Random RNA interactions control protein expression in prokaryotesPaul Gardner
Presented at the NZSBMB/NZMS Conference in Christchurch 2016
CustomScience Award
A core assumption of gene expression analysis is that mRNA abundances broadly correlate with protein abundance, but these two can be imperfectly correlated. Some of the discrepancy can be accounted for by two important mRNA features: codon usage and mRNA secondary structure. We present a new global factor, called mRNA:ncRNA avoidance, and provide evidence that avoidance increases translational efficiency. We demonstrate a strong selection for the avoidance of stochastic mRNA:ncRNA interactions across prokaryotes, and that these have a greater impact on protein abundance than mRNA structure or codon usage. By generating synonymously variant green fluorescent protein (GFP) mRNAs with different potential for mRNA:ncRNA interactions, we demonstrate that GFP levels correlate well with interaction avoidance. Therefore, taking stochastic mRNA:ncRNA interactions into account enables precise modulation of protein abundance.
What Happens After You Are Pwnd: Understanding The Use Of Leaked Webmail Cred...Jeremiah Onaolapo
Slides of our work on understanding what happens to compromised webmail accounts. Presented at the ACM SIGCOMM Internet Measurement Conference in Santa Monica, CA. Blog post about it https://www.benthamsgaze.org/2016/11/14/understanding-the-use-of-leaked-webmail-credentials-in-the-wild/
Random RNA interactions control protein expression in prokaryotesPaul Gardner
Presented at the NZSBMB/NZMS Conference in Christchurch 2016
CustomScience Award
A core assumption of gene expression analysis is that mRNA abundances broadly correlate with protein abundance, but these two can be imperfectly correlated. Some of the discrepancy can be accounted for by two important mRNA features: codon usage and mRNA secondary structure. We present a new global factor, called mRNA:ncRNA avoidance, and provide evidence that avoidance increases translational efficiency. We demonstrate a strong selection for the avoidance of stochastic mRNA:ncRNA interactions across prokaryotes, and that these have a greater impact on protein abundance than mRNA structure or codon usage. By generating synonymously variant green fluorescent protein (GFP) mRNAs with different potential for mRNA:ncRNA interactions, we demonstrate that GFP levels correlate well with interaction avoidance. Therefore, taking stochastic mRNA:ncRNA interactions into account enables precise modulation of protein abundance.
What Happens After You Are Pwnd: Understanding The Use Of Leaked Webmail Cred...Jeremiah Onaolapo
Slides of our work on understanding what happens to compromised webmail accounts. Presented at the ACM SIGCOMM Internet Measurement Conference in Santa Monica, CA. Blog post about it https://www.benthamsgaze.org/2016/11/14/understanding-the-use-of-leaked-webmail-credentials-in-the-wild/
The Columbia University Data Science Institute's FinTech Lab launches The OpenSource Risk Engine and Risk DashBoard for academics, government regulators and financial market participants.
The Columbia University Data Science Institute's FinTech Lab launches The OpenSource Risk Engine and Risk DashBoard for academics, government regulators and financial market participants.