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Presentation_Related to Medical Lab Technology.pptx
1.
2. TABLE OF CONTENTS
Introduction
Aims and Objective
Methodology
Data collection procedure
Data analysis procedure
Results
Conclusion
References
3. MOTIVATION
Malaria is one of the major health concerns in Pakistan.
Increased prevalence in Pakistan particularly in Khyber Pakhtunkhwa.
P. vivax responsible for approximately 80% of infections and P. falciparum
causing 20% of infections are the two prevalent Plasmodium species in Pakistan
(Khan et al., 2023).
For this purpose, this research was carried to get insights into the prevalence of
malaria.
4. INTRODUCTION
Malaria is an infectious and deadly parasitic disease caused by
the genus Plasmodium.
Transmitted to human beings through a bite of an infected
Anopheles mosquito. (Djihinto et al., 2022). .
One of the leading causes of death over the world with almost
400,000 deaths per year.
More than 200 million cases of malaria are reported every year
worldwide (WHO 2019).
5. AIMS AND OBJECTIVE
The aim of the current study was to
assess the prevalence of malarial
parasite in district Peshawar.
To evaluate the seasonal malarial infections during
Summer season.
There has been seasonal, age and gender wise
differences in the prevalence of Malaria.
6. Study design:
A cross-sectional descriptive study.
Study setting:
Study was conducted at HMC Lab Hayatabad Medical
Complex Peshawar
Study duration:
This study was conducted after approval of synopsis.
METHODOLOGY
7. METHODOLOGY
Sample size:
Error Margin of 5%
Confidence interval 95%
Prevalence at 23%
Sample size was calculated to be 100 (WHO calculator)
Study Technique:
Non-probability convenient sampling technique.
8. DATA COLLECTION PROCEDURE
100 blood samples were collected from various private labs at Dabgari
gardens and other health care labs in district Peshawar.
10 μL blood drop was poured in strip’s blood region.
2-3 drops of malaria buffer was added to the blood on strip.
Waited for 20 minutes (Abbas and Dijkstra 2020).
RDT and ICT method was used.
9. DATAANALYSIS AND RESULTS
Result interpretation.
Analysis on SPSS (26 version).
Descriptive analysis (frequency and percentages).
Gender, Age and Month wise distribution.
13. CONCLUSION
It was concluded from the current study that Malaria has mostly infected
male patients.
Furthermore, Malaria infection was noted in the month of September and
October.
That may be due to the higher risk of Mosquito nourishment caused by
higher temperatures in this season.
.
14. REFERENCES
1. Djihinto, O.Y., Medjigbodo, A.A., Gangbadja, A.R., Saizonou, H.M., Lagnika, H.O., Nanmede,
D., Djossou, L., Bohounton, R., Sovegnon, P.M., Fanou, M.J. and Agonhossou, R., 2022. Malaria-
Transmitting Vectors Microbiota: Overview and Interactions With Anopheles Mosquito
Biology. Frontiers in Microbiology, 13.
2. World (2019). The ‘World malaria report 2019’ at a glance. [online] Who.int. Available at:
https://www.who.int/news-room/feature-stories/detail/world-malaria-report-2019 [Accessed 12 May
2023].
3. Khan, M.I., Qureshi, H., Bae, S.J., Khattak, A.A., Anwar, M.S., Ahmad, S., Hassan, F. and
Ahmad, S., 2023. Malaria prevalence in Pakistan: A systematic review and meta-analysis (2006–
2021). Heliyon.
4. Abbas, S.S. and Dijkstra, T.M., 2020. Detection and stage classification of Plasmodium
falciparum from images of Giemsa stained thin blood films using random forest classifiers.
Diagnostic pathology, 15(1), pp.1-11.
According to the WHO 2013 malaria report, the prevalence of P. vivax and P. falciparum was 88% and 12% respectively in Pakistan. In the past few decades, malaria transmission has been highest in the northern part of Pakistan, especially in Khyber Pakhtunkhwa (KPK) province (Najeeb et al., 2018). P. vivax responsible for approximately 80% of infections and P. falciparum causing 20% of infections are the two prevalent Plasmodium species in Pakistan (Khan et al., 2020). Currently, there are proven and effective tools to fight malaria, including vector control measures. As these tools are scaled up, malaria-endemic countries need to continually update the skills and competence of the health workers engaged in malaria control and elimination.
Malaria is an infectious and deadly parasitic disease caused by the genus Plasmodium, transmitted to human beings through a bite of an infected mosquito of the Anopheles genus and this disease is associated with fever, anemia and other ailments as stated by Djihinto et al., (2022). Malaria is one of the leading causes of death over the world with almost 400,000 deaths per year. More than 200 million cases of malaria are reported every year worldwide (WHO 2019). Many countries around the world were listed malaria risk area and according to the latest estimates from the WHO there were 214 million new cases of malaria with a record of 438,000 malaria deaths globally (WHO 2016).
The aim of the current study was to assess the prevalence of malarial parasite in district Peshawar and objectives were to; evaluate the seasonal malarial infections during Summer season and to assess the malarial infection in common age and gender factor.
There has been seasonal wise, age wise and gender wise differences in the prevalence of Malaria.
Therefore the focus of this study is to analyze the extent of differences in the prevalence of malaria among population of Khyber Pakhtunkhwa on the basis of season and genders.
The methodology designed for this study is experimental as the samples were collected and analyzed in laboratory settings by using standard protocols.
This study was conducted at HMC Lab Hayatabad Medical Complex Peshawar.
A total of 100 blood samples were collected from suspected patients of malaria infection. Sample collection: At least 2ml of was collected in Ethylene diamine tetra acetic acid (EDTA) tubes through aseptic technique.
Samples were collected from various private labs at Dabgari gardens and other health care labs in district Peshawar. Time duration: This study was conducted in the spring season Sep, 2022 and October, 2022.
This study was done through triplicate test. First of all, RDT or ICT was carried out from all the samples the day they were received at HMC Hayatabad Medical Complex Peshawar. Thick and thin film of blood smear was Giemsa-stained, stored in a secured slide box and was reconfirmed by microscopy (Syed et al., 2020).
Rapid diagnostic test is a device that detects malaria antigen in a small amount of blood, usually 10 μL blood drop was poured in strip’s blood region with 2-3 drops of malaria buffer was added to the blood on strip and waited for 20 minutes for positive and control line (Abbas and Dijkstra 2020).
This figure depicts that out of 100, 36 samples were positive for the malaria. And out of these 36, 24 cases were of males and 12 were of females
The age wise distribution shows that the disease is more prevalent among the age group of 11-20 years old. Whereas the population of 50 years above is least affected.