Polymerase chain reaction (PCR) is a technique used to amplify DNA sequences. It was developed in 1984 by Kary Mullis, who won the Nobel Prize in 1993 for this work. PCR uses thermal cycling to amplify a target DNA sequence, allowing for its detection and analysis. It has applications in DNA cloning, sequencing, phylogeny, gene function analysis, diagnosis of hereditary diseases, genetic fingerprinting, paternity testing, and detection of infectious diseases.

1. PCR & ITS APPLICATIONS

3. Polymerase Chain Reaction PCR means Polymerase Chain Reaction It is a technique used to amplify the DNA It was developed in 1984 by Kary Mullis In 1993 Mullis won the Nobel Prize Thermal cycler is an apparatus used to carryout a PCR reaction

4. Polymerase Chain Reaction Minute amount of starting material Quick and simple Extremely sensitive

5. Components required for a PCR reaction Taq DNA polymerases Target sequences Deoxyribonucleoside triphosphates Oligomer primers Amplification buffer Polymerase Chain Reaction

6. Procedure - PCR cycle Polymerase Chain Reaction

7. Procedure - Polymerase Chain reaction Polymerase Chain Reaction

8. Procedure - Polymerase Chain reaction Polymerase Chain Reaction Denaturation Annealing Dna synthesis

9. Procedure - Polymerase Chain reaction Polymerase Chain Reaction Denaturation Annealing Dna synthesis

10. DNA cloning for sequencing DNA-based Phylogeny Functional analysis of Genes The diagnosis of Hereditary diseases The identification of Genetic Fingerprints ( used in forensic sciences and paternity testing ) The detection and diagnosis of Infectious diseases Polymerase Chain Reaction

11. Polymerase Chain Reaction What is PCR? What is the use of PCR? Protocol Applications

12. Polymerase chain reaction Polymerase Chain Reaction

13. Polymerase Chain Reaction

14. Temperature profile of a typical PCR cycle Polymerase Chain Reaction

15. Polymerase Chain Reaction

16. DNA cloning for sequencing DNA-based Phylogeny Functional analysis of Genes The diagnosis of Hereditary diseases The identification of Genetic Fingerprints ( used in forensic sciences and paternity testing ) The detection and diagnosis of Infectious diseases 06/06/09 Polymerase Chain Reaction

17. Temperature profile of a typical PCR cycle Polymerase Chain Reaction