Seminario biología molecular

1. Ana María Vega Cardona
Tercer semestre
Universidad Pontificia Bolivariana

2. miR-29a
It may be a key pathogenic regulator of periodontits
What is miR-29a?
Is a micro RNA, that protect human gingival
fibroblasts (HGFs) from liposacride-induced
pyrolysis.
MIR-29A
MIR-29A
Introduction

3. The production of:
collagen
elastin
structural proteins
What is the function of fibroblasts?
Fibrolasts injury
What is Fibrolasts injury? It is damage or injury to the fibroblasts
Introduction

4. General Objective
TO INVESTIGATE CIRC_0036490 AND DKK1 BINDING
COMPETITIVELY TO MIR-29A IN PROMOTING
LIPOPOLYSACCHARIDE-INDUCED INJURY OF HUMAN GINGIVAL
FIBROBLASTS

5. Enzyme-linked immunosorbent assay (ELISA)
Reverse transcription quantitative real-
time PCR (RT-qPCR)
Methods
Detects and quantifies specific molecules, such as proteins, peptides,
antibodies, hormones, among others,
The levels of cytokines IL-1β and IL-18 were measured in the supernatant
of human gingival fibroblasts (HGFs) after centrifugation.
It detects and quantifies gene expression through amplification of cDNA
previously obtained by RNA reverse transcription.
Was used to analyze circ_0036490 and DKK1 expression in human
gingival fibroblasts (HGFs) after transfection with specific vectors and
sequences.

6. Flow cytometry assay Western blotting assay
Methods
detects and quantifies specific proteins in a
biological sample by separating the proteins by
gel electrophoresis.
It was used to detect the expression of pro-
caspase-1, cleaved caspase-1 and GSDMD-N
proteins in human gingival fibroblasts (HGFs).
Detects and quantifies different properties of
individual cells, using fluorochrome-labeled
antibodies and detection of the fluorescent
signal generated by the analyzed cells.
It was used to detect cell pyroptosis

7. Results

8. Results

9. Author's name What he said Agree or disagree
Discussion
Zhao XQ, Ao CB, Yan YT.
CircLRP6 antagonised the function
of miR-145a-5p to promote
cementum formation
Agree
Agree
Agree
Zhang F, Cao K, Du G, et al.
DKK1 inhibited miR-29a expression
which was induced by the Wnt
signalling in osteoblasts
Wang P, Li Y, Meng T, et al.
Besides, miR-29a promotes osteoblast
proliferation by downregulating DKK1
expression and activatingWnt/β-catenin
signalling pathway

10. Molecular biology has not only
helped us to understand
molecular mechanisms, it has
allowed us to develop therapies
where defective genes can be
modified to treat hereditary
diseases, such as muscular
dystrophy or cystic fibrosis, and it
has also allowed us to discover
underlying causes of diseases,
such as genes associated with
cancer, guiding research and the
development of specific
treatments.
conclusions
Molecular biology has helped
us to understand how genes
affect the response to drugs,
allowing the personalization of
pharmacological treatments
according to the genetic
profile of a patient, and has
also allowed us to develop
vaccines, hormones, peptides
and drugs, thanks to the
manipulation of genes in
bacteria and cell cultures.

12. THANKS