CMP Degree College
(A Constituent PG College of University of Allahabad)
Topic: Microbiology of fermented
foods
Presented to:
Dr.Avinash singh
Presented by:
Nitesh Kumar Yadav
M.Sc. (Botany) III Semester
DEPARTMENT OF BOTANY
Historical Background of Fermentation
• Fermentation is one of the oldest food preservation techniques, used
for over 8000 years.
• Historical records show beer fermentation in Mesopotamia (6000
BC) and bread-making in Egypt (4000 BC).
• In India, traditional products like dahi, idli, dosa, gundruk have been
fermented for centuries.
• Pasteur (1857) first demonstrated that microorganisms were
responsible for fermentation, not just chemical processes.
• This discovery led to the foundation of industrial microbiology.
Definition and Importance
• Fermentation: Anaerobic breakdown of carbohydrates by
microorganisms into simpler end-products like lactic acid, ethanol,
CO₂, etc.
• Enhances:
◦Shelf-life
◦Nutritional value
◦Digestibility
◦Flavor and texture
◦Safety by inhibiting pathogens
• Fermentation is now central in biotechnology, food industry, and
probiotic production.
Types of Microorganisms Involved
1.Lactic Acid Bacteria (LAB)
◦Lactobacillus, Leuconostoc, Streptococcus, Pediococcus
◦Produce lactic acid lowers pH preservation
→ →
2.Yeasts
◦Saccharomyces cerevisiae, Candida spp.
◦Alcoholic fermentation bread, beer, wine
→
3.Molds
◦Aspergillus, Rhizopus, Penicillium
◦Break complex molecules using enzymes used in soy and cheese
→
fermentation
4. Acetic Acid Bacteria:
- Acetobacter aceti
Classification of Fermentation (Based on Product
Formed)
1.Lactic Acid Fermentation
2.Alcoholic Fermentation
3.Acetic Acid Fermentation
4.Alkaline Fermentation
5.Mixed (Multiple Microbes) Fermentation
Lactic Acid Fermentation
Involves Lactic Acid Bacteria (LAB)
• Anaerobic process sugars converted into
→ lactic acid
• Lowers pH prevents spoilage
→
• Examples: Curd, yogurt, kimchi, idli, sauerkraut
• Bacteria: Lactobacillus bulgaricus, Streptococcus thermophilus
Alcoholic Fermentation
• Performed by Yeasts, especially Saccharomyces cerevisiae
• Sugars ethanol + CO₂
→
• Used in wine, beer, bread, toddy
• CO₂ helps in leavening bread
Acetic Acid Fermentation
• Conversion of ethanol acetic acid by Acetobacter
→ aceti
• Aerobic process
• Used in vinegar production
• pH drops to ~3 acts as preservative
→
Alkaline Fermentation
• Involves Bacillus species, produces ammonia, raises pH
• Common in fermented legumes, fish
• Examples: Natto, kinema, ugba
• Microbe: Bacillus subtilis
Mixed Microbial Fermentation
• Multiple microbial groups act together
• LAB + Yeasts (e.g., Idli, dosa)
• Yeasts + Acetic acid bacteria (e.g., Kombucha)
• Molds + Bacteria (e.g., fermented soy)
• Complex interactions better flavor, texture, safety
→
Benefits of Microbial Fermentation
• Preservation: Organic acids inhibit spoilage/pathogens
• Improved Digestibility: Breaks down proteins, starches, fibers
• Nutritional Enhancement: B-vitamins, amino acids increase
• Health Effects: Probiotic activity improves gut health
• Flavor & Aroma: Volatile compounds produced
Safety and Quality in Fermentation
• Fermentation lowers pH and O₂ inhibits
→ Clostridium, Salmonella
• Starter cultures ensure predictable and safe fermentation
• Requires clean equipment and proper time-temperature control
• Traditional methods use back-slopping, but industrial methods prefer
pure cultures.
References:
Prescott, L. M., Harley, J. P., & Klein, D. A. (2005). Microbiology (6th
ed.). McGraw-Hill.
Pelczar, M. J., Chan, E. C. S., & Krieg, N. R. (2001). Microbiology (5th
ed.). Tata McGraw-Hill.
Frazier, W. C., & Westhoff, D. C. (1995). Food Microbiology (4th ed.).
Tata McGraw-Hill.
Jay, J. M. et al. (2005). Modern Food Microbiology (7th ed.).
Springer.
Adams, M. R., & Moss, M. O. (2008). Food Microbiology (3rd ed.).
RSC.
THANK YOU

Microbiology of fermented foods ppt.pptx

  • 1.
    CMP Degree College (AConstituent PG College of University of Allahabad) Topic: Microbiology of fermented foods Presented to: Dr.Avinash singh Presented by: Nitesh Kumar Yadav M.Sc. (Botany) III Semester DEPARTMENT OF BOTANY
  • 2.
    Historical Background ofFermentation • Fermentation is one of the oldest food preservation techniques, used for over 8000 years. • Historical records show beer fermentation in Mesopotamia (6000 BC) and bread-making in Egypt (4000 BC). • In India, traditional products like dahi, idli, dosa, gundruk have been fermented for centuries. • Pasteur (1857) first demonstrated that microorganisms were responsible for fermentation, not just chemical processes. • This discovery led to the foundation of industrial microbiology.
  • 3.
    Definition and Importance •Fermentation: Anaerobic breakdown of carbohydrates by microorganisms into simpler end-products like lactic acid, ethanol, CO₂, etc. • Enhances: ◦Shelf-life ◦Nutritional value ◦Digestibility ◦Flavor and texture ◦Safety by inhibiting pathogens • Fermentation is now central in biotechnology, food industry, and probiotic production.
  • 4.
    Types of MicroorganismsInvolved 1.Lactic Acid Bacteria (LAB) ◦Lactobacillus, Leuconostoc, Streptococcus, Pediococcus ◦Produce lactic acid lowers pH preservation → → 2.Yeasts ◦Saccharomyces cerevisiae, Candida spp. ◦Alcoholic fermentation bread, beer, wine → 3.Molds ◦Aspergillus, Rhizopus, Penicillium ◦Break complex molecules using enzymes used in soy and cheese → fermentation 4. Acetic Acid Bacteria: - Acetobacter aceti
  • 5.
    Classification of Fermentation(Based on Product Formed) 1.Lactic Acid Fermentation 2.Alcoholic Fermentation 3.Acetic Acid Fermentation 4.Alkaline Fermentation 5.Mixed (Multiple Microbes) Fermentation
  • 6.
    Lactic Acid Fermentation InvolvesLactic Acid Bacteria (LAB) • Anaerobic process sugars converted into → lactic acid • Lowers pH prevents spoilage → • Examples: Curd, yogurt, kimchi, idli, sauerkraut • Bacteria: Lactobacillus bulgaricus, Streptococcus thermophilus
  • 7.
    Alcoholic Fermentation • Performedby Yeasts, especially Saccharomyces cerevisiae • Sugars ethanol + CO₂ → • Used in wine, beer, bread, toddy • CO₂ helps in leavening bread
  • 8.
    Acetic Acid Fermentation •Conversion of ethanol acetic acid by Acetobacter → aceti • Aerobic process • Used in vinegar production • pH drops to ~3 acts as preservative →
  • 9.
    Alkaline Fermentation • InvolvesBacillus species, produces ammonia, raises pH • Common in fermented legumes, fish • Examples: Natto, kinema, ugba • Microbe: Bacillus subtilis
  • 10.
    Mixed Microbial Fermentation •Multiple microbial groups act together • LAB + Yeasts (e.g., Idli, dosa) • Yeasts + Acetic acid bacteria (e.g., Kombucha) • Molds + Bacteria (e.g., fermented soy) • Complex interactions better flavor, texture, safety →
  • 11.
    Benefits of MicrobialFermentation • Preservation: Organic acids inhibit spoilage/pathogens • Improved Digestibility: Breaks down proteins, starches, fibers • Nutritional Enhancement: B-vitamins, amino acids increase • Health Effects: Probiotic activity improves gut health • Flavor & Aroma: Volatile compounds produced
  • 12.
    Safety and Qualityin Fermentation • Fermentation lowers pH and O₂ inhibits → Clostridium, Salmonella • Starter cultures ensure predictable and safe fermentation • Requires clean equipment and proper time-temperature control • Traditional methods use back-slopping, but industrial methods prefer pure cultures.
  • 13.
    References: Prescott, L. M.,Harley, J. P., & Klein, D. A. (2005). Microbiology (6th ed.). McGraw-Hill. Pelczar, M. J., Chan, E. C. S., & Krieg, N. R. (2001). Microbiology (5th ed.). Tata McGraw-Hill. Frazier, W. C., & Westhoff, D. C. (1995). Food Microbiology (4th ed.). Tata McGraw-Hill. Jay, J. M. et al. (2005). Modern Food Microbiology (7th ed.). Springer. Adams, M. R., & Moss, M. O. (2008). Food Microbiology (3rd ed.). RSC.
  • 14.