Flow Imaging Microscopy Equipment

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Akoya announced the launch of PhenoCode™ Signature Panels for high-throughput spatial biomarker discovery and validation on the PhenoImager® platforms, providing the flexibility to keep pace with the dynamic combination therapy landscape. Additionally, Akoya has brought new datasets of spatial imaging of complementary RNA and protein biomarkers on the PhenoCycler®-Fusion system, which reveal insights into tumor biology and immunotherapyresponse..https://www.gaiascience.com.sg/branddetails/akoya-biosciences

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Thermo Scientific Phenom ParticleX Desktop SEM (Scanning Electron Microscope) is a multi-purpose desktop SEM designed for multiple applications at the microscale. The Thermo Scientific Phenom ParticleX is a versatile desktop SEM solution for high quality analysis in-house. It gives you the ability to carry out speedy analysis, verification and classification of materials, supporting, your production with fast, accurate and trusted data..https://www.gaiascience.com.sg/productdetails/phenom-particlex-desktop-sem

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Erlab Captair Bio 391 Smart Mobile Ductless Filtering PCR Workstation features a high efficiency filtration system that provides a particulate free atmosphere around the manipulation. A high energy UV light is used to decontaminate the worktop from biological cross-contamination between operations..https://www.gaiascience.com.sg/productdetails/captair-bio-391-smart--mobile-ductless-filtering-pcr-workstation

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Percival Scientific makes various scientific equipment including the best biological incubators, research chambers, environment control systems etc. Phenospex is a biotech company, which develops and provides hard- and software for automated plant screening and plant phenotyping. JIUPO's main products are environmental controlled rooms, movable plant growth chamber, plant lighting solutions which range from standard style to highly configured style and customized solutions..https://www.gaiascience.com.sg/subcategories/plant-biology

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We explore different scenarios to explain the chemical difference found in the remarkable giant-giant binary system HD138202+CD−3012303. For the first time, we suggest how to distinguish these scenarios by taking advantage of the extensive convective envelopes of giant stars. Methods. We carried out a high-precision determination of stellar parameters and abundances by applying a full line-by-line differential analysis on GHOST high-resolution spectra. We used the FUNDPAR program with ATLAS12 model atmospheres and specific opacities calculated for an arbitrary composition through a doubly iterated method. Physical parameters were estimated with the isochrones package and evolutionary tracks were calculated via MIST models. Results. We found a significant chemical difference between the two stars (∆[Fe/H]∼0.08dex), which is largely unexpected considering the insensitivity of giant stars to planetary ingestion and diffusion effects. We tested the possibility of engulfment events by using several different combinations of stellar mass, ingested mass, metallicity of the engulfed object and different convective envelopes. However, the planetary ingestion scenario does not seem to explain the observed differences. For the first time, we distinguished the source of chemical differences using a giant-giant binary system. By ruling out other possible scenarios such as planet formation and evolutionary effects between the two stars, we suggest that primordial inhomogeneities might explain the observed differences. This remarkable result implies that the metallicity differences that were observed in at least some main-sequence binary systems might be related to primordial inhomogeneities rather than engulfment events. We also discuss the important implications of finding primordial inhomogeneities, which affect chemical tagging and other fields such as planet formation. We strongly encourage the use of giantgiant pairs. They are a relevant complement to main-sequence pairs for determining the origin of the observed chemical differences in multiple systems.

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A botany file is similar to a chemistry practical file but specifically focuses on experiments and observations related to plants. It typically includes detailed descriptions of plant-related experiments, such as germination studies, growth experiments, tissue culture techniques, plant anatomy investigations, and ecological surveys. The file may contain records of plant specimens collected, their classification, characteristics, and ecological significance. Additionally, it may include diagrams, photographs, drawings, tables, and data analysis related to plant research. Like a chemistry practical file, a botany file serves as a documentation of practical work conducted in the field of botany and is often used for assessment and educational purposes.

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HotJupiters are amongthebest-studied exoplanets, but it is still poorly understood how their chemical composition and cloud properties vary with longitude. Theoretical models predict that clouds may condense on the nightside and that molecular abundances can be driven out of equilibrium by zonal winds. Here we report a phase-resolved emission spectrum of the hot Jupiter WASP-43b measured from 5–12µ 5–12µ 5–12µm with JWST’s Mid-Infrared Instrument (MIRI). 1524 ±35 1524 ±35 and 863±23 The spectra reveal a large day–night temperature contrast (with average brightness temperatures of 1524 ± 35 863 ±23 863 ±23Kelvin, respectively) and evidence for water absorption at all orbital phases. Comparisons with three-dimensional atmospheric models show that both the phase curve shape and emission spectra strongly suggest the presence of nightside clouds which become optically thick to thermal emission at pressures greater than ∼100mbar. The dayside is consistent with a cloudless atmosphere above the mid-infrared photosphere. Con3trary to expectations from equilibrium chemistry but consistent with disequilibrium kinetics models, methane is not detected on the nightside (2σ upper limit of 1–6 parts per million, depending on model assumptions).

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Context. WASP-76 b has been a recurrent subject of study since the detection of a signature in high-resolution transit spectroscopy data indicating an asymmetry between the two limbs of the planet. The existence of this asymmetric signature has been confirmed by multiple studies, but its physical origin is still under debate. In addition, it contrasts with the absence of asymmetry reported in the infrared (IR) phase curve. Aims. We provide a more comprehensive dataset of WASP-76 b with the goal of drawing a complete view of the physical processes at work in this atmosphere. In particular, we attempt to reconcile visible high-resolution transit spectroscopy data and IR broadband phase curves. Methods. We gathered 3 phase curves, 20 occultations, and 6 transits for WASP-76 b in the visible with the CHEOPS space telescope. We also report the analysis of three unpublished sectors observed by the TESS space telescope (also in the visible), which represents 34 phase curves. Results. WASP-76 b displays an occultation of 260±11 and 152±10 ppm in TESS and CHEOPS bandpasses respectively. Depending on the composition assumed for the atmosphere and the data reduction used for the IR data, we derived geometric albedo estimates that range from 0.05 ± 0.023 to 0.146 ± 0.013 and from <0.13 to 0.189 ± 0.017 in the CHEOPS and TESS bandpasses, respectively. As expected from the IR phase curves, a low-order model of the phase curves does not yield any detectable asymmetry in the visible either. However, an empirical model allowing for sharper phase curve variations offers a hint of a flux excess before the occultation, with an amplitude of ∼40 ppm, an orbital offset of ∼−30◦ , and a width of ∼20◦ . We also constrained the orbital eccentricity of WASP-76 b to a value lower than 0.0067, with a 99.7% confidence level. This result contradicts earlier proposed scenarios aimed at explaining the asymmetry observed in high-resolution transit spectroscopy. Conclusions. In light of these findings, we hypothesise that WASP-76 b could have night-side clouds that extend predominantly towards its eastern limb. At this limb, the clouds would be associated with spherical droplets or spherically shaped aerosols of an unknown species, which would be responsible for a glory effect in the visible phase curves.

Asymmetry in the atmosphere of the ultra-hot Jupiter WASP-76 b

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Understanding circumstellar disks is of prime importance in astrophysics, however, their birth process remains poorly constrained due to observational and numerical challenges. Recent numerical works have shown that the small-scale physics, often wrapped into a sub-grid model, play a crucial role in disk formation and evolution. This calls for a combined approach in which both the protostar and circumstellar disk are studied in concert. Aims. We aim to elucidate the small scale physics and constrain sub-grid parameters commonly chosen in the literature by resolving the star-disk interaction. Methods. We carry out a set of very high resolution 3D radiative-hydrodynamics simulations that self-consistently describe the collapse of a turbulent dense molecular cloud core to stellar densities. We study the birth of the protostar, the circumstellar disk, and its early evolution (< 6 yr after protostellar formation). Results. Following the second gravitational collapse, the nascent protostar quickly reaches breakup velocity and sheds its surface material, thus forming a hot (∼ 103 K), dense, and highly flared circumstellar disk. The protostar is embedded within the disk, such that material can flow without crossing any shock fronts. The circumstellar disk mass quickly exceeds that of the protostar, and its kinematics are dominated by self-gravity. Accretion onto the disk is highly anisotropic, and accretion onto the protostar mainly occurs through material that slides on the disk surface. The polar mass flux is negligible in comparison. The radiative behavior also displays a strong anisotropy, as the polar accretion shock is shown to be supercritical whereas its equatorial counterpart is subcritical. We also f ind a remarkable convergence of our results with respect to initial conditions. Conclusions. These results reveal the structure and kinematics in the smallest spatial scales relevant to protostellar and circumstellar disk evolution. They can be used to describe accretion onto regions commonly described by sub-grid models in simulations studying larger scale physics.

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The primary distinction between a test cross and a backcross is that a test cross is used to determine the genotype of a phenotypically dominant individual, whereas a backcross is used to recover a dominant genotype from a parent who has an extreme genotype. The hybrids that are backcrossed are known as 'BC' hybrids. For example, a BC1 hybrid is an F1 hybrid that was crossed with one of its parents or a genetically similar species. The BC2 hybrid is defined as a BC1 hybrid that has been crossed with the same parent or genetically similar species. Other instances include backcrossing in animals. A system of breeding in which repeated backcrosses are made to transfer a specific character to a well-adapted variety for which the variety is deficient is referred to as backcross breeding This approach is infrequently utilized in vegetatively propagated crops such as sugarcane and potatoes, and only with slight alterations. A test cross is a genetic technique for determining an unknown genotype in a dominant person. It is a breeding procedure in which a (known genotype) homozygous recessive individual is paired with an individual of the opposite mating type who has an unknown dominant genotype. The phenotypic characteristics of the resulting children are investigated, and the genotype of the examined individual is determined appropriately. If all of the progeny from the test cross are dominant, we may conclude that the genotype of the tested unknown person is homozygous dominant. If 50% of kids exhibit dominant traits and the remaining 50% exhibit recessive traits, we may conclude that the genotype of the tested unknown individual is heterozygous dominant. #genetics #backcross #testcross #mendel #crosses #monohybridtestcross #backcrossandtestcross #typesofcross #dihybridtestcross #limitationsofcross #applicationsofcross #mscbotany #botany

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Wepresent Atacama Large Millimeter/submillimeter Array 12-m, 7-m, and Total Power Array observations of the FUOrionis outbursting system, covering spatial scales ranging from 160 to 25,000 au. The high-resolution interferometric data reveal an elongated 12CO(2–1) feature previously observed at lower resolution in 12CO(3–2). Kinematic modeling indicates that this feature can be interpreted as an accretion streamer feeding the binary system. The mass infall rate provided by the streamer is significantly lower than the typical stellar accretion rates (even in quiescent states), suggesting that this streamer alone is not massive enough to sustain the enhanced accretion rates characteristic of the outbursting class prototype. The observed streamer may not be directly linked to the current outburst, but rather a remnant of a previous, more massive streamer that may have contributed enough to the disk mass to render it unstable and trigger the FU Orionis outburst. The new data detect, for the first time, a vast, slow-moving carbon monoxide molecular outflow emerging from this object. To accurately assess the outflow properties (mass, momentum, and kinetic energy), we employ 13CO(2–1) data to correct for optical depth effects. The analysis indicates that the outflow corresponds to swept-up material not associated with the current outburst, similar to the slow molecular outflows observed around other FUor and Class I protostellar objects.

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The presentation first gives the brief introduction about the animal communication covering the reasons for communication. It then describes the important types of animal communication emphasizing the important points regarding auditory and visual communication. With respect to auditory communication, it covers the uses of it and mechanisms of auditory communication along with examples. Later the presentation discusses the various forms of visual communication such as gestures, postures, facial expressions, color changes , bioluminescent communication. It then describes the visual communication in bees in the form of bee dance . In bee dance it gives a brief overview of round dance , sickle dance and waggle dance .

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Small size (20-300 nm) / bacteria (size measured in μm) Viral genome – single type of nucleic acid: DNA – dezoxyriboviruses RNA - riboviruses Totally dependant on living cells for all metabolic processes May infect humans, animals, plants, bacteria, fungi,parasites, insects Virion = viral corpuscle = elementary, infectious unit. Only visible by electron microscopy. Laboratory diagnosis of viral infections Methods: • Cytology • Electron microscopy • Cultivation • Detection of viral proteins • Serology • Molecular diagnosis (detection of genetic material i.e. nucleic acids) PPT prepared by Dr. Prince C P

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Cotton crops are vulnerable to a variety of sucking pests, which can severely impact plant health, yield, and fiber quality. These pests primarily feed on plant sap, extracting nutrients directly from the plant's vascular system. Here's a breakdown of some of the most significant sucking pests in cotton cultivation: Aphids: Cotton aphids or melon aphids can cause direct damage by sucking sap and indirect damage by secreting honeydew, which encourages sooty mold growth. This can interfere with photosynthesis and weaken the plant. Aphids can also transmit viral diseases. Whiteflies: Two species, the silverleaf whitefly and the bandedwinged whitefly, are particularly troublesome. They not only suck sap from the underside of leaves, causing yellowing and leaf drop, but their honeydew excretion promotes sooty mold and they can transmit several plant viruses. Thrips: While thrips can chew on plants, their primary damage to cotton is through sucking. They attack the cotton plant during its seedling stage, which can stunt growth and reduce vigor. Thrips are also capable of transmitting the Cotton Bud disease. Spider Mites: These are not insects but arachnids. Spider mites, such as the two-spotted spider mite, suck cell contents from the leaves, leading to speckled discoloration and potentially significant leaf loss if infestations are severe. Leafhoppers: Including various species, leafhoppers can cause direct damage through feeding, which results in leaf curling and stunted growth. They can also be vectors for plant diseases. Mealybugs: These pests are less common but can be problematic, especially in clustered planting conditions. They suck sap and secrete honeydew, which leads to sooty mold. Mealybugs can also spread viruses. Stink Bugs: Although primarily known for their chewing mouthparts, certain stink bugs can cause damage similar to sucking pests by injecting saliva into the plant and sucking out nutrients, leading to boll damage and stained lint. Management Strategies: Cultural Controls: This includes practices such as crop rotation, using resistant varieties, and managing planting and harvesting times to avoid peak pest populations. Biological Controls: Beneficial insects like lady beetles, lacewings, and predatory mites can naturally control sucking pest populations. Parasitic wasps also play a role in controlling aphid and whitefly populations. Chemical Controls: Insecticides may be used but should be chosen carefully to minimize resistance development and preserve beneficial insects. Systemic insecticides can be particularly effective against sucking pests. Integrated Pest Management (IPM): Combining multiple control strategies based on monitoring and thresholds to apply the most effective and environmentally sensitive approach. Effective management of sucking pests in cotton requires a thorough understanding of the pest species present, their life cycles, and the ecological balance of the field environment.

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Flow Imaging Microscopy Equipment

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