ELISA protocol poultry

1. ELISA poultry 1

2. ELISA poultry 2

3. •ELISA is susceptible to time, temperature, light,
movement and performance of protocol, so there
are many influencers on the outcome of the test.
Assessing sample results only will not give
guarantee the results are valid.
•Avoid pipetting small volumes: adding 1 ul serum
to 500 ul diluent comes with great risk of being
inaccurate and in precise
ELISA poultry 3

4. •To increase accuracy and precision, it is
recommended to split the sample dilution into
2 steps.
– Step 1: 5 ul sample and add 245 ul diluent
(1:50)
– Step 2: 90 ul diluent and add 10 ul of 1:50
diluted samples (1:10)
•1:50 x 1:10 = 1:500
•By reducing test variation, it allows the
veterinarian to do more meaningful
interpretation of the results
•Get reliable results:
– Correct method
– Good technique
– Good equipment
– Reference control
ELISA poultry 4