basic flowcytometry 11111111111111111111
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flowcytometry basic priciple
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basic flowcytometry 11111111111111111111
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Editor's Notes
- Flow = Motion Cyto = Cell Metry = Measurement
- After hydrodynamic focusing, each cell passes through a beam of light.
- The number of detectors will vary according to the machine.
- A particular colour of light is split off from the incoming mixture and directed to the detectors by dichroic mirrors.
- ‘Y’ = Counts ‘X’ = Fluorescence Intensity Subpopulations are defined as peaks in the histogram. FITC – Fluorescein isothiocyanate dye
- Cd45 all most all cell of the immunological and hematological system except mature rbc n platelets.
- Most commonly used cell sorting method is electrostatic deflection of droplets.
- In addition to surface immunophenotyping and cytoplasmic characterization, flow cytometry is also used in cell cycle analysis. Stoichiometric means the stain is directly proportional to the amount of DNA within the cell.
- PI = Propidium iodide