatedcolumnar–example:trachea(innerlining) b. Connective tissues: Loose connective (areolar) –example:areolarspread(common)Adipose connective –example:breast,hypodermis Denseregularconnective(white fibrous)–example: ligamentsHyalinecartilage –example:trachea(rings) Elasticcartilage –example:earFibrocartilage– example:intervertebral discs *Note:althoughbone andbloodare connective tissues,theywill be addressedata latertime c. Skinwithhairfollicle:stratifiedsquamousepithelium, hairroot,hairshaft,hairfollicle,dermal papilla, adipose tissue d. Muscle tissues:CardiacMuscle,SkeletalMuscle,SmoothMuscle e.Nervoustissue:MotorNeuron,neuroglia 3. Models/Diagrams: Identifythe followingstructuresfromadiagram or model:epidermis,stratumcorneum, stratum lucidum,stratumgranulosum,stratumspinosum, stratumbasale,dermis,subcutaneouslayer (hypodermis),adipose tissue,dermal papilla,Meissner’scorpuscle,Paciniancorpuscle,hairfollicle,hair root,hair shaft,sebaceousgland,eccrine gland,arrectorpili muscle Summary Our goal for today’slabisto observe andmemorize different tissuesthroughamicroscope.We will be lookingatepithelial tissues,connective tissues,muscles,nervous,andvarioushairfollicles.
  • 20. P a g e | 19 Epithelial Tissues Epithelial Tissues are “membranous tissue covering internal organs and other internal surfaces of the body” (Princeton 2013) Simple Squamous Epithelial (Romstedt 2011) Simple Cuboidal Epithelial (Romstedt 2011) Simple Columnar Epithelial (Ramstedt 2011)
  • 21. P a g e | 20 Stratified Squamous Keratinized (Scarbrough 2013) Stratified Squamous Non Keratinized (Frankhauser 2008) Stratified Cuboidal (Scarbrough 2013) Transitional (Scarbrough 2013)
  • 22. P a g e | 21 Pseudostratified Columnar (Ciliated) (Scarbrough 2013) Connective Tissues Connective tissues are “tissue of mesodermal origin consisting of e.g. collagen fibroblasts and fatty cel ls; supports organs and fills spaces between them and forms tendons and ligaments ” (Princeton 2013) Connective Tissue Proper Loose Connective (Scarbrough 2013) Adipose Connective (HFCC 2012) Dense Regular Connective (Childs 2011)
  • 23. P a g e | 22 Dense Irregular Collagenous (Linggo 2012) Dense Regular Elastic (Linggo 2012) Cartilage Hyaline Cartilage (Schaefer 2013) Elastic Cartilage (HCCS 2005) Fibrocartilage (Wynne 2012)
  • 24. P a g e | 23 Muscle Tissues A muscle tissue is the tissue of the muscle. Skeletal (Scarbrough 2013) Cardiac (Scarbrough 2013) Smooth (Scarbrough 2013) Nervous Nervous tissue is “tissue composed of neurons” (Princeton 2013) Peripheral Nerve (Scarbrough 2013)
  • 25. P a g e | 24 Skin with Hair Follicle A hairfollicle is“asmall tubularcavitycontainingthe rootof a hair; small musclesandsebaceous glands are associated with them” (Princeton 2013) Epidermis (tissue types 2012) Stratified squamous epithelium Stratum corneum Stratum lucidum Stratum granulosum Stratum spinosum Stratum basale
  • 26. P a g e | 25 Dermis (Shoummojit 2012) Papillary layer Dermal papillae Reticular layer Hair (Drugs Information Online 2012) Follicle Root Shaft
  • 27. P a g e | 26 Arrector Pilli Muscle (Caceci 2012) Integumentary A. Epidermis- B.Stratumcorneum- C.Stratumlucidum- D.Stratumgranulosum- E.Stratumspinosum- F. Stratumbasale- G. Dermis- H.Subcutaneouslayer(hypodermis)- I.Adipose tissue-J.Dermal papilla-K. Meissner’scorpuscle-L.Paciniancorpuscle- M.Hairfollicle-N.Hairroot- O. Hair shaft- P.Sebaceous gland- Q.Eccrine gland- R.Arrector pili muscle- Integumentary (labeled) (Qui 2012)
  • 28. P a g e | 27 Integumentary (fill in the blank) (Qui 2012) Anatomical Position and Planes 1. Key Concepts: a. Describe the anatomical position. b. Relate one body part to another using proper anatomical directional terms. (ie. the sternum is medial to the clavicle.) c. List the cavities of the body, and indicate the structures that reside in each cavity.
  • 29. P a g e | 28 d. Compare and contrast the anatomical planes and sections of the body. 2. Models/Diagrams/Slides a. Anatomical Position b. Anatomical Directional Terms a. Dorsal,ventral,posterior,anterior,superior,inferior,medial,lateral, proximal, distal, cranial, caudal, superficial, deep/profundus, external, internal c. Planes b. Median or median sagittal, sagittal, parasagittal/paramedian, coronal/frontal, horizontal d. Sections c. Longitudinal, transverse/cross e. Anatomical Cavities d. Dorsal, spinal, cranial, ventral Anatomical Directional Terms (Micheau 2009)
  • 30. P a g e | 29 Planes (Abcfromfl.2010) Cavities (MSU Denver 2012)
  • 31. P a g e | 30 Summary For this lab we will be identifying and memorizing the anatomical positions and terms. 1. KeyConcepts: a. Describe howthe structure of a bone/bonystructure relates toitsstructure.Forexample,whywould a bone have a raisedprojectionsuchasa tuberosityonit? b. Compare andcontrast spongybone andcompact bone intermsof structure and function. c. Explainwhybone tissue iscategorizedasconnective tissue. d. Name the twoprocessesthatmake up the zygomaticarch. e.Identifythe difference betweenthe axial andthe appendicularskeletons,andgive examplesof each catagory 2. Slides:Identifycentral canal,osteon,lamella,canalliculi,lacunae andosteocytesonabone slide 3. Bones/Models/Diagrams: a. BONESAND BONYFEATURES The namesof individual bonesare incapitals;the bone featuresare in lowercase letters.Idenifyonabone or froma picture oneitherarticulatedordisarticulatedskeletons. I. Skull FRONTAL– frontal sinus,PARIETAL–sagittal suture,coronal suture,TEMPORAL– squamoussuture, external auditorymeatus,styloidprocess,mastoidprocess,zygomaticprocess,mandibularcondyle (fossa),OCCIPITAL–lambdoidsuture,foramenmagnum, occipitalcondyles,SPHENOID–greaterwings, lesserwings,sellaturcica,sphenoidalsinuses, ETHMOID– crista galli,cribiformplates,superiorand middle conchae (generally),perpendicularplate,ethmoidsinuses,MANDIBLE– body,rami,mandibular condyle,coronoidprocess,alveolarprocess(border),MAXILLAE–alveolarprocess(border),palatine process,maxillarysinuses,PALATINE,ZYGOMATIC– temporal process,zygomaticarch,LACRIMAL, NASAL,VOMER,HYOID (onlyonarticulatedskeleton) II.Vertebral Column TYPICALVERTEBRA – body,lamina,pedicle,vertebral foramen,transverseprocess,spinousprocess, superiorarticularprocess,inferiorarticularprocess,intervertebral foramen,CERVICALVERTEBRAE(7) – C1, atlas; C2, axiswithdens(odontoidprocess);transverse foramen, THORACICVERTEBRAE(12) –
  • 32. P a g e | 31 articulatingfacetsforribs,LUMBAR VERTEBRAE (5) – large heavybodies,SACRUM(5 fusedvertebra) – sacral foramina,sacral canal,COCCYX(usually4fusedvertebra) III.Thorax STERNUM – manubrium,body(gladiolus),xiphoidprocess,RIBS(12pairs) – identifytrue,false,and floatingribs;costal cartilage IV.Arm HUMERUS – greatertubercle,lessertubercle,head,anatomical neck,deltoidtuberosity,trochlea, capitulum,coronoidfossa,olecranonfossa,medial epicondyle,lateral epicondyle, RADIUS– head,radial tuberosity,styloidprocess, ULNA – olecranonprocess,trochlear(semilunar) notch,coronoidprocess, styloidprocess,CARPALS–generally,METACARPALS –generally,PHALANGES–generally V.Pectoral Girdle CLAVICLE– proximal (sternal) end,distal (acromial) end,SCAPULA –acromionprocess,coracoidprocess, glenoidcavity,spine VI.PelvicGirdle - Knowthe relationshipbetweenthe oscoxae (hipbones) andthe ilium, ischium,and pubis ILIUM – iliaccrest,anteriorsuperioriliacspine,posteriorsuperioriliacspine,anteriorinferioriliacspine, posteriorinferioriliacspine,greaterischiadic(sciatic) notch,acetabulum, ISHIUM– ischial spine,lesser ischiadic(sciatic) notch,ischialtuberosity,PUBIS–pubicsymphysis,obturatorforamen VII.Leg FEMUR – head,neck,greatertrochanter,lessertrochanter,medial epicondyle,lateral epicondyle, medial condyle,lateral condyle,patellargroove (surface),TIBIA –medial condyle,lateral condyle,tibial tuberosity,medial malleolus,FIBULA – head,lateral malleolus,PATELLA,TARSALS–onlyCALCANEUS and TALUS specifically;the othersgenerally,METATARSALS –ingeneral,PHALANGES–ingeneral b. PARTSOF A LONGBONE Be able torecognize the following partsof along bone ondiagrams,bones,ormodels: Proximal Epiphysis,DistalEpiphysis, Diaphysis,ArticularCartilage,Periosteum,CompactBone, Cancellous(Spongy) Bone,MedullaryCavity,Endosteum,RedMarrow,Yellow Marrow, Epiphyseal Lines (epiphyseal plates) Skeletal System
  • 33. P a g e | 32 Long Bone (Girard 2011) Skull (McPhail 2012)
  • 34. P a g e | 33 Vertebral Column (TutorVista 2010) Thorax (Ahrendsen 2013)
  • 35. P a g e | 34 Arm and Pectoral Girdle (Creative Commons 2004) Pelvic Girdle (e-sy.info. 2007) Leg(Rauch 2012)
  • 36. P a g e | 35 Summary We will be looking at various bone slides in order to identify the different bones. We will also be identifying the different bones on the skeleton. Arthology 1. Key Concepts: a. Describea typicalsynovial joint, andrelateits structureto its function. Beableto give examples. b. Define amphiarthrosis, synarthrosis and diarthrosis c. Explain the importanceoffontanels 2. Bones/Models/Diagrams: a. Joint Classifications: Identify the following types ofjoints andgiveexamples ofeach: I. Classification: Synarthroses ( nonmoveable) i.e. sutures Amphiarthroses (slightlymovable) i.e. costal joints, pubicsymphysis Diarthrosis (freely movable): i.e. all balland socketofhip,hinge ofknee II. SynovialJointTypes Ball and Socket Ellipsoid(condyloid) Plane (gliding) Hinge PivotSaddle b. Synovial Joint -Identify and/or illustratethefollowing parts ofa freely moveableor synovialjoint: JointCapsule Joint Cavity ArticularCartilage SynovialMembrane Spongy BoneofEpiphyses Bursa Meniscus c. Knee –Identify the following parts on thekneejointmodelor diagram: Medial and LateralCondyles ofFemurMedial and Lateral Condyles ofTibia Fibula Patella Patellar Ligament Tibial(Medial) CollateralLigament Fibular (Lateral) CollateralLigament Anterior CruciateLigamentPosterior CruciateLigament d. Shoulder -Identify thefollowing parts on the shoulder jointmodelor diagram: GreaterTubercle ofHumerus Head ofHumerus Lesser TubercleofHumerus Distal(Acromion) endofClavicle Proximal(Sternal) end ofClavicle Spine of Scapula Acromion process ofScapula Corocoidprocess ofScapula CoracoacromialLigament CorocohumeralLigament Coracoclavicular LigamentGlenohumeral (Capsular) Ligaments,generally e. Fetal Skull-Identify the following joints ona fetalskull model or diagram: Frontal (anterior) fontanel Sphenoidal (anterolateral) fontanel Occipital(posterior) fontanel Mastoid (posterolateral) fontanel
  • 37. P a g e | 36 Arthrology Lori Gardner March 5, 2013 “synovial joint, a freelymovable joint inwhich contiguous bonysurfacesare coveredbyarticular cartilage and connected bya fibrous connective tissue capsule lined withsynovialmembrane. Kinds ofsynovial joints are ball andsocket joint, condyloid joint, glidingjoint, hinge joint, pivot joint, saddle joint, anduniaxialjoint. Compare cartilaginous joint, fibrous joint.” (Mosby 2009) “Amphiarthrosis a joint in whichthe surfacesare connectedbydisks of fibrocartilage, as betweenvertebrae.”(Miller 2003) “Synarthrosis, n a joint formedbythin intervening layers ofcartilage, connective tissue, or direct contact of bone to bone. It results ina rigidunion, andlittle movement of the bones occurs except during growth. Suture lines maybe obliterated in adults, witha synarthrodial joint whenthe bonesjoined together become fused” (Mosby2009) “Diarthrosis, a specializedform ofarticulationinwhich there is more or less free movement, the unionof the bonyelements being surrounded byanarticular capsule enclosing a cavitylinedbysynovial membrane; called also synovial joint” (Saunders 2007) Fontanels are the “soft spot” ona baby’s head. Withit the baby’s headis able to fit throughthe birthcanal and allows the brain to grow bigger as the babyages. Synarthroses, aphiarthrosis, and diarthrosis joint (University of Michigan, 200 Flexion A movement decreasingthe angle between articulatingbones. (Some texts expressthis as "decreasingthe inner angle of the joint".) Extension A movement increasing the angle between articulatingbones. Another wayto expressthis is "increasing the inner angle of the joint". Hyperextension A movement to increase the angle between articulatingbones to take a body-part or limb beyond its normalrange. Some of the types of joints that canpermit extension (above). Abduction A movement awayfrom the mid-line of the body. Adduction A movement towards the mid-line of the body- also applies to movements inwards andacrossthe body. Circumduction Ivy Rose, 2013
  • 38. P a g e | 37 Synovial joints (Pearson 2011) Pearson Education, 2011 Driver, C 2011 (unpacking mystery, 2010)
  • 39. P a g e | 38 Ligaments of scapula Fetal Skull (Euxton Hall Hospital 2009) Moeger 2010 References: Norman C (2011) General Biology Lab Manual Gregory, M. J. (2013,January). Mitosis and Meiosis. In TheBiology Web. RetrievedJanuary 31,2013, from http://faculty.clintoncc.suny.edu/faculty/michael.gregory/files/bio%20101/bio%20101%20laboratory/mitosis/mitosis.htm
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