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The document discusses the history and principles of sperm preservation. It notes that the first documented preservation was by Boyle in 1663, while the first concept of sperm preservation was by Lazzaro Spallanzini in 1760. The most significant discovery was the use of glycerol as a cryoprotectant by Polge and Smith in 1949. The ability of glycerol to protect cells from freezing injury was discovered accidentally, and the development of preservation techniques since then have had a huge impact in fields like reproductive medicine.






