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Microscope parts and functions


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Microscope parts and functions

  1. 1. Microscope Parts and FunctionsPresented by: Joevani S. Peñol
  2. 2. Microscope• An optical instrument that uses a lens or acombination of lenses to produce magnifiedimages of small objects, especially of objectstoo small to be seen by the unaided eye.
  3. 3. Microscope Parts and Functions• Arm- Supports the tube and connects it to thebase• Base- The bottom of the microscope, used forsupport• Eyepiece- Where you look to see the image ofyour specimen.
  4. 4. ArmEyepieceBase
  5. 5. Microscope Parts and Functions• Body tube- Connects the eyepiece to theobjective lenses.• Revolving Nosepiece- This is the part thatholds two or more objective lenses and can berotated to easily change power.• Stage- The flat platform where you place yourslides.
  6. 6. Body tubeStageRevolvingNosepiece
  7. 7. Microscope Parts and Functions• Fine Adjustment Knob-small, round knob onthe side of the microscope used to fine-tunethe focus of your specimen• Coarse Adjustment Knob--large, round knobon the side of the microscope used forfocusing the specimen.• Stage Clips-hold the slide in place
  8. 8. CoarseAdjustment KnobFine AdjustmentKnobStage Clips
  9. 9. Microscope Parts and Functions• Iris Diaphragm- controls the light goingthorough the aperture.• Mirror/Light source-used to reflect light to thespecimen/source of light.• Objective lenses-may have (scanner, Low, highOil immersion objectives) and is used use toincrease the magnification of the specimen.
  10. 10. Iris DiaphragmObjectiveLensesLight Source
  11. 11. Microscope Parts and Functions• Aperture is the hole in the stage throughwhich the base (transmitted) light reaches thestage.• Condenser is used to collect and focus thelight from the illuminator on to the specimen.It is located under the stage often inconjunction with an iris diaphragm.
  12. 12. Proper Way of Focusing theMicroscope• Always observe the specimen or object usingthe LOWEST POWER object first.• Focus using the COARSE ADJUSTMENT KNOBto bring the object into focus. Bring the objectinto sharp focus by using the fine adjustmentknob.
  13. 13. Proper Way of Focusing theMicroscope• Focus, and then move to a higher powerobjective, if needed.• Use only the FINE ADJUSTMENT KNOB whenusing the HIGHEST (longest) POWEROBJECTIVE.
  14. 14. Proper Way of Focusing theMicroscope• Keep both eyes open to reduce eyestrain.Keep eye slightly above the eyepiece toreduce eyelash interference.• To find out the total magnification of theobject, multiply the power of the eyepiecelens (10X) by the power of the objective.
  15. 15. Handling the Microscope• Always use two hands to move themicroscope. Place one hand around the arm,lift the scope, and then put your other handunder the base of the scope for support.• Be gentle.
  16. 16. Storing the Microscope• Dust is an enemy to microscope lenses; alwayskeep the microscope covered when not in use.
  17. 17. References•••