Development and validation of rphplc method for nsai ds in combined pharmaceu...IJSIT Editor
The present research work includes the development and validation of High performance liquid
chromatography method for simultaneous estimation of Etirocoxib (ETX )and Paracetamol (PCM) which are
very much of useful in multiple therapies rather than the use of single drug formulation, because of multiple
actions, fever side effect ,and quicker relief. We aimed to design a quick and simple method, suitable for the
determination of identity, strength. The UV spectroscopic method were developed on standard ETX and PCM
using methanol as standard solution and also, the standard stock solution of both ETX and PCM were diluted
with 2%hydrochloric acid to obtain concentration 2.0µg/ml and 16.6µg/ml.The spectrum was recorded in the
range of 400-200nm.The RP- HPLC method were developed on standard ETX and PCM which performed on C-
18 column using mobile phase composed by acetonitrile :water 50:50(v/v) at a flow rate of 1 ml/min, with
injection volume 20µl and Ph 6.8 can be adjusted using orthophosphoric acid.
We report the 11-year follow-up of a premenopausal woman with osteogenesisimperfecta (OI) who
was treated with alendronate. A 41-year-old Japanese premenopausal woman with OI type I who had
frequently experienced painful fragility fractures consulted our clinic because of chronic back pain associated
with spinal osteoporosis. She had undergone heart surgery (aortic valve replacement) because of aortic
regurgitation 5 years before her first consultation with our clinic. After surgery, she began taking warfarin (3
mg/day), and this treatment was continued during our follow-up period. She was treated with alendronate (5
mg/day or 35 mg/week) for 11 years. The patient’s urinary cross-linked N-terminal telopeptides of type I
collagen and serum alkaline phosphatase levels decreased, while the bone mineral density of her lumbar
spine (L2–L4) increased, as measured using dual energy X-ray absorptiometry. The serum calcium and
phosphorus levels stayed within the normal ranges. Three non-vertebral fractures occurred at the hip, ankle,
and ring finger during the 11-year treatment period, but no adverse effects were observed. Thus, the present
case report showed the long-term outcome and safety of alendronate treatment in a premenopausal woman
with OI type I.
SYNTHESIS AND CHARACTERISATION OF µ-OXY-BIS [TRIARYLANTIMONY (V)] DICARBOXYLA...IJSIT Editor
Several hitherto to unknown µ-oxybis[triarylantimony(V)]dicarboxylates & µ-oxy-
bis[triarylantimony]halo-carboxylates of the general formula R3Sb(L)-O-Sb(L)R3 & R3Sb(Cl)-O-Sb(L)R3 respectively
have been synthesised by the metathetical reactions of µ-oxybis(triarylantimony)dichloride reactions and silver salts
of corresponding carboxylic acids in 1:2 & 1:1 ratio [where L= 2-pyrazine carboxylic acid, p-methoxy medallic acid,
salicylic acid, benzillic acid, )]. The newly synthesised antimony derivatives have been characterised on the basis of
melting point, elemental analysis, IR
IH &
13C NMR spectra. The molecular weight & conductivity data indicate the
monomeric & non electrolytic behaviour in solution, these have been found to exhibit moderate to significant
antimicrobial activity.
Development and validation of rphplc method for nsai ds in combined pharmaceu...IJSIT Editor
The present research work includes the development and validation of High performance liquid
chromatography method for simultaneous estimation of Etirocoxib (ETX )and Paracetamol (PCM) which are
very much of useful in multiple therapies rather than the use of single drug formulation, because of multiple
actions, fever side effect ,and quicker relief. We aimed to design a quick and simple method, suitable for the
determination of identity, strength. The UV spectroscopic method were developed on standard ETX and PCM
using methanol as standard solution and also, the standard stock solution of both ETX and PCM were diluted
with 2%hydrochloric acid to obtain concentration 2.0µg/ml and 16.6µg/ml.The spectrum was recorded in the
range of 400-200nm.The RP- HPLC method were developed on standard ETX and PCM which performed on C-
18 column using mobile phase composed by acetonitrile :water 50:50(v/v) at a flow rate of 1 ml/min, with
injection volume 20µl and Ph 6.8 can be adjusted using orthophosphoric acid.
We report the 11-year follow-up of a premenopausal woman with osteogenesisimperfecta (OI) who
was treated with alendronate. A 41-year-old Japanese premenopausal woman with OI type I who had
frequently experienced painful fragility fractures consulted our clinic because of chronic back pain associated
with spinal osteoporosis. She had undergone heart surgery (aortic valve replacement) because of aortic
regurgitation 5 years before her first consultation with our clinic. After surgery, she began taking warfarin (3
mg/day), and this treatment was continued during our follow-up period. She was treated with alendronate (5
mg/day or 35 mg/week) for 11 years. The patient’s urinary cross-linked N-terminal telopeptides of type I
collagen and serum alkaline phosphatase levels decreased, while the bone mineral density of her lumbar
spine (L2–L4) increased, as measured using dual energy X-ray absorptiometry. The serum calcium and
phosphorus levels stayed within the normal ranges. Three non-vertebral fractures occurred at the hip, ankle,
and ring finger during the 11-year treatment period, but no adverse effects were observed. Thus, the present
case report showed the long-term outcome and safety of alendronate treatment in a premenopausal woman
with OI type I.
SYNTHESIS AND CHARACTERISATION OF µ-OXY-BIS [TRIARYLANTIMONY (V)] DICARBOXYLA...IJSIT Editor
Several hitherto to unknown µ-oxybis[triarylantimony(V)]dicarboxylates & µ-oxy-
bis[triarylantimony]halo-carboxylates of the general formula R3Sb(L)-O-Sb(L)R3 & R3Sb(Cl)-O-Sb(L)R3 respectively
have been synthesised by the metathetical reactions of µ-oxybis(triarylantimony)dichloride reactions and silver salts
of corresponding carboxylic acids in 1:2 & 1:1 ratio [where L= 2-pyrazine carboxylic acid, p-methoxy medallic acid,
salicylic acid, benzillic acid, )]. The newly synthesised antimony derivatives have been characterised on the basis of
melting point, elemental analysis, IR
IH &
13C NMR spectra. The molecular weight & conductivity data indicate the
monomeric & non electrolytic behaviour in solution, these have been found to exhibit moderate to significant
antimicrobial activity.
کیا فرماتے ہیں علمائے دین اس مسئلے میں کہ سرورکائنات فخر موجودات رسول خدا محمدمصطفی صلی اللہ تعالٰی علیہ وآلہٖ وسلم کے ماں باپ آدم علٰی نبینا وعلیہ السلام تک مومن تھے یا نہیں؟بینوا توجروا۔(عہ۱) (بیان کرو اجر پاؤگے ۔ت)
کیا فرماتے ہیں علمائے دین اس مسئلے میں کہ سرورکائنات فخر موجودات رسول خدا محمدمصطفی صلی اللہ تعالٰی علیہ وآلہٖ وسلم کے ماں باپ آدم علٰی نبینا وعلیہ السلام تک مومن تھے یا نہیں؟بینوا توجروا۔(عہ۱) (بیان کرو اجر پاؤگے ۔ت)