Staphylococcus aureus

1. Staphylococcus
aureus
Ayesha Ambreen

2. Gram Positive Bacteria ‫ر‬‫ی‬‫ٹ‬‫ک‬‫ی‬‫ی‬‫و‬‫ٹ‬‫ی‬‫از‬‫پ‬ ‫ام‬‫ر‬‫گ‬‫ا‬‫پ‬
‫کس‬‫ک‬‫لوکو‬‫ف‬‫ا‬‫ی‬‫س‬Staphylococcus
•‫کے‬‫و‬‫ج‬ ‫ں‬‫ی‬‫ہ‬ ‫ا‬‫پ‬‫ر‬‫ی‬‫ٹ‬‫ک‬‫ی‬‫ی‬‫والے‬‫ے‬‫ان‬‫ج‬‫ے‬‫ا‬‫پ‬ ‫ر‬‫پ‬ ‫طور‬ ‫ام‬‫ع‬‫ہ‬‫ی‬‫اک‬‫پ‬‫ر‬‫پ‬‫لد‬‫ج‬‫ی‬‫ان‬‫س‬‫ن‬‫ا‬ ،‫ں‬‫ی‬‫م‬‫وا‬‫ہ‬
‫ں‬‫ی‬‫ہ‬‫ے‬‫ون‬‫ہ‬ ‫ود‬‫موج‬ ‫ر‬‫پ‬ ‫لی‬‫ھ‬‫ج‬ ‫ی‬‫درون‬‫اپ‬ ‫کی‬-‫اور‬Commensals‫ں‬‫ی‬‫ہ‬-‫ی‬‫ی‬‫اری‬‫م‬‫ی‬‫ی‬‫ا‬‫د‬
‫ں‬‫ی‬‫ہ‬ ‫ل‬‫پ‬‫ذ‬‫ہ‬‫درج‬‫ی‬‫م‬ ‫ں‬‫ی‬‫م‬‫س‬‫ق‬ ‫ن‬‫ی‬‫ت‬ ‫والی‬ ‫ے‬‫کرن‬-
•‫س‬‫ن‬‫ر‬‫آ‬ ‫لوکوکس‬‫ف‬‫ا‬‫ی‬‫س‬Staphylococcus aureus
.A‫ڈس‬‫پ‬‫ا‬‫م‬‫ڈر‬‫ی‬‫پ‬‫ی‬‫ا‬ ‫لوکوکس‬‫ف‬‫ا‬‫ی‬‫س‬Staphylococcus epidermidis
.B‫ٹ‬‫ی‬‫ا‬‫ف‬‫رو‬‫سی‬ ‫لوکوکس‬‫ف‬‫س‬Staphylococcus saprophyticus

3. Gram Positive Bacteria ‫پازیٹو‬ ‫گرام‬
‫بیکٹیریا‬

4. Morphology ‫صورت‬‫و‬ ‫ل‬‫ک‬‫س‬
•‫ا‬‫ی‬‫ی‬‫ر‬‫ق‬‫ت‬ ‫ے‬‫ھون‬‫ج‬ ‫ے‬‫ھون‬‫ج‬ ‫و‬‫ٹ‬‫ی‬‫از‬‫پ‬ ‫ام‬‫ر‬‫گ‬٠0.5‫سے‬1.0‫طر‬‫ق‬‫ا‬‫ک‬ ‫ان‬‫ر‬‫ک‬‫ی‬‫ی‬‫ا‬‫م‬
((Diameter‫ی‬‫ہ‬‫ے‬‫ت‬‫ہ‬‫ر‬ ‫کر‬ ‫ا‬‫ی‬‫ی‬‫گروپ‬ ‫ا‬‫م‬‫مو‬‫ع‬‫ہ‬‫ی‬‫وڑوں‬‫ج‬‫ھر‬‫پ‬‫ا‬‫پ‬ ‫ں‬
‫ں‬‫ی‬‫ہ‬ ‫ے‬‫ون‬‫ہ‬‫ں‬‫ی‬‫م‬‫صورت‬‫کی‬-

5. Culture Characteristic‫کی‬‫ر‬‫چ‬‫ل‬‫ک‬
‫ت‬‫صی‬‫صو‬‫خ‬
‫ان‬‫کی‬‫دہ‬‫زپ‬‫ے‬‫ت‬‫ہ‬‫ر‬‫کے‬‫ے‬‫ت‬‫ل‬‫ت‬‫ہ‬‫ب‬‫ھوڑی‬‫پ‬‫ھو‬‫پ‬‫ڑی‬oxygen‫کی‬
‫رورت‬‫ض‬‫ی‬‫ون‬‫ہ‬‫ے‬‫ہ‬-Facultative Anaerobe‫گر‬‫م‬
‫ح‬‫ی‬‫ح‬‫ص‬‫ن‬‫ج‬‫ی‬‫س‬‫ک‬‫آ‬‫ل‬‫م‬‫ے‬‫ان‬‫ج‬‫و‬‫ت‬‫ادہ‬‫زپ‬‫ت‬‫ل‬‫ی‬‫ھ‬‫پ‬‫ے‬‫ے‬‫ت‬‫ل‬ ‫ھو‬‫پ‬‫ں‬‫ی‬‫ہ‬-
Aerobic‫ہ‬‫درج‬‫ارت‬‫ر‬‫ح‬35‫سے‬37‫ی‬‫پ‬‫ن‬‫ی‬‫س‬‫ڈ‬‫پ‬‫ر‬‫گ‬‫ان‬‫ک‬‫ے‬‫ے‬‫ت‬‫ل‬
‫ا‬‫ھ‬‫اج‬‫ے‬‫ہ‬-‫ام‬‫ع‬‫طور‬‫ر‬‫پ‬Blood agar ,
MacConkey Agar, Mannitol salt
agar‫اس‬‫کے‬‫ے‬‫ت‬‫ل‬‫ن‬‫ری‬‫ہی‬‫ب‬‫ے‬‫ہ‬-‫لوک‬‫ف‬‫ا‬‫ی‬‫س‬‫وکس‬‫س‬‫ارن‬‫کی‬
‫اں‬‫ی‬‫ی‬‫کولو‬‫ا‬‫م‬‫مو‬‫ع‬‫ک‬‫پ‬‫ا‬‫سے‬‫دو‬‫لی‬‫م‬‫ر‬‫ی‬‫ٹ‬‫م‬‫کی‬‫ی‬‫ون‬‫ہ‬‫ں‬‫ی‬‫ہ‬-‫اور‬‫لے‬‫ی‬‫ی‬
‫گ‬‫پ‬‫ر‬‫کی‬‫ی‬‫ون‬‫ہ‬‫ں‬‫ی‬‫ہ‬-‫اگر‬‫ان‬‫کو‬Blood Agar‫ر‬‫پ‬‫ر‬‫چ‬‫ل‬‫ک‬
‫ک‬‫ا‬‫ی‬
‫ے‬‫ان‬‫ج‬

8. •‫رہ‬‫داپ‬‫ا‬‫س‬‫اف‬‫ف‬‫س‬‫ک‬‫پ‬‫ا‬ ‫اردگرد‬‫ے‬‫ت‬‫ی‬ ‫ا‬‫اں‬‫ی‬‫ی‬‫کولو‬ ‫ض‬‫ع‬‫ت‬ ‫و‬‫ت‬Zone Of
Hemolysis‫ے‬‫ہ‬‫ی‬‫ان‬‫ی‬‫ی‬-‫ک‬ ‫ڈس‬‫اپ‬‫م‬‫ڈر‬‫ی‬‫پ‬‫ی‬‫ا‬ ‫لوکوکس‬‫ف‬‫ا‬‫ی‬‫س‬‫کی‬ ‫گ‬‫پ‬‫ر‬‫د‬‫ی‬‫ف‬‫س‬ ‫ی‬‫الون‬‫ک‬ ‫ی‬
‫ے‬‫ہ‬‫ی‬‫ون‬‫ہ‬-

9. ‫ات‬‫ی‬‫ص‬‫صو‬‫خ‬‫گر‬‫پ‬‫د‬
•Catalase‫کی‬‫ودگی‬‫موج‬‫کی‬‫ہ‬‫وج‬‫سے‬‫ن‬‫ڈروج‬‫ی‬‫ی‬‫ا‬‫ہ‬‫ر‬‫پ‬‫آ‬‫ا‬‫س‬‫ک‬‫ڈ‬‫ی‬‫ی‬‫ک‬‫و‬‫ی‬‫ان‬‫پ‬‫ور‬‫ن‬‫ج‬‫ی‬‫س‬‫ک‬‫آ‬‫ں‬‫ی‬‫م‬‫دل‬‫پ‬‫ی‬‫ی‬‫د‬‫ا‬
‫ے‬‫ہ‬
•‫اس‬‫ں‬‫ی‬‫م‬‫ود‬‫موج‬Coagulase‫سے‬‫ر‬‫ی‬‫ف‬‫ی‬‫ن‬‫وج‬‫ت‬‫کو‬‫ں‬‫ری‬‫ی‬‫ف‬‫ں‬‫ی‬‫م‬‫ل‬‫پ‬‫د‬‫ی‬‫ی‬‫کر‬‫ے‬‫ت‬‫ک‬‫س‬‫ں‬‫ی‬‫ہ‬-

10. ‫سن‬‫ک‬ ‫ا‬‫پ‬‫کی‬ ‫لوکوکس‬‫ی‬‫ف‬‫ا‬‫ی‬‫س‬
Toxins of Staph aureus
‫سن‬‫ال‬ ‫مو‬‫ی‬‫ہ‬ ‫ا‬‫ی‬‫ن‬‫ھ‬‫پ‬ ‫اور‬‫ا‬‫ی‬‫ن‬‫ت‬ ،‫ا‬‫ف‬‫ل‬‫ا‬(Haemolysins)
‫کسن‬ ‫ا‬‫پ‬‫م‬ ‫ڈرو‬‫ی‬‫س‬‫اک‬‫س‬ ‫ک‬‫س‬‫ک‬ ‫ا‬‫پ‬Toxic shock syndrome Toxin
‫کسن‬ ‫ا‬‫پ‬‫و‬‫ٹ‬‫ی‬‫ا‬‫لی‬ ‫و‬‫ف‬‫کس‬‫پ‬‫ا‬Exofoliative Toxin
‫ی‬‫ا‬‫س‬‫کو‬‫ی‬‫ل‬‫ڈن‬Leukicidin
‫اکسن‬‫پ‬‫رو‬‫ی‬‫ٹ‬‫ی‬‫ت‬ ‫ا‬Enterotoxin

11. ‫نقصانات‬
,‫اں‬‫ی‬‫س‬‫ن‬‫ھ‬‫پ‬‫ھوڑے‬‫پ‬Boils, abscess
‫ن‬‫کس‬‫ی‬‫ف‬‫ت‬‫ا‬ ‫کی‬ ‫م‬‫ج‬‫ز‬Wound infection
‫ن‬‫کس‬‫ی‬‫ف‬‫ت‬‫ا‬ ‫کی‬ ‫ال‬‫ی‬‫ن‬‫س‬‫ہ‬Hospital infection
‫ن‬‫کس‬‫ی‬‫ف‬‫ت‬‫ا‬‫ا‬‫ک‬ ‫ھوں‬‫ک‬‫پ‬‫آ‬Conjunctivitis
‫ا‬‫ی‬‫ی‬‫مو‬‫ی‬‫ی‬Pneumonia
‫گ‬‫ی‬‫ی‬‫ز‬‫وا‬‫ت‬‫وڈ‬‫ف‬Food Poisoning

13. Biochemistry
•Catalase +ve
•Coagulase +ve
•DNase +ve
•Phosphate +ve
•Mennitol +ve
•Fermentation +ve
•VP +ve

14. Antibiotic Sensitivity
‫ت‬‫ی‬‫س‬‫ا‬‫س‬‫ح‬‫سے‬‫ک‬‫ی‬‫ی‬‫و‬‫ت‬‫ی‬‫پ‬‫ی‬‫ا‬
‫ھی‬‫ی‬‫ن‬‫م‬
،‫ن‬‫ی‬‫ل‬‫ک‬‫ت‬ ‫ا‬‫س‬‫ا‬‫ر‬‫ی‬‫ٹ‬‫ت‬،‫ن‬‫ی‬‫ی‬‫م‬‫گ‬ ‫آ‬،‫لن‬‫س‬‫ن‬‫ی‬‫ی‬‫ے‬‫ت‬‫ل‬ ‫کے‬ ‫اس‬‫ا‬‫م‬‫مو‬‫ع‬‫ل‬ ‫ک‬‫س‬‫ڈ‬‫کی‬ ‫ن‬‫وری‬‫ٹ‬‫س‬‫لو‬‫ف‬‫س‬ ‫اور‬‫ن‬‫ی‬‫ل‬‫س‬‫گی‬
‫ے‬‫ہ‬‫ی‬‫ان‬‫ج‬

15. Staph saprophyticus ‫کس‬‫ی‬‫ن‬‫ی‬‫ف‬‫رو‬‫سی‬‫ف‬‫ی‬‫ن‬‫س‬
‫ن‬‫کس‬‫ی‬‫ف‬‫ت‬‫ا‬ ‫کی‬ ‫اپ‬‫س‬‫ن‬‫ت‬ ‫ہ‬‫ی‬UTIUrinary Tract Infection))‫ے‬‫ہ‬‫ا‬‫پ‬‫ر‬‫ک‬-
‫ے‬‫ہ‬ ‫سی‬‫ن‬‫ج‬‫س‬‫اورن‬‫ف‬‫ی‬‫س‬‫ت‬‫خ‬‫ا‬‫س‬ ‫کی‬ ‫اس‬-

16. Staph epidermidis ‫ڈس‬‫ی‬‫ی‬‫ا‬‫م‬‫ڈر‬‫ی‬‫پ‬‫ی‬‫ا‬‫ف‬‫ی‬‫ن‬‫س‬
•‫ے‬‫ہ‬ ‫لورا‬‫ف‬‫ل‬‫م‬‫ار‬‫پ‬‫ا‬‫ک‬ ‫لد‬‫ج‬‫ہ‬‫ی‬-‫ج‬‫و‬‫ہ‬‫ل‬‫م‬‫ا‬‫س‬ ‫ں‬‫ی‬‫م‬‫ون‬‫ج‬ ‫ہ‬‫ی‬‫اگر‬ ‫اور‬‫و‬‫ت‬‫ے‬‫ان‬
Bacteremia‫ے‬‫ہ‬‫ا‬‫پ‬‫ر‬‫ک‬-
•‫ن‬‫کس‬‫ی‬‫ف‬‫ت‬‫ا‬‫ا‬‫ک‬ ‫لی‬‫ھ‬‫ج‬ ‫کی‬ ‫دل‬‫ہ‬‫ی‬Endocarditis‫ے‬‫ہ‬‫ا‬‫پ‬‫ر‬‫ک‬–‫گ‬‫پ‬‫ر‬‫د‬‫ی‬‫ف‬‫س‬‫ہ‬‫ی‬‫کی‬
‫ں‬‫ی‬‫ہ‬ ‫ے‬‫ن‬‫ا‬‫ی‬‫ی‬‫اں‬‫ی‬‫ی‬‫کولو‬-

17. Staphylococcus Medical Importance
Species Hemolysis Coagulase
Production
Catalase Typical
Disease
Staph. aureus Beta + + Abscess, food
poisoning,
toxic shock
syndrome
Staph.
epidermidis
None _ + Infection of
prosthetic heart
valves and
hips; common
member of skin
flora
Staph.
saprophyticus
None _ + Urinary tract
infection