CMP Degree College
(AConstituent PG College of University of Allahabad)
Topic: Microbiology of fermented
foods
Presented to:
Dr.Avinash singh
Presented by:
Nitesh Kumar Yadav
M.Sc. (Botany) III Semester
DEPARTMENT OF BOTANY
2.
Historical Background ofFermentation
• Fermentation is one of the oldest food preservation techniques, used
for over 8000 years.
• Historical records show beer fermentation in Mesopotamia (6000
BC) and bread-making in Egypt (4000 BC).
• In India, traditional products like dahi, idli, dosa, gundruk have been
fermented for centuries.
• Pasteur (1857) first demonstrated that microorganisms were
responsible for fermentation, not just chemical processes.
• This discovery led to the foundation of industrial microbiology.
3.
Definition and Importance
•Fermentation: Anaerobic breakdown of carbohydrates by
microorganisms into simpler end-products like lactic acid, ethanol,
CO₂, etc.
• Enhances:
◦Shelf-life
◦Nutritional value
◦Digestibility
◦Flavor and texture
◦Safety by inhibiting pathogens
• Fermentation is now central in biotechnology, food industry, and
probiotic production.
Alcoholic Fermentation
• Performedby Yeasts, especially Saccharomyces cerevisiae
• Sugars ethanol + CO₂
→
• Used in wine, beer, bread, toddy
• CO₂ helps in leavening bread
8.
Acetic Acid Fermentation
•Conversion of ethanol acetic acid by Acetobacter
→ aceti
• Aerobic process
• Used in vinegar production
• pH drops to ~3 acts as preservative
→
9.
Alkaline Fermentation
• InvolvesBacillus species, produces ammonia, raises pH
• Common in fermented legumes, fish
• Examples: Natto, kinema, ugba
• Microbe: Bacillus subtilis
Benefits of MicrobialFermentation
• Preservation: Organic acids inhibit spoilage/pathogens
• Improved Digestibility: Breaks down proteins, starches, fibers
• Nutritional Enhancement: B-vitamins, amino acids increase
• Health Effects: Probiotic activity improves gut health
• Flavor & Aroma: Volatile compounds produced
12.
Safety and Qualityin Fermentation
• Fermentation lowers pH and O₂ inhibits
→ Clostridium, Salmonella
• Starter cultures ensure predictable and safe fermentation
• Requires clean equipment and proper time-temperature control
• Traditional methods use back-slopping, but industrial methods prefer
pure cultures.
13.
References:
Prescott, L. M.,Harley, J. P., & Klein, D. A. (2005). Microbiology (6th
ed.). McGraw-Hill.
Pelczar, M. J., Chan, E. C. S., & Krieg, N. R. (2001). Microbiology (5th
ed.). Tata McGraw-Hill.
Frazier, W. C., & Westhoff, D. C. (1995). Food Microbiology (4th ed.).
Tata McGraw-Hill.
Jay, J. M. et al. (2005). Modern Food Microbiology (7th ed.).
Springer.
Adams, M. R., & Moss, M. O. (2008). Food Microbiology (3rd ed.).
RSC.