The document discusses renal function tests, their purposes in diagnosing and monitoring kidney health, and describes key parameters such as glomerular filtration rate (GFR) and creatinine clearance. It emphasizes the significance of various substances, including urea, creatinine, and cystatin C, as indicators of renal function, along with the impact of renal diseases on these markers. Additionally, it reviews urine characteristics and abnormalities that can indicate kidney dysfunction, including proteinuria and specific gravity variations.

1. Dr.S.Sethupathy, M.D,Ph.D.,
Professor of Biochemistry,
Rajah Muthiah Medical College,
Annamalai university

2. Renal function tests are done for :
 Diagnosis of renal disease
 Assessment of its prognosis
 Monitoring renal function and
damage.

3.  Excretory function
 Excretion of endogenous waste
products such as :
urea, uric acid, creatinine and
exogenous drugs, it metabolites,
toxins etc.

4. Maintenance of water
balance
Maintenance of electrolyte
balance
 Maintenance of acid base
balance

5.  Kidney Synthesizes erythropoietin
which stimulates erythropoiesis.
 Activation of 25-OH cholecalciferol
to 1,25 dihydroxy cholecalciferol
(Calcitriol) by 1-alpha hydroxylase
enzyme in the kidney which
promotes calcium absorption in the
intestine.

6.  Nephron is the functional unit of kidney which
has two components :
 1.Glomerulus
 2. Tubules
 Glomerulus is the filtration unit and tubules
are reabsorbing unit.

7.  Through Bowman’s capsule, an ultra-filtrate
which is devoid of cells and protein formed
 Albumin does not pass through the membrane.
So in case of glomerular damage, albumin
appears in urine.
 Glomerular filtration depends on renal blood
supply and functional state of nephrons.

8.  Glomerular filtration rate (GFR) is :
1. Decreased in hemorrhagic shock, anaphylactic
shock due to decreased perfusion
2. In glomerulonephritis due to dysfunction of
nephrons.
Decrease in GFR leads to retention and
accumulation of waste products such as urea,
creatinine and uric acid in the plasma.

9.  Plasma creatinine: Normal level is 0.6-1.2
mg/dl.
 It is a better indicator of GFR as it is not
influenced by diet or protein catabolism as in
case of urea.
 It is inversely related to GFR.
 Plasma urea : Normal plasma urea level is 15-
40 mg/dl.
 Plasma urea nitrogen is 7-18 mg/dl.

10.  PAH is filtered at the glomerulus as well as
secreted by the tubules
 Completely removed by the kidney by
single passage of blood through the kidney
 Renal perfusion flow(RBF)= Upah/ppah x V
V= volume of urine (ml/min)
normal – 600 ml min

12.  It is also an index of GFR.
 Pre renal failure - dehydration.
urea is increased more than
creatinine because it is reabsorbed
by the tubular cells.
 In renal failure, both will increase.
 Post renal uremia-both will increase

13.  Clearance is defined as the
volume of blood or plasma in ml
that is completely cleared of a
substance (in case of creatinine
clearance creatinine is cleared)
per unit time and is expressed as
ml per min.

14.  Creatinine clearance = UV/P
 U=concentration of creatinine in urine (mg/dl)
 P=concentration of creatinine in plasma
(mg/dl)
 V- volume of urine passed per minute
*ml/min).
 24 hours urine is collected and the total volume
is divided by 1440 .

15.  Inulin clearance : It is the reference method for
determination of GFR. It is not routinely done
because it is given exogenously and the
maintenance of its blood level requires
monitoring.
 Urea clearance
 It is lower than inulin clearance because urea is
reabsorbed in the tubules.

16.  Marker should be freely filtered
 It should be neither secreted nor reabsorbed
and it’s blood level should be constant.
 Creatinine being an endogenous substance its
level is maintained, freely filtered but a small
amount is secreted by the tubules (10% of
creatinine excreted is tubular component).

17.  Normal level is 0.5-1.0 mg/L.
 Cystatin C is produced at a constant rate and is
freely filtered by glomerulus.
 It is completely reabsorbed by the tubules and
degraded in the tubules.
 The blood level is not dependent upon age, sex,
muscle mass or inflammatory processes.
 It is a better indicator of GFR than creatinine
 Extremely sensitive to minor changes in GFR
during the course of chronic kidney disease.

18.  Normal value: male: 75-125 ml/min
 Female: 65-115 ml/min
 Decreased creatinine clearance is a very
sensitive indicator of reduced glomerular
filtration rate (GFR).
 In old age, the clearance is decreased.
 It is helpful in the early detection of functional
impairment of kidney and also for monitoring
the patients with renal insufficiency.

19.  GFR mL/minute = [(140 – age in
years) × (wt, kg)]/ 72 × P (mg/L) ×
0.85 (if subject is female).
 If converting to SI units (GFR in
micromoles per liter) is desired,
replace 72 in the denominator with
0.84.

20.  GFR (mL/minute/(1.73 m2)) = 186
× (serum creatinine [mg/dL]–1.154)
× (age in years)-0.203 × (0.742 if
female) × (1.210 if
African American).
 If converting to SI units (GFR in
micromoles per liter) is desired,
replace 186 with 32,788.

21.  Estimates GFR adjusted for body surface
area.
 Designed for use with laboratory
creatinine test.
 Is more accurate than creatinine clearance
measured from 24-hour urine collections
or estimated by the Cockcroft-Gault
formula.

23.  The glomerular membrane is impermeable to
albumin.
 Low molecular proteins are filtered reabsorbed
and catabolized by tubular cells.
 Albumin excretion is less than 30 mg/24 hours.
 Urine test for albumin is negative in normal
subjects.

24. It may be due to
 1.Increase in filtered load due to glomerular
damage and vascular permeability. It is called
glomerular proteinuria.
 2.Increased concentration of low molecular weight
proteins in circulation resulting in Overflow
proteinuria.
 3.Decrease in the tubular reabsorption of proteins
resulting in tubular proteinuria.

25.  Early morning urine specimen or 24hrs
urine specimen tested for albumin.
 Benign proteinuria- 300mg/day
 Pathological proteinuria- 300-1000
mg/day
 Glomerular proteinuria - > 1 gm/day.
 In nephrotic syndrome massive
proteinuria is seen >3 gm/day.
 Diabetic nephropathy,
 Chronic glomerulonephritis,
 Hypertension.

26.  If albumin is detected in a urine sample collected
at random, over 4 hours, or overnight, the test may
be repeated and/or confirmed with urine that is
collected over a 24-hour period (24-hour urine).
 The quantity of albumin in urine is 30-300 mg/day
in micro albuminuria.
 It is an early indication of nephropathy in diabetic
patients and hypertensive patients.
 It is also expressed as albumin creatinine ratio
 Albumin creatinine ratio – 30-300mg albumin /gm
of creatinine -0.03- 0.3

27.  Over flow proteinuria
 Hemoglobinuria in hemolytic diseases
 Myoglobinuria in muscle crush injury
 Bence- Jones protein in multiple myeloma
 Tubular proteinuria
 Due to decreased functional nephrons the
remaining nephrons over-work .
 Tubular reabsorption of proteins is
impaired causing tubular proteinuria.
 Proximal renal tubular damage -increased
excretion of β- microglobulin.

28.  Urine specific gravity
 Normal value is 1.016-1.022
 Fixed specific gravity at 1.010 is seen in chronic
kidney disease due to tubular dysfunction.
 Specific gravity is increased in diabetes
mellitus, adrenal insufficiency
 Specific gravity is decreased in diabetes
insipidus (ADH insufficiency).

29.  Normal range is 400-850 mOsm/kg
 Plasma osmolality is 285-295 mOsm/kg.
 Normally the urine- plasma osmolality ratio is
above 1.3.
 Urine osmolality - decreased in diabetes insipidus
and the ratio is lesser than 1.
 Concentrating ability of distal tubules and
collecting ducts decreased.
 Due to renal defect (nephrogenic diabetes
insipidus which do not response to ADH )
 Central diabetes insipidus due to ADH deficiency.

31.  For the test, patient should stop taking any fluid for
8 hours (Caution - dehydration).
 In normal subjects urine osmolality will be more
than 800 mOsm/kg, plasma osmolality should not
exceed 295 mOsm/kg and the ratio will be more
than 2.
 In diabetes insipidus, the ratio is below 1 (0.2-0.7).
 water deprivation with ADH stimulation, urine
osmolality - more than 800mOsm/kg - central
D.Insipidus but in nephrogenic diabetes
insipidus,less than 300mOsm/kg.

32.  Urine dilution test
 The patient is not allowed to drink any
fluid after mid night.
 Bladder is emptied at 7 am and water load
(1200 ml over the next 30 min) is given.
 Hourly urine samples are collected for
next four hours.
 The specific gravity of at least one sample
should fall to 1.003 and osmolality to 50
mOsm/kg.
 This test is more sensitive and less harmful
than concentration test.

33.  Enteric coated capsules containing ammonium
chloride at a dose of 0.1 g / kg body wt is given.
 In the liver, NH3 is converted to urea and HCl is
produced which is excreted by kidney.
 Urine is collected hourly from 2 to 8 hours after
ingestion.
 At least one sample should have a pH of 5.3 .
 In type I distal renal tubular acidosis, urinary pH
rarely falls below 6 and never falls below 5.3.

34.  Phenolsulfonthalein test dye 6 mg in 1 ml
saline is given intravenously and urine samples
are collected at 15, 30, 60, 120 minutes.
 If 15 minute urine contains 25% or more, the
test is normal.
 If it is less than 23%, it indicates impaired
renal excretory function.
 Normally 40-60% in 1 hour and 20-25% in the
second hour excreted.

35.  More than 2500 ml / 24 hours it is called polyuria.
 It can be due to : 1. Increase in water loss due to
either diminished tubular dysfunction with
decreased concentration ability or Anti diuretic
hormone deficiency. In ADH deficiency ( diabetes
insipidus), Urine specific gravity will be lowered
 2. Due to excessive solute loss and osmotic diuresis -
In case of diabetic mellitus due to glucosuria, there
will be polyuria. In this case urine specific gravity
will be increased

36. Color of the urine
 Normally it is pale yellow or
amber color.
 Hematuria or hemoglobinuria
produce a dark brown color.
 By microscopy, hematuria can be
detected by the presence of
intact red blood cells.
 It can be due to stone, tumor,
injury, infection.

37. pH of urine
 usually acidic- pH 6 (4.5-8-pH )
Specific gravity
 normally varies from 1.016 to
1.032
Osmolality
 On average fluid intake, 300-900
mOsm/kg
Odor foul smell indicates
bacterial infection

38.  When urine output is lesser then 400 ml /
24 hours, it is called oliguria. If no urine is
passed, then, it is called anuria.
 Oliguria, anuria can be the result of
following:
 Diminished perfusion of kidney ( cardiac
failure, Hypotension, shock),
 Renal disease such as acute
glomerulonephritis, tubular necrosis,
 Obstruction to the outflow e.g. bilateral
tumor in the bladder, renal stones,
prostate enlargement etc.

39. Urine investigations
 Protein , Blood , Sugar, pH ,
specific gravity, osmolality,
Blood investigations
 Blood urea – 15 – 40 mg/dl
 Serum creatinine – 0.6 – 1.2
mg/dl
 Serum uric acid - 4- 7 mg/dl in
men , 3-6 mg/dL in women

40.  Serum sodium - 135- 145 mmol/l
 Serum potassium – 3.5 – 5 mmol/l
 Serum chloride- 96 – 106 mmol/l
 Serum bicarbonate - 23 – 27
mmol/l
 Arterial blood pH - 7.35 – 7.45
 paCO2 - 35 – 45 mm of Hg
 paO2 - 80-100 mm of Hg

44.  A daily phosphate excretion of <3.2 mmol (100 mg)
and a fractional excretion of phosphate <5% (normal
value is 15–20%) allow diagnosis of non-
renal phosphate loss.
 A urinary phosphate excretion >3.2 mmol (100 mg) or
a fractional excretion >5% is indicative of renal
phosphate wasting.
 Fractional excretion of phosphate (FeP) is associated
with end-stage renal disease patients with CKD 3b
and 5
 FGF23 (Phosphotonin)is secreted from bones and acts
on the kidneys to induce phosphaturia and suppress
active vitamin D synthesis
 It maintain phosphate homeostasis.

45.  Decreased in X-linked hypophosphatemic
rickets
 Osteogenic osteomalaia - (failure of
inactivation of phosphatonin)
 Hyperparathyroidism
 Increased in hypoparathyroidism
 Reduced phosphate reabsorption in
hypercalciuric stone, renal tubular dysfunction

46.  Metabolized in tubules
 Urinary measurement of beta 2
microglobulin provide a sensitive index
of assessing tubular integrity
 beta2 microglobulin in urine is
0-0.3 µg/mL.
 In serum or plasma samples is
0-3 µg/mL.

47.  Fractional excretion of Na
= Urine Na / serum Na x 100
Urine creatinine / Sr.creatinine
< 1% in prerenal azotemia
> 1.5% in acute tubular necrosis
> 3% in postrenal failure

48.  RFI (mmol/L) =
 Urine Na x serum creatinine
/ urine creatinine
Prerenal failure < 1 mmol/L
Post renal failure and nephrotoxic
renal failure > 3 mmol/L

49.  Urea , creatinine , uric acid , creatinine
clearance, eGFR, cystatin
 Sodium, potassium, bicarbonate, chloride
 Calcium, phosphorus
 Plasma glucose
 Serum albumin ,total protein
 CBC and Urinalysis
 Urine albumin creatinine ratio
 Beta 2microglobulin
 Sr. AST, ALT ALP, bilirubin

50. Thank you