Somatic cell nuclear transfer is a technique used for cloning. It involves removing the nucleus from an egg cell, known as an oocyte, and replacing it with the nucleus of a donor adult cell. The oocyte is then stimulated to divide and develop into an embryo. Precise steps are followed, including aligning the egg spindle with a pipette, enucleating the oocyte, inserting the donor cell nucleus into the perivitelline space of the oocyte, and stimulating division. The goal is to generate cloned embryonic stem cells for research or medical applications.

1. Clonación cell stem

2. http://www.abc.es/salud/noticias/celulas-piel-humanas-reprogramadas-convierten-14925.html

3. http://estaticos.elperiodico.com/resources/pdf/1/0/1368652108701.pdf

5. Somatic cell nuclear transfer
Align spindle and enucleatión pipette by rotating oocyte

6. Hold oocyte tightly make sure alignment by gently pushing the spindle with pipette tip laser assisted zona drilling
Gently insert pipette and aspirate spindles with….
small amount of cytoplast, as much as possible….
donor cell handling
Briefly soak donor cell into sev extract
donor cell pickup from sev extract
position spindle free oocyte
laser assisted zona drilling
injection donor cell into perivitelline aspace (NT)