Em shockage
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Bacteriophage Shockage was isolated and characterized. Electron microscopy pictures were taken of the phage particles. The pictures show the structure of Shockage, which includes an icosahedral capsid and long, non-contractile tail.
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Bacteriophage Zombage was isolated and characterized using electron microscopy. Pictures were taken of the phage using electron microscopy to view its structure and morphology at high magnification. The electron microscopy images provide detailed views of Zombage's capsid, tail fibers, and other viral structures.
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1. This laboratory focused on practicing proper micropipetting techniques. Students mixed solutions of water and dyes to practice using micropipettes accurately and safely. This helped prepare them for upcoming labs requiring precise measurements.
2. Students learned different microscopy techniques, including fluorescence and bright field microscopy. They took photomicrographs using a camera attached to the microscope.
3. Over three days, students extracted their own DNA from mouth cells, ran PCR tests to determine if a patient sample had diabetes, and used gel electrophoresis to analyze protein biomarkers and diagnose a lysosomal storage disorder.
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This document describes the isolation of mycobacteriophages from soil samples in Puerto Rico. Two students isolated three unique phages and characterized them through plaque assays, enrichment techniques, and SDS-PAGE gel electrophoresis. Two distinct phages, named Shockage and Zombage, were identified based on their protein band patterns and plaque morphology. Further sequencing of the phage genomes was proposed to better characterize the newly discovered viruses.
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Lab summaries complete
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2. Students learned different microscopy techniques, including fluorescence and bright field microscopy. They took photomicrographs using a camera attached to the microscope.
3. Over three days, students extracted their own DNA from mouth cells, ran PCR tests to determine if a patient sample had diabetes, and used gel electrophoresis to analyze protein biomarkers and diagnose a lysosomal storage disorder.
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- 1. Electron Microscopy Pictures of Isolated and Characterized Bacteriophage: Shockage