Human derived Growth factor

1. Human Derived Growth Factor
A Game Changer in Trichology

2. Introduction
 Alopecia is a common and distressing medical condition affecting a
majority of men and women worldwide by middle age.
 Significant emotional and physical burden of alopecia, it is essential
for clinicians to regard hair loss as more than a cosmetic issue.
 Alopecia often can be a sign of various systemic conditions, such as
autoimmune disease, anemia, nutritional deficiency, and chronic
infection.
Med Clin North Am. 2021 Jul;105(4):599-610.

3. Types of alopecia
Alopecia
•Androgenetic Alopecia
•Telogen Effluvium
•Alopecia Areata
Med Clin North Am. 2021 Jul;105(4):599-610.

4. Androgenetic alopecia
 An extremely common disorder affecting both sexes, although its incidence is generally greater in
men than in women
 A genetically predetermined disorder caused by excessive response to androgens
 Occurs any time after puberty and characterized by progressive loss of terminal hair usually in a
pattern distribution.
 Male (MPHL): hair loss is mostly observed in vertex and frontotemporal regions
 Female (FPHL): frontal hairline is typically spared with diffuse apical hair loss noted as a wider
anterior part of the hair
Ho CH, et al. Androgenetic Alopecia. [Updated 2021 May 5]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan-Accessed July 6, 2021. 11:50 PM. Available
from: https://www.ncbi.nlm.nih.gov/books/NBK430924/
Mysore V, et al. Expert consensus on the management of Androgenetic Alopecia in India. Int J Trichology. 2019;11(3):101-106.

5. Telogen Effluvium Alopecia Areata
• Sudden hair loss occurs diffusely
from the scalp approximately 2-3
months after the triggering event
• Thinning of hair all over the scalp
• Chronic telogen effluvium (CTE) is a
diffuse hair loss of scalp that persist
for more than six months
• Alopecia areata is a common
autoimmune disorder that often
results in unpredictable hair loss
• Hair falls out in small patches around
the size of a quarter.
• Alopecia can affect anyone,
regardless of age, gender, or race

6. Clinical Features and Grading
Mysore V, et al. Expert consensus on the management of Androgenetic Alopecia in India. Int J
Trichology. 2019;11(3):101-106.
Cm, centimetre; FT, frontotemporal
Modified Norwood-Hamilton classification of male pattern androgenetic alopecia
I II IIa IIIa III
IIIvertex IV IVa V Va
VI VII
Int J Trichology. 2019 May-Jun; 11(3): 101–106.

7. Ludwig’s scale for female androgenetic alopecia
Clinical Features and Grading
Mysore V, et al. Expert consensus on the management of Androgenetic Alopecia in India. Int J
Trichology. 2019;11(3):101-106.
Stage 1 Severity of female hair loss
Stage 2
Thinning of hair is seen mainly over the anterior part
of the crown with minimal widening of
the parting width
Thinning of the crown becomes more evident
because of an increase in the number of thin and
Short hairs
The crown becomes almost total bald. There is
significant widening of the parting width, but
The frontal hairline is still maintained
Stage 3

8. Epidemiology
 Androgenetic alopecia
In Males - 50% affected by 50 years old and up to 80% affected by 70 years old.
In females - the disorder is quite common, with an increase in incidence after menopause.
 Telogen Effluvium
-The association of telogen effluvium with age is unclear; however, elderly women are known to be more
susceptible.
1. Asghar F, Shamim N, Farooque U, Sheikh H, Aqeel R. Telogen Effluvium: A Review of the Literature. Cureus. 2020 May 27;12(5):e8320.
2. Ho CH, et al. Androgenetic Alopecia. [Updated 2021 May 5]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2021 Jan-Accessed July 6,
2021. 11:50 PM

9. Treatment
Medical therapy
Dietary
supplements
Minimal invasive
procedures

10. Management
1. Lee SW, et al. A Systematic Review of Topical Finasteride in the Treatment of Androgenetic Alopecia in Men and Women. J Drugs Dermatol. 2018;17(4):457-463.
2. Hosking AM, et al. Complementary and alternative treatments for alopecia: a comprehensive review. Skin Appendage Disord. 2019;5(2):72-89.
3. Fabrizio L, et al. Evaluation of A Pool of Biomimetic Peptides on Human Hair Follicles: A Preclinical Study. Archives of Dermatology and Skin Care. 2019;2(1):11-17.
PRP, platelet-rich plasma.
Treatment
Minoxidil Finasteride Growth factors Peptides PRP
Approved
therapy
Other therapy

11. Growth factors are natural proteins
capable of stimulating cellular
growth and proliferation
Fibroblasts
Keratinocytes
Naturally produce
growth factors
https://www.dermnetnz.org/topics/role-of-growth-factors-in-skin
What are Growth Factors?

12. Action of growth factors
Growth factors bind to specific receptors on the plasma membrane to trigger
cellular growth and proliferation.
https://www.dermnetnz.org/topics/role-of-growth-factors-in-skin

13. Action of growth factors
These growth factors bind to specific
receptors on cell surface of target cells
↓
Regulate the biological activities responsible
for repairing cellular and genetic damage,
promote skin tissue repair and regeneration
↓
They promote the formation of collagen and
elastic fibres which give the skin its softness
and suppleness
https://www.dermnetnz.org/topics/role-of-growth-factors-in-skin

14. Role of growth factor in Skin ageing and wound healing
Rahul Mehta and Richard Fitzpatrick. Endogenous growth factors as cosmeceuticals.Dermatologic Therapy, Vol. 20, 2007, 350–359.

15. Types of growth factors
HB – EGF
Keratinocyte and
fibroblast mitogen
FGFs
1,2 & 4
Angiogenic and
fibroblast mitogen
PDGF
Chemotactic for
macrophages,
fibroblasts & mitogen
IGF-1
Endothelial cell
& fibroblast
mitogen
TGF-β
1,2 & 3
Keratinocyte
migration
IL - 1α & β
Early activators of
growth factor
expression in
macrophages,
keratinocytes &
fibroblasts
Caroline Aldag, Diana Nogueira Teixeira,Phillip S Leventha. Skin rejuvenation using cosmetic products containing growth factors, cytokines, and matrikines: a
review of the literature.Volume 2016:9 Pages 411—419

16. Plant and animal based growth factors
Derived from animals Derived from plants and plant culture

17. Recombinant human growth factor
It is safe because it is 100% homologous to human proteins—no expectation of any allergic
reaction from topical application
Source
Growth Factor products are engineered with advanced stem cell technology,
the source plays an important role.
A synthesized human gene joined to bacteria is used to manufacture growth
factors on a commercial scale
Data on file

18. GROFACTOR
Hair SYSTEM
GF Hair care Complex
Nourishing blend of growth factors,
moisturizers, vitamins, botanicals, and
peptides designed to restore the normal
growth cycle of hair follicles with growth
factors that achieve maximum penetration
and benefits to the scalp
Data on file

19. Origin of Grofactor
The Benev Advantage
Benev California USA, uses only premium sources for our growth
factor ingredients, and scientifically advanced methods of production
and filtration ensure highly stable, pure, concentrated product
 FDA-registered manufacturer, which requires quality production
methods and stringent quality control
 Controlled lab and trained technicians
 Formula focus is on the most effective growth factors for dermal
application
Data on file
BENEV growth factor is highly concentrated, topically applied,
purified growth factor formula that is designed for use with clinical
modalities that enhance penetration
Data on file

20. Combinations with growth factors
Hair loss control and Hair growth
EGF + FGF
Restores normal,
healthy hair growth
cycle
Biotinoyl tripeptide:
Scalp & Hair nourishment
Apigenin:
Stimulates hair growth
Acts as an anti-oxidant and anti-
inflammatory
Vit B6
Improves circulation of nutrients
1. BioMed Research International Volume 2015, Article ID 730139, 9 pages
2. https://alitura.com/blogs/blog/benefits-of-apigenin-the-beautifying-flavonoid
3. https://www.tabletwise.net/medicine/biotinoyl-tripeptide-1
4. https://nutrafol.com/blog/vitamins-hair-growth-role-vitamin-b6-promoting-healthy-
hair/

21. Indication
Best candidates
Men:
• Androgenetic alopecia
• Stress-related hair loss
• Drug-induced hair loss
Women:
• Female pattern baldness (FPB).
• Pre/post-menopausal hair loss
• Stress-related hair loss,
Data on file

22. How to use growth factor of hair system
 Dermaroller 
 Derma Pen 
Micro-needling
Data on file

23. Procedure

24. Before microneedling
 Advise patient to come in with just washed hair, without using products such as hairspray,
styling gel, etc
 Section the head (into 4 parts)
- Crown
- Frontal Right side (back of ear forward)
- Frontal Left side
- Back
 Use 2ml of gro factor apply to the scalp for each session - Apply o.5 ml topically to the each
part mentioned above (2ml total)
Data on file

25. During microneedling
 After application of gro factor to each part, micro-needling is done directly after each parting.
 It should be done row by row in each part
 Rolling is done 5 times each in the horizontal, vertical, and oblique directions
 The treatment endpoint is identified as uniform pin-point bleeding which is easily controllable
Data on file

26. After microneedling
 When completed, gently comb any tangles from hair.
 Patient should refrain from shampooing hair for 24 hours
Data on file

27. Protocol
 One session once a week - followed by 2-3 weeks gap
 Around 4-6 sessions are needed
Data on file

28. Clinical evidence

29. Grofactor Hair care complex Study I
Objective:
To determine if the application of growth factors (GF Hair Care Complex) would help stop
hair loss and effect hair re-growth
Methods:
76 subjects aged 21—66, approximately half female and half male,
Subjects underwent approximately ten twice weekly GF Hair Care Complex with
SkinStamp VMT procedures (number of treatments varied based on individual patient
assessments after first five treatments), with a ten-day break after the first five
Treatments
Data on file

30. Grofactor Hair care complex Study I
Data on file
Hair Care Complex combined with micro needling treatment results
in 81% improvement in appearance of hair growth

31. Grofactor Hair care complex Study II
Objectives and methods:
Males aged 25—65 years of age with mild to moderate AGA was
conducted to test the effectiveness of BENEV® GF Hair Care Complex
with SkinStamp VMT in preventing hair loss and producing re-growth.
Data on file

32. Results
Data on file
Over 90% of study subjects showed improvement.

33. Results after 5 months
Data on file

34. Results after 5 months
Data on file

35. Results:
HAIR CARE COMPLEX 56 YEAR OLD MALE AT WEEK 12
Norwood scale between 3 and 6
Data on file

36. Advantages over Mesotherapy
 In mesotherapy only vitamin and peptides are present, no growth factor
 Grofactor is 100% homologous to human protein therefore less chances of allergy
 Grofactor is standardized formulation
Data on file

37. Advantages over PRP
 Grofactor is a concentrated enhanced growth factor solution. Apart from Growth Factor it also
contains other Vitamins/ Peptides (In PRP only growth factor is present )
 Does not require any blood extraction. No Pain or Pricks (Injections)
 Standardized formulation
 Less time consuming
 No Centrifuge
Data on file

38. Advantages over laser
 Lasers only triggers the cells to produce growth Factors while Grofactor (Growth Factors with
Vitamins/ Peptides) will be an add-on to the laser therapy.
 Grofactor is a cost effective choice while laser is expensive
Data on file

39. CurrentpracticewithGrowthFactors
Monotherapy
Mix it with PRP :
Intermittent
sessions of PRP
With dermaroller or
dermapen
3 sessions of PRP and 3 of
Gro factor alternately
Data on file

40. Take home message
 Growth factors are natural proteins capable of stimulating cellular growth and proliferation
 Gro factor is of human origine. 100% homologous to human protein, No allergic reaction
 Manufactured in US FDA approved facility
 Along with Growth Factor it also contains other Vitamins and Peptides
 Gro factor hair system is used with derma roller or dermapen
 Combining growth factors with PRP with alternate sessions will provide a synergistic effect.

41. Thank You