Förstå dina kunder och besökare genom platstjänster. Med ökad insikt om dina kunder ökar värdet av kommunikationen. Idag använder vi tjänster som låter oss berätta helt öppet om vad vi gör, var vi är och vad vi tycker. Det här är en konversationen som du behöver förstå och delta i som företag. Genom att analysera den data som dina kunder genererar kan du få en inblick i vilka de är, vad de tycker om och vad de helst delar med sig av. Kunden är din bästa marknadsförare.
Chronic inflammation can cause immune cells like macrophages and lymphocytes to accumulate at the site of inflammation. When a cell is damaged or mutated, immune cells are stimulated to phagocytose the cell. However, in rare cases a cell can refuse to die and take on a new identity by recombining its original DNA with DNA from immune cells through crossovers and transfers of genetic material. This recombination of self and foreign DNA can potentially lead to the development of cancer if mutations are incorporated.
The document discusses 4 laws of carcinogenesis theory related to liver cancer. The 4 laws are: 1) Mutations group identity, 2) Inadequate energy, 3) Chronic inflammation, and 4) Normal DNA suppression. Mutations of cells and inadequate energy levels can lead to liver cancer formation, as can chronic inflammation and the suppression of normal DNA within liver cells.
DNA sequencing techniques have evolved over time. The dideoxy method developed by Sanger is useful for sequencing short DNA fragments of 500-750bp by terminating the chain with ddNTPs. Whole genome sequencing became possible using shotgun sequencing, which breaks the genome into random fragments that are sequenced and then reassembled. More recently, pyrosequencing was developed for sequencing by synthesis and allows accurate, parallel, and automated sequencing without the need for gel electrophoresis.
The document discusses Sanger sequencing, also known as dideoxy sequencing. It was developed by Fred Sanger in 1977 and allows scientists to determine the order of nitrogen bases (A, G, C, T) in a strand of DNA. The method involves using a primer, DNA polymerase, and dideoxynucleotides to produce terminated DNA fragments of different lengths, which are then separated by gel electrophoresis. This allows scientists to read the order of bases from the DNA sequence.
DNA sequencing is the process of determining the order of nucleotides in a DNA fragment. The first DNA sequencing method, called Sanger sequencing, was developed in 1975 and involves copying DNA fragments and labeling the fragments to determine the sequence. DNA sequencing is useful for scientists as it allows study of what DNA codes for through comparative and functional genomics. While it improves healthcare and agriculture, a disadvantage is the risk of providing an incorrect DNA sequence.
The document summarizes a presentation on DNA sequencing techniques. It describes DNA and its functions of storing genetic information, self-duplication, and expressing genetic messages. It then explains the historical Maxam-Gilbert and Sanger DNA sequencing methods and how modern sequencing uses variations of the Sanger technique. The document provides details on the chemical degradation approach of Maxam-Gilbert and the chain termination approach of Sanger sequencing before polymerase chain reaction and gel electrophoresis. It compares the two methods and discusses applications and future directions of DNA sequencing.
Förstå dina kunder och besökare genom platstjänster. Med ökad insikt om dina kunder ökar värdet av kommunikationen. Idag använder vi tjänster som låter oss berätta helt öppet om vad vi gör, var vi är och vad vi tycker. Det här är en konversationen som du behöver förstå och delta i som företag. Genom att analysera den data som dina kunder genererar kan du få en inblick i vilka de är, vad de tycker om och vad de helst delar med sig av. Kunden är din bästa marknadsförare.
Chronic inflammation can cause immune cells like macrophages and lymphocytes to accumulate at the site of inflammation. When a cell is damaged or mutated, immune cells are stimulated to phagocytose the cell. However, in rare cases a cell can refuse to die and take on a new identity by recombining its original DNA with DNA from immune cells through crossovers and transfers of genetic material. This recombination of self and foreign DNA can potentially lead to the development of cancer if mutations are incorporated.
The document discusses 4 laws of carcinogenesis theory related to liver cancer. The 4 laws are: 1) Mutations group identity, 2) Inadequate energy, 3) Chronic inflammation, and 4) Normal DNA suppression. Mutations of cells and inadequate energy levels can lead to liver cancer formation, as can chronic inflammation and the suppression of normal DNA within liver cells.
DNA sequencing techniques have evolved over time. The dideoxy method developed by Sanger is useful for sequencing short DNA fragments of 500-750bp by terminating the chain with ddNTPs. Whole genome sequencing became possible using shotgun sequencing, which breaks the genome into random fragments that are sequenced and then reassembled. More recently, pyrosequencing was developed for sequencing by synthesis and allows accurate, parallel, and automated sequencing without the need for gel electrophoresis.
The document discusses Sanger sequencing, also known as dideoxy sequencing. It was developed by Fred Sanger in 1977 and allows scientists to determine the order of nitrogen bases (A, G, C, T) in a strand of DNA. The method involves using a primer, DNA polymerase, and dideoxynucleotides to produce terminated DNA fragments of different lengths, which are then separated by gel electrophoresis. This allows scientists to read the order of bases from the DNA sequence.
DNA sequencing is the process of determining the order of nucleotides in a DNA fragment. The first DNA sequencing method, called Sanger sequencing, was developed in 1975 and involves copying DNA fragments and labeling the fragments to determine the sequence. DNA sequencing is useful for scientists as it allows study of what DNA codes for through comparative and functional genomics. While it improves healthcare and agriculture, a disadvantage is the risk of providing an incorrect DNA sequence.
The document summarizes a presentation on DNA sequencing techniques. It describes DNA and its functions of storing genetic information, self-duplication, and expressing genetic messages. It then explains the historical Maxam-Gilbert and Sanger DNA sequencing methods and how modern sequencing uses variations of the Sanger technique. The document provides details on the chemical degradation approach of Maxam-Gilbert and the chain termination approach of Sanger sequencing before polymerase chain reaction and gel electrophoresis. It compares the two methods and discusses applications and future directions of DNA sequencing.