This document shows the effect of temperature on microbial growth and color change. It provides data showing that microbial growth rate increases with temperature from 10 to 30 degrees Celsius, peaks at 30 degrees, and then declines as temperature continues to rise from 35 to 50 degrees. The color of the microbial culture also changes with temperature, shifting from blue to purple to pink to colorless and then back to purple and blue again as the temperature increases from 10 to 50 degrees Celsius.