Bacterial display

•

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The protein or peptide library is constructed using recombinant DNA technology and displayed on bacteria. The bacterial library can be labeled with fluorescently labeled target protein (antigen), or incubated with biotin-tagged target protein and streptavidin-magnetic beads.

Health & Medicine

Bacterial display systems1
© 2007 - 2018 Creative-Biolabs All Rights Reserved Email: info@creative-biolabs.com Tel: 1-631-381-2994
BACTERIAL DISPLAY TECHNOLOGY
Multiple cell surface display
platforms on bacteria have
been established. For display
on Gram-negative bacteria,
insertion scaﬀolds (based on
OmpA, OmpX, FhuA, FliTrx,
FimH), N-terminal display
scaﬀolds (based on EstA,
AIDA-I, IgA protease), C-ter-
minal display scaﬀolds (based
on Lpp-OmpA, Invasin), and
combination of N-terminal
and C-terminal display using
circularly permuted OmpX
(CPX) could be chosen. For
Gram-positive bacteria,
foreign proteins could be
displayed via covalently
anchored proteins including
SPA and FnBTB.
The protein or peptide library
is constructed using recombi-
nant DNA technology and
displayed on bacteria. The
bacterial library can be
labeled with ﬂuorescently
labeled target protein
(antigen), or incubated with
biotin-tagged target protein
and streptavidin-magnetic
beads. Target-binding bacte-
ria are isolated using ﬂuores-
cence-activated cell sorting
(FACS) or magnetic-activated
cell sorting (MACS). The
selection cycle is generally
repeated, and selected bacte-
ria are typically ampliﬁed by
growth in between the cycles.
Finally, isolated binders are
screened using whole-cell
ELISA-based methods, identi-
ﬁed using DNA sequencing
and subcloned for soluble
production and detailed
characterization.
Construction and screening of display library2
Bacterial Display Services
Bacterial Display Library Construction
Epitope Mapping by Bacterial Display
Cell-Binding Peptides Identification
Protease Substrate Identification
Protein-Binding Ligand Screening
Related Services
Ribosome Display Services
Yeast Display Services
Mammalian Cell Display Service
Phage Display & Antibody Library Service
WHAT WE DO:
Creative Biolabs
Bacterial Display
Services
Library DNA
Plasmid constructsFACS
Cloning
MACS
AmplificationAmplification
E. coli
Fluorescently
labeled reporter protein
Lower-affinity
variants Lower-affinity
variants
Highest-affinity
variant
Highest-affinity
variant
Display library Biotin-tagged
reporter protein &
streptavidin-magnetic beads
G
-
bacteria
N-terminal
display
scaffolds:
EstA
AIDA-I
IgA protease
G
-
bacteria
N-terminal
display
using
CPX
G
-
bacteria
C-terminal
display
using
CPX
G
-
bacteria
C-terminal
display
scaffolds:
Lpp-OmpA
Invasin
G+
bacteria:
display via
covalently
anchored
proteins
SPA
FnBTB
G
-
bacteria
Insertion
scaffolds:
OmpA
OmpX
FhuA
FliTrx
FimH
N
C
N
C
N
C
N C N C
N C

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