Redox-potenciál mérésen alapuló gyors mikrobiológiai módszerOlivér Reichart
Mikrobiológiai módszerek áttekintése és összehasonlítása, a redox-potenciál alapú technológia bemutatása. Az új módszer előnyei:
* Egyszerű mérési technika.
* Nem igényel szigorú hőmérséklet-szabályozást.
* Gyors módszer, különösen nagy mikroba-számú fertőzések esetében.
* Bármely tápleves alkalmazható (impedimetriás mérések kis vezetőképességű, speciális tápleveseket igényelnek).
* Különösen alkalmas membrán-szűréses módszer kiértékelésére.
* Gazdaságos, hatékony és egyszerű módszer pusztulás-kinetikai mérések kiértékelésére.
* Nagyon hatékony módszer táptalaj-optimalizálási kísérletekhez.
* A vizsgálatok költsége kisebb a klasszikus módszerekhez viszonyítva, különösen null-toleráns mikrobák (coliforms, Enterococcus, Pseudomonas, etc.) meghatározásánál.
MICROTESTER - A redox-potenciál mérésen alapuló gyors vizsgálati módszer elmé...Olivér Reichart
A redox-potenciál mérésen alapuló modszer részletes bemutatása:
* Gyors módszer, különösen nagy mikroba-számú minták esetében.
* Egyszerű mérési technika.
* Szabványos táptalajok használhatók.
* A redox-görbe alakjából következtetni lehet a mikroba-csoportra.
* Nagyon széles (100-108) sejtszám-tartományban hígítás nélkül alkalmazható.
* Különösen célszerű membrán-szűréses módszer kiértékelésére.
* Élelmiszeripari vizsgálatokra validált és akkreditált módszer.
Presentation to the Chicago Data Viz Meetup group in March of 2014. Chicago's bike share program, Divvy, published data on the first 750,000 trips local riders had taken in 2013. As part of the Divvy Data Challenge, I tried to find some meaning to the massive pile of data and had a lot of fun in the process.
While I didn't win anything as part of the challenge, I was invited by the City of Chicago's Chief Data Officer (such a cool title), Tom Schenk, to present my project to a Chicago Data Viz Meetup.
This presentation was originally delivered in Keynote. Alas, Slideshare prefers PDF. For more specific information on this particular project, check out http://drewdepriest.com/divvy.
Redox-potenciál mérésen alapuló gyors mikrobiológiai módszerOlivér Reichart
Mikrobiológiai módszerek áttekintése és összehasonlítása, a redox-potenciál alapú technológia bemutatása. Az új módszer előnyei:
* Egyszerű mérési technika.
* Nem igényel szigorú hőmérséklet-szabályozást.
* Gyors módszer, különösen nagy mikroba-számú fertőzések esetében.
* Bármely tápleves alkalmazható (impedimetriás mérések kis vezetőképességű, speciális tápleveseket igényelnek).
* Különösen alkalmas membrán-szűréses módszer kiértékelésére.
* Gazdaságos, hatékony és egyszerű módszer pusztulás-kinetikai mérések kiértékelésére.
* Nagyon hatékony módszer táptalaj-optimalizálási kísérletekhez.
* A vizsgálatok költsége kisebb a klasszikus módszerekhez viszonyítva, különösen null-toleráns mikrobák (coliforms, Enterococcus, Pseudomonas, etc.) meghatározásánál.
MICROTESTER - A redox-potenciál mérésen alapuló gyors vizsgálati módszer elmé...Olivér Reichart
A redox-potenciál mérésen alapuló modszer részletes bemutatása:
* Gyors módszer, különösen nagy mikroba-számú minták esetében.
* Egyszerű mérési technika.
* Szabványos táptalajok használhatók.
* A redox-görbe alakjából következtetni lehet a mikroba-csoportra.
* Nagyon széles (100-108) sejtszám-tartományban hígítás nélkül alkalmazható.
* Különösen célszerű membrán-szűréses módszer kiértékelésére.
* Élelmiszeripari vizsgálatokra validált és akkreditált módszer.
Presentation to the Chicago Data Viz Meetup group in March of 2014. Chicago's bike share program, Divvy, published data on the first 750,000 trips local riders had taken in 2013. As part of the Divvy Data Challenge, I tried to find some meaning to the massive pile of data and had a lot of fun in the process.
While I didn't win anything as part of the challenge, I was invited by the City of Chicago's Chief Data Officer (such a cool title), Tom Schenk, to present my project to a Chicago Data Viz Meetup.
This presentation was originally delivered in Keynote. Alas, Slideshare prefers PDF. For more specific information on this particular project, check out http://drewdepriest.com/divvy.
2024 State of Marketing Report – by HubspotMarius Sescu
https://www.hubspot.com/state-of-marketing
· Scaling relationships and proving ROI
· Social media is the place for search, sales, and service
· Authentic influencer partnerships fuel brand growth
· The strongest connections happen via call, click, chat, and camera.
· Time saved with AI leads to more creative work
· Seeking: A single source of truth
· TLDR; Get on social, try AI, and align your systems.
· More human marketing, powered by robots
ChatGPT is a revolutionary addition to the world since its introduction in 2022. A big shift in the sector of information gathering and processing happened because of this chatbot. What is the story of ChatGPT? How is the bot responding to prompts and generating contents? Swipe through these slides prepared by Expeed Software, a web development company regarding the development and technical intricacies of ChatGPT!
Product Design Trends in 2024 | Teenage EngineeringsPixeldarts
The realm of product design is a constantly changing environment where technology and style intersect. Every year introduces fresh challenges and exciting trends that mold the future of this captivating art form. In this piece, we delve into the significant trends set to influence the look and functionality of product design in the year 2024.
How Race, Age and Gender Shape Attitudes Towards Mental HealthThinkNow
Mental health has been in the news quite a bit lately. Dozens of U.S. states are currently suing Meta for contributing to the youth mental health crisis by inserting addictive features into their products, while the U.S. Surgeon General is touring the nation to bring awareness to the growing epidemic of loneliness and isolation. The country has endured periods of low national morale, such as in the 1970s when high inflation and the energy crisis worsened public sentiment following the Vietnam War. The current mood, however, feels different. Gallup recently reported that national mental health is at an all-time low, with few bright spots to lift spirits.
To better understand how Americans are feeling and their attitudes towards mental health in general, ThinkNow conducted a nationally representative quantitative survey of 1,500 respondents and found some interesting differences among ethnic, age and gender groups.
Technology
For example, 52% agree that technology and social media have a negative impact on mental health, but when broken out by race, 61% of Whites felt technology had a negative effect, and only 48% of Hispanics thought it did.
While technology has helped us keep in touch with friends and family in faraway places, it appears to have degraded our ability to connect in person. Staying connected online is a double-edged sword since the same news feed that brings us pictures of the grandkids and fluffy kittens also feeds us news about the wars in Israel and Ukraine, the dysfunction in Washington, the latest mass shooting and the climate crisis.
Hispanics may have a built-in defense against the isolation technology breeds, owing to their large, multigenerational households, strong social support systems, and tendency to use social media to stay connected with relatives abroad.
Age and Gender
When asked how individuals rate their mental health, men rate it higher than women by 11 percentage points, and Baby Boomers rank it highest at 83%, saying it’s good or excellent vs. 57% of Gen Z saying the same.
Gen Z spends the most amount of time on social media, so the notion that social media negatively affects mental health appears to be correlated. Unfortunately, Gen Z is also the generation that’s least comfortable discussing mental health concerns with healthcare professionals. Only 40% of them state they’re comfortable discussing their issues with a professional compared to 60% of Millennials and 65% of Boomers.
Race Affects Attitudes
As seen in previous research conducted by ThinkNow, Asian Americans lag other groups when it comes to awareness of mental health issues. Twenty-four percent of Asian Americans believe that having a mental health issue is a sign of weakness compared to the 16% average for all groups. Asians are also considerably less likely to be aware of mental health services in their communities (42% vs. 55%) and most likely to seek out information on social media (51% vs. 35%).
AI Trends in Creative Operations 2024 by Artwork Flow.pdfmarketingartwork
This article is all about what AI trends will emerge in the field of creative operations in 2024. All the marketers and brand builders should be aware of these trends for their further use and save themselves some time!
A report by thenetworkone and Kurio.
The contributing experts and agencies are (in an alphabetical order): Sylwia Rytel, Social Media Supervisor, 180heartbeats + JUNG v MATT (PL), Sharlene Jenner, Vice President - Director of Engagement Strategy, Abelson Taylor (USA), Alex Casanovas, Digital Director, Atrevia (ES), Dora Beilin, Senior Social Strategist, Barrett Hoffher (USA), Min Seo, Campaign Director, Brand New Agency (KR), Deshé M. Gully, Associate Strategist, Day One Agency (USA), Francesca Trevisan, Strategist, Different (IT), Trevor Crossman, CX and Digital Transformation Director; Olivia Hussey, Strategic Planner; Simi Srinarula, Social Media Manager, The Hallway (AUS), James Hebbert, Managing Director, Hylink (CN / UK), Mundy Álvarez, Planning Director; Pedro Rojas, Social Media Manager; Pancho González, CCO, Inbrax (CH), Oana Oprea, Head of Digital Planning, Jam Session Agency (RO), Amy Bottrill, Social Account Director, Launch (UK), Gaby Arriaga, Founder, Leonardo1452 (MX), Shantesh S Row, Creative Director, Liwa (UAE), Rajesh Mehta, Chief Strategy Officer; Dhruv Gaur, Digital Planning Lead; Leonie Mergulhao, Account Supervisor - Social Media & PR, Medulla (IN), Aurelija Plioplytė, Head of Digital & Social, Not Perfect (LI), Daiana Khaidargaliyeva, Account Manager, Osaka Labs (UK / USA), Stefanie Söhnchen, Vice President Digital, PIABO Communications (DE), Elisabeth Winiartati, Managing Consultant, Head of Global Integrated Communications; Lydia Aprina, Account Manager, Integrated Marketing and Communications; Nita Prabowo, Account Manager, Integrated Marketing and Communications; Okhi, Web Developer, PNTR Group (ID), Kei Obusan, Insights Director; Daffi Ranandi, Insights Manager, Radarr (SG), Gautam Reghunath, Co-founder & CEO, Talented (IN), Donagh Humphreys, Head of Social and Digital Innovation, THINKHOUSE (IRE), Sarah Yim, Strategy Director, Zulu Alpha Kilo (CA).
Trends In Paid Search: Navigating The Digital Landscape In 2024Search Engine Journal
The search marketing landscape is evolving rapidly with new technologies, and professionals, like you, rely on innovative paid search strategies to meet changing demands.
It’s important that you’re ready to implement new strategies in 2024.
Check this out and learn the top trends in paid search advertising that are expected to gain traction, so you can drive higher ROI more efficiently in 2024.
You’ll learn:
- The latest trends in AI and automation, and what this means for an evolving paid search ecosystem.
- New developments in privacy and data regulation.
- Emerging ad formats that are expected to make an impact next year.
Watch Sreekant Lanka from iQuanti and Irina Klein from OneMain Financial as they dive into the future of paid search and explore the trends, strategies, and technologies that will shape the search marketing landscape.
If you’re looking to assess your paid search strategy and design an industry-aligned plan for 2024, then this webinar is for you.
5 Public speaking tips from TED - Visualized summarySpeakerHub
From their humble beginnings in 1984, TED has grown into the world’s most powerful amplifier for speakers and thought-leaders to share their ideas. They have over 2,400 filmed talks (not including the 30,000+ TEDx videos) freely available online, and have hosted over 17,500 events around the world.
With over one billion views in a year, it’s no wonder that so many speakers are looking to TED for ideas on how to share their message more effectively.
The article “5 Public-Speaking Tips TED Gives Its Speakers”, by Carmine Gallo for Forbes, gives speakers five practical ways to connect with their audience, and effectively share their ideas on stage.
Whether you are gearing up to get on a TED stage yourself, or just want to master the skills that so many of their speakers possess, these tips and quotes from Chris Anderson, the TED Talks Curator, will encourage you to make the most impactful impression on your audience.
See the full article and more summaries like this on SpeakerHub here: https://speakerhub.com/blog/5-presentation-tips-ted-gives-its-speakers
See the original article on Forbes here:
http://www.forbes.com/forbes/welcome/?toURL=http://www.forbes.com/sites/carminegallo/2016/05/06/5-public-speaking-tips-ted-gives-its-speakers/&refURL=&referrer=#5c07a8221d9b
ChatGPT and the Future of Work - Clark Boyd Clark Boyd
Everyone is in agreement that ChatGPT (and other generative AI tools) will shape the future of work. Yet there is little consensus on exactly how, when, and to what extent this technology will change our world.
Businesses that extract maximum value from ChatGPT will use it as a collaborative tool for everything from brainstorming to technical maintenance.
For individuals, now is the time to pinpoint the skills the future professional will need to thrive in the AI age.
Check out this presentation to understand what ChatGPT is, how it will shape the future of work, and how you can prepare to take advantage.
A brief introduction to DataScience with explaining of the concepts, algorithms, machine learning, supervised and unsupervised learning, clustering, statistics, data preprocessing, real-world applications etc.
It's part of a Data Science Corner Campaign where I will be discussing the fundamentals of DataScience, AIML, Statistics etc.
Time Management & Productivity - Best PracticesVit Horky
Here's my presentation on by proven best practices how to manage your work time effectively and how to improve your productivity. It includes practical tips and how to use tools such as Slack, Google Apps, Hubspot, Google Calendar, Gmail and others.
The six step guide to practical project managementMindGenius
The six step guide to practical project management
If you think managing projects is too difficult, think again.
We’ve stripped back project management processes to the
basics – to make it quicker and easier, without sacrificing
the vital ingredients for success.
“If you’re looking for some real-world guidance, then The Six Step Guide to Practical Project Management will help.”
Dr Andrew Makar, Tactical Project Management
2024 State of Marketing Report – by HubspotMarius Sescu
https://www.hubspot.com/state-of-marketing
· Scaling relationships and proving ROI
· Social media is the place for search, sales, and service
· Authentic influencer partnerships fuel brand growth
· The strongest connections happen via call, click, chat, and camera.
· Time saved with AI leads to more creative work
· Seeking: A single source of truth
· TLDR; Get on social, try AI, and align your systems.
· More human marketing, powered by robots
ChatGPT is a revolutionary addition to the world since its introduction in 2022. A big shift in the sector of information gathering and processing happened because of this chatbot. What is the story of ChatGPT? How is the bot responding to prompts and generating contents? Swipe through these slides prepared by Expeed Software, a web development company regarding the development and technical intricacies of ChatGPT!
Product Design Trends in 2024 | Teenage EngineeringsPixeldarts
The realm of product design is a constantly changing environment where technology and style intersect. Every year introduces fresh challenges and exciting trends that mold the future of this captivating art form. In this piece, we delve into the significant trends set to influence the look and functionality of product design in the year 2024.
How Race, Age and Gender Shape Attitudes Towards Mental HealthThinkNow
Mental health has been in the news quite a bit lately. Dozens of U.S. states are currently suing Meta for contributing to the youth mental health crisis by inserting addictive features into their products, while the U.S. Surgeon General is touring the nation to bring awareness to the growing epidemic of loneliness and isolation. The country has endured periods of low national morale, such as in the 1970s when high inflation and the energy crisis worsened public sentiment following the Vietnam War. The current mood, however, feels different. Gallup recently reported that national mental health is at an all-time low, with few bright spots to lift spirits.
To better understand how Americans are feeling and their attitudes towards mental health in general, ThinkNow conducted a nationally representative quantitative survey of 1,500 respondents and found some interesting differences among ethnic, age and gender groups.
Technology
For example, 52% agree that technology and social media have a negative impact on mental health, but when broken out by race, 61% of Whites felt technology had a negative effect, and only 48% of Hispanics thought it did.
While technology has helped us keep in touch with friends and family in faraway places, it appears to have degraded our ability to connect in person. Staying connected online is a double-edged sword since the same news feed that brings us pictures of the grandkids and fluffy kittens also feeds us news about the wars in Israel and Ukraine, the dysfunction in Washington, the latest mass shooting and the climate crisis.
Hispanics may have a built-in defense against the isolation technology breeds, owing to their large, multigenerational households, strong social support systems, and tendency to use social media to stay connected with relatives abroad.
Age and Gender
When asked how individuals rate their mental health, men rate it higher than women by 11 percentage points, and Baby Boomers rank it highest at 83%, saying it’s good or excellent vs. 57% of Gen Z saying the same.
Gen Z spends the most amount of time on social media, so the notion that social media negatively affects mental health appears to be correlated. Unfortunately, Gen Z is also the generation that’s least comfortable discussing mental health concerns with healthcare professionals. Only 40% of them state they’re comfortable discussing their issues with a professional compared to 60% of Millennials and 65% of Boomers.
Race Affects Attitudes
As seen in previous research conducted by ThinkNow, Asian Americans lag other groups when it comes to awareness of mental health issues. Twenty-four percent of Asian Americans believe that having a mental health issue is a sign of weakness compared to the 16% average for all groups. Asians are also considerably less likely to be aware of mental health services in their communities (42% vs. 55%) and most likely to seek out information on social media (51% vs. 35%).
AI Trends in Creative Operations 2024 by Artwork Flow.pdfmarketingartwork
This article is all about what AI trends will emerge in the field of creative operations in 2024. All the marketers and brand builders should be aware of these trends for their further use and save themselves some time!
A report by thenetworkone and Kurio.
The contributing experts and agencies are (in an alphabetical order): Sylwia Rytel, Social Media Supervisor, 180heartbeats + JUNG v MATT (PL), Sharlene Jenner, Vice President - Director of Engagement Strategy, Abelson Taylor (USA), Alex Casanovas, Digital Director, Atrevia (ES), Dora Beilin, Senior Social Strategist, Barrett Hoffher (USA), Min Seo, Campaign Director, Brand New Agency (KR), Deshé M. Gully, Associate Strategist, Day One Agency (USA), Francesca Trevisan, Strategist, Different (IT), Trevor Crossman, CX and Digital Transformation Director; Olivia Hussey, Strategic Planner; Simi Srinarula, Social Media Manager, The Hallway (AUS), James Hebbert, Managing Director, Hylink (CN / UK), Mundy Álvarez, Planning Director; Pedro Rojas, Social Media Manager; Pancho González, CCO, Inbrax (CH), Oana Oprea, Head of Digital Planning, Jam Session Agency (RO), Amy Bottrill, Social Account Director, Launch (UK), Gaby Arriaga, Founder, Leonardo1452 (MX), Shantesh S Row, Creative Director, Liwa (UAE), Rajesh Mehta, Chief Strategy Officer; Dhruv Gaur, Digital Planning Lead; Leonie Mergulhao, Account Supervisor - Social Media & PR, Medulla (IN), Aurelija Plioplytė, Head of Digital & Social, Not Perfect (LI), Daiana Khaidargaliyeva, Account Manager, Osaka Labs (UK / USA), Stefanie Söhnchen, Vice President Digital, PIABO Communications (DE), Elisabeth Winiartati, Managing Consultant, Head of Global Integrated Communications; Lydia Aprina, Account Manager, Integrated Marketing and Communications; Nita Prabowo, Account Manager, Integrated Marketing and Communications; Okhi, Web Developer, PNTR Group (ID), Kei Obusan, Insights Director; Daffi Ranandi, Insights Manager, Radarr (SG), Gautam Reghunath, Co-founder & CEO, Talented (IN), Donagh Humphreys, Head of Social and Digital Innovation, THINKHOUSE (IRE), Sarah Yim, Strategy Director, Zulu Alpha Kilo (CA).
Trends In Paid Search: Navigating The Digital Landscape In 2024Search Engine Journal
The search marketing landscape is evolving rapidly with new technologies, and professionals, like you, rely on innovative paid search strategies to meet changing demands.
It’s important that you’re ready to implement new strategies in 2024.
Check this out and learn the top trends in paid search advertising that are expected to gain traction, so you can drive higher ROI more efficiently in 2024.
You’ll learn:
- The latest trends in AI and automation, and what this means for an evolving paid search ecosystem.
- New developments in privacy and data regulation.
- Emerging ad formats that are expected to make an impact next year.
Watch Sreekant Lanka from iQuanti and Irina Klein from OneMain Financial as they dive into the future of paid search and explore the trends, strategies, and technologies that will shape the search marketing landscape.
If you’re looking to assess your paid search strategy and design an industry-aligned plan for 2024, then this webinar is for you.
5 Public speaking tips from TED - Visualized summarySpeakerHub
From their humble beginnings in 1984, TED has grown into the world’s most powerful amplifier for speakers and thought-leaders to share their ideas. They have over 2,400 filmed talks (not including the 30,000+ TEDx videos) freely available online, and have hosted over 17,500 events around the world.
With over one billion views in a year, it’s no wonder that so many speakers are looking to TED for ideas on how to share their message more effectively.
The article “5 Public-Speaking Tips TED Gives Its Speakers”, by Carmine Gallo for Forbes, gives speakers five practical ways to connect with their audience, and effectively share their ideas on stage.
Whether you are gearing up to get on a TED stage yourself, or just want to master the skills that so many of their speakers possess, these tips and quotes from Chris Anderson, the TED Talks Curator, will encourage you to make the most impactful impression on your audience.
See the full article and more summaries like this on SpeakerHub here: https://speakerhub.com/blog/5-presentation-tips-ted-gives-its-speakers
See the original article on Forbes here:
http://www.forbes.com/forbes/welcome/?toURL=http://www.forbes.com/sites/carminegallo/2016/05/06/5-public-speaking-tips-ted-gives-its-speakers/&refURL=&referrer=#5c07a8221d9b
ChatGPT and the Future of Work - Clark Boyd Clark Boyd
Everyone is in agreement that ChatGPT (and other generative AI tools) will shape the future of work. Yet there is little consensus on exactly how, when, and to what extent this technology will change our world.
Businesses that extract maximum value from ChatGPT will use it as a collaborative tool for everything from brainstorming to technical maintenance.
For individuals, now is the time to pinpoint the skills the future professional will need to thrive in the AI age.
Check out this presentation to understand what ChatGPT is, how it will shape the future of work, and how you can prepare to take advantage.
A brief introduction to DataScience with explaining of the concepts, algorithms, machine learning, supervised and unsupervised learning, clustering, statistics, data preprocessing, real-world applications etc.
It's part of a Data Science Corner Campaign where I will be discussing the fundamentals of DataScience, AIML, Statistics etc.
Time Management & Productivity - Best PracticesVit Horky
Here's my presentation on by proven best practices how to manage your work time effectively and how to improve your productivity. It includes practical tips and how to use tools such as Slack, Google Apps, Hubspot, Google Calendar, Gmail and others.
The six step guide to practical project managementMindGenius
The six step guide to practical project management
If you think managing projects is too difficult, think again.
We’ve stripped back project management processes to the
basics – to make it quicker and easier, without sacrificing
the vital ingredients for success.
“If you’re looking for some real-world guidance, then The Six Step Guide to Practical Project Management will help.”
Dr Andrew Makar, Tactical Project Management
8. 8
Microtester Mini készülék alkalmazása:
• A készülék a termelőhöz kihelyezhető
• Kezelése viszonylag egyszerű
• Az adatok a tejbegyűjtés időpontjában leolvashatók
NYERSTEJ VIZSGÁLATA KÖZVETLENÜL A
TEJTERMELŐNÉL
14. • MicroTester készülékkel a Salmonellák RVS
tápoldatban kimutathatók.
• Elődúsítás nem szükséges.
• A zavaró mikroflóra az RVS-ben nem szaporodik.
• Élelmiszerek (tej, sajt, stb.) a tápoldathoz adása a
kimutatási időt csökkenti, de a szelektivitást nem
befolyásolja.
• A Salmonella jelenlétének igazolása a
MicroTesterrel végzett mérés után, a gyanús
mintákból PCR-al elvégezhető.
SALMONELLA KIMUTATÁSA
15. LISTERIÁK KIMUTATÁSA
Listeriák redox görbéi (1/2 Fraser, 37 °C)
-400
-300
-200
-100
0
100
200
300
400
500
0 5 10 15 20 25
t (h)
Eh(mV)
L. monocytogenes L. innocua L. ivanovi
17. • Fraser levesben a listeriák jelenléte a szokásos
módszernél gyorsabban kimutatható.
• A táptalaj szelektivitását a hozzáadott élelmiszer
(tej) nem befolyásolja.
• A tejben leggyakoribb zavaró mikroba, az E. coli a
tápoldatban nem szaporodik.
• A Listeria monocytogenes jelenléte csak további
vizsgálatokkal (PCR) igazolható.
LISTERIA MONOCYTOGENES KIMUTATÁSA
25. • Üdítőitalok penész- és élesztőgomba
számának meghatározása:
– Indirekt mérés (a szaporodás során képződő CO2-ot KOH
oldatban nyeletjük el és a KOH oldat redox-potenciál
változását mérjük)
MINŐSÉGELLENŐRZÉS
ÜDÍTŐITALOK
29. • Ivóvíz mikrobiológiai szennyezettségének gyors
kimutatása
• Ásványvíz mikrobiológiai gyártásközi
minőségellenőrzése
• Kezelt víz (pl. ioncserélt víz, R.O. víz, stb.) gyors
mikrobiológiai vizsgálata
• Fürdővizek mikrobiológiai állapotának gyors
vizsgálata
• Szennyvizek gyors mikrobiológiai vizsgálata
MINŐSÉGELLENŐRZÉS
VÍZ
31. 31
ÁSVÁNYVÍZ VIZSGÁLATOK EREDMÉNYEI
Mikroba
Egy sejt kimutatásához szükséges
vizsgálati idő (h)
Escherichia coli 11
Citrobacter freundii 23
Pseudomonas aeruginosa 24
Enterococcus faecalis 15
32. 32
ÜZEMI ELLENŐRZŐ VIZSGÁLATOK 1.
TYLICZ (TÉTELMINŐSÍTŐ VIZSGÁLAT)
Egy membránon 3 x 250 ml-t szűrtünk, egy mérőcellába 4 membránt
tettünk (egy mérőcellában egyszerre 12 palack minőségét ellenőriztük)
Palackok
sorszáma 1-12. 13-24. 25-36. 37-48. 49-60. 61-72.
Laboratóriumi
mérés
eredménye
negatív negatív negatív negatív negatív negatív
Microtester
mérés
eredménye
negatív negatív negatív negatív negatív negatív
33. 33
ÜZEMI ELLENŐRZŐ VIZSGÁLATOK 2.
(Zalaszentgrót, Hoffmanné & Tar-Géri, 2008)
Vizsgált
mikroba
Összes
mérés
(db)
Egyezés a
szabványos
vizsgálattal (%)
Fals pozitív
eredmény
(%)
Fals negatív
eredmény
(%)
Escherichia coli 942 99,89 0,11 0,00
Coliform 4674 99,87 0,00 0,13
Enterococcus 3000 99,93 0,00 0,07
Pseudomonas
aeruginosa
3372 99,82 0,06 0,12
34. TERMÉSZETES FÜRDŐVÍZ
Velencei tó (Agárd strand)
y = -0.8416x + 11.565
R2
= 0.9871
y = -0.299x + 4.7342
R2
= 0.9917
-1
0
1
2
3
0 5 10 15 20
TTD (h)
lgN(cfu/100ml)
Enterococcus E.coli
48. • Talajba került vegyszerek talaj-mikroflóra
aktivitását befolyásoló hatásának vizsgálata
– Például antibiotikumok hatása különböző típusú talajok
mikrobiális aktivitására
KÖRNYEZETVÉDELEM – BAKTÉRIUMOK
AKTIVITÁSÁNAK VIZSGÁLATA
49. 49
Doxycyclin, talaj
y = 8.8762x - 11.612
y = 6.5508x - 8.295
y = 4.4082x - 0.8414
y = 13.362x - 20.614
y = 2.0662x + 4.0359
0
5
10
15
20
25
2.0 2.5 3.0 3.5
lgc (mg/kg)
TTD(h)
T1 T2 T3 T4 T5
ANTIBIOTIKUM MIKROBA AKTIVITÁST CSÖKKENTŐ
HATÁSA KÜLÖNBÖZŐ TALAJOKBAN
51. 51
• Egyszerű mérési technika
• Nem igényel speciális mérőcellákat
• Nincs szükség szigorú hőmérséklet-szabályozásra
– 0,5 °C pontosságú laboratóriumi termosztátok alkalmasak
– (Az impedimetriás technika 0,002 °C pontosságú szabályozást igényel)
• Nincs szükség speciális táptalajokra
– Alkalmazhatóak a szabványos, illetve egyéb táptalajok
– (Az impedimetriás technika speciális tápleveseket igényel)
A MICROTESTER ELŐNYEI 1.
52. 52
• Nincs szükség a minta hígítására
– 100-108 cfu/mérőcella tartományban a minta hígítás nélkül mérhető
• Alkalmas membránszűréssel koncentrált, illetve
tamponos minták mikrobaszámának hígítás nélküli,
közvetlen meghatározására
– Egyetlen mérőcellába több membránszűrő is behelyezhető.
– Egyetlen mérőcella eredménye akár több liter mintát reprezentálhat
• Ásványvizek tétel-minősítése
• Vízügyi haváriák gyors kimutatása
• Direkt mérésnél eredmény általában néhány órán
belül
– Tenyésztéses vizsgálatok időigénye néhány nap
A MICROTESTER ELŐNYEI 2.
53. 53
• Gyors és egyszerű módszer a szaporodás- és
pusztulás-kinetikai, valamint táptalaj-optimalizálási
vizsgálatokhoz
• A módszer kellően szelektív
– Különös előnyt jelent, hogy a táptalaj szelektivitásán túl a redox-görbe
alakja is jellemző a szaporodó mikroba-csoportra.
– Ezt a szelektivitást a PCR technikán kívül egyéb módszerek nem érik el
A MICROTESTER ELŐNYEI 3.
54. 54
• A nagy mintamennyiségből kiinduló szelektív dúsítás
révén a MicroTester kiválóan alkalmazható a PCR-
technika elődúsító lépéseként
– A célmikrobát tartalmazó mikrobacsoportra negatív mintát nem
érdemes PCR-ral tovább vizsgálni
– A mikrobacsoportra pozitív dúsítás PCR-ral közvetlenül vizsgálható, a
mikrobaszám jelentősen meghaladja a 106/ml koncentrációt
– Csak a pozitivitás esélyével bíró minták kerülnek PCR vizsgálatra, ami
jelentős megtakarítást eredményez
• A minta-mennyiségre vonatkoztatott fajlagos vizsgálati
költség jelentősen alacsonyabb a tenyésztéses,
valamint az impedimetriás gyorsmódszerek
költségénél
A MICROTESTER ELŐNYEI 4.