This document describes a study that produced a powder inoculant using Lactobacillus pentosus (KUB ST 10-1) for ensiling. L. pentosus was grown in MRS broth for 12 hours and reached maximum growth at pH 5.5-7.0. The powder inoculant produced using L. pentosus showed significantly higher growth than the control without inoculant. The powder inoculant can be used to improve the fermentation and preservation of silage.