This document discusses the history and principles of rambo preservation. Rambo preservation was first documented in 1663 and the use of glycerol as a cryoprotectant in 1949 has had a major impact, particularly in reproductive medicine. Rambo preservation involves freezing, storing, and thawing biological materials. It allows for the availability and preservation of raw materials, which is important for technologies like artificial insemination that rely on cryopreserved sperm.