This document provides instructions for making a cut-out shirt from an old shirt. The instructions involve cutting slits and shapes out of the shirt to create an open design, then using iron-on hem tape to reinforce and hem the new edges. The process involves cutting along shoulder seams and button plackets to make an "L" shape, then cutting diagonally to make a triangle out of the "L" shape. An adult helps by ironing the hem tape in place.
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...Allyson Luo
This study aimed to enhance the expression and solubility of the vp13 protein from hepatitis E virus (HEV) by expressing it as a fusion protein with maltose binding protein (MBP) in E. coli. The researchers cloned the ORF3 gene encoding vp13 into a bacterial expression vector with an MBP tag. Initial expression trials found most of the MBP-vp13 fusion protein was insoluble. The researchers then optimized induction conditions, finding induction at 18°C and addition of 1% glucose led to efficient expression of soluble MBP-vp13 fusion protein. This enhanced solubilization and purification of vp13, which will facilitate further study of its structure and function.
A apresentação descreve a solução JMcloud da Dakol para controle remoto de CLPs e IHMs de forma segura e inteligente, reduzindo drasticamente custos de instalação e manutenção. A solução consiste em um servidor centralizado conectado via gateways seguros a dispositivos habilitados, permitindo acesso remoto às IHMs e CLPs. Componentes incluem o servidor JMcloud, aplicativo JMcloud Connect e ativadores de hardware ou software.
Top 15 New Revenue Ideas & Organizational Tips for Event FundraisingLaunch Cause
Join Joe Duca and Joshua Meyer of BidPal in a review of the top 15 ways to supplement standard fundraising activities and ensure your event is as profitable as possible. This workshop will provide the framework for nonprofits to evaluate and enhance their standard fundraising events.
UniStream é a família de CLPs com IHM incorporada de alto desempenho baseada em uma poderosa CPU suportando até 2048 pontos de I/O. A linha apresenta opções de IHM com displays de 7", 10" e 15", diversos tipos de módulos de E/S e comunicação, permitindo assim uma configuração compacta e especifica para sua aplicação, de forma simples e ágil.
Este documento proporciona información sobre la política comercial y el marketing de la empresa PROCOS SL. Explica que la política comercial es importante para conocer los gustos de los clientes y ofrecer un producto de calidad a un precio competitivo. También describe los elementos clave del plan comercial de la empresa, como los objetivos, segmento del mercado, producto, precio, promoción y previsión de ventas. Finalmente, analiza conceptos de marketing como el marketing estratégico y el marketing mix.
Alan Turing was a pioneering computer scientist who made fundamental contributions to artificial intelligence and the field of computer science. He defined the concept of an algorithm and developed the Turing machine, laying the foundations for the digital computer. During World War II, Turing worked at Britain's codebreaking center and cracked the German Enigma cipher, helping the Allies win the war. However, as a gay man in 1950s Britain, Turing was prosecuted for his homosexuality and chemically castrated, which led to his tragic death two years later at the young age of 41. Though he faced great adversity, Turing's revolutionary ideas transformed technology and shaped the modern world.
This document lists information about several museums and archaeological sites in Egypt, including their locations and brief descriptions. It provides web links for the National Museum of Rashid in Rosetta, the Kom el-Shokafa site museum in Alexandria, the Civilization Museum in Kafr El-Sheikh, and several other museums showcasing Egypt's history and archaeological finds from prehistory to today.
O documento discute como as empresas constroem suas identidades, imagens e reputações. A identidade de uma empresa é definida por atributos como nome, logotipo e valores. A imagem é a percepção externa de uma empresa. A reputação é construída ao longo do tempo com base na qualidade das relações com stakeholders. Essas características influenciam a escolha dos consumidores entre empresas semelhantes.
This document provides instructions for making a cut-out shirt from an old shirt. The instructions involve cutting slits and shapes out of the shirt to create an open design, then using iron-on hem tape to reinforce and hem the new edges. The process involves cutting along shoulder seams and button plackets to make an "L" shape, then cutting diagonally to make a triangle out of the "L" shape. An adult helps by ironing the hem tape in place.
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...Allyson Luo
This study aimed to enhance the expression and solubility of the vp13 protein from hepatitis E virus (HEV) by expressing it as a fusion protein with maltose binding protein (MBP) in E. coli. The researchers cloned the ORF3 gene encoding vp13 into a bacterial expression vector with an MBP tag. Initial expression trials found most of the MBP-vp13 fusion protein was insoluble. The researchers then optimized induction conditions, finding induction at 18°C and addition of 1% glucose led to efficient expression of soluble MBP-vp13 fusion protein. This enhanced solubilization and purification of vp13, which will facilitate further study of its structure and function.
A apresentação descreve a solução JMcloud da Dakol para controle remoto de CLPs e IHMs de forma segura e inteligente, reduzindo drasticamente custos de instalação e manutenção. A solução consiste em um servidor centralizado conectado via gateways seguros a dispositivos habilitados, permitindo acesso remoto às IHMs e CLPs. Componentes incluem o servidor JMcloud, aplicativo JMcloud Connect e ativadores de hardware ou software.
Top 15 New Revenue Ideas & Organizational Tips for Event FundraisingLaunch Cause
Join Joe Duca and Joshua Meyer of BidPal in a review of the top 15 ways to supplement standard fundraising activities and ensure your event is as profitable as possible. This workshop will provide the framework for nonprofits to evaluate and enhance their standard fundraising events.
UniStream é a família de CLPs com IHM incorporada de alto desempenho baseada em uma poderosa CPU suportando até 2048 pontos de I/O. A linha apresenta opções de IHM com displays de 7", 10" e 15", diversos tipos de módulos de E/S e comunicação, permitindo assim uma configuração compacta e especifica para sua aplicação, de forma simples e ágil.
Este documento proporciona información sobre la política comercial y el marketing de la empresa PROCOS SL. Explica que la política comercial es importante para conocer los gustos de los clientes y ofrecer un producto de calidad a un precio competitivo. También describe los elementos clave del plan comercial de la empresa, como los objetivos, segmento del mercado, producto, precio, promoción y previsión de ventas. Finalmente, analiza conceptos de marketing como el marketing estratégico y el marketing mix.
Alan Turing was a pioneering computer scientist who made fundamental contributions to artificial intelligence and the field of computer science. He defined the concept of an algorithm and developed the Turing machine, laying the foundations for the digital computer. During World War II, Turing worked at Britain's codebreaking center and cracked the German Enigma cipher, helping the Allies win the war. However, as a gay man in 1950s Britain, Turing was prosecuted for his homosexuality and chemically castrated, which led to his tragic death two years later at the young age of 41. Though he faced great adversity, Turing's revolutionary ideas transformed technology and shaped the modern world.
This document lists information about several museums and archaeological sites in Egypt, including their locations and brief descriptions. It provides web links for the National Museum of Rashid in Rosetta, the Kom el-Shokafa site museum in Alexandria, the Civilization Museum in Kafr El-Sheikh, and several other museums showcasing Egypt's history and archaeological finds from prehistory to today.
O documento discute como as empresas constroem suas identidades, imagens e reputações. A identidade de uma empresa é definida por atributos como nome, logotipo e valores. A imagem é a percepção externa de uma empresa. A reputação é construída ao longo do tempo com base na qualidade das relações com stakeholders. Essas características influenciam a escolha dos consumidores entre empresas semelhantes.
A empresa de tecnologia anunciou um novo smartphone com câmera aprimorada, tela maior e bateria de longa duração por um preço acessível. O dispositivo tem como objetivo atrair mais consumidores em mercados emergentes com suas especificações equilibradas e preço baixo. Analistas esperam que as melhorias e o preço baixo impulsionem as vendas do novo aparelho.
Hplc (basic principles, operation and maintenance)alim125135
This document provides an overview of high performance liquid chromatography (HPLC), including its basic principles, instrumentation, separation modes, detectors, maintenance, troubleshooting, and good laboratory practices. It discusses reversed phase and normal phase HPLC systems. Key topics covered include column configuration, peak parameters, solvent preparation, buffer preparation, filtration, preventative maintenance of individual HPLC modules, common pump and autosampler problems, and replacing worn parts. The document emphasizes correct methodology and explains that proper maintenance can maximize system performance and component lifetimes.