This document discusses research activities related to oxygen metabolism and regenerative medicine in horses being conducted by the Equine Teaching Hospital, Equine Research Center of Mont-le-Soie, and Centre For Oxygen, R&D at the University of Liege in Belgium. The research topics include laminitis, colic, mitochondrial function, oxidative stress, and regenerative medicine using stem cells and other therapies. The groups are conducting clinical trials and research on conditions such as developmental orthopaedic disease, tendinopathy, arthropathy, and muscle disorders using equipment like a treadmill, gait analyzer, and oxygen consumption monitors. Patents have been filed for stem cell therapies, anti-inflammatory drugs, and diagnostic tools.

1. Oxygen Metabolism and
Regenerative Medicine in Horses
D. Serteyn et al.
Research activities in horses
- Equine Teaching Hospital
- Equine Research Center of Mont-le-Soie
- Centre For Oxygen, R&D
Contact: didier.serteyn@ulg.ac.bet

2. Oxygen Metabolism and Regenerative Medicine in Horses
Equine Teaching Hospital
2ULg – FMV – FARAH – Médecine vétérinaire comparée
The Equine Hospital is collaborating with
associated laboratories and research groups in
order to increase the knowledge of equine
diseases, as well as their treatment and
prevention. Research topics include:
•laminitis
•colic and systemic inflammatory response
syndrome
•mitochondrial function and its modification
•Oxidative stress
•Regenerative medicine
The Equine Hospital is also able to lead “clinical
Trials” to assess new therapeutic approach (Stem
Cells, NDS27)

3. Oxygen Metabolism and Regenerative Medicine in Horses
Equine Research Center of Mont-le-Soie
3ULg – FMV – FARAH – Médecine vétérinaire comparée
For Equine investigation on orthopaedic disease: JPH
Lejeune (PhD), I. Caudron (ECVS, PhD), L. Mendoza
(PhD Student), V. Libertiaux (PhD, engeneer)
Equipment: treadmill, gait analyser, cinematic 3D,
Pressure sensors, Keegan Lameness Locator
Thematic: Developmental Orthopaedic Disease
(Osteochondrosis), biomechanical approach in
tendinopathy and arthropathy

4. Oxygen Metabolism and Regenerative Medicine in Horses
Center For Oxygen, R&D (CORD)
4ULg – FMV – FARAH – Médecine vétérinaire comparée
Interdisciplinary Research Unit: Physiopathology of Reactive Oxygen Species
• 20 EFT from different disciplines and departments (medicine, pharmacy, physic, biology, ….)
For Equine Research: T. Franck (PhD), A. Mouythis-Mickalad (PhD), J. Ceusters (PhD), S. Derochette (PhD
student)
• Inflammation and ROS generation from activated neutrophils: Myeloperoxidase, NaDPH Oxidase,
NDS27
• Mitochondrial dysfunction, ROS generation and ATP production: High Resolution Oxygraphy,
Resonance Paramagnetic Electronic
• Muscle derived Mesenchymal Stem cells: characterisation, differentiation, role of oxygen and
mitochondria
Patents:
• Specific Immuno Extraction followed by enzymatic Detection (SIEFED)
Delivered for myeloperoxidase
• Hydro-soluble form of Curcumin (NDS27)
• Transcryptomic diagnosis of OCD
• Muscle derived mesenchymal stem cells

5. Oxygen Metabolism and Regenerative Medicine in Horses
Center For Oxygen, R&D (CORD)
5ULg – FMV – FARAH – Médecine vétérinaire comparée
• Inflammation and ROS generation from activated neutrophils: Myeloperoxidase, NaDPH
Oxidase, NDS27
(WO/2009/144220) WATER SOLUBLE CURCUMIN
COMPOSITIONS FOR USE IN ANTI-CANCER AND
ANTI-INFLAMMATORY THERAPY
(Neven P. , Serteyn D. et coll.)  NDS27
NDS27 free radical scavenger and inhibitor
Of MPO and Nox2
Licence to BiopTis (Spin Off)
Clinical trial is underway

6. Oxygen Metabolism and Regenerative Medicine in Horses
Center For Oxygen, R&D (CORD)
6ULg – FMV – FARAH – Médecine vétérinaire comparée
Mitochondrial dysfunction: metabolic syndrome, myopathy, laminitis,…
Specific tools to assess bioenergetics profile and metabolism.
• High Resolution Oxymetry (HRO): use for O2 consumption rate (OCR), Maximal capacity
respiratory (MCR) and OXPHOS capacity (D. Votion, J. Ceusters, A. Mouythis-Mickalad)
• Electron Paramagnetic Resonance (EPR or ESR): detection of free radicals
• Fluorescence: intracellular detection of ROS generation
• Cell viability, ATP production, cytochrome C release, …
Oxygen consumption
Fitness evaluation

7. Oxygen Metabolism and Regenerative Medicine in Horses
Center For Oxygen, R&D (CORD)
7ULg – FMV – FARAH – Médecine vétérinaire comparée
Muscle derived Mesenchmal Stem Cells (Patent D. Serteyn, J. Ceuters)
-
Less invasive method  Bio-Banking of autologous mdMSC
Recent regional and international RD programs
- Differentiation in several types of cells (CER, CORD)
- Clinical trial on tendinitis (MLS, Equine clinic)
- Role of mitochondria in the differentiation steps (CORD)
- Combination mdMSC/Scaffold
- Eurostars project: Ulg/Revatis/Utwente/Hy2care
- University of porto
- University of Texas A&M
- CORD/GIGA: cardiac cells on a porcine model of heart valves
Licenced to REVATIS (Spin Off Ulg)
International development: Dubaï, College-Station,
Hong Kong …. Japan