This study aims to assess the function of the Seed Storage Albumin 1 (SESA1) gene in Arabidopsis thaliana. SESA1 has a bifunctional trypsin/α-amylase inhibitory domain and is involved in lipid storage. The splicing activator SR45 has been shown to downregulate SESA1 expression. The researchers found that SESA1 expression is downregulated in the sr45-1 mutant compared to wild type and overexpression lines, as assessed by qPCR. SESA1 was successfully cloned into E. coli using restriction enzymes. The goal is to express the SESA1 protein and test its ability to inhibit α-amylase using a reducing sugar assay