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Researchers wanted to study if 2 proteins Q and R come close enough to interact with each other
in the presence of high levels of cAMP. In a FRET experiment, protein Q was fused to CF and
protein R was fused to YFP. Both these gene fusions were transfected into COS cells. So, when
cAMP levels rise and the cells were illuminated using 440 m light, the predominant emission
was at 490m. How would you interpret this result?

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  • 1. Researchers wanted to study if 2 proteins Q and R come close enough to interact with each other in the presence of high levels of cAMP. In a FRET experiment, protein Q was fused to CF and protein R was fused to YFP. Both these gene fusions were transfected into COS cells. So, when cAMP levels rise and the cells were illuminated using 440 m light, the predominant emission was at 490m. How would you interpret this result?