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2. El microscopio (del griego μικρός micrós, ‘p
equeño’, y σκοπέω scopéo, ‘mirar’)1 es una
herramienta que permite observar objetos, q
ue son demasiado pequeños para ser obser
vados a simple vista. El tipo más común y el
primero que fue inventado es el microscopio
óptico. Se trata de un instrumento que contie
ne dos lentes que permiten obtener una ima
gen aumentada del objeto y que funciona po
r refracción. La ciencia que investiga los obje
tos pequeños utilizando este instrumento se l
lama microscopía.
Hay muchos tipos de microscopios, y puede
n agruparse de diferentes maneras. Una de
ellas es describir el método que utiliza un ins
trumento para interactuar con una muestra y
producir imágenes, ya sea enviando un haz
de luz o electrones a través de una muestra
en su trayectoria óptica, detectando las emisi
ones de fotones de una muestra, o escanea
ndo a través y a corta distancia de la superfi
cie de una muestra utilizando una sonda. El
microscopio más común (y el primero que se
inventó) es el microscopio óptico, que utiliza
lentes para refractar la luz visible que pasa a
través de una muestra finamente seccionada
para producir una imagen observable. Otros
tipos importantes de microscopios son el mic
roscopio de fluorescencia, el microscopio ele
ctrónico (tanto el microscopio electrónico de
transmisión como el de barrido) y varios tipo
s de microscopios de sonda de barrido.2
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