Technique
•Download as PPTX, PDF•
1 like•163 views
The document discusses an experiment testing the effects of four micronutrients (lithium, potassium, cobalt, and magnesium) on the percentage of Daphnia infected by the fungal parasite Metschnikowia. The hypothesis is that fewer nutrients would correlate to a lower percentage of infections. To test this, infections will be compared in nutrient-rich versus nutrient-poor lakes. Additionally, a controlled experiment using farmed Daphnia in an artificial habitat will aim to verify the results. Daphnia are farmed by creating an artificial freshwater mix and isolating adult Daphnia to produce clones for the experiment.
Report
Share
Report
Share
Recommended
coffee
Coffee plants evolved to produce caffeine as a defense against animals. A new study sequenced the genome of Coffea canephora and found that coffee plants produce caffeine through a series of enzymatic reactions that modify xanthosine. The enzymes involved evolved from pre-existing enzymes through gene duplication and mutation events over millions of years, allowing the plants to produce caffeine as a compound to control animal behavior and protect themselves.
Cultivo de daphnia sp y moina sp
La Unión Europea ha acordado un paquete de sanciones contra Rusia por su invasión de Ucrania. Las sanciones incluyen restricciones a los bancos rusos, la prohibición de la venta de aviones y equipos a Rusia, y sanciones contra funcionarios rusos. Los líderes de la UE esperan que las sanciones presionen a Rusia para que detenga su agresión contra Ucrania.
Daphnia
La pulga de agua (Daphnia) es un pequeño crustáceo transparente que vive en el plancton de agua dulce. Se reproduce de forma asexual mediante partenogénesis cuando las condiciones son favorables, pero de forma sexual cuando hay estrés, produciendo machos y formas resistentes. Su ciclo vital incluye tanto la reproducción asexual como la sexual, y se alimenta de bacterias y algas microscópicas.
Janet Seifert: What could a 'Whole Earth Stromatolite Catalogue' tell us?
This document discusses stromatolites and their importance for understanding microbial processes and environmental change over long time periods. Specifically, it mentions:
1) Stromatolite beds that are up to 600 million years old can provide insights into microbial life and Earth's early environments.
2) The formation of stromatolites depends on the bacterial community composition and environmental factors like the precipitation of calcium carbonate facilitated by microbial extracellular substances.
3) Living stromatolites exist today in locations like Cuatro Ciénegas, Mexico, which can serve as models to study microbial metabolic processes and their responses to environmental changes over time.
Cai shumei
1) The document discusses factors that affect the phosphorus absorbing efficiency of different duckweed genotypes, including light intensity, temperature, pH, and phosphorus concentration.
2) An experiment was conducted comparing three duckweed genotypes: Lemna aequinoctialis, Spirodela oligorrhiza, and Spirodela polyrrhiza. S. oligorrhiza had the highest phosphorus uptake rate despite a slower growth rate.
3) The results indicate that Spirodela genotypes are better suited than Lemna for removing phosphorus from wastewater, especially in conditions with higher phosphorus concentrations, making them a good candidate for wastewater treatment.
brandon presentation_v4
- The researchers were able to amplify and sequence archaeal and bacterial 16S rDNA from samples taken from a Bahamian beach and salt marsh, identifying organisms from the genera Haloferax and Halomonas.
- Haloferax species were isolated from 1.5M NaCl enrichment cultures of Bahamian beach sand, growing between 1.5-3.3M NaCl salinity. Halomonas species were also identified from the beach sand samples.
- The researchers aim to further analyze the microbial communities in their enrichment cultures over time to changing salinity conditions and identify additional novel species, helping elucidate how extreme halophiles can migrate between marine and hypersaline environments.
Studies on Protein Composition of Pigeon Pea [Cajanus Cajan (L.) Millspaugh] ...
The aim of this study was to determine the protein composition of pigeon pea Cajnus cajan (L)
millspaugh treated with sodium azide and gamma radiation. This was performed by exposing the seeds of
landraces pigeon pea to gamma rays at Centre for Radiotherapy and Oncology Department, ABUTH, Shika,
Zaria at doses of 0(control), 50, 100, 150 and 200Gy. These seeds were further treated with sodium azide
(NaN3) concentrations at 0.00, 0.01, 0.02, 0.03 and 0.04% SA. The analysis of protein content was the
determination of the amount of reduced Nitrogen present in food substance, i.e., its –NH2 and =NH2. The
proximate analyses were carried out as recommended by the Association of Official Analytical Chemists
(AOAC, 1990) using the kjeldahl method. The highest crude protein (CP) percentage value was obtained from
150Gy + 0.03%SA (25.1 %), followed by that due to 100Gy + 0.01%SA (24.7 %), 100Gy + 0.04%SA (24.4 %),
100Gy + 0.03%SA (23.7 %) and 150Gy + 0.00%SA (23.6 %). It is, therefore, concluded that the two mutagens
significantly affected the pigeon pea plant composition.
Algae for Conversion of Manure Nutrients to Animal Feed: Evaluation of Advanc...
Algae for Conversion of Manure Nutrients to Animal Feed: Evaluation of Advanc...National Institute of Food and Agriculture
This document summarizes a research project with 4 main tasks: [1] generate experimental data from algae bioreactors and calibrate optimization models, [2] maximize the nutritional value of algae for animal feed, [3] optimize pathogen inactivation methods, and [4] quantify and control toxic cyanobacteria. For task 1, the researchers collected seasonal data from outdoor bioreactors to develop productivity models. They identified dominant algae species and established laboratory protocols. For task 2, they isolated algae strains to optimize growth and nutritional composition. Tasks 3 and 4 will use these results to optimize pathogen inactivation and detect/control cyanotoxins.Recommended
coffee
Coffee plants evolved to produce caffeine as a defense against animals. A new study sequenced the genome of Coffea canephora and found that coffee plants produce caffeine through a series of enzymatic reactions that modify xanthosine. The enzymes involved evolved from pre-existing enzymes through gene duplication and mutation events over millions of years, allowing the plants to produce caffeine as a compound to control animal behavior and protect themselves.
Cultivo de daphnia sp y moina sp
La Unión Europea ha acordado un paquete de sanciones contra Rusia por su invasión de Ucrania. Las sanciones incluyen restricciones a los bancos rusos, la prohibición de la venta de aviones y equipos a Rusia, y sanciones contra funcionarios rusos. Los líderes de la UE esperan que las sanciones presionen a Rusia para que detenga su agresión contra Ucrania.
Daphnia
La pulga de agua (Daphnia) es un pequeño crustáceo transparente que vive en el plancton de agua dulce. Se reproduce de forma asexual mediante partenogénesis cuando las condiciones son favorables, pero de forma sexual cuando hay estrés, produciendo machos y formas resistentes. Su ciclo vital incluye tanto la reproducción asexual como la sexual, y se alimenta de bacterias y algas microscópicas.
Janet Seifert: What could a 'Whole Earth Stromatolite Catalogue' tell us?
This document discusses stromatolites and their importance for understanding microbial processes and environmental change over long time periods. Specifically, it mentions:
1) Stromatolite beds that are up to 600 million years old can provide insights into microbial life and Earth's early environments.
2) The formation of stromatolites depends on the bacterial community composition and environmental factors like the precipitation of calcium carbonate facilitated by microbial extracellular substances.
3) Living stromatolites exist today in locations like Cuatro Ciénegas, Mexico, which can serve as models to study microbial metabolic processes and their responses to environmental changes over time.
Cai shumei
1) The document discusses factors that affect the phosphorus absorbing efficiency of different duckweed genotypes, including light intensity, temperature, pH, and phosphorus concentration.
2) An experiment was conducted comparing three duckweed genotypes: Lemna aequinoctialis, Spirodela oligorrhiza, and Spirodela polyrrhiza. S. oligorrhiza had the highest phosphorus uptake rate despite a slower growth rate.
3) The results indicate that Spirodela genotypes are better suited than Lemna for removing phosphorus from wastewater, especially in conditions with higher phosphorus concentrations, making them a good candidate for wastewater treatment.
brandon presentation_v4
- The researchers were able to amplify and sequence archaeal and bacterial 16S rDNA from samples taken from a Bahamian beach and salt marsh, identifying organisms from the genera Haloferax and Halomonas.
- Haloferax species were isolated from 1.5M NaCl enrichment cultures of Bahamian beach sand, growing between 1.5-3.3M NaCl salinity. Halomonas species were also identified from the beach sand samples.
- The researchers aim to further analyze the microbial communities in their enrichment cultures over time to changing salinity conditions and identify additional novel species, helping elucidate how extreme halophiles can migrate between marine and hypersaline environments.
Studies on Protein Composition of Pigeon Pea [Cajanus Cajan (L.) Millspaugh] ...
The aim of this study was to determine the protein composition of pigeon pea Cajnus cajan (L)
millspaugh treated with sodium azide and gamma radiation. This was performed by exposing the seeds of
landraces pigeon pea to gamma rays at Centre for Radiotherapy and Oncology Department, ABUTH, Shika,
Zaria at doses of 0(control), 50, 100, 150 and 200Gy. These seeds were further treated with sodium azide
(NaN3) concentrations at 0.00, 0.01, 0.02, 0.03 and 0.04% SA. The analysis of protein content was the
determination of the amount of reduced Nitrogen present in food substance, i.e., its –NH2 and =NH2. The
proximate analyses were carried out as recommended by the Association of Official Analytical Chemists
(AOAC, 1990) using the kjeldahl method. The highest crude protein (CP) percentage value was obtained from
150Gy + 0.03%SA (25.1 %), followed by that due to 100Gy + 0.01%SA (24.7 %), 100Gy + 0.04%SA (24.4 %),
100Gy + 0.03%SA (23.7 %) and 150Gy + 0.00%SA (23.6 %). It is, therefore, concluded that the two mutagens
significantly affected the pigeon pea plant composition.
Algae for Conversion of Manure Nutrients to Animal Feed: Evaluation of Advanc...
Algae for Conversion of Manure Nutrients to Animal Feed: Evaluation of Advanc...National Institute of Food and Agriculture
This document summarizes a research project with 4 main tasks: [1] generate experimental data from algae bioreactors and calibrate optimization models, [2] maximize the nutritional value of algae for animal feed, [3] optimize pathogen inactivation methods, and [4] quantify and control toxic cyanobacteria. For task 1, the researchers collected seasonal data from outdoor bioreactors to develop productivity models. They identified dominant algae species and established laboratory protocols. For task 2, they isolated algae strains to optimize growth and nutritional composition. Tasks 3 and 4 will use these results to optimize pathogen inactivation and detect/control cyanotoxins.8
1) The study examined the ability of Lactobacillus plantarum MON03 (LP), Tunisian montmorillonite clay (TM), and their composite to remove the mycotoxin zearalenone (ZEA) in vitro and prevent its immunotoxic effects in mice.
2) Results showed that both TM and the LP+TM composite highly adsorbed ZEA (87.2% and 94.2% respectively) after 24 hours, while LP alone removed 78%. The amount of ZEA adsorbed was highest for the LP+TM composite.
3) In mice exposed to ZEA, treatment with LP, TM, or their composite prevented the immunotoxic effects of ZEA
Research_Report
This document summarizes research conducted on fungal laccase expression at the University of Houston Downtown. The research involved isolating RNA from nine laccase-producing fungi species. RNA was extracted using an RNeasy kit. Concentrations were determined using a spectrophotometer. A GeneRacer kit was used to amplify laccase cDNA ends through rapid amplification of cDNA ends PCR. The plan was to clone and characterize expressed laccases. Fungal samples were grown in malt extract agar and induced with veratryl alcohol before RNA extraction. The extracted RNA was used in dephosphorylation, decapping, oligo ligation, and reverse transcription steps of the GeneRacer protocol to ampl
MLC Cover Report V8c PDF
Preventine MLC is a larvicide made of minerals like gold, silver, copper, and zinc that kills mosquito larvae by destroying their cell walls and DNA. It also eliminates odor and kills bacteria in stagnant water within 3 hours, reducing the breeding of mosquitoes and house flies. Studies show it achieves 100% kill of mosquito larvae and eliminates E. coli and other pathogens in pig manure and wastewater, producing clear water suitable for agricultural use. Preventine MLC provides a natural alternative to insecticides for integrated mosquito control.
Final EHS 428 Poster
This study analyzed nutrient and bacterial concentrations in Bowne Park Pond over time. Algal blooms were previously observed, indicating potential eutrophication. Water samples were tested for nutrients and bacteria using various methods. Results showed high nutrient levels, likely due to surface runoff during heavy rainfall. Total coliform levels increased from 18 to 24 hours of incubation. Enterococcus levels remained relatively constant, suggesting it may be a more stable indicator of contamination than E. coli or total coliform. Future studies are needed to determine if the pond is prone to eutrophication, and if remedial actions are required to protect the marine habitat and public.
Lactobacillus acidophilus x Salmonella
1) A Lactobacillus acidophilus strain produces a molecule that kills Salmonella enterica serovar Typhimurium by damaging its bacterial membrane.
2) Exposure to this molecule leads to a decrease in intracellular ATP, increased membrane permeability, release of lipopolysaccharide from the membrane, and sensitization of the membrane to detergents.
3) This membrane damage mechanism is lethal to Salmonella and does not involve the PhoP-PhoQ or PmrA-PmrB regulatory systems that normally confer antimicrobial resistance.
Microbial food web manipulation in pond water for healthy shrimp
1) The document discusses manipulating the microbial food web in shrimp ponds to maintain healthy pond conditions. It focuses on bacteria and microalgae as the base of the food chain and important for nutrient cycles.
2) Two key aspects of the microbial food web that can be manipulated are the bacterial food web and microalgal food web. For bacteria, probiotics and postbiotics can help support beneficial bacteria and control pathogens. For microalgae, grazing can prevent population booms that disrupt the food chain.
3) Maintaining optimal environmental conditions like dissolved oxygen, pH, salinity, and water flow is important for the microbial food web to flourish. Regular harvesting of microbes also helps control the system
A pilot scale integrated seaweed (Ulva lactuca) & abalone (Haliotis midae) re...
A pilot scale integrated seaweed (Ulva lactuca) & abalone (Haliotis midae) re...Deborah Robertson-Andersson
This is a presentation I gave at the Applied Phycology congress in Galway Ireland in the IMTA session in 2008Corinne Breymeier Poster
The document examines the effects of acid on the chlorophyll production and biomass of common duckweed (Lemna minor L.). Two experiments were conducted exposing duckweed to pH levels of 4.1, 5.4, and 6.5 (control) over 10-12 days. The first experiment showed no significant differences in biomass between pH treatments. The second experiment found significantly lower biomass at pH 4.1 compared to pH 5.4 and 6.5. Neither experiment found significant differences in chlorophyll content between pH treatments. The results partially supported the hypothesis, showing acid inhibited biomass but not through impacts on chlorophyll. Longer exposure periods or lower pH levels may be needed to impact chlorophyll.
A study on the presence of fecal pollution indicator
This document summarizes a study on the presence of fecal indicator bacteria in Muttukadu back waters in Tamil Nadu, India. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for fecal coliforms using multiple tube fermentation and membrane filtration techniques. Analysis found higher numbers of fecal coliforms during the monsoon season, followed by post-monsoon, and lower numbers in pre-monsoon. Escherichia coli was identified as the dominant fecal indicator present throughout the year, indicating sewage contamination of the water body poses a health risk especially during monsoons.
A study on the presence of fecal pollution indicator
This document summarizes a study on the presence of fecal indicator bacteria in Muttukadu back waters in Tamil Nadu, India. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for fecal coliforms using multiple tube fermentation and membrane filtration techniques. Analysis found higher numbers of fecal coliforms during the monsoon season, followed by post-monsoon, and lower numbers in pre-monsoon. Escherichia coli was identified as the dominant fecal indicator present throughout the year, indicating sewage contamination of the water body poses a health risk especially during monsoons.
A study on the presence of fecal pollution indicator
This document summarizes a study on the presence of fecal indicator bacteria in Muttukadu back waters in Tamil Nadu, India. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for fecal coliforms using multiple tube fermentation and membrane filtration techniques. Analysis found higher numbers of fecal coliforms during the monsoon season, followed by post-monsoon, and lower numbers in pre-monsoon. Escherichia coli was identified as the dominant fecal indicator present throughout the year, indicating sewage contamination of the water body poses a health risk especially during monsoons.
International Journal of Hepatology & Gastroenterology
Loperamide (LOP) is an antidiarrheal agent that works by slowing gastrointestinal transit and reducing intestinal secretions. The aim of the study is to evaluate the effect of loperamide consumption for five days on the intestinal oxidative balance, as well as the putative protective effect of mallow leaves extract. Animals were divided into one normal control group and fi ve experimental groups. LOP, LOP + the different doses of the extract (100, 200, and 400 mg/ kg, b.w.), and LOP+ yohimbine (2 mg/ kg, b.w. p.i.), used as reference drug. Loperamide (3 mg/ kg, b.w. p.o) was administered twice a day, for 5 days. Treatment with mallow extract or yohimbine protected against the lipid peroxidation, antioxidant enzymes activity depletion, the fall in the thiol group and reduced glutathione level as well as jejunal free iron and H2O2 overload induced by loperamide intoxication. Thereby, Malva sylvestris aqueous extract (MSAE) attenuates the pathogenicity of loperamide.
Characterization of tannase- IJGEB
This document summarizes a study that characterized tannase production by Lactococcus lactis subsp lactis and its potential to enhance the nutritional value of composite sourdough. Key findings include:
1) L. lactis was able to tolerate tannin concentrations up to 0.25 mM and degrade 44.2% of tannins at 0.08 mM after 6 hours. Tannase production was inducible and the enzyme was detected intracellularly and extracellularly.
2) In a composite sourdough containing Vigna mungo flour and wheat flour, fermentation with L. lactis resulted in complete reduction of tannins (100%) and significant reduction of saponins
Job interview power Point
After several job applications, I finally got to get an interview for a tutor position. However, this time the interview was a bit different than all the others I had attended as a viewer. Most interviews were, as a rule, a presentation of the candidate skills and experience matching the job description. Mine had to be different, and more challenging.
The rules:
Fifteen-minute presentation of a lecture on one of 3 subjects, aiming at level 1 students; followed by 10 minutes presentation of my CV.
Here it is. Ohh, and by the way, I got the job.
MLC Cover Report V8b PDF
Preventine MLC is a larvicide produced by Preventine Ltd that uses minerals like gold, silver, copper, aluminum, and zinc to kill mosquito and fly larvae through various mechanisms of action. It also eliminates odor and kills bacteria in water. Testing showed it achieved 100% kill of mosquito larvae and elimination of house flies. The document provides details on its various applications and effects, including for treating wastewater and reducing associated diseases.
Microbial production of L-Asparaginase
The document summarizes a study on the microbial production of L-asparaginase by fungi. Fungal strains were isolated from soil samples and screened for their ability to produce L-asparaginase. Penicillium spp. showed the maximum production. The fermentation parameters like pH, temperature, and inoculum size were optimized. L-asparaginase was partially purified using ammonium sulfate precipitation. Thin layer chromatography confirmed enzymatic production. The purified enzyme demonstrated specificity for asparagine and maximum activity at pH 7 and 30°C. The study achieved microbial production and partial characterization of L-asparaginase using the fungal strain Penicillium spp.
Applications of Algal Technology for a Martian Colony *Fixed*
This document discusses potential applications of algal technology for sustainability on a Martian colony. It describes how algae could be used to produce oxygen through photosynthesis using carbon dioxide, water and nutrients. The algae could also be used in anaerobic digestion to produce methane and hydrogen from carbohydrates. The authors conducted experiments growing algae on anaerobic digester effluent, measuring oxygen production at different carbon dioxide concentrations and flow rates. They also measured nutrient removal by the algae and methane and hydrogen production. The results showed this method could supplement a Martian colony by producing oxygen and fuels, but challenges include adjusting the carbon dioxide concentration in the atmosphere.
IRJET- Ascertaining the Removal Efficiency of Lemna Minor in Froth Diminution...
This document summarizes a study that evaluated the removal efficiency of the duckweed species Lemna minor in reducing pollutants that cause foaming in wastewater. Lemna minor was inoculated into three wastewater samples and its removal efficiency of various pollutants like LAS, BOD, COD, phosphates, and nitrates was determined over 7 days. The removal efficiency ranged from 69-99% for different pollutants and samples. The dissolved oxygen of the water increased with treatment time. Lemna minor was effective at uptake of pollutants and can be used as a low-cost phytoremediation agent to reduce foaming in wastewater.
Copy of Kinetics Oyster Project.pdf
- Oysters were observed consuming algae and producing ammonia as a byproduct over a two hour period in a lab experiment. Standard curves were developed to measure algae and ammonia concentrations.
- The oysters filtered all the algae from the tank within an hour. Data showed the algae consumption followed zero order kinetics with a rate constant of -0.0022 g/L-min.
- Ammonia production increased over time and closely followed second order kinetics with a rate constant of -0.00035 L/mg-min. Forward models predicted how long full algae filtering and ammonia production would take.
Light House Retreats: Plant Medicine Retreat Europe
Our aim is to organise conscious gatherings and retreats for open and inquisitive minds and souls, with and without the assistance of sacred plants.
More Related Content
Similar to Technique
8
1) The study examined the ability of Lactobacillus plantarum MON03 (LP), Tunisian montmorillonite clay (TM), and their composite to remove the mycotoxin zearalenone (ZEA) in vitro and prevent its immunotoxic effects in mice.
2) Results showed that both TM and the LP+TM composite highly adsorbed ZEA (87.2% and 94.2% respectively) after 24 hours, while LP alone removed 78%. The amount of ZEA adsorbed was highest for the LP+TM composite.
3) In mice exposed to ZEA, treatment with LP, TM, or their composite prevented the immunotoxic effects of ZEA
Research_Report
This document summarizes research conducted on fungal laccase expression at the University of Houston Downtown. The research involved isolating RNA from nine laccase-producing fungi species. RNA was extracted using an RNeasy kit. Concentrations were determined using a spectrophotometer. A GeneRacer kit was used to amplify laccase cDNA ends through rapid amplification of cDNA ends PCR. The plan was to clone and characterize expressed laccases. Fungal samples were grown in malt extract agar and induced with veratryl alcohol before RNA extraction. The extracted RNA was used in dephosphorylation, decapping, oligo ligation, and reverse transcription steps of the GeneRacer protocol to ampl
MLC Cover Report V8c PDF
Preventine MLC is a larvicide made of minerals like gold, silver, copper, and zinc that kills mosquito larvae by destroying their cell walls and DNA. It also eliminates odor and kills bacteria in stagnant water within 3 hours, reducing the breeding of mosquitoes and house flies. Studies show it achieves 100% kill of mosquito larvae and eliminates E. coli and other pathogens in pig manure and wastewater, producing clear water suitable for agricultural use. Preventine MLC provides a natural alternative to insecticides for integrated mosquito control.
Final EHS 428 Poster
This study analyzed nutrient and bacterial concentrations in Bowne Park Pond over time. Algal blooms were previously observed, indicating potential eutrophication. Water samples were tested for nutrients and bacteria using various methods. Results showed high nutrient levels, likely due to surface runoff during heavy rainfall. Total coliform levels increased from 18 to 24 hours of incubation. Enterococcus levels remained relatively constant, suggesting it may be a more stable indicator of contamination than E. coli or total coliform. Future studies are needed to determine if the pond is prone to eutrophication, and if remedial actions are required to protect the marine habitat and public.
Lactobacillus acidophilus x Salmonella
1) A Lactobacillus acidophilus strain produces a molecule that kills Salmonella enterica serovar Typhimurium by damaging its bacterial membrane.
2) Exposure to this molecule leads to a decrease in intracellular ATP, increased membrane permeability, release of lipopolysaccharide from the membrane, and sensitization of the membrane to detergents.
3) This membrane damage mechanism is lethal to Salmonella and does not involve the PhoP-PhoQ or PmrA-PmrB regulatory systems that normally confer antimicrobial resistance.
Microbial food web manipulation in pond water for healthy shrimp
1) The document discusses manipulating the microbial food web in shrimp ponds to maintain healthy pond conditions. It focuses on bacteria and microalgae as the base of the food chain and important for nutrient cycles.
2) Two key aspects of the microbial food web that can be manipulated are the bacterial food web and microalgal food web. For bacteria, probiotics and postbiotics can help support beneficial bacteria and control pathogens. For microalgae, grazing can prevent population booms that disrupt the food chain.
3) Maintaining optimal environmental conditions like dissolved oxygen, pH, salinity, and water flow is important for the microbial food web to flourish. Regular harvesting of microbes also helps control the system
A pilot scale integrated seaweed (Ulva lactuca) & abalone (Haliotis midae) re...
A pilot scale integrated seaweed (Ulva lactuca) & abalone (Haliotis midae) re...Deborah Robertson-Andersson
This is a presentation I gave at the Applied Phycology congress in Galway Ireland in the IMTA session in 2008Corinne Breymeier Poster
The document examines the effects of acid on the chlorophyll production and biomass of common duckweed (Lemna minor L.). Two experiments were conducted exposing duckweed to pH levels of 4.1, 5.4, and 6.5 (control) over 10-12 days. The first experiment showed no significant differences in biomass between pH treatments. The second experiment found significantly lower biomass at pH 4.1 compared to pH 5.4 and 6.5. Neither experiment found significant differences in chlorophyll content between pH treatments. The results partially supported the hypothesis, showing acid inhibited biomass but not through impacts on chlorophyll. Longer exposure periods or lower pH levels may be needed to impact chlorophyll.
A study on the presence of fecal pollution indicator
This document summarizes a study on the presence of fecal indicator bacteria in Muttukadu back waters in Tamil Nadu, India. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for fecal coliforms using multiple tube fermentation and membrane filtration techniques. Analysis found higher numbers of fecal coliforms during the monsoon season, followed by post-monsoon, and lower numbers in pre-monsoon. Escherichia coli was identified as the dominant fecal indicator present throughout the year, indicating sewage contamination of the water body poses a health risk especially during monsoons.
A study on the presence of fecal pollution indicator
This document summarizes a study on the presence of fecal indicator bacteria in Muttukadu back waters in Tamil Nadu, India. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for fecal coliforms using multiple tube fermentation and membrane filtration techniques. Analysis found higher numbers of fecal coliforms during the monsoon season, followed by post-monsoon, and lower numbers in pre-monsoon. Escherichia coli was identified as the dominant fecal indicator present throughout the year, indicating sewage contamination of the water body poses a health risk especially during monsoons.
A study on the presence of fecal pollution indicator
This document summarizes a study on the presence of fecal indicator bacteria in Muttukadu back waters in Tamil Nadu, India. Water samples were collected during pre-monsoon, monsoon, and post-monsoon seasons and tested for fecal coliforms using multiple tube fermentation and membrane filtration techniques. Analysis found higher numbers of fecal coliforms during the monsoon season, followed by post-monsoon, and lower numbers in pre-monsoon. Escherichia coli was identified as the dominant fecal indicator present throughout the year, indicating sewage contamination of the water body poses a health risk especially during monsoons.
International Journal of Hepatology & Gastroenterology
Loperamide (LOP) is an antidiarrheal agent that works by slowing gastrointestinal transit and reducing intestinal secretions. The aim of the study is to evaluate the effect of loperamide consumption for five days on the intestinal oxidative balance, as well as the putative protective effect of mallow leaves extract. Animals were divided into one normal control group and fi ve experimental groups. LOP, LOP + the different doses of the extract (100, 200, and 400 mg/ kg, b.w.), and LOP+ yohimbine (2 mg/ kg, b.w. p.i.), used as reference drug. Loperamide (3 mg/ kg, b.w. p.o) was administered twice a day, for 5 days. Treatment with mallow extract or yohimbine protected against the lipid peroxidation, antioxidant enzymes activity depletion, the fall in the thiol group and reduced glutathione level as well as jejunal free iron and H2O2 overload induced by loperamide intoxication. Thereby, Malva sylvestris aqueous extract (MSAE) attenuates the pathogenicity of loperamide.
Characterization of tannase- IJGEB
This document summarizes a study that characterized tannase production by Lactococcus lactis subsp lactis and its potential to enhance the nutritional value of composite sourdough. Key findings include:
1) L. lactis was able to tolerate tannin concentrations up to 0.25 mM and degrade 44.2% of tannins at 0.08 mM after 6 hours. Tannase production was inducible and the enzyme was detected intracellularly and extracellularly.
2) In a composite sourdough containing Vigna mungo flour and wheat flour, fermentation with L. lactis resulted in complete reduction of tannins (100%) and significant reduction of saponins
Job interview power Point
After several job applications, I finally got to get an interview for a tutor position. However, this time the interview was a bit different than all the others I had attended as a viewer. Most interviews were, as a rule, a presentation of the candidate skills and experience matching the job description. Mine had to be different, and more challenging.
The rules:
Fifteen-minute presentation of a lecture on one of 3 subjects, aiming at level 1 students; followed by 10 minutes presentation of my CV.
Here it is. Ohh, and by the way, I got the job.
MLC Cover Report V8b PDF
Preventine MLC is a larvicide produced by Preventine Ltd that uses minerals like gold, silver, copper, aluminum, and zinc to kill mosquito and fly larvae through various mechanisms of action. It also eliminates odor and kills bacteria in water. Testing showed it achieved 100% kill of mosquito larvae and elimination of house flies. The document provides details on its various applications and effects, including for treating wastewater and reducing associated diseases.
Microbial production of L-Asparaginase
The document summarizes a study on the microbial production of L-asparaginase by fungi. Fungal strains were isolated from soil samples and screened for their ability to produce L-asparaginase. Penicillium spp. showed the maximum production. The fermentation parameters like pH, temperature, and inoculum size were optimized. L-asparaginase was partially purified using ammonium sulfate precipitation. Thin layer chromatography confirmed enzymatic production. The purified enzyme demonstrated specificity for asparagine and maximum activity at pH 7 and 30°C. The study achieved microbial production and partial characterization of L-asparaginase using the fungal strain Penicillium spp.
Applications of Algal Technology for a Martian Colony *Fixed*
This document discusses potential applications of algal technology for sustainability on a Martian colony. It describes how algae could be used to produce oxygen through photosynthesis using carbon dioxide, water and nutrients. The algae could also be used in anaerobic digestion to produce methane and hydrogen from carbohydrates. The authors conducted experiments growing algae on anaerobic digester effluent, measuring oxygen production at different carbon dioxide concentrations and flow rates. They also measured nutrient removal by the algae and methane and hydrogen production. The results showed this method could supplement a Martian colony by producing oxygen and fuels, but challenges include adjusting the carbon dioxide concentration in the atmosphere.
IRJET- Ascertaining the Removal Efficiency of Lemna Minor in Froth Diminution...
This document summarizes a study that evaluated the removal efficiency of the duckweed species Lemna minor in reducing pollutants that cause foaming in wastewater. Lemna minor was inoculated into three wastewater samples and its removal efficiency of various pollutants like LAS, BOD, COD, phosphates, and nitrates was determined over 7 days. The removal efficiency ranged from 69-99% for different pollutants and samples. The dissolved oxygen of the water increased with treatment time. Lemna minor was effective at uptake of pollutants and can be used as a low-cost phytoremediation agent to reduce foaming in wastewater.
Copy of Kinetics Oyster Project.pdf
- Oysters were observed consuming algae and producing ammonia as a byproduct over a two hour period in a lab experiment. Standard curves were developed to measure algae and ammonia concentrations.
- The oysters filtered all the algae from the tank within an hour. Data showed the algae consumption followed zero order kinetics with a rate constant of -0.0022 g/L-min.
- Ammonia production increased over time and closely followed second order kinetics with a rate constant of -0.00035 L/mg-min. Forward models predicted how long full algae filtering and ammonia production would take.
Similar to Technique (20)
Microbial food web manipulation in pond water for healthy shrimp
Microbial food web manipulation in pond water for healthy shrimp
A pilot scale integrated seaweed (Ulva lactuca) & abalone (Haliotis midae) re...
A pilot scale integrated seaweed (Ulva lactuca) & abalone (Haliotis midae) re...
A study on the presence of fecal pollution indicator
A study on the presence of fecal pollution indicator
A study on the presence of fecal pollution indicator
A study on the presence of fecal pollution indicator
A study on the presence of fecal pollution indicator
A study on the presence of fecal pollution indicator
International Journal of Hepatology & Gastroenterology
International Journal of Hepatology & Gastroenterology
Applications of Algal Technology for a Martian Colony *Fixed*
Applications of Algal Technology for a Martian Colony *Fixed*
IRJET- Ascertaining the Removal Efficiency of Lemna Minor in Froth Diminution...
IRJET- Ascertaining the Removal Efficiency of Lemna Minor in Froth Diminution...
Recently uploaded
Light House Retreats: Plant Medicine Retreat Europe
Our aim is to organise conscious gatherings and retreats for open and inquisitive minds and souls, with and without the assistance of sacred plants.
Phone Us ❤8107221448❤ #ℂall #gIRLS In Dehradun By Dehradun @ℂall @Girls Hotel...
Phone Us ❤8107221448❤ #ℂall #gIRLS In Dehradun By Dehradun @ℂall @Girls Hotel...chandankumarsmartiso
Phone Us ❤8107221448❤ #ℂall #gIRLS In Dehradun By Dehradun @ℂall @Girls Hotel With 100% Satisfaction
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
Journal Article Review on Rasamanikya
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
The Electrocardiogram - Physiologic Principles
These lecture slides, by Dr Sidra Arshad, offer a quick overview of the physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar lead (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
6. Describe the flow of current around the heart during the cardiac cycle
7. Discuss the placement and polarity of the leads of electrocardiograph
8. Describe the normal electrocardiograms recorded from the limb leads and explain the physiological basis of the different records that are obtained
9. Define mean electrical vector (axis) of the heart and give the normal range
10. Define the mean QRS vector
11. Describe the axes of leads (hexagonal reference system)
12. Comprehend the vectorial analysis of the normal ECG
13. Determine the mean electrical axis of the ventricular QRS and appreciate the mean axis deviation
14. Explain the concepts of current of injury, J point, and their significance
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. Chapter 3, Cardiology Explained, https://www.ncbi.nlm.nih.gov/books/NBK2214/
7. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Best Ayurvedic medicine for Gas and Indigestion
Here is the updated list of Top Best Ayurvedic medicine for Gas and Indigestion and those are Gas-O-Go Syp for Dyspepsia | Lavizyme Syrup for Acidity | Yumzyme Hepatoprotective Capsules etc
Top 10 Best Ayurvedic Kidney Stone Syrups in India
we have mentioned the best 10 ayurvedic Kidney Stone Syrups. You can check all these products and choose the best one for yourself.
Top-Vitamin-Supplement-Brands-in-India List
Swisschem Dermacare provides the Top 10 Vitamin Supplement Brands in India. To know more about us give us call at our official number
Histololgy of Female Reproductive System.pptx
Dive into an in-depth exploration of the histological structure of female reproductive system with this comprehensive lecture. Presented by Dr. Ayesha Irfan, Assistant Professor of Anatomy, this presentation covers the Gross anatomy and functional histology of the female reproductive organs. Ideal for students, educators, and anyone interested in medical science, this lecture provides clear explanations, detailed diagrams, and valuable insights into female reproductive system. Enhance your knowledge and understanding of this essential aspect of human biology.
ABDOMINAL TRAUMA in pediatrics part one.
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
The Best Ayurvedic Antacid Tablets in India
Treat the symptoms of indigestion, heartburn and stomach reflux with the 10 Best Ayurvedic Antacid Tablets in India.
Integrating Ayurveda into Parkinson’s Management: A Holistic Approach
Explore the benefits of combining Ayurveda with conventional Parkinson's treatments. Learn how a holistic approach can manage symptoms, enhance well-being, and balance body energies. Discover the steps to safely integrate Ayurvedic practices into your Parkinson’s care plan, including expert guidance on diet, herbal remedies, and lifestyle modifications.
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
Recently uploaded (20)
Light House Retreats: Plant Medicine Retreat Europe
Light House Retreats: Plant Medicine Retreat Europe
Phone Us ❤8107221448❤ #ℂall #gIRLS In Dehradun By Dehradun @ℂall @Girls Hotel...
Phone Us ❤8107221448❤ #ℂall #gIRLS In Dehradun By Dehradun @ℂall @Girls Hotel...
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
Does Over-Masturbation Contribute to Chronic Prostatitis.pptx
Top 10 Best Ayurvedic Kidney Stone Syrups in India
Top 10 Best Ayurvedic Kidney Stone Syrups in India
Integrating Ayurveda into Parkinson’s Management: A Holistic Approach
Integrating Ayurveda into Parkinson’s Management: A Holistic Approach
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...
Ear and its clinical correlations By Dr. Rabia Inam Gandapore.pptx
Ear and its clinical correlations By Dr. Rabia Inam Gandapore.pptx
Technique
- 1. Micronutrients in Epidemiology Hall Lab
- 2. Experimental focus Daphnia Dentifera MetschnikowiaBicuspidata Key facts: Fungal parasite Obligate killer Key facts: Zooplankton grazer Reproduce asexually
- 3. Micronutrient Ecology The goal of the experiment is to test the effects of four micronutrients (Lithium, Potassium, Cobalt, and Magnesium) on the percent of Daphnia infected. Our hypothesis is that fewer nutrients would correlate to a lower percentage of infections. Mo Li 40 30 20 10 0 0.5 1.5 2.5 Maximum percent infected 0 0.5 1.0 Co Mg 40 30 20 10 0 -0.8 -0.4 0 3.5 4.5 5.5 Log10(Ion, μg L-1) Figure 6. Relationships between maximum prevalence of infection during epidemics of a fungal parasite (Metschnikowia) in Daphnia dentifera populations and four ions in southern Indiana lakes. Li = lithium; Mo = molybdenum; Co = cobalt; Mg = magnesium. P and EPA Field Algal species 16 40 100% 25% 0% C A B 12 Lab 12 30 - EPA + EPA 8 Spore yield (x 104) 8 20 Shallow 4 4 10 Deep 0 0 0 -0.1 0.1 0.3 0.5 -0.1 0.7 0 0.3 0.2 0.4 0.6 Juvenile growth rate (day-1) Figure 4. Examples of the dependence of one epidemiological parameter (spore yield from dead, infected hosts) on resource quality, indexed by a juvenile growth rate assay. Hosts eating better quality, manipulated by (A) natural variation in the field, (B) species composition (100-0% of the easily digested Scenedesmusmixed with less digestible Oocystis, and (C) Scenedesmus with varying P-content alone (diamonds) and fatty acid addition with P (EPA, squares).
- 4. Techniques: Daphnia Farming We will compare infections in three nutrient rich lakes and three nutrient poor lakes. In addition, we will attempt to verify these results using a controlled experiment. To do this, we will need a massive amount of Daphnia, which we can farm in the lab. The two techniques required for creating a farm are: Creating an artificial freshwater habitat Harvesting Daphnia mothers
- 5. Artificial habitat The COMBO mix is made by combining MilliQ water (ultrapure lab-grade water) with various substances to imitate freshwater lake conditions. These substances include the ions commonly found in lake water and other molecules. In addition, Daphnia food, small algal particles called Scenedesmus, are added to ensure the mothers are well-fed.
- 6. HarvestingDaphnia mothers In order to reduce the effects of lurking variables in our experiment, we want to use identical, or nearly identical, Daphnia. To do this, we take eyedroppers and isolate the adult Daphnia. The adult Daphnia are placed into the artificial habitat created from COMBO. The babies (clones) produced in the next 24 hour period will be used as our experimental subjects.