BioPharmaceutical Protein Characterization | Pharmafocusasia

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kevin brown

Get greater structural insight into your drug product with our biopharmaceutical protein characterization workflows

Health & Medicine

Subunits analysis approach for the determination of fucosylation
levels mAbs
Learn middle-up quantifications of mAb core afucosylation and high-mannose type
glycans
See applicability of Thermo Scientific™ MAbPac™ RP columns for mAb subunits
analysis
Learn benefits of Thermo Scientific™ Q Exactive™ BioPharma platform of mass
spectrometers for accurate-mass MS analysis on different domains of monoclonal
antibodies
Subunit, intact and native mass spectrometry
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IdeS-cleaved mAb subunit analysis with LC-HRAM-MS: a quick and
accurate comparison of biosimilar and originator biotherapeutics
Learn middle-up approach benefits for biotherapeutic characterization
See applicability of Thermo Scientific™ MAbPac™ RP columns for mAb subunits
analysis
Learn benefits of Thermo Scientific™ Q Exactive™ BioPharma platform of mass
spectrometers for accurate-mass MS analysis on different domains of monoclonal
antibodies
Large molecule bio-analysis
A fast and simple workflow for surrogate peptide bioanalysis:
NISTmAb case study
Fast and simple workflow for the quantitative analysis of monoclonal antibodies with
96 samples manually processed in under four hours
Highly reproducible immunocapture & tryptic digestion
Selective and sensitive analysis with LLOQ of 10 ng/mL from just 50 μL of sample.
Free Insights
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Protein qualitative analysis based on mass spectrometry explores protein expression within organisms. Mass spectrometry offers highly efficient, robust, and accurate results and is one of the core technologies for proteomic research. Protein identification is a common topic for biochemistry research, and mass spectrometry is considered one of the most useful techniques that solve this issue. Two major strategies that are widely used for protein identification by mass spectrometry are MALDI-TOF-based protein fingerprinting and LC-MS/MS-based peptide sequencing. Meanwhile, LC-MS/MS reserved higher sensitivity and ability than MALDl-TOF and can accurately identify multiple protein components from a single sample. https://www.creative-proteomics.com/services/protein-identification.htm

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The core AIM of the present study is to develop a novel, rapid, precise and accurate RP-HPLC method for simultaneous separation and quantification of six fluoroquinolones OF LEVOFLOXACIN (LEVO), PRULIFLOXACIN (PRFX), GATIFLOXACIN (GATI), SPARFLOXACIN (SPAR), MOXIFLOXAXIN (MOXI) AND BALOFLOXACIN (BALO) for the the day to day analysis. The author felt that a novel single method for separation and quantification of all the above said drugs on single chromatographic system without any minor changes in detection wavelength and mobile phase composition. To develop rapid, sensitive and economical analytical method based on HPLC for separation and estimation of six fluoroquinolones pharmaceutical dosage forms. To develop method with shorter run time and better sensitivity. Reducing the solvent consumption to make it more eco-friendly. Avoid the column damage by minimizing the buffer strength and pH of mobile phase than reported methods. To validate the method for different parameters like Accuracy, Precision, Linearity, specificity, Robustness International Conference on Harmonization ICH Q2(R1) guidelines.. To apply the developed RP-HPLC method in the analysis of pharmaceutical formulations.

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