![D is ta n c e a lo n g Y a x is (m m )
GlucoseConcentration(mM)
0 1 0 2 0 3 0 4 0
0
1
2
3
4
5
1 5
1 6
1 7
1 8
1 9
2 0
Y0 Y
(F)
Ex Vivo Bovine 20 mM
Human Degenerated
D is ta n c e a lo n g X a x is (m m )
GlucoseConcentration(mM)
0 1 0 2 0 3 0
0
1
2
3
4
5
1 5
1 6
1 7
1 8
1 9
2 0 H u m a n H e a lth y
H u m a n D e g e n e ra te d
E x V iv o B o vin e 5 m M
E x V iv o B o vin e 2 0 m M
Ex V iv o B o vin e S e a le d
o ff C E P
X
AF NP
CEP
X0
X
Day7Day0
RESULTS - GLUCOSE CONCENTRATION MAP
Ex Vivo Bovine 5 mM
X0
X
X0
X
Y0
Y
Y0
Y
Y0
Y
Ex Vivo Bovine 5 mM
0 mM
2 mM
3 mM
4 mM
5 mM
1 mM
17 mM
18 mM
19 mM
20 mM
16 mM
X0
X
Y0
Y
Human Healthy Human Healthy
Human Degenerated
Ex Vivo Bovine 20 mM
Ex Vivo Bovine Sealed Off CEP Ex Vivo Bovine Sealed Off CEP
15 mM
X0
X
Y0
Y
0 mM
2 mM
3 mM
4 mM
5 mM
1 mM
0 mM
2 mM
3 mM
4 mM
5 mM
1 mM
0 mM
2 mM
3 mM
4 mM
5 mM
1 mM
X0 X
HeightofIVD(mm)
D a y 0 D a y 7
0
1 0
2 0
3 0
Day 7 Control
RESULTS - ONE WEEK DEFECT MODEL STUDY
MATERIALS & METHODS
Day 7 Defect Chamber
IVD
Isolation
IVD cultured for 7 days in
defect chamber with CEP
sealed off
Stirring essential to ensure no gradients
Computational model to map glucose
concentrations using Comsol ®
INTRODUCTION
Degeneration of the intervertebral disc (IVD) is considered as
a primary cause to back pain, which affects up to 80% of
people at some stage during their life costing €240 billion in
Europe annually.
Healthy
IVD
Early Stage
Degeneration
Late Stage
Degeneration
Nucleus Pulposus (NP)
Annulus Fibrosus (AF)
Cartilage End Plate
(CEP)
Transportation of vital nutrients, such as glucose play a
significant role in maintaining disc health. Disc degeneration
results in a loss of function and reduction in overall disc height.
CURRENT TREATMENTS
[2]
Disc replacement
Injectable cell based
therapy
OBJECTIVES
Develop a static organ culture system to induce degeneration
in a bovine IVD and to characterise the glucose nutrient
microenvironment that exists in ex vivo organ culture models.
DISCUSSION & CONCLUSION
This work was supported by the MSc in Bioengineering programme at Trinity College Dublin, Ireland.
TRINITY CENTRE FOR BIOENGINEERING
Y
Z
X
Y
Z
Y
X
Y
Z
Cell Death
Begins at
0.5 mM [3]
Computational models show that by sealing of the CEP cell death should
occur within the NP. A defect was formed within NP and change in disc
height was observed after a 1 week experimental study in defect chamber.
Future experiments will analyse intradiscal pressure and cell viability, as
well as experimentally determining the glucose concentration.
Current culture techniques may not truly represent the physiological
conditions within the IVD and therefore need to be revised in order to
validate injectable cell based therapies.
[1] Galbusera, F. 2011. Computer Methods in Biomechanics and Biomedical Engineering, 14(8), pp. 729-39.
[2] http://www.privatepatientadvocate.com/images/ArtificialDisc300.jpg
[3] Horner, H. A. & Urban, J. P. 2001. Spine, 26(23), pp. 2543-2549.
ACKNOWLEDGMENTS & REFERENCES
[1]](https://image.slidesharecdn.com/padraigirwinposterforexternalsjune19th-190615172413/85/Dissertation-Poster-for-Trinity-College-Dublin-1-320.jpg)
Recommended
Recommended
More Related Content
Similar to Dissertation Poster for Trinity College Dublin
Similar to Dissertation Poster for Trinity College Dublin (20)
Recently uploaded
Recently uploaded (20)
Dissertation Poster for Trinity College Dublin
- 1. D is ta n c e a lo n g Y a x is (m m ) GlucoseConcentration(mM) 0 1 0 2 0 3 0 4 0 0 1 2 3 4 5 1 5 1 6 1 7 1 8 1 9 2 0 Y0 Y (F) Ex Vivo Bovine 20 mM Human Degenerated D is ta n c e a lo n g X a x is (m m ) GlucoseConcentration(mM) 0 1 0 2 0 3 0 0 1 2 3 4 5 1 5 1 6 1 7 1 8 1 9 2 0 H u m a n H e a lth y H u m a n D e g e n e ra te d E x V iv o B o vin e 5 m M E x V iv o B o vin e 2 0 m M Ex V iv o B o vin e S e a le d o ff C E P X AF NP CEP X0 X Day7Day0 RESULTS - GLUCOSE CONCENTRATION MAP Ex Vivo Bovine 5 mM X0 X X0 X Y0 Y Y0 Y Y0 Y Ex Vivo Bovine 5 mM 0 mM 2 mM 3 mM 4 mM 5 mM 1 mM 17 mM 18 mM 19 mM 20 mM 16 mM X0 X Y0 Y Human Healthy Human Healthy Human Degenerated Ex Vivo Bovine 20 mM Ex Vivo Bovine Sealed Off CEP Ex Vivo Bovine Sealed Off CEP 15 mM X0 X Y0 Y 0 mM 2 mM 3 mM 4 mM 5 mM 1 mM 0 mM 2 mM 3 mM 4 mM 5 mM 1 mM 0 mM 2 mM 3 mM 4 mM 5 mM 1 mM X0 X HeightofIVD(mm) D a y 0 D a y 7 0 1 0 2 0 3 0 Day 7 Control RESULTS - ONE WEEK DEFECT MODEL STUDY MATERIALS & METHODS Day 7 Defect Chamber IVD Isolation IVD cultured for 7 days in defect chamber with CEP sealed off Stirring essential to ensure no gradients Computational model to map glucose concentrations using Comsol ® INTRODUCTION Degeneration of the intervertebral disc (IVD) is considered as a primary cause to back pain, which affects up to 80% of people at some stage during their life costing €240 billion in Europe annually. Healthy IVD Early Stage Degeneration Late Stage Degeneration Nucleus Pulposus (NP) Annulus Fibrosus (AF) Cartilage End Plate (CEP) Transportation of vital nutrients, such as glucose play a significant role in maintaining disc health. Disc degeneration results in a loss of function and reduction in overall disc height. CURRENT TREATMENTS [2] Disc replacement Injectable cell based therapy OBJECTIVES Develop a static organ culture system to induce degeneration in a bovine IVD and to characterise the glucose nutrient microenvironment that exists in ex vivo organ culture models. DISCUSSION & CONCLUSION This work was supported by the MSc in Bioengineering programme at Trinity College Dublin, Ireland. TRINITY CENTRE FOR BIOENGINEERING Y Z X Y Z Y X Y Z Cell Death Begins at 0.5 mM [3] Computational models show that by sealing of the CEP cell death should occur within the NP. A defect was formed within NP and change in disc height was observed after a 1 week experimental study in defect chamber. Future experiments will analyse intradiscal pressure and cell viability, as well as experimentally determining the glucose concentration. Current culture techniques may not truly represent the physiological conditions within the IVD and therefore need to be revised in order to validate injectable cell based therapies. [1] Galbusera, F. 2011. Computer Methods in Biomechanics and Biomedical Engineering, 14(8), pp. 729-39. [2] http://www.privatepatientadvocate.com/images/ArtificialDisc300.jpg [3] Horner, H. A. & Urban, J. P. 2001. Spine, 26(23), pp. 2543-2549. ACKNOWLEDGMENTS & REFERENCES [1]